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The map label for this gene is exoA [H]

Identifier: 78355598

GI number: 78355598

Start: 571281

End: 572291

Strand: Direct

Name: exoA [H]

Synonym: Dde_0551

Alternate gene names: 78355598

Gene position: 571281-572291 (Clockwise)

Preceding gene: 78355597

Following gene: 78355601

Centisome position: 15.31

GC content: 55.79

Gene sequence:

>1011_bases
ATGAACAGTCCGGCGGCGGCCCGTACAGTTCTGTTGACGGTCATGGGCAGCTCCTTTTTGCGCCAGCATAGCACAGTCGC
GGCGCTATTGCAGCCGGTATGCTGCAGACACCGGCGGTACGGTTTTATCCTGCCGCGTTCTGTGTTACACACCGTATACG
TGCGCTTTGCAGCGCCATTGCGGCGGGCGTACCTCCTGCGGATATTCACACCGCCCTTTCAGGGCAAAGGCGGATTTGAG
ATAGTGAAGCTTTACTCATGGAACGTGAACGGATTCAGAGCCATCCGGCAAAAACCTGACTGGGACTGGTTTTCCGGCTG
CGATGCTGATGTCATCGGTCTGCAGGAAACCAAAGCCATGCCGGAGCAGATAGACCCTGCGGACCGCAATCCTGCTGGGT
ATCACAGCTACTGGCTGGGAGCGACGGTAAAAAAAGGCTATTCCGGCGTGGCTGCTTTTTCGCGCAGGGAACCGCTTTCG
GTGGCGTATGATCTGCCCGTGGAGCAGTATCAGGGCGAGGGGCGCGTCATCATGCTGGAATACGATGCCTTTTACTACTT
TAACATCTATTTTCCCAACGGACAGAGCGGCGACGAGCGGCTGCAGTACAAACTGGGCTTTTATGACGCTTTTCTTGAGC
ATGCGCAGACCCTGCGCCGGAAAAAGCCCATCGTGGTATGCGGCGACTTTAATACGGCGCATCGCCCTGTCGACCTTGCC
CGTCCCAGAGAAAACGAGGGCACGTCAGGGTTTCTGCCCGTGGAGCGGGCATGGATGGACAAGTTTATAGACCACGGCTA
CGTGGATACCTTCAGACTGGTGCATCCCGACGCGCAAGCCATGTATTCATGGTGGTCATACCGGTTTAAAGCGCGCGACA
GAAATGTGGGCTGGCGCATCGACTATTTTTTTGTTTCCGATGAATTACGCCATGCCGTAAAGGATGCCACCATCGAAATG
GAGGTTTTCGGTTCGGACCATTGTCCCGTGGGGCTTGAACTGGCACTGTGA

Upstream 100 bases:

>100_bases
CCAGTCGGTAAATGCCGACTGCAGCAGATTTATACCCACAAAAGCAGTGAAACCGAGCCAGTAGGGCGAGTGCAGCCATG
CCAGCAGCAGACTGAGCAGA

Downstream 100 bases:

>100_bases
CGCGGCCATGCATACAGCACCGCGCGGCAGACAGGCCGCAGGCGCGGGGCATGCCGTATGCGGCCGCAGCACGCGGCACG
GCGCAGCCGTACAGCCGGCT

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MNSPAAARTVLLTVMGSSFLRQHSTVAALLQPVCCRHRRYGFILPRSVLHTVYVRFAAPLRRAYLLRIFTPPFQGKGGFE
IVKLYSWNVNGFRAIRQKPDWDWFSGCDADVIGLQETKAMPEQIDPADRNPAGYHSYWLGATVKKGYSGVAAFSRREPLS
VAYDLPVEQYQGEGRVIMLEYDAFYYFNIYFPNGQSGDERLQYKLGFYDAFLEHAQTLRRKKPIVVCGDFNTAHRPVDLA
RPRENEGTSGFLPVERAWMDKFIDHGYVDTFRLVHPDAQAMYSWWSYRFKARDRNVGWRIDYFFVSDELRHAVKDATIEM
EVFGSDHCPVGLELAL

Sequences:

>Translated_336_residues
MNSPAAARTVLLTVMGSSFLRQHSTVAALLQPVCCRHRRYGFILPRSVLHTVYVRFAAPLRRAYLLRIFTPPFQGKGGFE
IVKLYSWNVNGFRAIRQKPDWDWFSGCDADVIGLQETKAMPEQIDPADRNPAGYHSYWLGATVKKGYSGVAAFSRREPLS
VAYDLPVEQYQGEGRVIMLEYDAFYYFNIYFPNGQSGDERLQYKLGFYDAFLEHAQTLRRKKPIVVCGDFNTAHRPVDLA
RPRENEGTSGFLPVERAWMDKFIDHGYVDTFRLVHPDAQAMYSWWSYRFKARDRNVGWRIDYFFVSDELRHAVKDATIEM
EVFGSDHCPVGLELAL
>Mature_336_residues
MNSPAAARTVLLTVMGSSFLRQHSTVAALLQPVCCRHRRYGFILPRSVLHTVYVRFAAPLRRAYLLRIFTPPFQGKGGFE
IVKLYSWNVNGFRAIRQKPDWDWFSGCDADVIGLQETKAMPEQIDPADRNPAGYHSYWLGATVKKGYSGVAAFSRREPLS
VAYDLPVEQYQGEGRVIMLEYDAFYYFNIYFPNGQSGDERLQYKLGFYDAFLEHAQTLRRKKPIVVCGDFNTAHRPVDLA
RPRENEGTSGFLPVERAWMDKFIDHGYVDTFRLVHPDAQAMYSWWSYRFKARDRNVGWRIDYFFVSDELRHAVKDATIEM
EVFGSDHCPVGLELAL

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=272, Percent_Identity=39.7058823529412, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI18375503, Length=272, Percent_Identity=39.7058823529412, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI18375501, Length=272, Percent_Identity=39.7058823529412, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI18375507, Length=321, Percent_Identity=26.791277258567, Blast_Score=105, Evalue=7e-23,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=33.0827067669173, Blast_Score=134, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI71989536, Length=277, Percent_Identity=33.2129963898917, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI221330655, Length=251, Percent_Identity=37.8486055776892, Blast_Score=187, Evalue=9e-48,
Organism=Drosophila melanogaster, GI17136678, Length=251, Percent_Identity=37.8486055776892, Blast_Score=186, Evalue=2e-47,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 38726; Mature: 38726

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSPAAARTVLLTVMGSSFLRQHSTVAALLQPVCCRHRRYGFILPRSVLHTVYVRFAAPL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHH
RRAYLLRIFTPPFQGKGGFEIVKLYSWNVNGFRAIRQKPDWDWFSGCDADVIGLQETKAM
HHEEEEEEECCCCCCCCCEEEEEEEEECCCHHHHHHCCCCCCHHCCCCCCEECCHHHHCC
PEQIDPADRNPAGYHSYWLGATVKKGYSGVAAFSRREPLSVAYDLPVEQYQGEGRVIMLE
CCCCCCCCCCCCCCCEEECCCHHHCCCCCHHHHCCCCCCEEEEECCHHHHCCCCCEEEEE
YDAFYYFNIYFPNGQSGDERLQYKLGFYDAFLEHAQTLRRKKPIVVCGDFNTAHRPVDLA
ECEEEEEEEEECCCCCCCHHEEEEHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCC
RPRENEGTSGFLPVERAWMDKFIDHGYVDTFRLVHPDAQAMYSWWSYRFKARDRNVGWRI
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHEEEEECCCCCCEEE
DYFFVSDELRHAVKDATIEMEVFGSDHCPVGLELAL
EEEEECHHHHHHHHCCEEEEEEECCCCCCCCEEEEC
>Mature Secondary Structure
MNSPAAARTVLLTVMGSSFLRQHSTVAALLQPVCCRHRRYGFILPRSVLHTVYVRFAAPL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHH
RRAYLLRIFTPPFQGKGGFEIVKLYSWNVNGFRAIRQKPDWDWFSGCDADVIGLQETKAM
HHEEEEEEECCCCCCCCCEEEEEEEEECCCHHHHHHCCCCCCHHCCCCCCEECCHHHHCC
PEQIDPADRNPAGYHSYWLGATVKKGYSGVAAFSRREPLSVAYDLPVEQYQGEGRVIMLE
CCCCCCCCCCCCCCCEEECCCHHHCCCCCHHHHCCCCCCEEEEECCHHHHCCCCCEEEEE
YDAFYYFNIYFPNGQSGDERLQYKLGFYDAFLEHAQTLRRKKPIVVCGDFNTAHRPVDLA
ECEEEEEEEEECCCCCCCHHEEEEHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCC
RPRENEGTSGFLPVERAWMDKFIDHGYVDTFRLVHPDAQAMYSWWSYRFKARDRNVGWRI
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHEEEEECCCCCCEEE
DYFFVSDELRHAVKDATIEMEVFGSDHCPVGLELAL
EEEEECHHHHHHHHCCEEEEEEECCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]