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The map label for this gene is leuD [H]

Identifier: 78778642

GI number: 78778642

Start: 245098

End: 245721

Strand: Reverse

Name: leuD [H]

Synonym: PMT9312_0257

Alternate gene names: 78778642

Gene position: 245721-245098 (Counterclockwise)

Preceding gene: 78778643

Following gene: 78778638

Centisome position: 14.38

GC content: 29.65

Gene sequence:

>624_bases
ATGAAATCAGAATTTACCCCACCATTAGGAAAAATTACACAAATAATTGGGGAATGCATCTCATTGATTGGTAACGATAT
TGATACGGATCGAATAATTCCTGCGCGTTTTCTAAAGTGTGTTAATTTTGATTCATTAGGAAAATCTGTATTTGAAGACG
ATAGAGAAACTTTAAAGGGAAAGCATCCTTTCGATTTAGAGAAGCACAAAAATGCTTCAATATTAATTGTTAATAGCAAT
TTTGGATGTGGTTCCAGCAGGGAACATGCCCCCCAAGCACTTATGAGATGGGGTATAAAAGCAATAATAGGTGAGAGTTT
TGCCGAAATTTTTTATAGCAATTGTATTGCAATTGGAATTCCATGTTTCACACTTCCTAAAAAATCAATACAAGATATTC
AAAAATATAACGATAATAAAAGTTTATTTTTAGAAATTGATTTAAAAAATTCATTAGCGAAATCAAAAGATTTAATTTTT
GATCTTGAGATTAAGGAAAGTTCAAGAAAAATGTTTTTATCAGGAGAATGGGATGCAACTTCAACACTACTTGAGAATGA
AAATCTAATTGAAAATAAATTTAATGATTTACCCTATTTGAAATTTAATCCTTATTTCTTGTAG

Upstream 100 bases:

>100_bases
AAGGATCTCCAAGCGGAAGAACACTTCTTATGAGTCCAGCTATGGTTGCTGCCGCCGCAATTAATGGCAAAGTAAGTGAC
GTTAGAGAATTTATAAACAA

Downstream 100 bases:

>100_bases
CTATTTAAATCCAAATAAATTAAAAGAAATTCCTCCAGCTTGAATATGAGGTTGATAAAAAATACCAAATTCATAAGATC
TTTTTTTCCAATTTATAGAG

Product: 3-isopropylmalate dehydratase, small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MKSEFTPPLGKITQIIGECISLIGNDIDTDRIIPARFLKCVNFDSLGKSVFEDDRETLKGKHPFDLEKHKNASILIVNSN
FGCGSSREHAPQALMRWGIKAIIGESFAEIFYSNCIAIGIPCFTLPKKSIQDIQKYNDNKSLFLEIDLKNSLAKSKDLIF
DLEIKESSRKMFLSGEWDATSTLLENENLIENKFNDLPYLKFNPYFL

Sequences:

>Translated_207_residues
MKSEFTPPLGKITQIIGECISLIGNDIDTDRIIPARFLKCVNFDSLGKSVFEDDRETLKGKHPFDLEKHKNASILIVNSN
FGCGSSREHAPQALMRWGIKAIIGESFAEIFYSNCIAIGIPCFTLPKKSIQDIQKYNDNKSLFLEIDLKNSLAKSKDLIF
DLEIKESSRKMFLSGEWDATSTLLENENLIENKFNDLPYLKFNPYFL
>Mature_207_residues
MKSEFTPPLGKITQIIGECISLIGNDIDTDRIIPARFLKCVNFDSLGKSVFEDDRETLKGKHPFDLEKHKNASILIVNSN
FGCGSSREHAPQALMRWGIKAIIGESFAEIFYSNCIAIGIPCFTLPKKSIQDIQKYNDNKSLFLEIDLKNSLAKSKDLIF
DLEIKESSRKMFLSGEWDATSTLLENENLIENKFNDLPYLKFNPYFL

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=187, Percent_Identity=31.0160427807487, Blast_Score=92, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321429, Length=176, Percent_Identity=36.3636363636364, Blast_Score=82, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 23536; Mature: 23536

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSEFTPPLGKITQIIGECISLIGNDIDTDRIIPARFLKCVNFDSLGKSVFEDDRETLKG
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCC
KHPFDLEKHKNASILIVNSNFGCGSSREHAPQALMRWGIKAIIGESFAEIFYSNCIAIGI
CCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCC
PCFTLPKKSIQDIQKYNDNKSLFLEIDLKNSLAKSKDLIFDLEIKESSRKMFLSGEWDAT
CCCCCCHHHHHHHHHCCCCCEEEEEEECCHHHHCCCCEEEEEEECCCCCEEEEECCCCHH
STLLENENLIENKFNDLPYLKFNPYFL
HHHHCCCCHHHHHCCCCCEEECCCCCC
>Mature Secondary Structure
MKSEFTPPLGKITQIIGECISLIGNDIDTDRIIPARFLKCVNFDSLGKSVFEDDRETLKG
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCC
KHPFDLEKHKNASILIVNSNFGCGSSREHAPQALMRWGIKAIIGESFAEIFYSNCIAIGI
CCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCC
PCFTLPKKSIQDIQKYNDNKSLFLEIDLKNSLAKSKDLIFDLEIKESSRKMFLSGEWDAT
CCCCCCHHHHHHHHHCCCCCEEEEEEECCHHHHCCCCEEEEEEECCCCCEEEEECCCCHH
STLLENENLIENKFNDLPYLKFNPYFL
HHHHCCCCHHHHHCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]