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The map label for this gene is leuC

Identifier: 78778643

GI number: 78778643

Start: 245718

End: 247127

Strand: Reverse

Name: leuC

Synonym: PMT9312_0258

Alternate gene names: 78778643

Gene position: 247127-245718 (Counterclockwise)

Preceding gene: 78778644

Following gene: 78778642

Centisome position: 14.46

GC content: 36.88

Gene sequence:

>1410_bases
TTGAGTCAAAATACCTTATTTGATAAAGTTTGGGATTTACACAAAGTTTCTAGTCTACCTGGTGGCTCAGATCAAATTTT
TATTGGTCTTCATCTCATACATGAAGTAACAAGTCCTCAGGCATTTGGTGCTTTAAAAGACAAAAACTTAAAAGTAAAAT
TTCCTAGTAGAACCGTGGCCACTGTTGATCATATTGTGCCAACAGATAATCAAAGCAGACCTTTCAAAGATAATCTTGCA
GAACAAATGATTGAAACACTTGAAAATAACTGCTTACAACATAAAATAAAATTTTTTAATATTGGCAGTGGCAATCAAGG
AATAGTACATGTAGTAGCTCCAGAATTAGGGCTAACTCAGCCAGGAATGACAATCGCTTGTGGTGATTCACACACTTCAA
CTCATGGAGCTTTTGGGTCAATTGCCTTTGGGATAGGCACTAGCCAAGTAAGGGATGTTCTTGCTACCCAAACCATAGCC
ATGAACAAATTGAAGGTGAGACAGATTTGGTGTGAAAATAATTTATCTAATGGCGTTTACGCTAAGGATTTAGTGCTTCA
CATTATTAATGAACTTGGTGTAAAGGCTGGAGTAGGTTTTGCATATGAATTCGCGGGGCCTGCAATCGATGAATTATCAA
TGGAAGAAAGGATGACAATATGCAATATGTCTATTGAAGGGGGCGCGAGATGTGGCTACATAAACCCTGATGAAAAGACC
TTTAGTTACATTAAAAATAGATTATATGCTCCAAAACATACACAATGGGATAAAGCTCTCTTATGGTGGAAATCATTAAA
AAGTGATGAAAATTCAATTTATGATGATGTAATTAAATTAGATGCTTCAAAAGTAGAACCAACCGTAACTTGGGGAATTA
CTCCCGGTCAAAGTTTAGGCATCAATCAACTGATTCCTCTGTTAGATGACTTGCCCCCAAATGACCAATTTATTGCTGAA
GAGGCTTTTGAATATATGGGTTTCATGCCAGGGCAATCAATAAAAGATACTCCAGTAGATGTTTGTTTCATAGGGAGTTG
CACAAATGGGAGAATCAGTGACTTAAGAGTTGCAGCTAAAATATTGAAAGACAAAAAAGTATCTAAAAAAGTAAAAGCAT
TTGTAGTTCCAGGTTCAGAGAAAGTTGCCACTGAAGCCAAAGAAGAAGGTCTTGATAAGATCTTTAAGGATTCTGGATTT
CAATGGAGAGAGCCAGGTTGCTCAATGTGTTTAGCAATGAATTCAGATAAGCTAATAGGTAATCAAGTTAGTGCTAGTTC
TAGCAATAGAAATTTCAAAGGTAGGCAAGGATCTCCAAGCGGAAGAACACTTCTTATGAGTCCAGCTATGGTTGCTGCCG
CCGCAATTAATGGCAAAGTAAGTGACGTTAGAGAATTTATAAACAAATGA

Upstream 100 bases:

>100_bases
AACCCGAAATAGAATAGAAATTCAAACACTAAGTGTAAATGTGTGTTTGAACAGCCTCAGATTAATCCTATTATCGAAGA
GAAAGTAACTTTTTTACAAA

Downstream 100 bases:

>100_bases
AATCAGAATTTACCCCACCATTAGGAAAAATTACACAAATAATTGGGGAATGCATCTCATTGATTGGTAACGATATTGAT
ACGGATCGAATAATTCCTGC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSQNTLFDKVWDLHKVSSLPGGSDQIFIGLHLIHEVTSPQAFGALKDKNLKVKFPSRTVATVDHIVPTDNQSRPFKDNLA
EQMIETLENNCLQHKIKFFNIGSGNQGIVHVVAPELGLTQPGMTIACGDSHTSTHGAFGSIAFGIGTSQVRDVLATQTIA
MNKLKVRQIWCENNLSNGVYAKDLVLHIINELGVKAGVGFAYEFAGPAIDELSMEERMTICNMSIEGGARCGYINPDEKT
FSYIKNRLYAPKHTQWDKALLWWKSLKSDENSIYDDVIKLDASKVEPTVTWGITPGQSLGINQLIPLLDDLPPNDQFIAE
EAFEYMGFMPGQSIKDTPVDVCFIGSCTNGRISDLRVAAKILKDKKVSKKVKAFVVPGSEKVATEAKEEGLDKIFKDSGF
QWREPGCSMCLAMNSDKLIGNQVSASSSNRNFKGRQGSPSGRTLLMSPAMVAAAAINGKVSDVREFINK

Sequences:

>Translated_469_residues
MSQNTLFDKVWDLHKVSSLPGGSDQIFIGLHLIHEVTSPQAFGALKDKNLKVKFPSRTVATVDHIVPTDNQSRPFKDNLA
EQMIETLENNCLQHKIKFFNIGSGNQGIVHVVAPELGLTQPGMTIACGDSHTSTHGAFGSIAFGIGTSQVRDVLATQTIA
MNKLKVRQIWCENNLSNGVYAKDLVLHIINELGVKAGVGFAYEFAGPAIDELSMEERMTICNMSIEGGARCGYINPDEKT
FSYIKNRLYAPKHTQWDKALLWWKSLKSDENSIYDDVIKLDASKVEPTVTWGITPGQSLGINQLIPLLDDLPPNDQFIAE
EAFEYMGFMPGQSIKDTPVDVCFIGSCTNGRISDLRVAAKILKDKKVSKKVKAFVVPGSEKVATEAKEEGLDKIFKDSGF
QWREPGCSMCLAMNSDKLIGNQVSASSSNRNFKGRQGSPSGRTLLMSPAMVAAAAINGKVSDVREFINK
>Mature_468_residues
SQNTLFDKVWDLHKVSSLPGGSDQIFIGLHLIHEVTSPQAFGALKDKNLKVKFPSRTVATVDHIVPTDNQSRPFKDNLAE
QMIETLENNCLQHKIKFFNIGSGNQGIVHVVAPELGLTQPGMTIACGDSHTSTHGAFGSIAFGIGTSQVRDVLATQTIAM
NKLKVRQIWCENNLSNGVYAKDLVLHIINELGVKAGVGFAYEFAGPAIDELSMEERMTICNMSIEGGARCGYINPDEKTF
SYIKNRLYAPKHTQWDKALLWWKSLKSDENSIYDDVIKLDASKVEPTVTWGITPGQSLGINQLIPLLDDLPPNDQFIAEE
AFEYMGFMPGQSIKDTPVDVCFIGSCTNGRISDLRVAAKILKDKKVSKKVKAFVVPGSEKVATEAKEEGLDKIFKDSGFQ
WREPGCSMCLAMNSDKLIGNQVSASSSNRNFKGRQGSPSGRTLLMSPAMVAAAAINGKVSDVREFINK

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=426, Percent_Identity=26.2910798122066, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI41352693, Length=372, Percent_Identity=25.2688172043011, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1786259, Length=461, Percent_Identity=49.0238611713666, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1787531, Length=366, Percent_Identity=26.775956284153, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87081781, Length=361, Percent_Identity=22.7146814404432, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI2367097, Length=109, Percent_Identity=32.1100917431193, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI32564738, Length=394, Percent_Identity=28.1725888324873, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI25149337, Length=394, Percent_Identity=28.1725888324873, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI25149342, Length=320, Percent_Identity=28.75, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17568399, Length=401, Percent_Identity=26.9326683291771, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=50.9554140127389, Blast_Score=443, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6322261, Length=410, Percent_Identity=26.3414634146341, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6320440, Length=455, Percent_Identity=28.1318681318681, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6323335, Length=393, Percent_Identity=26.4631043256997, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI281365315, Length=392, Percent_Identity=27.2959183673469, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17864292, Length=392, Percent_Identity=27.2959183673469, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161076999, Length=392, Percent_Identity=27.2959183673469, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28571643, Length=420, Percent_Identity=25.952380952381, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24645686, Length=460, Percent_Identity=28.4782608695652, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI17137564, Length=373, Percent_Identity=28.4182305630027, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_PROM9 (Q31CS6)

Other databases:

- EMBL:   CP000111
- RefSeq:   YP_396755.1
- ProteinModelPortal:   Q31CS6
- SMR:   Q31CS6
- STRING:   Q31CS6
- GeneID:   3765044
- GenomeReviews:   CP000111_GR
- KEGG:   pmi:PMT9312_0258
- eggNOG:   COG0065
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- ProtClustDB:   PRK05478
- BioCyc:   PMAR74546:PMT9312_0258-MONOMER
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 51405; Mature: 51274

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNTLFDKVWDLHKVSSLPGGSDQIFIGLHLIHEVTSPQAFGALKDKNLKVKFPSRTVA
CCCCHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHCCCHHHCCCCCCCEEEECCCCCCH
TVDHIVPTDNQSRPFKDNLAEQMIETLENNCLQHKIKFFNIGSGNQGIVHVVAPELGLTQ
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCEEEEEECCCCCCC
PGMTIACGDSHTSTHGAFGSIAFGIGTSQVRDVLATQTIAMNKLKVRQIWCENNLSNGVY
CCCEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCH
AKDLVLHIINELGVKAGVGFAYEFAGPAIDELSMEERMTICNMSIEGGARCGYINPDEKT
HHHHHHHHHHHHCHHHCCCEEEECCCCCHHHHCHHHHHEEEEEEECCCCCCCCCCCCHHH
FSYIKNRLYAPKHTQWDKALLWWKSLKSDENSIYDDVIKLDASKVEPTVTWGITPGQSLG
HHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCC
INQLIPLLDDLPPNDQFIAEEAFEYMGFMPGQSIKDTPVDVCFIGSCTNGRISDLRVAAK
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHH
ILKDKKVSKKVKAFVVPGSEKVATEAKEEGLDKIFKDSGFQWREPGCSMCLAMNSDKLIG
HHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCEEEEECCCCCCC
NQVSASSSNRNFKGRQGSPSGRTLLMSPAMVAAAAINGKVSDVREFINK
CCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure 
SQNTLFDKVWDLHKVSSLPGGSDQIFIGLHLIHEVTSPQAFGALKDKNLKVKFPSRTVA
CCCHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHCCCHHHCCCCCCCEEEECCCCCCH
TVDHIVPTDNQSRPFKDNLAEQMIETLENNCLQHKIKFFNIGSGNQGIVHVVAPELGLTQ
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCEEEEEECCCCCCC
PGMTIACGDSHTSTHGAFGSIAFGIGTSQVRDVLATQTIAMNKLKVRQIWCENNLSNGVY
CCCEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCH
AKDLVLHIINELGVKAGVGFAYEFAGPAIDELSMEERMTICNMSIEGGARCGYINPDEKT
HHHHHHHHHHHHCHHHCCCEEEECCCCCHHHHCHHHHHEEEEEEECCCCCCCCCCCCHHH
FSYIKNRLYAPKHTQWDKALLWWKSLKSDENSIYDDVIKLDASKVEPTVTWGITPGQSLG
HHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCC
INQLIPLLDDLPPNDQFIAEEAFEYMGFMPGQSIKDTPVDVCFIGSCTNGRISDLRVAAK
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHH
ILKDKKVSKKVKAFVVPGSEKVATEAKEEGLDKIFKDSGFQWREPGCSMCLAMNSDKLIG
HHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEECCCCCCEEEEECCCCCCC
NQVSASSSNRNFKGRQGSPSGRTLLMSPAMVAAAAINGKVSDVREFINK
CCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA