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The map label for this gene is dus1 [H]

Identifier: 78355436

GI number: 78355436

Start: 382941

End: 384077

Strand: Direct

Name: dus1 [H]

Synonym: Dde_0389

Alternate gene names: 78355436

Gene position: 382941-384077 (Clockwise)

Preceding gene: 78355435

Following gene: 78355437

Centisome position: 10.27

GC content: 63.94

Gene sequence:

>1137_bases
ATGAACATGATGAAGGTTTCCCGCGAGTCTTGTTTTGATGCGGCATCAGTGGTGCCCGGCGTGCCATCCGGTTTTGCACA
GCCCGTGGTTTCTGACGGGCTGACGGTGCTGCCCGCCGGCGGTGATGCCCCGTGGCTTGCCCCGCTTGCCGGGTTCACCG
ATCTGGCGTTTCGCCTGCTGTGCCGTGAATTCGGCGCGCGTGTTGTCTGTTCCGAGATGGTCAGCGCCAAGGGGCTGGTA
TACCGGAGTCCGGGTACGCATGCTCTGCTGGAAACCTGTCCGCAGGACGCGCCCATGGTGCTGCAGCTGTTCGGCAACGA
GGTGCCCATCATGGATGCGGCCATGCGCGAACTGGTGGACAAGGGATACCGCTGGTTTGACCTCAACATGGGCTGCTCTG
TGCGCAAGGTGGTCAAAACCGGCTGCGGCGCGGGTATGCTGCGCGATGTGCCCAATGCTGTCCGTGTGGCCCGCGCCATG
ACGGCCGTGGCGGGGGCGGGCCGTGTGGGGTTCAAGTTCCGGCTGGGCTGGGAACAGGGGGCACAGGTCTGCATGGACCT
TGCCCGCAGGCTGGAGGACGCCGGAGCTGGCTGGCTTACCCTGCATCCGCGGTTTGCCCGGCAGGGATTCAGCGGGACGG
CGGACTGGGATGCGCTGGCGCGTCTCAAGGAGCATGTGACTATTCCCGTCATTGCCAGCGGCGACCTGTTTACCGCCGGA
GATGCTGTGCGCTGCATGCGTGCCAGCGGGGTGGACGGAGTCATGTTTGCCCGCGGGGCACTGGCCAATCCTGCCGTGTT
CGAAGATTTTTCCGCCATGATGTCCGGCGGTGAGCAGCCGCAGCCCGACCCTGAAAGGACTGTGCGCATCATACGTCGGC
ATGTGGCACTGGCAAGGGCGCTGTGCGCCGAAAAAACAGCGCTGTTCAAAATGCGGACTTTTGTGCCCCGCTATGTACGG
CAGTTTCCCGGAGCGCGTGCGCTGCGCAACCATCTGGCCTCGTGCCTGACATGGGAAGGGCTGGACCATCTGCTGGATAC
TTTTTTTGAGCGGTGGCGGGCGCAGGGCGCGCCCATGCTGGATGACGGTGCATGCCGTGTTCAGACCGCCCCCGCGGGAG
ACGATCAATGCATGTAA

Upstream 100 bases:

>100_bases
AACGCCTGACATTTTTTCCGGGGGACGTCCCCGCTTTTCTGATGCAGGGCGGCAATGCGGGCTATACTGCCCCGCGGGGC
CGCATCTGATATTTTGTGGA

Downstream 100 bases:

>100_bases
AACGTGCGGCCACTGCCGGTTTTTGCATGGGCGTGGGACTTGCCCTGAAAAAACTGGACAGGGAACTTGATAATAACGCG
AAAGATATAGCCACGCTCGG

Product: dihydrouridine synthase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MNMMKVSRESCFDAASVVPGVPSGFAQPVVSDGLTVLPAGGDAPWLAPLAGFTDLAFRLLCREFGARVVCSEMVSAKGLV
YRSPGTHALLETCPQDAPMVLQLFGNEVPIMDAAMRELVDKGYRWFDLNMGCSVRKVVKTGCGAGMLRDVPNAVRVARAM
TAVAGAGRVGFKFRLGWEQGAQVCMDLARRLEDAGAGWLTLHPRFARQGFSGTADWDALARLKEHVTIPVIASGDLFTAG
DAVRCMRASGVDGVMFARGALANPAVFEDFSAMMSGGEQPQPDPERTVRIIRRHVALARALCAEKTALFKMRTFVPRYVR
QFPGARALRNHLASCLTWEGLDHLLDTFFERWRAQGAPMLDDGACRVQTAPAGDDQCM

Sequences:

>Translated_378_residues
MNMMKVSRESCFDAASVVPGVPSGFAQPVVSDGLTVLPAGGDAPWLAPLAGFTDLAFRLLCREFGARVVCSEMVSAKGLV
YRSPGTHALLETCPQDAPMVLQLFGNEVPIMDAAMRELVDKGYRWFDLNMGCSVRKVVKTGCGAGMLRDVPNAVRVARAM
TAVAGAGRVGFKFRLGWEQGAQVCMDLARRLEDAGAGWLTLHPRFARQGFSGTADWDALARLKEHVTIPVIASGDLFTAG
DAVRCMRASGVDGVMFARGALANPAVFEDFSAMMSGGEQPQPDPERTVRIIRRHVALARALCAEKTALFKMRTFVPRYVR
QFPGARALRNHLASCLTWEGLDHLLDTFFERWRAQGAPMLDDGACRVQTAPAGDDQCM
>Mature_378_residues
MNMMKVSRESCFDAASVVPGVPSGFAQPVVSDGLTVLPAGGDAPWLAPLAGFTDLAFRLLCREFGARVVCSEMVSAKGLV
YRSPGTHALLETCPQDAPMVLQLFGNEVPIMDAAMRELVDKGYRWFDLNMGCSVRKVVKTGCGAGMLRDVPNAVRVARAM
TAVAGAGRVGFKFRLGWEQGAQVCMDLARRLEDAGAGWLTLHPRFARQGFSGTADWDALARLKEHVTIPVIASGDLFTAG
DAVRCMRASGVDGVMFARGALANPAVFEDFSAMMSGGEQPQPDPERTVRIIRRHVALARALCAEKTALFKMRTFVPRYVR
QFPGARALRNHLASCLTWEGLDHLLDTFFERWRAQGAPMLDDGACRVQTAPAGDDQCM

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=252, Percent_Identity=28.5714285714286, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI40807366, Length=303, Percent_Identity=30.3630363036304, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI239788462, Length=230, Percent_Identity=30, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI239788483, Length=230, Percent_Identity=30, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI8923374, Length=240, Percent_Identity=23.3333333333333, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1789660, Length=305, Percent_Identity=29.8360655737705, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1788462, Length=299, Percent_Identity=28.4280936454849, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17507177, Length=231, Percent_Identity=32.9004329004329, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI25144369, Length=219, Percent_Identity=31.9634703196347, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17543114, Length=232, Percent_Identity=26.2931034482759, Blast_Score=91, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6323437, Length=232, Percent_Identity=29.7413793103448, Blast_Score=112, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6323560, Length=309, Percent_Identity=27.1844660194175, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323433, Length=264, Percent_Identity=24.2424242424242, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24580595, Length=266, Percent_Identity=31.5789473684211, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI19920448, Length=266, Percent_Identity=31.5789473684211, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI19921524, Length=226, Percent_Identity=33.1858407079646, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24585320, Length=229, Percent_Identity=28.3842794759825, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 40988; Mature: 40988

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
7.9 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
7.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMMKVSRESCFDAASVVPGVPSGFAQPVVSDGLTVLPAGGDAPWLAPLAGFTDLAFRLL
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHH
CREFGARVVCSEMVSAKGLVYRSPGTHALLETCPQDAPMVLQLFGNEVPIMDAAMRELVD
HHHHCCHHHHHHHHHHCCEEEECCCCHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHH
KGYRWFDLNMGCSVRKVVKTGCGAGMLRDVPNAVRVARAMTAVAGAGRVGFKFRLGWEQG
CCCEEEECCCCCHHHHHHHHCCCCCHHHHCHHHHHHHHHHHHHHCCCCCCEEEEECHHHH
AQVCMDLARRLEDAGAGWLTLHPRFARQGFSGTADWDALARLKEHVTIPVIASGDLFTAG
HHHHHHHHHHHHHCCCCEEEECHHHHHCCCCCCCCHHHHHHHHHCCEEEEEECCCEEECC
DAVRCMRASGVDGVMFARGALANPAVFEDFSAMMSGGEQPQPDPERTVRIIRRHVALARA
HHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
LCAEKTALFKMRTFVPRYVRQFPGARALRNHLASCLTWEGLDHLLDTFFERWRAQGAPML
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DDGACRVQTAPAGDDQCM
CCCCCEEEECCCCCCCCC
>Mature Secondary Structure
MNMMKVSRESCFDAASVVPGVPSGFAQPVVSDGLTVLPAGGDAPWLAPLAGFTDLAFRLL
CCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHH
CREFGARVVCSEMVSAKGLVYRSPGTHALLETCPQDAPMVLQLFGNEVPIMDAAMRELVD
HHHHCCHHHHHHHHHHCCEEEECCCCHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHH
KGYRWFDLNMGCSVRKVVKTGCGAGMLRDVPNAVRVARAMTAVAGAGRVGFKFRLGWEQG
CCCEEEECCCCCHHHHHHHHCCCCCHHHHCHHHHHHHHHHHHHHCCCCCCEEEEECHHHH
AQVCMDLARRLEDAGAGWLTLHPRFARQGFSGTADWDALARLKEHVTIPVIASGDLFTAG
HHHHHHHHHHHHHCCCCEEEECHHHHHCCCCCCCCHHHHHHHHHCCEEEEEECCCEEECC
DAVRCMRASGVDGVMFARGALANPAVFEDFSAMMSGGEQPQPDPERTVRIIRRHVALARA
HHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
LCAEKTALFKMRTFVPRYVRQFPGARALRNHLASCLTWEGLDHLLDTFFERWRAQGAPML
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DDGACRVQTAPAGDDQCM
CCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]