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The map label for this gene is def

Identifier: 78778455

GI number: 78778455

Start: 73488

End: 74093

Strand: Reverse

Name: def

Synonym: PMT9312_0070

Alternate gene names: 78778455

Gene position: 74093-73488 (Counterclockwise)

Preceding gene: 78778457

Following gene: 78778454

Centisome position: 4.33

GC content: 32.67

Gene sequence:

>606_bases
GTGGCAAACCATTTTTCACAACTTGCAAAAAAGTCAAAAACAAATGGGAACTCGGAAAAAATTGCAAAAGAACAAACAGG
TAAGCCATCTCTCGAAATTTATAAACTTGGTGATGATGTTTTAAGACAAAATTCCAAAAGAATAACTAAGGTTGATGAAT
CGATTAGAAAACTTACTAGAGAAATGATTCAAAGCATGTATGCGGCCAAAGGAATTGGACTTGCTGCACCTCAAATTGGT
ATTAATAAAGAGCTTCTTGTGATTGATGTAAATTTTGAAGATTCAGCTGCAGAACCTTTAATCTTAATCAATCCAGAAAT
TACAGACTATGGAACAACCCTGAATTCATACGAAGAAGGCTGCTTGAGCATACCTGGTGTATATTTAAATGTAGTAAGAC
CATCAACTATAAAATTAAAATTTAGAGATGAAATGGGAAGACCACGTAAAATGAAAGCAGATGGACTTTTAGCTAGGTGT
ATTCAACACGAAATGGATCACTTAAATGGAATATTATTTGTTGATAGAGTTACATCAAAGGATGACTTAAAAAAAGAACT
TATAAAAGAAGGGTTTCACGAAAAAGACGTTATTTCGATAACTTAA

Upstream 100 bases:

>100_bases
ATTTATCATCATTACTCATTTATAAAACTATAAAGAAAGTAAATTAAAGTGCTAGTATTTTTATAGCTAAAGTCTTCATT
AAAAACTTTTTTTTATTAAC

Downstream 100 bases:

>100_bases
TTTAATGACTGAAACTACAATATTTCAAAAAATTCTTAATGAAGAAATCCCCTGCGATAAGCTTTATGAAGATGAGTTTT
GTATTGCATTTAATGATATC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MANHFSQLAKKSKTNGNSEKIAKEQTGKPSLEIYKLGDDVLRQNSKRITKVDESIRKLTREMIQSMYAAKGIGLAAPQIG
INKELLVIDVNFEDSAAEPLILINPEITDYGTTLNSYEEGCLSIPGVYLNVVRPSTIKLKFRDEMGRPRKMKADGLLARC
IQHEMDHLNGILFVDRVTSKDDLKKELIKEGFHEKDVISIT

Sequences:

>Translated_201_residues
MANHFSQLAKKSKTNGNSEKIAKEQTGKPSLEIYKLGDDVLRQNSKRITKVDESIRKLTREMIQSMYAAKGIGLAAPQIG
INKELLVIDVNFEDSAAEPLILINPEITDYGTTLNSYEEGCLSIPGVYLNVVRPSTIKLKFRDEMGRPRKMKADGLLARC
IQHEMDHLNGILFVDRVTSKDDLKKELIKEGFHEKDVISIT
>Mature_200_residues
ANHFSQLAKKSKTNGNSEKIAKEQTGKPSLEIYKLGDDVLRQNSKRITKVDESIRKLTREMIQSMYAAKGIGLAAPQIGI
NKELLVIDVNFEDSAAEPLILINPEITDYGTTLNSYEEGCLSIPGVYLNVVRPSTIKLKFRDEMGRPRKMKADGLLARCI
QHEMDHLNGILFVDRVTSKDDLKKELIKEGFHEKDVISIT

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=170, Percent_Identity=28.2352941176471, Blast_Score=86, Evalue=2e-17,
Organism=Escherichia coli, GI1789682, Length=167, Percent_Identity=41.3173652694611, Blast_Score=120, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24645728, Length=169, Percent_Identity=29.585798816568, Blast_Score=87, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24645726, Length=174, Percent_Identity=29.8850574712644, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_PROM9 (Q31DB4)

Other databases:

- EMBL:   CP000111
- RefSeq:   YP_396567.1
- HSSP:   P0A6K3
- ProteinModelPortal:   Q31DB4
- SMR:   Q31DB4
- STRING:   Q31DB4
- GeneID:   3764853
- GenomeReviews:   CP000111_GR
- KEGG:   pmi:PMT9312_0070
- eggNOG:   COG0242
- HOGENOM:   HBG665227
- OMA:   RITKVDE
- ProtClustDB:   PRK00150
- BioCyc:   PMAR74546:PMT9312_0070-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 22588; Mature: 22457

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: NA

Important sites: ACT_SITE 164-164

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANHFSQLAKKSKTNGNSEKIAKEQTGKPSLEIYKLGDDVLRQNSKRITKVDESIRKLTR
CCHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEECCHHHHHCCCHHHHHHHHHHHHHHH
EMIQSMYAAKGIGLAAPQIGINKELLVIDVNFEDSAAEPLILINPEITDYGTTLNSYEEG
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEECCCCCCCCCCHHHHHCC
CLSIPGVYLNVVRPSTIKLKFRDEMGRPRKMKADGLLARCIQHEMDHLNGILFVDRVTSK
CCCCCCEEEEEECCCEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCH
DDLKKELIKEGFHEKDVISIT
HHHHHHHHHCCCCCCCEEEEC
>Mature Secondary Structure 
ANHFSQLAKKSKTNGNSEKIAKEQTGKPSLEIYKLGDDVLRQNSKRITKVDESIRKLTR
CHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEECCHHHHHCCCHHHHHHHHHHHHHHH
EMIQSMYAAKGIGLAAPQIGINKELLVIDVNFEDSAAEPLILINPEITDYGTTLNSYEEG
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEECCCCCCCCCCHHHHHCC
CLSIPGVYLNVVRPSTIKLKFRDEMGRPRKMKADGLLARCIQHEMDHLNGILFVDRVTSK
CCCCCCEEEEEECCCEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCH
DDLKKELIKEGFHEKDVISIT
HHHHHHHHHCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA