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The map label for this gene is appA [H]

Identifier: 78356611

GI number: 78356611

Start: 1590575

End: 1591069

Strand: Direct

Name: appA [H]

Synonym: Dde_1568

Alternate gene names: 78356611

Gene position: 1590575-1591069 (Clockwise)

Preceding gene: 78356610

Following gene: 78356612

Centisome position: 42.64

GC content: 52.93

Gene sequence:

>495_bases
GTGAAGGCGGCTACGATCATCCAGAGCCAGCTTAAGCAGGTTGGCGTGGATGTACGCATCCGCACTGTGGAATGGGCGGC
TTTTATCAAGGAATTTGTGGAAAAAGGGCGTTTTGATGCCATCCTGCTCGGCTGGAATATTCTGGAAGACCCGGATATAT
TCATGGTGTGGCATTCCTCGCAGGCGGTTGCCGGCGGGTTGAACCTGACAAAATACCGTAACGACAGGGTGGATACGCTG
CTTGAGCAGGCTCGCTCAAAGCTTGACCGCGACAGCAGAAAGCCGTTGTATGATGAAGTGCAGCGCATCCTGCACGAAGA
ACAGCCCTATTCCTTTTTGTATGTACCTTATGCTCTGCCCATTGTGCACAGCCGGTTCAAAGGGGTTGAACCCGCGCCCA
AGGGCATAATGCATAATTTCATCCGCTGGTGGGTTCCTGCCGGGCTGCAGCGTTATTCCTCATCCGGACCAGAAGACGGG
ACAAAGAAGAAATAA

Upstream 100 bases:

>100_bases
GCAGGCAGGTTTTGCGGACGCCGACGGGGACGGCATACTGGAGCGCGGGGGCAAGCCTTTTTCATTTACCATACTGACCA
ATCAGGGAAATGATCAGCGC

Downstream 100 bases:

>100_bases
ATGATCCGCATTCAGGACATACTCGATACCATTGCCGAATACAGGCAGGACGCCGACATTGCGCTTATCCAGAAAGCGTA
TGTCTTTTCTGCCGCCGCGC

Product: oligopeptide-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MKAATIIQSQLKQVGVDVRIRTVEWAAFIKEFVEKGRFDAILLGWNILEDPDIFMVWHSSQAVAGGLNLTKYRNDRVDTL
LEQARSKLDRDSRKPLYDEVQRILHEEQPYSFLYVPYALPIVHSRFKGVEPAPKGIMHNFIRWWVPAGLQRYSSSGPEDG
TKKK

Sequences:

>Translated_164_residues
MKAATIIQSQLKQVGVDVRIRTVEWAAFIKEFVEKGRFDAILLGWNILEDPDIFMVWHSSQAVAGGLNLTKYRNDRVDTL
LEQARSKLDRDSRKPLYDEVQRILHEEQPYSFLYVPYALPIVHSRFKGVEPAPKGIMHNFIRWWVPAGLQRYSSSGPEDG
TKKK
>Mature_164_residues
MKAATIIQSQLKQVGVDVRIRTVEWAAFIKEFVEKGRFDAILLGWNILEDPDIFMVWHSSQAVAGGLNLTKYRNDRVDTL
LEQARSKLDRDSRKPLYDEVQRILHEEQPYSFLYVPYALPIVHSRFKGVEPAPKGIMHNFIRWWVPAGLQRYSSSGPEDG
TKKK

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

None

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 18957; Mature: 18957

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAATIIQSQLKQVGVDVRIRTVEWAAFIKEFVEKGRFDAILLGWNILEDPDIFMVWHSS
CCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCEEEECCEECCCCCEEEEEECC
QAVAGGLNLTKYRNDRVDTLLEQARSKLDRDSRKPLYDEVQRILHEEQPYSFLYVPYALP
CHHHCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEHHHHHH
IVHSRFKGVEPAPKGIMHNFIRWWVPAGLQRYSSSGPEDGTKKK
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKAATIIQSQLKQVGVDVRIRTVEWAAFIKEFVEKGRFDAILLGWNILEDPDIFMVWHSS
CCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCEEEECCEECCCCCEEEEEECC
QAVAGGLNLTKYRNDRVDTLLEQARSKLDRDSRKPLYDEVQRILHEEQPYSFLYVPYALP
CHHHCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEHHHHHH
IVHSRFKGVEPAPKGIMHNFIRWWVPAGLQRYSSSGPEDGTKKK
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]