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The map label for this gene is folD

Identifier: 78355343

GI number: 78355343

Start: 286436

End: 287296

Strand: Direct

Name: folD

Synonym: Dde_0296

Alternate gene names: 78355343

Gene position: 286436-287296 (Clockwise)

Preceding gene: 78355342

Following gene: 78355344

Centisome position: 7.68

GC content: 61.32

Gene sequence:

>861_bases
ATGATTCTGCTGGACGGTAAAGCCACCGCCGCGGCTGTGCGCGAAGAATTGAAAAAGGATGTGGACGCCCTGAAAGACCG
CGCCGGAAGAGCGCCCGGGCTGGCTGTTATTCTGGTGGGCGACGACCCCGCATCGCAGGTGTACGTGCGCAACAAAGAAC
GTGCCTGCGCCGACGCCGGAATCCGTTCCGAAGCGTTCCGCATCTCTGCACAGACCACGCAGCAGGAGCTTGAGGAACGC
ATTGCCGCCCTCAACGCCCGTGAAGACATAGACGGCATTCTGCTGCAGCTGCCTCTGCCCGCCGGACTGGACAGCCAGCG
CTGTCTTGAACTTATCGACCCCGCCAAGGATGTGGACGGCTTTCATCCTGTCAACATGGGCAAACTGACGCTGGGGCTTC
CCGGCTTCCGGCCCTGTACGCCGGCAGGCGTGATGACACTGCTGGAACGCTACAACCTGTCGCCGGCGGGCAAAAAAGCC
GTTGTGCTCGGCCGCTCGAATATTGTGGGCAAGCCTTTGGCGCTCATGCTGGGCGCTTCGGGTCCTTTTGCCAATGCCAC
TGTCACCGTGTGTCATTCGCGCACTCCCGACCTTGCGCAGCAGTGCCGCGAAGCGGATTTTCTGTTTGTGGCCATCGGCA
GGGCCAACTTTGTCACCGCAGACATGGTGAAACCCGGTGCCGTGGTGGTGGACGTGGGCATCAACCGGACGGAAAACGGC
CTTGCGGGAGATGTTGATTTCGGCCCTGTAAGCAAGGTGGCCTCTGCCATTACGCCTGTGCCGGGCGGAATAGGCCCCAT
GACCATCGCGCAGCTGCTGGTGAATACCGTGGCTTCGTGGAAGAAACGCTGCGGCGTGTAA

Upstream 100 bases:

>100_bases
GCTGTCTGCTCCGGCCGGTTTTTTTGTTGCGCCGGTCTGGCGGCGCGCGACAGGCTGCATAACCATACTATGTGCTGCAT
ACACTGCAAGGAGTACGCCC

Downstream 100 bases:

>100_bases
CTGCGGGGTAACCGCATGCCGTCCGCCGCTGTCTCTGCCCCGGCTGCAGGAACACCGCCGTGGGGGCTGCCTTTCGCCGC
GCATGGCGGACGGCCGCGCG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MILLDGKATAAAVREELKKDVDALKDRAGRAPGLAVILVGDDPASQVYVRNKERACADAGIRSEAFRISAQTTQQELEER
IAALNAREDIDGILLQLPLPAGLDSQRCLELIDPAKDVDGFHPVNMGKLTLGLPGFRPCTPAGVMTLLERYNLSPAGKKA
VVLGRSNIVGKPLALMLGASGPFANATVTVCHSRTPDLAQQCREADFLFVAIGRANFVTADMVKPGAVVVDVGINRTENG
LAGDVDFGPVSKVASAITPVPGGIGPMTIAQLLVNTVASWKKRCGV

Sequences:

>Translated_286_residues
MILLDGKATAAAVREELKKDVDALKDRAGRAPGLAVILVGDDPASQVYVRNKERACADAGIRSEAFRISAQTTQQELEER
IAALNAREDIDGILLQLPLPAGLDSQRCLELIDPAKDVDGFHPVNMGKLTLGLPGFRPCTPAGVMTLLERYNLSPAGKKA
VVLGRSNIVGKPLALMLGASGPFANATVTVCHSRTPDLAQQCREADFLFVAIGRANFVTADMVKPGAVVVDVGINRTENG
LAGDVDFGPVSKVASAITPVPGGIGPMTIAQLLVNTVASWKKRCGV
>Mature_286_residues
MILLDGKATAAAVREELKKDVDALKDRAGRAPGLAVILVGDDPASQVYVRNKERACADAGIRSEAFRISAQTTQQELEER
IAALNAREDIDGILLQLPLPAGLDSQRCLELIDPAKDVDGFHPVNMGKLTLGLPGFRPCTPAGVMTLLERYNLSPAGKKA
VVLGRSNIVGKPLALMLGASGPFANATVTVCHSRTPDLAQQCREADFLFVAIGRANFVTADMVKPGAVVVDVGINRTENG
LAGDVDFGPVSKVASAITPVPGGIGPMTIAQLLVNTVASWKKRCGV

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI94721354, Length=293, Percent_Identity=42.320819112628, Blast_Score=224, Evalue=9e-59,
Organism=Homo sapiens, GI222418558, Length=293, Percent_Identity=42.6621160409556, Blast_Score=217, Evalue=9e-57,
Organism=Homo sapiens, GI222136639, Length=292, Percent_Identity=39.3835616438356, Blast_Score=198, Evalue=5e-51,
Organism=Escherichia coli, GI1786741, Length=278, Percent_Identity=51.0791366906475, Blast_Score=274, Evalue=5e-75,
Organism=Caenorhabditis elegans, GI17568735, Length=261, Percent_Identity=42.1455938697318, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6319558, Length=291, Percent_Identity=42.9553264604811, Blast_Score=221, Evalue=9e-59,
Organism=Saccharomyces cerevisiae, GI6321643, Length=299, Percent_Identity=40.133779264214, Blast_Score=210, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6322933, Length=309, Percent_Identity=24.5954692556634, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17136816, Length=293, Percent_Identity=45.7337883959044, Blast_Score=254, Evalue=5e-68,
Organism=Drosophila melanogaster, GI17136818, Length=293, Percent_Identity=45.7337883959044, Blast_Score=254, Evalue=6e-68,
Organism=Drosophila melanogaster, GI62472483, Length=288, Percent_Identity=44.7916666666667, Blast_Score=225, Evalue=2e-59,
Organism=Drosophila melanogaster, GI45551871, Length=288, Percent_Identity=44.7916666666667, Blast_Score=225, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24645718, Length=288, Percent_Identity=44.7916666666667, Blast_Score=225, Evalue=3e-59,
Organism=Drosophila melanogaster, GI17137370, Length=288, Percent_Identity=44.7916666666667, Blast_Score=225, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_DESDG (Q316P9)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_386792.1
- ProteinModelPortal:   Q316P9
- SMR:   Q316P9
- STRING:   Q316P9
- GeneID:   3755117
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_0296
- NMPDR:   fig|207559.3.peg.1086
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   DVVYETA
- ProtClustDB:   PRK14184
- BioCyc:   DDES207559:DDE_0296-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30071; Mature: 30071

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILLDGKATAAAVREELKKDVDALKDRAGRAPGLAVILVGDDPASQVYVRNKERACADAG
CEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEECCHHHHHHCC
IRSEAFRISAQTTQQELEERIAALNAREDIDGILLQLPLPAGLDSQRCLELIDPAKDVDG
CCCHHEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHCCHHHCCC
FHPVNMGKLTLGLPGFRPCTPAGVMTLLERYNLSPAGKKAVVLGRSNIVGKPLALMLGAS
CCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEEECC
GPFANATVTVCHSRTPDLAQQCREADFLFVAIGRANFVTADMVKPGAVVVDVGINRTENG
CCCCCCEEEEEECCCHHHHHHHHCCCEEEEEECCCCEEECCCCCCCEEEEEECCCCCCCC
LAGDVDFGPVSKVASAITPVPGGIGPMTIAQLLVNTVASWKKRCGV
CCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MILLDGKATAAAVREELKKDVDALKDRAGRAPGLAVILVGDDPASQVYVRNKERACADAG
CEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEECCHHHHHHCC
IRSEAFRISAQTTQQELEERIAALNAREDIDGILLQLPLPAGLDSQRCLELIDPAKDVDG
CCCHHEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHCCHHHCCC
FHPVNMGKLTLGLPGFRPCTPAGVMTLLERYNLSPAGKKAVVLGRSNIVGKPLALMLGAS
CCCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEEEECC
GPFANATVTVCHSRTPDLAQQCREADFLFVAIGRANFVTADMVKPGAVVVDVGINRTENG
CCCCCCEEEEEECCCHHHHHHHHCCCEEEEEECCCCEEECCCCCCCEEEEEECCCCCCCC
LAGDVDFGPVSKVASAITPVPGGIGPMTIAQLLVNTVASWKKRCGV
CCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA