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The map label for this gene is proS

Identifier: 78778894

GI number: 78778894

Start: 475841

End: 477643

Strand: Reverse

Name: proS

Synonym: PMT9312_0509

Alternate gene names: 78778894

Gene position: 477643-475841 (Counterclockwise)

Preceding gene: 78778900

Following gene: 78778893

Centisome position: 27.95

GC content: 32.78

Gene sequence:

>1803_bases
ATGCGAGTAACCACCTCATTTCCTCTGGGGACACTTCGTGACACACCTTCTGAAGCTGAGATTATTTCACATCAATTACT
TTTAAAAGCTGGTTACATTCGCAGAGTTTACAGCGGCATTTATGCATACATGCCAATAATGCTTAAAGTTATTGAGAAAA
TATCTAAAATAATAGAGATAGAACTCAATAGTATTGGTTGCACAAAATTACTCTTACCTCAACTTCATCCTGCAAATTTA
TGGAAAAAAAGTGAAAGGTGGGAAGGATATACTGCAGGGGAAGGAATAATGTTTAATCTAAAAGACAGACAAGGGAAAGA
ATTTGGTTTAGCTCCAACTCACGAAGAGGTGATAACAAGTATTGCATCAGAGATCATCAACTCCTATAAGCAATTACCTC
AATGTTTTTACCAAATTCAGACAAAATTTAGAGATGAAATAAGGCCAAGATTTGGATTAATGAGGAGTAGAGAATTTATA
ATGAAAGATGGTTATTCTTTCCATTCTTCAGAAAAAGACCTGGCTTCATTTTATGAAAAGGTGGGCAATGCTTACGAAAA
TATTTTTAAATCTTGTGGACTAGAGACAGTAGGGGTTGAAGCAGATAGTGGAGCCATTGGCGGGGCCTCCTCCAAAGAAT
TTATGGTAACTGCAGATGCTGGGGAAGACTCCATCTTGTTTACTCAAAGCGGTTCATATGCTGCGAATATTGAAAAAGCT
GTTTCCATTCCCTCTCAACCTATTCCATTAAAAAATAATATTTCAGGATGGATAGAAACACCTGAACAAAAAACAATTCT
CGAAGTTTGTAAAAATAATAATTTAGACCCTAGTCAGATTATTAAAGTCGTAGTATTTCTTGCAAAGTTTGAGATTAAAT
CGGAGGTCCCAATTCTTGCTTGCATAAGAGGAGATCAACATATTAATGAAGTAAAGCTTTTTAACTTAATAAATAAACTT
CATAGTTCCAACCTCCTGCATCTTCAGAAAATTGAAGACATAAAAATTATCGAAAAAAACCTAGTTAACTTTCCTTTAGG
TTTTATCGGGCCAGACCTAGATAATGAAACTATTAAAGCGAGTTCTAATTGGGACAAAACATGGACGAGAATAATTGATC
ATTCTGCAAGTACTCTCTCAAAGTTTATAAGTGGTGCGAATAAAGTTAATTTCCACAAAGTTTTTCAGGAGTTTTCTTTT
ACTTCAAAAGATTATCTAATAGAGGATATTAGGAATGCTAAAAAAGGAGATAAAATAAAAATCAATGATAATGAAGAACT
CAAAGAAAAAAAAGGTATTGAGATAGGACATATTTTTCAACTAGGACAAAAATATAGTGAAAAATTAAATGCTAAGTTTT
CTGATAAGGATGGTCATTTGAAAAATTTATGGATGGGTTGTTATGGAATTGGAGTAACAAGAATAGCTCAAGCTGCTATT
GAACAGAATCATGATCAAAAAGGAATTTGTTGGCCGATCCAGATTTCTCCTTTTGAAGTCATTATTATTCCAACCAACCT
AAAAGATCAGATTCAAAGAGACCTTACAGAGCAAATATATAACAACTTTTTAGTTAATAAAATTGACGTTCTTCTTGATG
ACAGGGAAGATAGAGCGGGCGTGAAATTCAAAGATGCAGAATTAATTGGAATTCCTTTTCAAATAATTATTGGTAGAGAT
TCTATTAACAACGAAGTAGAACTTATATGCAGAACAAATAATACTAAATTTAAAATTAGTGCCCATAAATTGTTGGAAAC
ATTTATTTCCGAATCAGAAATAATGTACAATAAAAATTCTTAA

Upstream 100 bases:

>100_bases
AAGAATATTTCAAGACTAGCGGCGTTGAGGGACTCAAGACACTTTTTATTTTTTTTTCATCTTAATTGATAAAATTAACT
ATTATTAAATTATTTTTTGT

Downstream 100 bases:

>100_bases
GGCAAAATTTTTGGGAGCTAATTATGTTACTAAAATGGACATCAGAATTAATTATTAAAAATCTCACCAAAGCTATATCT
TTTTCACTCTCCTTTATTGT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MRVTTSFPLGTLRDTPSEAEIISHQLLLKAGYIRRVYSGIYAYMPIMLKVIEKISKIIEIELNSIGCTKLLLPQLHPANL
WKKSERWEGYTAGEGIMFNLKDRQGKEFGLAPTHEEVITSIASEIINSYKQLPQCFYQIQTKFRDEIRPRFGLMRSREFI
MKDGYSFHSSEKDLASFYEKVGNAYENIFKSCGLETVGVEADSGAIGGASSKEFMVTADAGEDSILFTQSGSYAANIEKA
VSIPSQPIPLKNNISGWIETPEQKTILEVCKNNNLDPSQIIKVVVFLAKFEIKSEVPILACIRGDQHINEVKLFNLINKL
HSSNLLHLQKIEDIKIIEKNLVNFPLGFIGPDLDNETIKASSNWDKTWTRIIDHSASTLSKFISGANKVNFHKVFQEFSF
TSKDYLIEDIRNAKKGDKIKINDNEELKEKKGIEIGHIFQLGQKYSEKLNAKFSDKDGHLKNLWMGCYGIGVTRIAQAAI
EQNHDQKGICWPIQISPFEVIIIPTNLKDQIQRDLTEQIYNNFLVNKIDVLLDDREDRAGVKFKDAELIGIPFQIIIGRD
SINNEVELICRTNNTKFKISAHKLLETFISESEIMYNKNS

Sequences:

>Translated_600_residues
MRVTTSFPLGTLRDTPSEAEIISHQLLLKAGYIRRVYSGIYAYMPIMLKVIEKISKIIEIELNSIGCTKLLLPQLHPANL
WKKSERWEGYTAGEGIMFNLKDRQGKEFGLAPTHEEVITSIASEIINSYKQLPQCFYQIQTKFRDEIRPRFGLMRSREFI
MKDGYSFHSSEKDLASFYEKVGNAYENIFKSCGLETVGVEADSGAIGGASSKEFMVTADAGEDSILFTQSGSYAANIEKA
VSIPSQPIPLKNNISGWIETPEQKTILEVCKNNNLDPSQIIKVVVFLAKFEIKSEVPILACIRGDQHINEVKLFNLINKL
HSSNLLHLQKIEDIKIIEKNLVNFPLGFIGPDLDNETIKASSNWDKTWTRIIDHSASTLSKFISGANKVNFHKVFQEFSF
TSKDYLIEDIRNAKKGDKIKINDNEELKEKKGIEIGHIFQLGQKYSEKLNAKFSDKDGHLKNLWMGCYGIGVTRIAQAAI
EQNHDQKGICWPIQISPFEVIIIPTNLKDQIQRDLTEQIYNNFLVNKIDVLLDDREDRAGVKFKDAELIGIPFQIIIGRD
SINNEVELICRTNNTKFKISAHKLLETFISESEIMYNKNS
>Mature_600_residues
MRVTTSFPLGTLRDTPSEAEIISHQLLLKAGYIRRVYSGIYAYMPIMLKVIEKISKIIEIELNSIGCTKLLLPQLHPANL
WKKSERWEGYTAGEGIMFNLKDRQGKEFGLAPTHEEVITSIASEIINSYKQLPQCFYQIQTKFRDEIRPRFGLMRSREFI
MKDGYSFHSSEKDLASFYEKVGNAYENIFKSCGLETVGVEADSGAIGGASSKEFMVTADAGEDSILFTQSGSYAANIEKA
VSIPSQPIPLKNNISGWIETPEQKTILEVCKNNNLDPSQIIKVVVFLAKFEIKSEVPILACIRGDQHINEVKLFNLINKL
HSSNLLHLQKIEDIKIIEKNLVNFPLGFIGPDLDNETIKASSNWDKTWTRIIDHSASTLSKFISGANKVNFHKVFQEFSF
TSKDYLIEDIRNAKKGDKIKINDNEELKEKKGIEIGHIFQLGQKYSEKLNAKFSDKDGHLKNLWMGCYGIGVTRIAQAAI
EQNHDQKGICWPIQISPFEVIIIPTNLKDQIQRDLTEQIYNNFLVNKIDVLLDDREDRAGVKFKDAELIGIPFQIIIGRD
SINNEVELICRTNNTKFKISAHKLLETFISESEIMYNKNS

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=229, Percent_Identity=43.2314410480349, Blast_Score=191, Evalue=2e-48,
Organism=Escherichia coli, GI1786392, Length=592, Percent_Identity=41.3851351351351, Blast_Score=443, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI115532348, Length=212, Percent_Identity=34.9056603773585, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI71991342, Length=179, Percent_Identity=27.3743016759777, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6320931, Length=557, Percent_Identity=29.443447037702, Blast_Score=218, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24656200, Length=226, Percent_Identity=37.6106194690265, Blast_Score=160, Evalue=3e-39,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_PROM9 (Q31C25)

Other databases:

- EMBL:   CP000111
- RefSeq:   YP_397006.1
- ProteinModelPortal:   Q31C25
- SMR:   Q31C25
- STRING:   Q31C25
- GeneID:   3765308
- GenomeReviews:   CP000111_GR
- KEGG:   pmi:PMT9312_0509
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- BioCyc:   PMAR74546:PMT9312_0509-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 68131; Mature: 68131

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVTTSFPLGTLRDTPSEAEIISHQLLLKAGYIRRVYSGIYAYMPIMLKVIEKISKIIEI
CEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
ELNSIGCTKLLLPQLHPANLWKKSERWEGYTAGEGIMFNLKDRQGKEFGLAPTHEEVITS
EECCCCCHHHHCCCCCCHHHHCCHHCCCCEECCCCEEEEECCCCCCCCCCCCCHHHHHHH
IASEIINSYKQLPQCFYQIQTKFRDEIRPRFGLMRSREFIMKDGYSFHSSEKDLASFYEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
VGNAYENIFKSCGLETVGVEADSGAIGGASSKEFMVTADAGEDSILFTQSGSYAANIEKA
HHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCHHCCHHH
VSIPSQPIPLKNNISGWIETPEQKTILEVCKNNNLDPSQIIKVVVFLAKFEIKSEVPILA
HCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEE
CIRGDQHINEVKLFNLINKLHSSNLLHLQKIEDIKIIEKNLVNFPLGFIGPDLDNETIKA
EECCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCHHCCCCCCCCEEEC
SSNWDKTWTRIIDHSASTLSKFISGANKVNFHKVFQEFSFTSKDYLIEDIRNAKKGDKIK
CCCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEE
INDNEELKEKKGIEIGHIFQLGQKYSEKLNAKFSDKDGHLKNLWMGCYGIGVTRIAQAAI
ECCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EQNHDQKGICWPIQISPFEVIIIPTNLKDQIQRDLTEQIYNNFLVNKIDVLLDDREDRAG
HCCCCCCCCEEEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
VKFKDAELIGIPFQIIIGRDSINNEVELICRTNNTKFKISAHKLLETFISESEIMYNKNS
CEEECCEEECCCEEEEEECCCCCCCEEEEEECCCCEEEEHHHHHHHHHHCCCCEEECCCC
>Mature Secondary Structure
MRVTTSFPLGTLRDTPSEAEIISHQLLLKAGYIRRVYSGIYAYMPIMLKVIEKISKIIEI
CEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
ELNSIGCTKLLLPQLHPANLWKKSERWEGYTAGEGIMFNLKDRQGKEFGLAPTHEEVITS
EECCCCCHHHHCCCCCCHHHHCCHHCCCCEECCCCEEEEECCCCCCCCCCCCCHHHHHHH
IASEIINSYKQLPQCFYQIQTKFRDEIRPRFGLMRSREFIMKDGYSFHSSEKDLASFYEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
VGNAYENIFKSCGLETVGVEADSGAIGGASSKEFMVTADAGEDSILFTQSGSYAANIEKA
HHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCHHCCHHH
VSIPSQPIPLKNNISGWIETPEQKTILEVCKNNNLDPSQIIKVVVFLAKFEIKSEVPILA
HCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEE
CIRGDQHINEVKLFNLINKLHSSNLLHLQKIEDIKIIEKNLVNFPLGFIGPDLDNETIKA
EECCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCHHCCCCCCCCEEEC
SSNWDKTWTRIIDHSASTLSKFISGANKVNFHKVFQEFSFTSKDYLIEDIRNAKKGDKIK
CCCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEE
INDNEELKEKKGIEIGHIFQLGQKYSEKLNAKFSDKDGHLKNLWMGCYGIGVTRIAQAAI
ECCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EQNHDQKGICWPIQISPFEVIIIPTNLKDQIQRDLTEQIYNNFLVNKIDVLLDDREDRAG
HCCCCCCCCEEEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
VKFKDAELIGIPFQIIIGRDSINNEVELICRTNNTKFKISAHKLLETFISESEIMYNKNS
CEEECCEEECCCEEEEEECCCCCCCEEEEEECCCCEEEEHHHHHHHHHHCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA