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The map label for this gene is rlmB [H]

Identifier: 78222568

GI number: 78222568

Start: 1517972

End: 1518724

Strand: Direct

Name: rlmB [H]

Synonym: Gmet_1356

Alternate gene names: 78222568

Gene position: 1517972-1518724 (Clockwise)

Preceding gene: 78222567

Following gene: 78222569

Centisome position: 37.97

GC content: 60.16

Gene sequence:

>753_bases
GTGAAAGAAGAGATACTTTACGGGATCAACAGTGCCATGGAGGCCCTGCGCGGCAAGCGGAGGGCCTTTGAGCTTTTCGT
CTCCCGCGATGCCAGCGATCGCCGCATGGAGAAGCTCCTGAAGCTTGCCACAGAGAAGGGAGTCCCGGTCCGCAACCGTG
ACAAGCGCGATATCAGTCGTCTCTGTGGCACCGATCACCATCAGGGGGTGGCACTCAAGCTAGAGGGGTTTCCCTATACC
GGGTTGAGCGATATTATCGACGCCTGGCGCGAATCCGGGGAGAGGGGACTTATCCTCGCCCTCGACGGTGTGCAGGACCC
CCACAACCTGGGTGCCCTCATCCGGAGCGCCGCCTGTGCTGGTGCACACGGGGTCATCATCCCCAAGGATAGGGCCGCCA
GGGTGACGGCCACGGTGGAGAAGAGCGCCGCAGGTGCCGCTGAAACCATACCCGTTGCCCAGGTCACCAATCTGGCCCAA
GCCCTCGACGAGTTGAAGGAGGCTGGCTTCTGGATTTACGGCGCTGCCGACAGTGCCGCCTCGTCCCTCTATGATCAGGA
TCTGTCTGGAAATGTAGTTATTGTGATTGGCAGTGAAGGGGAGGGGGTGAGGCCCCTGGTGCGGAAGAAGTGTGATTTCC
TCGTTGCCATTCCGCTCAGGGGAGGCGTAAGTTCCCTCAACGCATCGGTTGCTGGGGGGGTAATTCTCTTCGAGGTCCTT
CGCCAGAGGCTGGCTTTTTCACCTCAGAACTGA

Upstream 100 bases:

>100_bases
GTCATCGGCCGCCCCATTGGTGATGCGCCGGACCCGGCCGCTGCCGCCGAGCTGATTCTCGGCGAGATTGTTGCCGGCTG
AGCCGGATGAAAGGCTACGA

Downstream 100 bases:

>100_bases
AAAAGGGTCTTCAGCAATGATGCTGAAGACCCTCTGATTTTGAATGGTGCCGGAGGTCGGAATCGAACCGACACGGGATT
GCTCCCGGCGGATTTTGAGT

Product: RNA methyltransferase TrmH, group 3

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MKEEILYGINSAMEALRGKRRAFELFVSRDASDRRMEKLLKLATEKGVPVRNRDKRDISRLCGTDHHQGVALKLEGFPYT
GLSDIIDAWRESGERGLILALDGVQDPHNLGALIRSAACAGAHGVIIPKDRAARVTATVEKSAAGAAETIPVAQVTNLAQ
ALDELKEAGFWIYGAADSAASSLYDQDLSGNVVIVIGSEGEGVRPLVRKKCDFLVAIPLRGGVSSLNASVAGGVILFEVL
RQRLAFSPQN

Sequences:

>Translated_250_residues
MKEEILYGINSAMEALRGKRRAFELFVSRDASDRRMEKLLKLATEKGVPVRNRDKRDISRLCGTDHHQGVALKLEGFPYT
GLSDIIDAWRESGERGLILALDGVQDPHNLGALIRSAACAGAHGVIIPKDRAARVTATVEKSAAGAAETIPVAQVTNLAQ
ALDELKEAGFWIYGAADSAASSLYDQDLSGNVVIVIGSEGEGVRPLVRKKCDFLVAIPLRGGVSSLNASVAGGVILFEVL
RQRLAFSPQN
>Mature_250_residues
MKEEILYGINSAMEALRGKRRAFELFVSRDASDRRMEKLLKLATEKGVPVRNRDKRDISRLCGTDHHQGVALKLEGFPYT
GLSDIIDAWRESGERGLILALDGVQDPHNLGALIRSAACAGAHGVIIPKDRAARVTATVEKSAAGAAETIPVAQVTNLAQ
ALDELKEAGFWIYGAADSAASSLYDQDLSGNVVIVIGSEGEGVRPLVRKKCDFLVAIPLRGGVSSLNASVAGGVILFEVL
RQRLAFSPQN

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=268, Percent_Identity=28.3582089552239, Blast_Score=105, Evalue=5e-23,
Organism=Escherichia coli, GI1790623, Length=245, Percent_Identity=41.6326530612245, Blast_Score=188, Evalue=3e-49,
Organism=Escherichia coli, GI1788935, Length=192, Percent_Identity=31.25, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1790083, Length=150, Percent_Identity=32, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17555908, Length=261, Percent_Identity=30.6513409961686, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324775, Length=294, Percent_Identity=27.5510204081633, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26819; Mature: 26819

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEEILYGINSAMEALRGKRRAFELFVSRDASDRRMEKLLKLATEKGVPVRNRDKRDISR
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHH
LCGTDHHQGVALKLEGFPYTGLSDIIDAWRESGERGLILALDGVQDPHNLGALIRSAACA
HHCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCC
GAHGVIIPKDRAARVTATVEKSAAGAAETIPVAQVTNLAQALDELKEAGFWIYGAADSAA
CCCCEEEECCCCCEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCHHHH
SSLYDQDLSGNVVIVIGSEGEGVRPLVRKKCDFLVAIPLRGGVSSLNASVAGGVILFEVL
HHHHHCCCCCCEEEEECCCCCCCCHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
RQRLAFSPQN
HHHHCCCCCC
>Mature Secondary Structure
MKEEILYGINSAMEALRGKRRAFELFVSRDASDRRMEKLLKLATEKGVPVRNRDKRDISR
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHH
LCGTDHHQGVALKLEGFPYTGLSDIIDAWRESGERGLILALDGVQDPHNLGALIRSAACA
HHCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCC
GAHGVIIPKDRAARVTATVEKSAAGAAETIPVAQVTNLAQALDELKEAGFWIYGAADSAA
CCCCEEEECCCCCEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCHHHH
SSLYDQDLSGNVVIVIGSEGEGVRPLVRKKCDFLVAIPLRGGVSSLNASVAGGVILFEVL
HHHHHCCCCCCEEEEECCCCCCCCHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
RQRLAFSPQN
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]