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The map label for this gene is gatB

Identifier: 78778434

GI number: 78778434

Start: 52423

End: 53895

Strand: Direct

Name: gatB

Synonym: PMT9312_0049

Alternate gene names: 78778434

Gene position: 52423-53895 (Clockwise)

Preceding gene: 78778432

Following gene: 78778436

Centisome position: 3.07

GC content: 31.7

Gene sequence:

>1473_bases
ATGAACAATTTGGAATCTTGGGAAGCTGTGATTGGTTTGGAAACTCATGTACAGCTTAATACGAAAAGTAAAATATTCAC
ATCTGCGTCAACAGCTTTTGGTGATGCACCTAATACGCATATAGATCCTGTAGTTTGTGGGTTACCAGGAACTCTTCCAG
TTTTGAATGAGACTGTTCTTGAGTATGCTGTAAAAACTTCGTTAGCATTAAATTTAAACGTTGCAGAACATTGTAAATTT
GATAGAAAACAATATTTTTATCCTGATCTGCCTAAAAATTATCAAATTTCACAATTTGATGAGCCACTAGCTGAAAATGG
CTGGTTAGAGGTTGAAATAATAGAAAAGGATAAAGATCCTTATACAAAAAAAATTGGTATAGAAAGGTTACATATGGAAG
AAGATGCTGGTAAATTAGTTCATTCAGGCAGTGACAGATTAGCTGGTTCTAAGTATTCCTTAGTTGATTACAATCGTGCT
GGAATAGCACTTTGTGAAATTGTAAGTAAACCAGACATTAGAACAGGTAGAGAGGCAGCTGAATATGCTTCTGAGATTAG
AAGAACAGTTAGATATCTAGGTGTATCAGATGGAAATATGCAGGAGGGTTCATTAAGATGCGATGTTAATATTTCAGTTA
GAAAAGGACCTAATGCTCCTTTTGGTACCAAAGTGGAAATTAAGAATATGAATTCATTTTCTGCAATTCAAAAGGCTTGT
GATTATGAAATAGCAAGACAAATAGAAGTTTATGAAAATGGAGGAGAAATCTTCCAAGAAACAAGGTTATGGGATGAAGC
TAAGCAATTAACAAAAAGTATGAGATTAAAAGAAGGTAGTAGTGACTATAGATATTTTCCTGATCCTGACTTAGGACCAA
TTGAAATTACAAAAGCACAAAAAGAAATATGGTTTAATGAACTACCAGAATTACCTTCAAAGAAAAGAAATAAATATGTG
AATGAATTTGGTTTGTCTGCATATGATGCACGGGTAATTTCTGATGAAATTAACATGGCAAATTTTTTTGAAGAAACTGT
AGCTAATGGTGCTGAGGCTAAACTAGCTTCAAATTGGGTGACAAGTGATATCGTGGGCTATTTAAAAGCAAATAAACTTA
GTTTTAGTGAATTAAAGCTTAGTCCTGAAAATCTTGCTGAAATGATTAATATGATTTTAAAAAATACTATTAGTGGAAAA
ATTGCAAAAGAAATTTTACCTGAATTAATTAAAAAAAATATTTCTCCGAAAAAATTAGTTGAAGAAAAAGGGTTGGCTAT
GATATCTGATTCTTCAAGTATTTTGCCAATAATTAATGAACTTATAAATGAATATCCAAATGAAGTTCAAGCATTTAGAA
ATGGTAAAACTAAATTACTTGGTTTTTTTATTGGTCAACTTATGAAAAGAACTAAAGGTAAAGCTGACCCTAAACTTGCA
AATAAACTTCTTGCAGAAAAGTTGAATGGCTAA

Upstream 100 bases:

>100_bases
ATTTTTGGTTTCTTGTGCCATTTAAAAATTAATATTTTTATTTGTGTTTTGGTCCTCTTTTCCTTTATATCCAATAATAT
TAATAAAGAGACTTTTGATA

Downstream 100 bases:

>100_bases
AGCTTCAAAGAATTTCTCTAATCGTGTTGATCCATTTATTTTGGTCATCTGAATTATCTAAAATAATATCTGAAAATTTA
CTTTTTTCTTCAAAACTTAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MNNLESWEAVIGLETHVQLNTKSKIFTSASTAFGDAPNTHIDPVVCGLPGTLPVLNETVLEYAVKTSLALNLNVAEHCKF
DRKQYFYPDLPKNYQISQFDEPLAENGWLEVEIIEKDKDPYTKKIGIERLHMEEDAGKLVHSGSDRLAGSKYSLVDYNRA
GIALCEIVSKPDIRTGREAAEYASEIRRTVRYLGVSDGNMQEGSLRCDVNISVRKGPNAPFGTKVEIKNMNSFSAIQKAC
DYEIARQIEVYENGGEIFQETRLWDEAKQLTKSMRLKEGSSDYRYFPDPDLGPIEITKAQKEIWFNELPELPSKKRNKYV
NEFGLSAYDARVISDEINMANFFEETVANGAEAKLASNWVTSDIVGYLKANKLSFSELKLSPENLAEMINMILKNTISGK
IAKEILPELIKKNISPKKLVEEKGLAMISDSSSILPIINELINEYPNEVQAFRNGKTKLLGFFIGQLMKRTKGKADPKLA
NKLLAEKLNG

Sequences:

>Translated_490_residues
MNNLESWEAVIGLETHVQLNTKSKIFTSASTAFGDAPNTHIDPVVCGLPGTLPVLNETVLEYAVKTSLALNLNVAEHCKF
DRKQYFYPDLPKNYQISQFDEPLAENGWLEVEIIEKDKDPYTKKIGIERLHMEEDAGKLVHSGSDRLAGSKYSLVDYNRA
GIALCEIVSKPDIRTGREAAEYASEIRRTVRYLGVSDGNMQEGSLRCDVNISVRKGPNAPFGTKVEIKNMNSFSAIQKAC
DYEIARQIEVYENGGEIFQETRLWDEAKQLTKSMRLKEGSSDYRYFPDPDLGPIEITKAQKEIWFNELPELPSKKRNKYV
NEFGLSAYDARVISDEINMANFFEETVANGAEAKLASNWVTSDIVGYLKANKLSFSELKLSPENLAEMINMILKNTISGK
IAKEILPELIKKNISPKKLVEEKGLAMISDSSSILPIINELINEYPNEVQAFRNGKTKLLGFFIGQLMKRTKGKADPKLA
NKLLAEKLNG
>Mature_490_residues
MNNLESWEAVIGLETHVQLNTKSKIFTSASTAFGDAPNTHIDPVVCGLPGTLPVLNETVLEYAVKTSLALNLNVAEHCKF
DRKQYFYPDLPKNYQISQFDEPLAENGWLEVEIIEKDKDPYTKKIGIERLHMEEDAGKLVHSGSDRLAGSKYSLVDYNRA
GIALCEIVSKPDIRTGREAAEYASEIRRTVRYLGVSDGNMQEGSLRCDVNISVRKGPNAPFGTKVEIKNMNSFSAIQKAC
DYEIARQIEVYENGGEIFQETRLWDEAKQLTKSMRLKEGSSDYRYFPDPDLGPIEITKAQKEIWFNELPELPSKKRNKYV
NEFGLSAYDARVISDEINMANFFEETVANGAEAKLASNWVTSDIVGYLKANKLSFSELKLSPENLAEMINMILKNTISGK
IAKEILPELIKKNISPKKLVEEKGLAMISDSSSILPIINELINEYPNEVQAFRNGKTKLLGFFIGQLMKRTKGKADPKLA
NKLLAEKLNG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=500, Percent_Identity=37.8, Blast_Score=327, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI115534094, Length=352, Percent_Identity=37.7840909090909, Blast_Score=219, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6319391, Length=518, Percent_Identity=31.4671814671815, Blast_Score=227, Evalue=4e-60,
Organism=Drosophila melanogaster, GI45550808, Length=496, Percent_Identity=34.2741935483871, Blast_Score=273, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_PROM9 (Q31DD5)

Other databases:

- EMBL:   CP000111
- RefSeq:   YP_396546.1
- ProteinModelPortal:   Q31DD5
- SMR:   Q31DD5
- STRING:   Q31DD5
- GeneID:   3764830
- GenomeReviews:   CP000111_GR
- KEGG:   pmi:PMT9312_0049
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   PMAR74546:PMT9312_0049-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 54980; Mature: 54980

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLESWEAVIGLETHVQLNTKSKIFTSASTAFGDAPNTHIDPVVCGLPGTLPVLNETVL
CCCHHHHHHHEEEEEEEEECCCCCEEECCCHHCCCCCCCCCCEEEECCCCCCHHHHHHHH
EYAVKTSLALNLNVAEHCKFDRKQYFYPDLPKNYQISQFDEPLAENGWLEVEIIEKDKDP
HHHHHHHHEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCEEEEEEECCCCC
YTKKIGIERLHMEEDAGKLVHSGSDRLAGSKYSLVDYNRAGIALCEIVSKPDIRTGREAA
CHHHHCHHHHHCHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHCCCCCCCCHHHH
EYASEIRRTVRYLGVSDGNMQEGSLRCDVNISVRKGPNAPFGTKVEIKNMNSFSAIQKAC
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHH
DYEIARQIEVYENGGEIFQETRLWDEAKQLTKSMRLKEGSSDYRYFPDPDLGPIEITKAQ
HHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHH
KEIWFNELPELPSKKRNKYVNEFGLSAYDARVISDEINMANFFEETVANGAEAKLASNWV
HHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
TSDIVGYLKANKLSFSELKLSPENLAEMINMILKNTISGKIAKEILPELIKKNISPKKLV
HHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
EEKGLAMISDSSSILPIINELINEYPNEVQAFRNGKTKLLGFFIGQLMKRTKGKADPKLA
HHCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHH
NKLLAEKLNG
HHHHHHHCCC
>Mature Secondary Structure
MNNLESWEAVIGLETHVQLNTKSKIFTSASTAFGDAPNTHIDPVVCGLPGTLPVLNETVL
CCCHHHHHHHEEEEEEEEECCCCCEEECCCHHCCCCCCCCCCEEEECCCCCCHHHHHHHH
EYAVKTSLALNLNVAEHCKFDRKQYFYPDLPKNYQISQFDEPLAENGWLEVEIIEKDKDP
HHHHHHHHEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCEEEEEEECCCCC
YTKKIGIERLHMEEDAGKLVHSGSDRLAGSKYSLVDYNRAGIALCEIVSKPDIRTGREAA
CHHHHCHHHHHCHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHCCCCCCCCHHHH
EYASEIRRTVRYLGVSDGNMQEGSLRCDVNISVRKGPNAPFGTKVEIKNMNSFSAIQKAC
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHH
DYEIARQIEVYENGGEIFQETRLWDEAKQLTKSMRLKEGSSDYRYFPDPDLGPIEITKAQ
HHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHH
KEIWFNELPELPSKKRNKYVNEFGLSAYDARVISDEINMANFFEETVANGAEAKLASNWV
HHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
TSDIVGYLKANKLSFSELKLSPENLAEMINMILKNTISGKIAKEILPELIKKNISPKKLV
HHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
EEKGLAMISDSSSILPIINELINEYPNEVQAFRNGKTKLLGFFIGQLMKRTKGKADPKLA
HHCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHH
NKLLAEKLNG
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA