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The map label for this gene is slt [H]

Identifier: 78222589

GI number: 78222589

Start: 1539404

End: 1541563

Strand: Reverse

Name: slt [H]

Synonym: Gmet_1377

Alternate gene names: 78222589

Gene position: 1541563-1539404 (Counterclockwise)

Preceding gene: 78222590

Following gene: 78222588

Centisome position: 38.56

GC content: 63.33

Gene sequence:

>2160_bases
ATGCTTAACCGCTTGCTCCTTGCCACGGCCCTGCTCCTCTGTCTGGCTGTCTCCGCCTCCTCCCAGAACCTTACCCCCAC
ACTCGACGAAAAACTCGCCAAGGCCGCCGTGCGGCTCAAGCTCAAGGACAACCGGGGGGCCCTGGACAACGCCACGGACG
CAGCCGACTCTCCGGAAAAGCACCTGATTGCCGGCGTCGCCGCATACCGGCTCGAAGAGTGGGTCAATGCAGCCGAACTG
CTCGGCAAGGCGGCAAAGGAGCTTCCCCTTGTGGCCGACTACCCGCTCTTCTGGGAGGCTGATGCCCTGTATCGCATCGC
ACGCCACGATGAAGCCCTTGAAACCGTGAAGAGGCTCCTCCATGAACGGCCCGAAAGCCCCCTCGTCCGCCGGGCACGGA
TGCTCCAGGCCGACATCCTCTTTGCCCGCGGCGACCTGAAGGAGGCCCAGGCCGTCTACATCCGGTTCGTGGAAAGCTAT
CCCTCGGGGAGGGATTCCCTCACCGCCATTTACCAGGCAGCCCGCTGCCGGGAAGGGCTCGGCGACAAGGCAAAGGCGGT
TCAGGAGCTGCGGAATCTCTGGCTCGCCTACCCTGCCTCGCCGGTGGCCGAAGACGCCGAGGAGGCTCTGCGCCAACTGG
AACGCCAGGGATTCCCGGCTGCGCCATACACCCCCGATGAGCTTTTCCGGAGAGCAAGTACCCTGTACAGCCTCGGCCGG
TTCCAGCAGGCCGTCAAAACCTTTGACGCAATCCCGCTGGCGAACCAGTCGGCCGACTTCATTGCCCGGGTCACGTTCAA
GAGCGGTCAGGCCCTTTACAAGGCCCGTCAACGGAAGGATGCCGAACGGACCTTTGCCCGGCTTCTGGAAAAGGAGCTGA
AACCGTCCCTGGCCGAGGAGACCCGGTTCTGGCATGCCAAAGCTCTTGAGGGGATCGGGAAAAGCGACGACGCCGTCAAT
GCCTATCTCAAGATAGCCGAGGCCTCGCCGCGGGGAGAGCTGGCCAACGAGGCGCTTCTGGAGGCAGCCTTCATCCGCAA
ATTCCAGGGACGGTATCAGGAAGAGCTTCCCCTGCTTGACCGGCTCCTTGTCGCTGCCACCGATGTGAAGCTCCGGCAAC
GGGCCACGTGGGAGGCGGCGTGGGCCCGCTACGGCACGAAGGACTTCCGGGGGGCAGCCGACGCATTCAAGGCCCTCCAA
ACCATCCCGGAATACCGGGAGCGGGCCCTCTACTGGCATGGCCGCGCCCTTGAAGGGGCGGGAGACAAGGAGGGGGCTGC
CGCTTCGCTAGCCCGCGTCCAGGAAGAATCTCCCCTCTCCTTTTACGGCCGCCGGGCCTGCACGGCGCTGAATACCATCG
AGGAAACCCCTTCCCTGGCGGCCGAGCCGGCTGCATTGTCCCTGCCGATGCCCGCCGGGTATGAGCGTATCAAGCTCCTC
ATTGCCGTGGGACTCCACGACGAAGCCCGGAAGGAGCTTGCATCCGTCAGGAAGAAAAACGGTCTGAAGCAGAAGTCTCT
CCTGGGAGTGGCCCGCCTCTATCTGGAAATGGAAGACTACAACTCGGCCGCAGCCATCTATCGGGACAATCTCCCCCGCC
GGATTGACAGGGATTCCCTCACCCAGTGGGGGCTCCTCTATCCAAAGGCCTTCCGTGAGGCGGTAACCCGTCAGGCCACC
GACCATGGGGTAGCGGAAGAGCTCGTCTACGGTGTCATCAGGGCGGAGAGCACCTTCTCGCCGACTGTCGTCTCCCCCGT
GGGGGCCGTGGGGCTCATGCAGCTCATGCCGGCCACCGCCAGGGCAATGGTGAACGGCAAGGGGGCCAACATCTCCACGC
GCCTCACCGATCCCGCCTTCAACATCAACCTCGGCGTGCGCCACCTGAAAGGGCTGCTCAACCAGTACCACGGCAACACA
GTGGCTGCCGTGGCAGCCTACAACGCCGGCTCCACCCCGGTGGACCGCTGGCGCCGCACCCTCCCCTACCAACGTGATGA
CGAGTTCATCGAAAACATCCCCTACTATGAGACACGGGAATACGTGAAGAAGGTCCTCGCCAATGCGGAGATCTACCGTG
CCCTCTACCGGGCAACTCTCGTAAAGACACCTGAATCCGCCATCCAGAAGGCGTATTCAGGCGTCCAGAATTCAAAATAG

Upstream 100 bases:

>100_bases
GTAGTATGGGTCAAACGCCGACACAACGCATCATGGCCGGCTTTCTGCTCACCGAATTGACTGCCCCCGTTCAAATCCAA
TCCACGAAGGTTCGATGCCC

Downstream 100 bases:

>100_bases
CGGGGCTCTTGATCTTGATTCTTTGCATCCCTCTCCGTCCTGAGCTTCCAAATTGACAGCATACTGATTGTCTGTATAAT
GCCCGCGCCATTGCCGCCCG

Product: lytic transglycosylase, catalytic

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 719; Mature: 719

Protein sequence:

>719_residues
MLNRLLLATALLLCLAVSASSQNLTPTLDEKLAKAAVRLKLKDNRGALDNATDAADSPEKHLIAGVAAYRLEEWVNAAEL
LGKAAKELPLVADYPLFWEADALYRIARHDEALETVKRLLHERPESPLVRRARMLQADILFARGDLKEAQAVYIRFVESY
PSGRDSLTAIYQAARCREGLGDKAKAVQELRNLWLAYPASPVAEDAEEALRQLERQGFPAAPYTPDELFRRASTLYSLGR
FQQAVKTFDAIPLANQSADFIARVTFKSGQALYKARQRKDAERTFARLLEKELKPSLAEETRFWHAKALEGIGKSDDAVN
AYLKIAEASPRGELANEALLEAAFIRKFQGRYQEELPLLDRLLVAATDVKLRQRATWEAAWARYGTKDFRGAADAFKALQ
TIPEYRERALYWHGRALEGAGDKEGAAASLARVQEESPLSFYGRRACTALNTIEETPSLAAEPAALSLPMPAGYERIKLL
IAVGLHDEARKELASVRKKNGLKQKSLLGVARLYLEMEDYNSAAAIYRDNLPRRIDRDSLTQWGLLYPKAFREAVTRQAT
DHGVAEELVYGVIRAESTFSPTVVSPVGAVGLMQLMPATARAMVNGKGANISTRLTDPAFNINLGVRHLKGLLNQYHGNT
VAAVAAYNAGSTPVDRWRRTLPYQRDDEFIENIPYYETREYVKKVLANAEIYRALYRATLVKTPESAIQKAYSGVQNSK

Sequences:

>Translated_719_residues
MLNRLLLATALLLCLAVSASSQNLTPTLDEKLAKAAVRLKLKDNRGALDNATDAADSPEKHLIAGVAAYRLEEWVNAAEL
LGKAAKELPLVADYPLFWEADALYRIARHDEALETVKRLLHERPESPLVRRARMLQADILFARGDLKEAQAVYIRFVESY
PSGRDSLTAIYQAARCREGLGDKAKAVQELRNLWLAYPASPVAEDAEEALRQLERQGFPAAPYTPDELFRRASTLYSLGR
FQQAVKTFDAIPLANQSADFIARVTFKSGQALYKARQRKDAERTFARLLEKELKPSLAEETRFWHAKALEGIGKSDDAVN
AYLKIAEASPRGELANEALLEAAFIRKFQGRYQEELPLLDRLLVAATDVKLRQRATWEAAWARYGTKDFRGAADAFKALQ
TIPEYRERALYWHGRALEGAGDKEGAAASLARVQEESPLSFYGRRACTALNTIEETPSLAAEPAALSLPMPAGYERIKLL
IAVGLHDEARKELASVRKKNGLKQKSLLGVARLYLEMEDYNSAAAIYRDNLPRRIDRDSLTQWGLLYPKAFREAVTRQAT
DHGVAEELVYGVIRAESTFSPTVVSPVGAVGLMQLMPATARAMVNGKGANISTRLTDPAFNINLGVRHLKGLLNQYHGNT
VAAVAAYNAGSTPVDRWRRTLPYQRDDEFIENIPYYETREYVKKVLANAEIYRALYRATLVKTPESAIQKAYSGVQNSK
>Mature_719_residues
MLNRLLLATALLLCLAVSASSQNLTPTLDEKLAKAAVRLKLKDNRGALDNATDAADSPEKHLIAGVAAYRLEEWVNAAEL
LGKAAKELPLVADYPLFWEADALYRIARHDEALETVKRLLHERPESPLVRRARMLQADILFARGDLKEAQAVYIRFVESY
PSGRDSLTAIYQAARCREGLGDKAKAVQELRNLWLAYPASPVAEDAEEALRQLERQGFPAAPYTPDELFRRASTLYSLGR
FQQAVKTFDAIPLANQSADFIARVTFKSGQALYKARQRKDAERTFARLLEKELKPSLAEETRFWHAKALEGIGKSDDAVN
AYLKIAEASPRGELANEALLEAAFIRKFQGRYQEELPLLDRLLVAATDVKLRQRATWEAAWARYGTKDFRGAADAFKALQ
TIPEYRERALYWHGRALEGAGDKEGAAASLARVQEESPLSFYGRRACTALNTIEETPSLAAEPAALSLPMPAGYERIKLL
IAVGLHDEARKELASVRKKNGLKQKSLLGVARLYLEMEDYNSAAAIYRDNLPRRIDRDSLTQWGLLYPKAFREAVTRQAT
DHGVAEELVYGVIRAESTFSPTVVSPVGAVGLMQLMPATARAMVNGKGANISTRLTDPAFNINLGVRHLKGLLNQYHGNT
VAAVAAYNAGSTPVDRWRRTLPYQRDDEFIENIPYYETREYVKKVLANAEIYRALYRATLVKTPESAIQKAYSGVQNSK

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=236, Percent_Identity=31.7796610169492, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 80084; Mature: 80084

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: PS50293 TPR_REGION ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNRLLLATALLLCLAVSASSQNLTPTLDEKLAKAAVRLKLKDNRGALDNATDAADSPEK
CCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCHH
HLIAGVAAYRLEEWVNAAELLGKAAKELPLVADYPLFWEADALYRIARHDEALETVKRLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCEECHHHHHHHHHHHHHHHHHHHHH
HERPESPLVRRARMLQADILFARGDLKEAQAVYIRFVESYPSGRDSLTAIYQAARCREGL
HHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
GDKAKAVQELRNLWLAYPASPVAEDAEEALRQLERQGFPAAPYTPDELFRRASTLYSLGR
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
FQQAVKTFDAIPLANQSADFIARVTFKSGQALYKARQRKDAERTFARLLEKELKPSLAEE
HHHHHHHHHCCCCCCCCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
TRFWHAKALEGIGKSDDAVNAYLKIAEASPRGELANEALLEAAFIRKFQGRYQEELPLLD
HHHHHHHHHHCCCCCCHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLLVAATDVKLRQRATWEAAWARYGTKDFRGAADAFKALQTIPEYRERALYWHGRALEGA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEECCC
GDKEGAAASLARVQEESPLSFYGRRACTALNTIEETPSLAAEPAALSLPMPAGYERIKLL
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHH
IAVGLHDEARKELASVRKKNGLKQKSLLGVARLYLEMEDYNSAAAIYRDNLPRRIDRDSL
HHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHH
TQWGLLYPKAFREAVTRQATDHGVAEELVYGVIRAESTFSPTVVSPVGAVGLMQLMPATA
HHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHCHHHHHHHHHHHHHHH
RAMVNGKGANISTRLTDPAFNINLGVRHLKGLLNQYHGNTVAAVAAYNAGSTPVDRWRRT
HHHHCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHH
LPYQRDDEFIENIPYYETREYVKKVLANAEIYRALYRATLVKTPESAIQKAYSGVQNSK
CCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLNRLLLATALLLCLAVSASSQNLTPTLDEKLAKAAVRLKLKDNRGALDNATDAADSPEK
CCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCHH
HLIAGVAAYRLEEWVNAAELLGKAAKELPLVADYPLFWEADALYRIARHDEALETVKRLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCEECHHHHHHHHHHHHHHHHHHHHH
HERPESPLVRRARMLQADILFARGDLKEAQAVYIRFVESYPSGRDSLTAIYQAARCREGL
HHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
GDKAKAVQELRNLWLAYPASPVAEDAEEALRQLERQGFPAAPYTPDELFRRASTLYSLGR
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
FQQAVKTFDAIPLANQSADFIARVTFKSGQALYKARQRKDAERTFARLLEKELKPSLAEE
HHHHHHHHHCCCCCCCCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
TRFWHAKALEGIGKSDDAVNAYLKIAEASPRGELANEALLEAAFIRKFQGRYQEELPLLD
HHHHHHHHHHCCCCCCHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLLVAATDVKLRQRATWEAAWARYGTKDFRGAADAFKALQTIPEYRERALYWHGRALEGA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEECCC
GDKEGAAASLARVQEESPLSFYGRRACTALNTIEETPSLAAEPAALSLPMPAGYERIKLL
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHH
IAVGLHDEARKELASVRKKNGLKQKSLLGVARLYLEMEDYNSAAAIYRDNLPRRIDRDSL
HHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHH
TQWGLLYPKAFREAVTRQATDHGVAEELVYGVIRAESTFSPTVVSPVGAVGLMQLMPATA
HHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHCHHHHHHHHHHHHHHH
RAMVNGKGANISTRLTDPAFNINLGVRHLKGLLNQYHGNTVAAVAAYNAGSTPVDRWRRT
HHHHCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHH
LPYQRDDEFIENIPYYETREYVKKVLANAEIYRALYRATLVKTPESAIQKAYSGVQNSK
CCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]