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The map label for this gene is eno

Identifier: 78355342

GI number: 78355342

Start: 284884

End: 286179

Strand: Direct

Name: eno

Synonym: Dde_0295

Alternate gene names: 78355342

Gene position: 284884-286179 (Clockwise)

Preceding gene: 78355341

Following gene: 78355343

Centisome position: 7.64

GC content: 58.95

Gene sequence:

>1296_bases
ATGAGCACCATCGTTTCCGTCTGGGCCAGAGAAATCCTCGATTCCCGCGGCAATCCCACTGTGGAAGTGGAAGTTTCGCT
TGAATCCGGCCATTCCGGCCGCGCCGCCGTGCCTTCCGGCGCTTCCACCGGCAGCCGCGAGGCTCTGGAACTGCGTGACG
GCGACAAGGGCCGTTACCTTGGCAAAGGCGTTGAAAAAGCCGTGGACAACGTGATGGGCGAAATAGCCGAGAACATCGTC
GGCATGGACGCTCTGCGTCAGGTCGCCGTGGACAACGCGCTGATCGACCTTGACGGTACCGACAACAAGGACCGGCTGGG
CGCCAACGCCATGCTGGGCGTATCGCTGGCCACCGCCCGCGCCGCGGCCAACTTCATCGGCATGCCCCTGTATCAGTACC
TCGGCGGCATCAATTCCAAAGTACTGCCCGTGCCCATGATGAACATCATCAACGGCGGCGAGCATGCCCCCAACAACCTT
GATATTCAGGAATTCATGATCATGCCCGTGGGAGCGCCCACCTTCAGTGAAGCGCTGCGCATGGGGGCTGAAATTTTCCA
CAACCTCAAGGCGCTGCTGGCTGCCGACGGTCACGTGACCAGCGTGGGCGACGAAGGCGGCTTTGCTCCCAACCTGAAAA
GCCACGACGAGGCTTTCCGGTACATCATGCGCGCCATCGAGACCGCAGGCTATATTCCCGGGTCGGAAGTGGCACTGGCC
ATCGACGCCGCGGCTTCCGAATTTTACAAGGACGGCAAGTATCACCTCAAGGGCGAGAACAAAGTGCTCTCCTCTTCCGA
GATGATTGACTGGCTTATGGATTTCACCCAGCGTTACCCGCTTATTTCCATCGAAGACGGCATGGCCGAAGGCGACTGGG
AAGGCTGGCAGGAAATGACCGTCAAAATGGGTGACAGCGTGCAGATTGTGGGTGACGACGTTTTCGTGACCAACCCCGAC
ATTCTGGCTCAGGGCATCGATGAGGGCGTGGCCAACTCCATTCTTATCAAGCTGAACCAGATAGGTACCCTCACCGAGAC
GCTGGACACCGTGGAAATGGCCAAGGGTGCCGGTTATACCACGGTTATTTCGCACCGTTCCGGAGAAACAGAAGACCATT
TCATCGCCGACCTTGCCGTGGCACTGAACGCAGGGCAGATCAAGACCGGCTCGCTGTGCCGCAGCGACCGTCTGGCCAAA
TACAACCAGCTGCTGCGCATCGAGGAAGATCTGGATGACGAGGGCATCTACTTCGGCCCTTACATGGCTTCGCATTTCGG
GCTGGGCGAAGAGTAA

Upstream 100 bases:

>100_bases
ATGGAAAGCGGAATAAAGGGCTGATGCCTTTGCCGCAACCGCCCCAACAGGCGTAGCGGAGCAGTATTGACGCTCCGCCA
AGCTCAAGGAGAACAGCAGT

Downstream 100 bases:

>100_bases
ACCGCGCTGCTTGCATCCTGCCGCGGTTGGCTGTATGTAGCCGCCACGGAACCCGCCCGCGGGTGAGGATGCAAACAATG
TCATACAGGGATTTCACATA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MSTIVSVWAREILDSRGNPTVEVEVSLESGHSGRAAVPSGASTGSREALELRDGDKGRYLGKGVEKAVDNVMGEIAENIV
GMDALRQVAVDNALIDLDGTDNKDRLGANAMLGVSLATARAAANFIGMPLYQYLGGINSKVLPVPMMNIINGGEHAPNNL
DIQEFMIMPVGAPTFSEALRMGAEIFHNLKALLAADGHVTSVGDEGGFAPNLKSHDEAFRYIMRAIETAGYIPGSEVALA
IDAAASEFYKDGKYHLKGENKVLSSSEMIDWLMDFTQRYPLISIEDGMAEGDWEGWQEMTVKMGDSVQIVGDDVFVTNPD
ILAQGIDEGVANSILIKLNQIGTLTETLDTVEMAKGAGYTTVISHRSGETEDHFIADLAVALNAGQIKTGSLCRSDRLAK
YNQLLRIEEDLDDEGIYFGPYMASHFGLGEE

Sequences:

>Translated_431_residues
MSTIVSVWAREILDSRGNPTVEVEVSLESGHSGRAAVPSGASTGSREALELRDGDKGRYLGKGVEKAVDNVMGEIAENIV
GMDALRQVAVDNALIDLDGTDNKDRLGANAMLGVSLATARAAANFIGMPLYQYLGGINSKVLPVPMMNIINGGEHAPNNL
DIQEFMIMPVGAPTFSEALRMGAEIFHNLKALLAADGHVTSVGDEGGFAPNLKSHDEAFRYIMRAIETAGYIPGSEVALA
IDAAASEFYKDGKYHLKGENKVLSSSEMIDWLMDFTQRYPLISIEDGMAEGDWEGWQEMTVKMGDSVQIVGDDVFVTNPD
ILAQGIDEGVANSILIKLNQIGTLTETLDTVEMAKGAGYTTVISHRSGETEDHFIADLAVALNAGQIKTGSLCRSDRLAK
YNQLLRIEEDLDDEGIYFGPYMASHFGLGEE
>Mature_430_residues
STIVSVWAREILDSRGNPTVEVEVSLESGHSGRAAVPSGASTGSREALELRDGDKGRYLGKGVEKAVDNVMGEIAENIVG
MDALRQVAVDNALIDLDGTDNKDRLGANAMLGVSLATARAAANFIGMPLYQYLGGINSKVLPVPMMNIINGGEHAPNNLD
IQEFMIMPVGAPTFSEALRMGAEIFHNLKALLAADGHVTSVGDEGGFAPNLKSHDEAFRYIMRAIETAGYIPGSEVALAI
DAAASEFYKDGKYHLKGENKVLSSSEMIDWLMDFTQRYPLISIEDGMAEGDWEGWQEMTVKMGDSVQIVGDDVFVTNPDI
LAQGIDEGVANSILIKLNQIGTLTETLDTVEMAKGAGYTTVISHRSGETEDHFIADLAVALNAGQIKTGSLCRSDRLAKY
NQLLRIEEDLDDEGIYFGPYMASHFGLGEE

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=429, Percent_Identity=50.3496503496504, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI301897477, Length=426, Percent_Identity=50.4694835680751, Blast_Score=415, Evalue=1e-116,
Organism=Homo sapiens, GI301897469, Length=426, Percent_Identity=50.4694835680751, Blast_Score=415, Evalue=1e-116,
Organism=Homo sapiens, GI4503571, Length=429, Percent_Identity=49.6503496503497, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI301897479, Length=424, Percent_Identity=46.9339622641509, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI169201331, Length=341, Percent_Identity=25.5131964809384, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI169201757, Length=341, Percent_Identity=25.5131964809384, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI239744207, Length=341, Percent_Identity=25.5131964809384, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1789141, Length=424, Percent_Identity=62.2641509433962, Blast_Score=516, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI17536383, Length=429, Percent_Identity=50.8158508158508, Blast_Score=416, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI71995829, Length=429, Percent_Identity=50.8158508158508, Blast_Score=416, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32563855, Length=207, Percent_Identity=45.4106280193237, Blast_Score=187, Evalue=9e-48,
Organism=Saccharomyces cerevisiae, GI6321693, Length=431, Percent_Identity=50.3480278422274, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=49.6535796766744, Blast_Score=393, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=49.4226327944573, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=49.4226327944573, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321968, Length=431, Percent_Identity=50.3480278422274, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580918, Length=422, Percent_Identity=50, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580916, Length=422, Percent_Identity=50, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580920, Length=422, Percent_Identity=50, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580914, Length=422, Percent_Identity=50, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI281360527, Length=422, Percent_Identity=50, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI17137654, Length=422, Percent_Identity=50, Blast_Score=393, Evalue=1e-109,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_DESDG (Q316Q0)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_386791.1
- HSSP:   P0A6P9
- ProteinModelPortal:   Q316Q0
- SMR:   Q316Q0
- STRING:   Q316Q0
- GeneID:   3755116
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_0295
- NMPDR:   fig|207559.3.peg.1085
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- BioCyc:   DDES207559:DDE_0295-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46385; Mature: 46254

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 337-337 BINDING 155-155 BINDING 164-164 BINDING 285-285 BINDING 312-312 BINDING 337-337 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIVSVWAREILDSRGNPTVEVEVSLESGHSGRAAVPSGASTGSREALELRDGDKGRYL
CCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCHH
GKGVEKAVDNVMGEIAENIVGMDALRQVAVDNALIDLDGTDNKDRLGANAMLGVSLATAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHCCCCHHHHHHHHHHH
AAANFIGMPLYQYLGGINSKVLPVPMMNIINGGEHAPNNLDIQEFMIMPVGAPTFSEALR
HHHHHHCCHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHEEEEECCCCHHHHHHH
MGAEIFHNLKALLAADGHVTSVGDEGGFAPNLKSHDEAFRYIMRAIETAGYIPGSEVALA
HHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
IDAAASEFYKDGKYHLKGENKVLSSSEMIDWLMDFTQRYPLISIEDGMAEGDWEGWQEMT
EEHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHH
VKMGDSVQIVGDDVFVTNPDILAQGIDEGVANSILIKLNQIGTLTETLDTVEMAKGAGYT
HHCCCEEEEEECCEEEECHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHHCCCCCE
TVISHRSGETEDHFIADLAVALNAGQIKTGSLCRSDRLAKYNQLLRIEEDLDDEGIYFGP
EEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECC
YMASHFGLGEE
HHHHHCCCCCC
>Mature Secondary Structure 
STIVSVWAREILDSRGNPTVEVEVSLESGHSGRAAVPSGASTGSREALELRDGDKGRYL
CHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCHH
GKGVEKAVDNVMGEIAENIVGMDALRQVAVDNALIDLDGTDNKDRLGANAMLGVSLATAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHCCCCHHHHHHHHHHH
AAANFIGMPLYQYLGGINSKVLPVPMMNIINGGEHAPNNLDIQEFMIMPVGAPTFSEALR
HHHHHHCCHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHEEEEECCCCHHHHHHH
MGAEIFHNLKALLAADGHVTSVGDEGGFAPNLKSHDEAFRYIMRAIETAGYIPGSEVALA
HHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
IDAAASEFYKDGKYHLKGENKVLSSSEMIDWLMDFTQRYPLISIEDGMAEGDWEGWQEMT
EEHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHH
VKMGDSVQIVGDDVFVTNPDILAQGIDEGVANSILIKLNQIGTLTETLDTVEMAKGAGYT
HHCCCEEEEEECCEEEECHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHHCCCCCE
TVISHRSGETEDHFIADLAVALNAGQIKTGSLCRSDRLAKYNQLLRIEEDLDDEGIYFGP
EEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECC
YMASHFGLGEE
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA