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The map label for this gene is glpK

Identifier: 78355457

GI number: 78355457

Start: 404997

End: 406490

Strand: Direct

Name: glpK

Synonym: Dde_0410

Alternate gene names: 78355457

Gene position: 404997-406490 (Clockwise)

Preceding gene: 78355456

Following gene: 78355460

Centisome position: 10.86

GC content: 59.91

Gene sequence:

>1494_bases
ATGAGCAAATACATTCTGGCACTCGATCAGGGCACAACCAGCTCCCGCGCCATTCTGTTCACCCGCGAGGGCGACATCAA
GCAGATCGCCCAGAAAGAGTTCACGCAGATATACCCCCAGCCCGGCTGGGTTGAGCATAATGCCAACGAAATATTCGACA
CCCAGTCGTGGGTGATGACCGAATGCCTGCAGCGTGCCGGCGTCAACGCCAGCGAAGTGGCCGCCATAGGCATCACCAAC
CAGCGTGAAACCACCGTGGTGTGGGACAGAAAAAGCGGCGCTCCCGTGCACAACGCCATTGTGTGGCAGGACCGCCGTAC
CGCCTCCATCTGCGATGAAATGAAAAAACGCGGACTGGCGGAAACCATCCGCGAAAAAACCGGCCTTGTCATCGACGCCT
ATTTTTCGGGCACCAAGGTCAAATGGATTCTGGATAACGTGCCCGGCGCCCGTGCAAAGGCGGAACGCGGCGAACTGTGC
TTCGGCACCATCGACACGTGGGTTATCTGGAACCTGACCAAAGGCAAGGAACACGTCACCGACGAATCCAACGCCAGCCG
TACCATGCTGTTCAACATCCACACCGGTGACTGGGATGAAGAACTGCTGAAGATTCTGGATGTTCCGCGTTCCATGCTGC
CCCGCGTGGCCGGTTCTTCCGAAGTGGTGGCCGAAACGCATCCCGAGTTCCTCGGCAAGGCTGTGCCGGTTTCCGGCATC
GCCGGCGACCAGCAGGCTGCCACCTTCGGCAACGCCTGCCTTACCGAAGGGATGGCCAAAAACACCTACGGTACAGGCTG
CTTCATGCTGCTGAACACCGGCAAGCAGGCTCACATCAGCAAAAACAACCTGCTGACCACCACCGGTTGGAACACGCCTT
CCGGCCGTTACTACTGCCTTGAAGGCAGCGTGTTCATCGCCGGTGCCGTGGTGCAGTGGCTGCGTGACGGTCTGGGGATT
ATCCAGAGCGCTCCCGAAGTGGAACAGCTGGCACTGAGTGTGCCGGACAACGGCGGTGTGGTGCTTGTGCCTGCATTTGC
CGGTCTGGGTGCACCGCACTGGGACCAGTACGCACGCGGCACCATGGTGGGCGTCACCCGCGGTACCACCAAGGCGCATA
TAGCCCGTGCCGCGCTGGAATCCATAGCACTGCAGACGCTGGATATCATGGACTGCATGCAGAAGGACGCCGGAATCAAT
CTGGCTGCCCTGCGTGCGGACGGCGGTGCCACCCGCAACAACCTGCTCATGCAGTTCCAGGCAGATGTGCTCGGTGTGCC
TGTCGAGCGTCCCAAAGTGACGGAAACCACCGCGCTGGGCGCTGCGTATCTGGCAGGTCTTGCCGTGGGCTTCTGGAAGA
GCGAAGACGAAATCACCGCCATGTGGCAGCTTGACCGTCGTTTTGAGCCGAACATGAGCGCCGAAGTGCGTGAAAAGCTG
GTCTACAACTGGAAGCGCGCCGTGGAACGTTCCAAGGAGTGGGCGGAAGACTAA

Upstream 100 bases:

>100_bases
CATCGGCGGTGCCGTGGGTGCCGTGGTGTACGACTGGACCACCGGCGCCGTGCATAAGGCTCAGAACTAACAACGTGTAA
TTTCCCGCAAGGAGGACGAA

Downstream 100 bases:

>100_bases
CCGCCGCGTACTGATATGACCATACGGTGCGTCCGCAGCGGCGTGCCATGAAAGTGCAGGCACCCCCGTCCGCAACAGGG
CGGGGGTGCCGTATTATTGG

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MSKYILALDQGTTSSRAILFTREGDIKQIAQKEFTQIYPQPGWVEHNANEIFDTQSWVMTECLQRAGVNASEVAAIGITN
QRETTVVWDRKSGAPVHNAIVWQDRRTASICDEMKKRGLAETIREKTGLVIDAYFSGTKVKWILDNVPGARAKAERGELC
FGTIDTWVIWNLTKGKEHVTDESNASRTMLFNIHTGDWDEELLKILDVPRSMLPRVAGSSEVVAETHPEFLGKAVPVSGI
AGDQQAATFGNACLTEGMAKNTYGTGCFMLLNTGKQAHISKNNLLTTTGWNTPSGRYYCLEGSVFIAGAVVQWLRDGLGI
IQSAPEVEQLALSVPDNGGVVLVPAFAGLGAPHWDQYARGTMVGVTRGTTKAHIARAALESIALQTLDIMDCMQKDAGIN
LAALRADGGATRNNLLMQFQADVLGVPVERPKVTETTALGAAYLAGLAVGFWKSEDEITAMWQLDRRFEPNMSAEVREKL
VYNWKRAVERSKEWAED

Sequences:

>Translated_497_residues
MSKYILALDQGTTSSRAILFTREGDIKQIAQKEFTQIYPQPGWVEHNANEIFDTQSWVMTECLQRAGVNASEVAAIGITN
QRETTVVWDRKSGAPVHNAIVWQDRRTASICDEMKKRGLAETIREKTGLVIDAYFSGTKVKWILDNVPGARAKAERGELC
FGTIDTWVIWNLTKGKEHVTDESNASRTMLFNIHTGDWDEELLKILDVPRSMLPRVAGSSEVVAETHPEFLGKAVPVSGI
AGDQQAATFGNACLTEGMAKNTYGTGCFMLLNTGKQAHISKNNLLTTTGWNTPSGRYYCLEGSVFIAGAVVQWLRDGLGI
IQSAPEVEQLALSVPDNGGVVLVPAFAGLGAPHWDQYARGTMVGVTRGTTKAHIARAALESIALQTLDIMDCMQKDAGIN
LAALRADGGATRNNLLMQFQADVLGVPVERPKVTETTALGAAYLAGLAVGFWKSEDEITAMWQLDRRFEPNMSAEVREKL
VYNWKRAVERSKEWAED
>Mature_496_residues
SKYILALDQGTTSSRAILFTREGDIKQIAQKEFTQIYPQPGWVEHNANEIFDTQSWVMTECLQRAGVNASEVAAIGITNQ
RETTVVWDRKSGAPVHNAIVWQDRRTASICDEMKKRGLAETIREKTGLVIDAYFSGTKVKWILDNVPGARAKAERGELCF
GTIDTWVIWNLTKGKEHVTDESNASRTMLFNIHTGDWDEELLKILDVPRSMLPRVAGSSEVVAETHPEFLGKAVPVSGIA
GDQQAATFGNACLTEGMAKNTYGTGCFMLLNTGKQAHISKNNLLTTTGWNTPSGRYYCLEGSVFIAGAVVQWLRDGLGII
QSAPEVEQLALSVPDNGGVVLVPAFAGLGAPHWDQYARGTMVGVTRGTTKAHIARAALESIALQTLDIMDCMQKDAGINL
AALRADGGATRNNLLMQFQADVLGVPVERPKVTETTALGAAYLAGLAVGFWKSEDEITAMWQLDRRFEPNMSAEVREKLV
YNWKRAVERSKEWAED

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI189409120, Length=505, Percent_Identity=44.5544554455446, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI4504007, Length=505, Percent_Identity=44.5544554455446, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI41393575, Length=507, Percent_Identity=43.5897435897436, Blast_Score=418, Evalue=1e-117,
Organism=Homo sapiens, GI42794763, Length=510, Percent_Identity=44.3137254901961, Blast_Score=417, Evalue=1e-116,
Organism=Homo sapiens, GI88196792, Length=507, Percent_Identity=31.9526627218935, Blast_Score=300, Evalue=2e-81,
Organism=Escherichia coli, GI1790361, Length=495, Percent_Identity=58.1818181818182, Blast_Score=605, Evalue=1e-174,
Organism=Escherichia coli, GI1790005, Length=489, Percent_Identity=25.9713701431493, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789168, Length=499, Percent_Identity=25.250501002004, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1787789, Length=477, Percent_Identity=25.3668763102725, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1789987, Length=478, Percent_Identity=24.2677824267782, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17535599, Length=503, Percent_Identity=41.948310139165, Blast_Score=378, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71986140, Length=519, Percent_Identity=26.7822736030828, Blast_Score=195, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI71986147, Length=300, Percent_Identity=32.6666666666667, Blast_Score=144, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6321755, Length=559, Percent_Identity=36.3148479427549, Blast_Score=314, Evalue=2e-86,
Organism=Drosophila melanogaster, GI17864214, Length=509, Percent_Identity=43.4184675834971, Blast_Score=394, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24655555, Length=499, Percent_Identity=40.0801603206413, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655560, Length=499, Percent_Identity=40.0801603206413, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655570, Length=499, Percent_Identity=40.0801603206413, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655574, Length=499, Percent_Identity=40.0801603206413, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24655565, Length=499, Percent_Identity=40.0801603206413, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24654573, Length=445, Percent_Identity=45.1685393258427, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI19922058, Length=530, Percent_Identity=31.3207547169811, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24652871, Length=497, Percent_Identity=31.7907444668008, Blast_Score=249, Evalue=3e-66,
Organism=Drosophila melanogaster, GI24656367, Length=514, Percent_Identity=28.988326848249, Blast_Score=231, Evalue=9e-61,
Organism=Drosophila melanogaster, GI21357867, Length=533, Percent_Identity=28.5178236397749, Blast_Score=227, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24656372, Length=360, Percent_Identity=31.3888888888889, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI45552909, Length=301, Percent_Identity=29.9003322259136, Blast_Score=132, Evalue=7e-31,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_DESDG (Q316D5)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_386906.1
- ProteinModelPortal:   Q316D5
- SMR:   Q316D5
- STRING:   Q316D5
- GeneID:   3756303
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_0410
- NMPDR:   fig|207559.3.peg.1950
- eggNOG:   COG0554
- HOGENOM:   HBG511469
- OMA:   KPSSEVY
- ProtClustDB:   CLSK706192
- BioCyc:   DDES207559:DDE_0410-MONOMER
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 54554; Mature: 54422

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 12-12 BINDING 16-16 BINDING 82-82 BINDING 134-134 BINDING 243-243 BINDING 265-265 BINDING 308-308

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKYILALDQGTTSSRAILFTREGDIKQIAQKEFTQIYPQPGWVEHNANEIFDTQSWVMT
CCCEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHH
ECLQRAGVNASEVAAIGITNQRETTVVWDRKSGAPVHNAIVWQDRRTASICDEMKKRGLA
HHHHHCCCCHHHEEEEECCCCCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHCCHH
ETIREKTGLVIDAYFSGTKVKWILDNVPGARAKAERGELCFGTIDTWVIWNLTKGKEHVT
HHHHHHCCEEEEEEECCCEEEEEEECCCCCCCCCCCCCEEEEEECEEEEEEECCCCHHCC
DESNASRTMLFNIHTGDWDEELLKILDVPRSMLPRVAGSSEVVAETHPEFLGKAVPVSGI
CCCCCCEEEEEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCHHHHCCHHHHCCCCCCCCC
AGDQQAATFGNACLTEGMAKNTYGTGCFMLLNTGKQAHISKNNLLTTTGWNTPSGRYYCL
CCCCHHHHHCCHHHHCCCCCCCCCCEEEEEEECCCEEEECCCCEEEECCCCCCCCCEEEE
EGSVFIAGAVVQWLRDGLGIIQSAPEVEQLALSVPDNGGVVLVPAFAGLGAPHWDQYARG
ECCEEHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCCCEEEEECCCCCCCCCHHHHHCC
TMVGVTRGTTKAHIARAALESIALQTLDIMDCMQKDAGINLAALRADGGATRNNLLMQFQ
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEE
ADVLGVPVERPKVTETTALGAAYLAGLAVGFWKSEDEITAMWQLDRRFEPNMSAEVREKL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHH
VYNWKRAVERSKEWAED
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKYILALDQGTTSSRAILFTREGDIKQIAQKEFTQIYPQPGWVEHNANEIFDTQSWVMT
CCEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHH
ECLQRAGVNASEVAAIGITNQRETTVVWDRKSGAPVHNAIVWQDRRTASICDEMKKRGLA
HHHHHCCCCHHHEEEEECCCCCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHCCHH
ETIREKTGLVIDAYFSGTKVKWILDNVPGARAKAERGELCFGTIDTWVIWNLTKGKEHVT
HHHHHHCCEEEEEEECCCEEEEEEECCCCCCCCCCCCCEEEEEECEEEEEEECCCCHHCC
DESNASRTMLFNIHTGDWDEELLKILDVPRSMLPRVAGSSEVVAETHPEFLGKAVPVSGI
CCCCCCEEEEEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCHHHHCCHHHHCCCCCCCCC
AGDQQAATFGNACLTEGMAKNTYGTGCFMLLNTGKQAHISKNNLLTTTGWNTPSGRYYCL
CCCCHHHHHCCHHHHCCCCCCCCCCEEEEEEECCCEEEECCCCEEEECCCCCCCCCEEEE
EGSVFIAGAVVQWLRDGLGIIQSAPEVEQLALSVPDNGGVVLVPAFAGLGAPHWDQYARG
ECCEEHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCCCEEEEECCCCCCCCCHHHHHCC
TMVGVTRGTTKAHIARAALESIALQTLDIMDCMQKDAGINLAALRADGGATRNNLLMQFQ
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEE
ADVLGVPVERPKVTETTALGAAYLAGLAVGFWKSEDEITAMWQLDRRFEPNMSAEVREKL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHH
VYNWKRAVERSKEWAED
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA