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The map label for this gene is glpF [H]

Identifier: 78355456

GI number: 78355456

Start: 404211

End: 404966

Strand: Direct

Name: glpF [H]

Synonym: Dde_0409

Alternate gene names: 78355456

Gene position: 404211-404966 (Clockwise)

Preceding gene: 78355455

Following gene: 78355457

Centisome position: 10.84

GC content: 58.86

Gene sequence:

>756_bases
ATGAAGGAACTGTCTCTGGGTAAGGAACTCGTTTCCGAGTTCATGGGCACACTGGTGCTCATTCTTTTCGGTACCGGTAC
GGTAGCCATGACCGTGCTGTTCGGCGAAGCCCTTAACATCACCTGGGCAAACATCACCCTCGGGTGGGGTCTTGCGGTGC
TTCTGGGCGTCATGGCCGGTCTGCCTTCCGGTGCACACATCAACCCTGCGGTCACTCTGGGGCTGGCTGTGACGGGCCGC
TTCCCCTGGCGCAAAGTGGTGCCCTTCACCCTTGCCCAGACCGCAGGCGCATTTGTGGGTGCGGCTCTGGTTTTTATCGA
CTTCAAGGCCAAGTGGCTGCTCGTGGACCCCAATCTGGCCAGTACTGCCGGTATTTTCTGCACCTTCCCCGCCATTCCCG
ATTCGTTCATGCCCGGCTTCATCGACCAGATAATCGGCACCGCCATTCTGCTTTTCGGCATTCTGGCTGCCGGTGACTTT
GCCGCAAAAAATAATGCCGGCTGGTCCGTGCCTTTCATCGTTGCTCTGCTGGTTACCGTCATCGGTATGGCTCTGGGCGC
CATGAACGGGTACGCCATCAACCCCGCCCGTGATTTCGGTCCGCGTCTTTTCGCGCTGGTTGCCGGATTCACGCAGGCAA
ACCTGATGGATATTTCCATCGTGCTCACGCCCATCATCGGTCCTTTCATCGGCGGTGCCGTGGGTGCCGTGGTGTACGAC
TGGACCACCGGCGCCGTGCATAAGGCTCAGAACTAA

Upstream 100 bases:

>100_bases
GCAGGGCAGCGAAGGGTAGCAACGGGGATTGTATCTGTGACTCGGAAAATGCGGCGCAATGGGCGGCGCACCAACATGAC
TCAAGGATTGCGGAGGATGT

Downstream 100 bases:

>100_bases
CAACGTGTAATTTCCCGCAAGGAGGACGAAATGAGCAAATACATTCTGGCACTCGATCAGGGCACAACCAGCTCCCGCGC
CATTCTGTTCACCCGCGAGG

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MKELSLGKELVSEFMGTLVLILFGTGTVAMTVLFGEALNITWANITLGWGLAVLLGVMAGLPSGAHINPAVTLGLAVTGR
FPWRKVVPFTLAQTAGAFVGAALVFIDFKAKWLLVDPNLASTAGIFCTFPAIPDSFMPGFIDQIIGTAILLFGILAAGDF
AAKNNAGWSVPFIVALLVTVIGMALGAMNGYAINPARDFGPRLFALVAGFTQANLMDISIVLTPIIGPFIGGAVGAVVYD
WTTGAVHKAQN

Sequences:

>Translated_251_residues
MKELSLGKELVSEFMGTLVLILFGTGTVAMTVLFGEALNITWANITLGWGLAVLLGVMAGLPSGAHINPAVTLGLAVTGR
FPWRKVVPFTLAQTAGAFVGAALVFIDFKAKWLLVDPNLASTAGIFCTFPAIPDSFMPGFIDQIIGTAILLFGILAAGDF
AAKNNAGWSVPFIVALLVTVIGMALGAMNGYAINPARDFGPRLFALVAGFTQANLMDISIVLTPIIGPFIGGAVGAVVYD
WTTGAVHKAQN
>Mature_251_residues
MKELSLGKELVSEFMGTLVLILFGTGTVAMTVLFGEALNITWANITLGWGLAVLLGVMAGLPSGAHINPAVTLGLAVTGR
FPWRKVVPFTLAQTAGAFVGAALVFIDFKAKWLLVDPNLASTAGIFCTFPAIPDSFMPGFIDQIIGTAILLFGILAAGDF
AAKNNAGWSVPFIVALLVTVIGMALGAMNGYAINPARDFGPRLFALVAGFTQANLMDISIVLTPIIGPFIGGAVGAVVYD
WTTGAVHKAQN

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=261, Percent_Identity=41.7624521072797, Blast_Score=154, Evalue=9e-38,
Organism=Homo sapiens, GI157266307, Length=253, Percent_Identity=38.7351778656126, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI22538420, Length=254, Percent_Identity=34.251968503937, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI4502187, Length=253, Percent_Identity=34.3873517786561, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI310133356, Length=218, Percent_Identity=33.9449541284404, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI310114181, Length=218, Percent_Identity=33.9449541284404, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1790362, Length=254, Percent_Identity=39.3700787401575, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1787101, Length=105, Percent_Identity=40, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71994009, Length=249, Percent_Identity=36.144578313253, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71992966, Length=248, Percent_Identity=32.258064516129, Blast_Score=120, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17544068, Length=256, Percent_Identity=33.984375, Blast_Score=116, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17531429, Length=266, Percent_Identity=34.2105263157895, Blast_Score=114, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17531431, Length=266, Percent_Identity=34.2105263157895, Blast_Score=114, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17533613, Length=247, Percent_Identity=32.3886639676113, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32564052, Length=247, Percent_Identity=32.3886639676113, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71992961, Length=248, Percent_Identity=28.2258064516129, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17558372, Length=243, Percent_Identity=28.8065843621399, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321054, Length=210, Percent_Identity=37.6190476190476, Blast_Score=122, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6322985, Length=280, Percent_Identity=30.3571428571429, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 26127; Mature: 26127

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELSLGKELVSEFMGTLVLILFGTGTVAMTVLFGEALNITWANITLGWGLAVLLGVMAG
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHEEEEEEEEHHHHHHHHHHHHHC
LPSGAHINPAVTLGLAVTGRFPWRKVVPFTLAQTAGAFVGAALVFIDFKAKWLLVDPNLA
CCCCCCCCHHEEEEEEEECCCCCHHHCCHHHHHHHHHHHHHHHHEEEECCEEEEECCCCC
STAGIFCTFPAIPDSFMPGFIDQIIGTAILLFGILAAGDFAAKNNAGWSVPFIVALLVTV
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
IGMALGAMNGYAINPARDFGPRLFALVAGFTQANLMDISIVLTPIIGPFIGGAVGAVVYD
HHHHHHCCCCEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEE
WTTGAVHKAQN
CCCCCCCCCCC
>Mature Secondary Structure
MKELSLGKELVSEFMGTLVLILFGTGTVAMTVLFGEALNITWANITLGWGLAVLLGVMAG
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHEEEEEEEEHHHHHHHHHHHHHC
LPSGAHINPAVTLGLAVTGRFPWRKVVPFTLAQTAGAFVGAALVFIDFKAKWLLVDPNLA
CCCCCCCCHHEEEEEEEECCCCCHHHCCHHHHHHHHHHHHHHHHEEEECCEEEEECCCCC
STAGIFCTFPAIPDSFMPGFIDQIIGTAILLFGILAAGDFAAKNNAGWSVPFIVALLVTV
CCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
IGMALGAMNGYAINPARDFGPRLFALVAGFTQANLMDISIVLTPIIGPFIGGAVGAVVYD
HHHHHHCCCCEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEE
WTTGAVHKAQN
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]