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The map label for this gene is adhE [H]

Identifier: 78358319

GI number: 78358319

Start: 3268562

End: 3270022

Strand: Reverse

Name: adhE [H]

Synonym: Dde_3279

Alternate gene names: 78358319

Gene position: 3270022-3268562 (Counterclockwise)

Preceding gene: 78358320

Following gene: 78358318

Centisome position: 87.66

GC content: 59.69

Gene sequence:

>1461_bases
ATGGTAGACAAGGATTTATTGTCCATTCAGGAAGCCCGTGCGCTGGTGCGCGCCGCCCGTGTTGCTCAGGCTGAGTACGC
TCAGCTGGGTCAGGAGCGCGTGGACGCCATCGTCAAGGCGATTGCCGATGCCGCCGCCGCTCAGGCAGAGCAGCTCGCCG
CACTGGCAGTGGAAGAAACCGGCTTCGGCAAGGTGCAGGATAAAAAGCAGAAGAATATCCTCGCCAGTGAAAAGCTGATC
GAAGCCATCAAGGATATGAAAACCATCGGCGTGCTGAACGACGACAAGGCCAGAAAGGTCGTCGAGATAGCCGTACCCAT
GGGGGTCATCGCAGGGATTGTTCCCTCCACCAACCCCACGTCTACGACCATCTACAAGTCCATTATTTCGCTGAAGTCCG
GCAACGCCATTGTGTTTACGCCGCACCCCAGCGCAAAGAAGTGCATCGGCAGAACCGTGGAAATCATCCGCCGCGTGCTG
CATGACTGCGGCGTGAGCGAAGATCTGGTGAGCGTCATGAGCGTGCCCACCATTGAAGGCAGCGGCGAGCTGATGCGTCA
GGCTGATCTTATTCTGGCCACCGGCGGTCCCGGCATGGTCAAGGCCGCATACAGCTCCGGCACGCCCGCTCTGGGTGTGG
GCGCAGGCAACGTGCCCGCCTTCATCGAGCGTACCGCCGACATCGAAGACGCCGTGGCAAAGATTTTTGCCAGCAAGACG
TTTGACAACGGCACCATCTGCGCTTCTGAGCAGTCCATTGTCACCGAAGCCGTCATTGCCGACAAAGTGCGCGCAGCGCT
GGTCAGAAACGGCGGTTACTTCCTCGAAGGTGAAAAGCTGGAAAAGGTCAAGCGCGTCATGGAGCGCGGCAACGGCTCCA
TGAACCCCGCCATAGTGGGCCGCGACGCCCTGCACATTGCCAAACTGGCAGGCATCGAAGTTCCCGCAGGTACCCGCCTG
CTTATTTCCGATGAGCCCGGCGTGGGACCCCGTTACCCCTTCTCCAAGGAAAAACTGACAGCTCTTCTCGGCTTTTATGT
TGTGGAAGACTGGAAGGAAGCCTGCGAAATGTGCCACGCCCTGCTGAAAAACGGCGGCATCGGCCATTCGCTTGCCATCC
ATTCGCGCAATGAAGAAGTCATCCGTGAATTCGGCATGAAGAAGCCCGTTTCGCGCATGCTGGTAAATACGCCCTCAACG
CAGGGTGCCGTGGGCCTTTCCACCTCGCTGTTCCCCTCGTTCACGCTGGGTTGCGGCGCCGTGGGCGGCAGCTCCACTTC
CGACAACGTCACGCCGCTGAACCTGATGAACGTGCGCCGCATCGCGTACGATCTGGGCACCGTGTGCTGCCAGCCCGCCG
CACCGGCAGCCTGCGCGCAGCCTGCATCGTCTGCCTCGTCCTCCATTGACGTGCAGGCCATCACGCAGCTGATCGTGGAA
CAACTGAAAAAAATGGCCTAA

Upstream 100 bases:

>100_bases
AGCGGTCTTTCCATACCCCGCGAGGCGGTCAAATCTGCGAACAAAAAAGATCTGATTGACGCGATCATCAATTCATACAG
GCAGATAGAGGAGTAGTCCC

Downstream 100 bases:

>100_bases
GCCCCGGCTGTGGCTTGTAACAATATATAAAAACAGACTTTTAAGGAGAATAGCATGTCCTCTTCCAACGCACTCGGTAT
GATTGAAACCAGAGGTCTTG

Product: aldehyde dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase; ADH; Acetaldehyde dehydrogenase [acetylating]; ACDH; Pyruvate-formate-lyase deactivase; PFL deactivase [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MVDKDLLSIQEARALVRAARVAQAEYAQLGQERVDAIVKAIADAAAAQAEQLAALAVEETGFGKVQDKKQKNILASEKLI
EAIKDMKTIGVLNDDKARKVVEIAVPMGVIAGIVPSTNPTSTTIYKSIISLKSGNAIVFTPHPSAKKCIGRTVEIIRRVL
HDCGVSEDLVSVMSVPTIEGSGELMRQADLILATGGPGMVKAAYSSGTPALGVGAGNVPAFIERTADIEDAVAKIFASKT
FDNGTICASEQSIVTEAVIADKVRAALVRNGGYFLEGEKLEKVKRVMERGNGSMNPAIVGRDALHIAKLAGIEVPAGTRL
LISDEPGVGPRYPFSKEKLTALLGFYVVEDWKEACEMCHALLKNGGIGHSLAIHSRNEEVIREFGMKKPVSRMLVNTPST
QGAVGLSTSLFPSFTLGCGAVGGSSTSDNVTPLNLMNVRRIAYDLGTVCCQPAAPAACAQPASSASSSIDVQAITQLIVE
QLKKMA

Sequences:

>Translated_486_residues
MVDKDLLSIQEARALVRAARVAQAEYAQLGQERVDAIVKAIADAAAAQAEQLAALAVEETGFGKVQDKKQKNILASEKLI
EAIKDMKTIGVLNDDKARKVVEIAVPMGVIAGIVPSTNPTSTTIYKSIISLKSGNAIVFTPHPSAKKCIGRTVEIIRRVL
HDCGVSEDLVSVMSVPTIEGSGELMRQADLILATGGPGMVKAAYSSGTPALGVGAGNVPAFIERTADIEDAVAKIFASKT
FDNGTICASEQSIVTEAVIADKVRAALVRNGGYFLEGEKLEKVKRVMERGNGSMNPAIVGRDALHIAKLAGIEVPAGTRL
LISDEPGVGPRYPFSKEKLTALLGFYVVEDWKEACEMCHALLKNGGIGHSLAIHSRNEEVIREFGMKKPVSRMLVNTPST
QGAVGLSTSLFPSFTLGCGAVGGSSTSDNVTPLNLMNVRRIAYDLGTVCCQPAAPAACAQPASSASSSIDVQAITQLIVE
QLKKMA
>Mature_486_residues
MVDKDLLSIQEARALVRAARVAQAEYAQLGQERVDAIVKAIADAAAAQAEQLAALAVEETGFGKVQDKKQKNILASEKLI
EAIKDMKTIGVLNDDKARKVVEIAVPMGVIAGIVPSTNPTSTTIYKSIISLKSGNAIVFTPHPSAKKCIGRTVEIIRRVL
HDCGVSEDLVSVMSVPTIEGSGELMRQADLILATGGPGMVKAAYSSGTPALGVGAGNVPAFIERTADIEDAVAKIFASKT
FDNGTICASEQSIVTEAVIADKVRAALVRNGGYFLEGEKLEKVKRVMERGNGSMNPAIVGRDALHIAKLAGIEVPAGTRL
LISDEPGVGPRYPFSKEKLTALLGFYVVEDWKEACEMCHALLKNGGIGHSLAIHSRNEEVIREFGMKKPVSRMLVNTPST
QGAVGLSTSLFPSFTLGCGAVGGSSTSDNVTPLNLMNVRRIAYDLGTVCCQPAAPAACAQPASSASSSIDVQAITQLIVE
QLKKMA

Specific function: This enzyme has three activities:ADH, ACDH, and PFL- deactivase. In aerobic conditions it acts as a hydrogen peroxide scavenger. The PFL deactivase activity catalyzes the quenching of the pyruvate-formate-lyase catalyst in an iron, NAD, and CoA dependent

COG id: COG1012

COG function: function code C; NAD-dependent aldehyde dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the iron- containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1787493, Length=442, Percent_Identity=47.2850678733032, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1788797, Length=376, Percent_Identity=32.1808510638298, Blast_Score=169, Evalue=5e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR012079 [H]

Pfam domain/function: PF00171 Aldedh; PF00465 Fe-ADH [H]

EC number: =1.1.1.1; =1.2.1.10 [H]

Molecular weight: Translated: 51209; Mature: 51209

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDKDLLSIQEARALVRAARVAQAEYAQLGQERVDAIVKAIADAAAAQAEQLAALAVEET
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GFGKVQDKKQKNILASEKLIEAIKDMKTIGVLNDDKARKVVEIAVPMGVIAGIVPSTNPT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
STTIYKSIISLKSGNAIVFTPHPSAKKCIGRTVEIIRRVLHDCGVSEDLVSVMSVPTIEG
HHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
SGELMRQADLILATGGPGMVKAAYSSGTPALGVGAGNVPAFIERTADIEDAVAKIFASKT
CHHHHHHCCEEEECCCCCCEEEECCCCCCEEECCCCCCCHHHHHHCCHHHHHHHHHHHCC
FDNGTICASEQSIVTEAVIADKVRAALVRNGGYFLEGEKLEKVKRVMERGNGSMNPAIVG
CCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHCCCCCCCEEEC
RDALHIAKLAGIEVPAGTRLLISDEPGVGPRYPFSKEKLTALLGFYVVEDWKEACEMCHA
CHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLKNGGIGHSLAIHSRNEEVIREFGMKKPVSRMLVNTPSTQGAVGLSTSLFPSFTLGCGA
HHHCCCCCCEEEEECCCHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCHHHCCCHHCCCCC
VGGSSTSDNVTPLNLMNVRRIAYDLGTVCCQPAAPAACAQPASSASSSIDVQAITQLIVE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHH
QLKKMA
HHHHCC
>Mature Secondary Structure
MVDKDLLSIQEARALVRAARVAQAEYAQLGQERVDAIVKAIADAAAAQAEQLAALAVEET
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GFGKVQDKKQKNILASEKLIEAIKDMKTIGVLNDDKARKVVEIAVPMGVIAGIVPSTNPT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
STTIYKSIISLKSGNAIVFTPHPSAKKCIGRTVEIIRRVLHDCGVSEDLVSVMSVPTIEG
HHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
SGELMRQADLILATGGPGMVKAAYSSGTPALGVGAGNVPAFIERTADIEDAVAKIFASKT
CHHHHHHCCEEEECCCCCCEEEECCCCCCEEECCCCCCCHHHHHHCCHHHHHHHHHHHCC
FDNGTICASEQSIVTEAVIADKVRAALVRNGGYFLEGEKLEKVKRVMERGNGSMNPAIVG
CCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHCCCCCCCEEEC
RDALHIAKLAGIEVPAGTRLLISDEPGVGPRYPFSKEKLTALLGFYVVEDWKEACEMCHA
CHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLKNGGIGHSLAIHSRNEEVIREFGMKKPVSRMLVNTPSTQGAVGLSTSLFPSFTLGCGA
HHHCCCCCCEEEEECCCHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCHHHCCCHHCCCCC
VGGSSTSDNVTPLNLMNVRRIAYDLGTVCCQPAAPAACAQPASSASSSIDVQAITQLIVE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHH
QLKKMA
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]