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The map label for this gene is 78778420

Identifier: 78778420

GI number: 78778420

Start: 32419

End: 33582

Strand: Reverse

Name: 78778420

Synonym: PMT9312_0035

Alternate gene names: NA

Gene position: 33582-32419 (Counterclockwise)

Preceding gene: 78778423

Following gene: 78778418

Centisome position: 1.96

GC content: 33.59

Gene sequence:

>1164_bases
GTGGCCATACAACAAAAATTATCATTGATGATTCCTGGACCCACCCCAGTTCCAGAAAAAGTTTTACAAGCATTAAGTAA
GCATCCGATAGGACATCGCAGTAAAGAATTCCAAGATCTCGTAGAAAGTACTACTAAAAATTTACAGTGGCTTCATCAAA
CTCAAAATGATGTTCTAACAATTACTGGTAGTGGAACTGCCGCAATGGAAGCTGGAATAATAAATACCTTAAGTAAAGGA
GATAAAGTAATTTGTGGAGAAAATGGAAAATTCGGCGAAAGATGGGTAAAAGTTGCTAAAGAATTTGGTTTAGAAGTAAT
AAAAATTGATTCCGAATGGGGTACTCCGCTTGATCCAGAAGAATTCAAAAAGGTATTAGAGGAAGATAACCAAAAAGAAA
TAAAGGGAGTTATTTTGACTCATTCTGAAACCTCAACAGGTGTAATTAATGATCTAGAAACTATAAGTTCATATATTCGC
GAACACAATACAGCTTTATCAATTGTTGACTGCGTTACAAGTCTTGGAGCTTGCAATGTACCAGTAGATGAATGGAAATT
AGATATCGTTGCGTCAGGATCACAAAAGGGATATATGATACCTCCAGGGCTTAGTTTTATAGCAATGAGCCAAAAAGCAT
GGGAAGCTGCAGAAAAATCTAATTTACCAAAATTTTATTTAAATTTAAAATCCTACAGAAAGAGTCTTTTAAGTAACAGT
AATCCATATACTCCAGCAGTTAATTTGGTTTTTGCTTTAGATGAAGCTTTAACAATGATGAGAGAAGAAGGCTTAGATAA
CATTTTCAACAGACACAATAAACATAAATTAGCAATGAGCAATGCTGTAAAGGCTTTAAATCTAAAATTATTTGCTGATG
AAAAATATTTAAGCCCTTCAATTACTGCAATAGAAACTGGAGAAATGGATGCTGAAGAATTCAGAAAAACAATAAAGAAT
AATTTTGATATTTTACTTGCTGGTGGTCAAGATCATTTAAAGGGGAAAATATTTAGAGTCGGCCACTTAGGTTATGTAAA
CGATCGAGATATTATTACAGTAGTTTCTGCTATAAGTAATACACTTCTTGAGCTCGGTAAAATTACTGCCCAACAAGCTG
GTGAAGCATTAGTTGTAGTATCTAAGTATCTTGATGGAAATTAA

Upstream 100 bases:

>100_bases
CTCCAGCAACCCACAAAGGTAAAGAAAATCCTTTTTTCAAATCAAGTAATTGAAAAATATATAATGAGAACTAATCTAAG
AATGACCTATTTAACAAAAA

Downstream 100 bases:

>100_bases
GTTAAGTACCTGGCTCTTCAACATTTCCGCCTAATTCAACAATCTTTTTTCTGAGAATGATTGATTTTTTTTCATCCCCC
TTCGTTTGAGCGATTGCAAG

Product: soluble hydrogenase small subunit

Products: NA

Alternate protein names: Tritium exchange subunit [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MAIQQKLSLMIPGPTPVPEKVLQALSKHPIGHRSKEFQDLVESTTKNLQWLHQTQNDVLTITGSGTAAMEAGIINTLSKG
DKVICGENGKFGERWVKVAKEFGLEVIKIDSEWGTPLDPEEFKKVLEEDNQKEIKGVILTHSETSTGVINDLETISSYIR
EHNTALSIVDCVTSLGACNVPVDEWKLDIVASGSQKGYMIPPGLSFIAMSQKAWEAAEKSNLPKFYLNLKSYRKSLLSNS
NPYTPAVNLVFALDEALTMMREEGLDNIFNRHNKHKLAMSNAVKALNLKLFADEKYLSPSITAIETGEMDAEEFRKTIKN
NFDILLAGGQDHLKGKIFRVGHLGYVNDRDIITVVSAISNTLLELGKITAQQAGEALVVVSKYLDGN

Sequences:

>Translated_387_residues
MAIQQKLSLMIPGPTPVPEKVLQALSKHPIGHRSKEFQDLVESTTKNLQWLHQTQNDVLTITGSGTAAMEAGIINTLSKG
DKVICGENGKFGERWVKVAKEFGLEVIKIDSEWGTPLDPEEFKKVLEEDNQKEIKGVILTHSETSTGVINDLETISSYIR
EHNTALSIVDCVTSLGACNVPVDEWKLDIVASGSQKGYMIPPGLSFIAMSQKAWEAAEKSNLPKFYLNLKSYRKSLLSNS
NPYTPAVNLVFALDEALTMMREEGLDNIFNRHNKHKLAMSNAVKALNLKLFADEKYLSPSITAIETGEMDAEEFRKTIKN
NFDILLAGGQDHLKGKIFRVGHLGYVNDRDIITVVSAISNTLLELGKITAQQAGEALVVVSKYLDGN
>Mature_386_residues
AIQQKLSLMIPGPTPVPEKVLQALSKHPIGHRSKEFQDLVESTTKNLQWLHQTQNDVLTITGSGTAAMEAGIINTLSKGD
KVICGENGKFGERWVKVAKEFGLEVIKIDSEWGTPLDPEEFKKVLEEDNQKEIKGVILTHSETSTGVINDLETISSYIRE
HNTALSIVDCVTSLGACNVPVDEWKLDIVASGSQKGYMIPPGLSFIAMSQKAWEAAEKSNLPKFYLNLKSYRKSLLSNSN
PYTPAVNLVFALDEALTMMREEGLDNIFNRHNKHKLAMSNAVKALNLKLFADEKYLSPSITAIETGEMDAEEFRKTIKNN
FDILLAGGQDHLKGKIFRVGHLGYVNDRDIITVVSAISNTLLELGKITAQQAGEALVVVSKYLDGN

Specific function: Soluble hydrogenase catalyzes both production and consumption of hydrogen from suitable artificial electron donors or acceptors. This subunit catalyzes the tritium-exchange activity [H]

COG id: COG0075

COG function: function code E; Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557289, Length=357, Percent_Identity=30.2521008403361, Blast_Score=162, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17536281, Length=379, Percent_Identity=30.8707124010554, Blast_Score=152, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6321079, Length=364, Percent_Identity=25.5494505494505, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI17530823, Length=371, Percent_Identity=26.9541778975741, Blast_Score=142, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: 2.6.1.-

Molecular weight: Translated: 42699; Mature: 42568

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIQQKLSLMIPGPTPVPEKVLQALSKHPIGHRSKEFQDLVESTTKNLQWLHQTQNDVLT
CCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEE
ITGSGTAAMEAGIINTLSKGDKVICGENGKFGERWVKVAKEFGLEVIKIDSEWGTPLDPE
EECCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCHH
EFKKVLEEDNQKEIKGVILTHSETSTGVINDLETISSYIREHNTALSIVDCVTSLGACNV
HHHHHHHCCCCHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCC
PVDEWKLDIVASGSQKGYMIPPGLSFIAMSQKAWEAAEKSNLPKFYLNLKSYRKSLLSNS
CHHHEEEEEEECCCCCCEECCCCCHHEEHHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCC
NPYTPAVNLVFALDEALTMMREEGLDNIFNRHNKHKLAMSNAVKALNLKLFADEKYLSPS
CCCCHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHEEEEEECCCCCCCC
ITAIETGEMDAEEFRKTIKNNFDILLAGGQDHLKGKIFRVGHLGYVNDRDIITVVSAISN
CEEEECCCCCHHHHHHHHHCCCEEEEECCCHHHCCEEEEEEECCCCCCCHHHHHHHHHHH
TLLELGKITAQQAGEALVVVSKYLDGN
HHHHHHHHHHHHCCCEEEEEEHHCCCC
>Mature Secondary Structure 
AIQQKLSLMIPGPTPVPEKVLQALSKHPIGHRSKEFQDLVESTTKNLQWLHQTQNDVLT
CCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEE
ITGSGTAAMEAGIINTLSKGDKVICGENGKFGERWVKVAKEFGLEVIKIDSEWGTPLDPE
EECCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCHH
EFKKVLEEDNQKEIKGVILTHSETSTGVINDLETISSYIREHNTALSIVDCVTSLGACNV
HHHHHHHCCCCHHCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCC
PVDEWKLDIVASGSQKGYMIPPGLSFIAMSQKAWEAAEKSNLPKFYLNLKSYRKSLLSNS
CHHHEEEEEEECCCCCCEECCCCCHHEEHHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCC
NPYTPAVNLVFALDEALTMMREEGLDNIFNRHNKHKLAMSNAVKALNLKLFADEKYLSPS
CCCCHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHEEEEEECCCCCCCC
ITAIETGEMDAEEFRKTIKNNFDILLAGGQDHLKGKIFRVGHLGYVNDRDIITVVSAISN
CEEEECCCCCHHHHHHHHHCCCEEEEECCCHHHCCEEEEEEECCCCCCCHHHHHHHHHHH
TLLELGKITAQQAGEALVVVSKYLDGN
HHHHHHHHHHHHCCCEEEEEEHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2129525 [H]