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The map label for this gene is aspS

Identifier: 78222569

GI number: 78222569

Start: 1519234

End: 1521018

Strand: Direct

Name: aspS

Synonym: Gmet_1357

Alternate gene names: 78222569

Gene position: 1519234-1521018 (Clockwise)

Preceding gene: 78222568

Following gene: 78222570

Centisome position: 38.01

GC content: 53.67

Gene sequence:

>1785_bases
ATGGAAGATATTCTGGGTGAATGGAAGAGAAGTCATTATTGCGGCAATCTGACGAAGGCCGATGTGGGGCGCGAGGTTAT
CCTCATGGGATGGATTCTGCGCCGCCGTGATCATGGGGGCCTTATTTTCGCCGATCTCCGTGACCGGGAGGGACTCGCCC
AGATCGTCTTTGACCCAGCGAAAAACACCGATGCCCATCATAAGGCCGAAGCGATCCGGAATGAATACGTCGTCGCCGTA
AAAGGGGAGGTGATTCCTCGTCCTGAAGGGACTGTCAACTCCGCCATGAAGACCGGCGAGGTCGAAGTTCTCGTTACGGA
GTGCAAGATTCTCAATCGCTCAAAGGCTCTACCTTTTACCCTTGACGACTATGTCGACGTCGCTGAGAACATCCGTCTCA
AGTACCGTTATCTGGACCTTCGGCGCCCGGTGCTCCAGCAGAACCTCATTCTCCGCTCGATGGTTGCCCAGATAACCCGT
CAATATCTCTCAGAAAACGGATTCCTTGAGATCGAAACTCCTTTCCTCACCAAATCAACTCCGGAAGGTGCCCGTGACTT
TCTCGTCCCGTCGCGGATAAATCAAGGGAACTTCTATGCGCTTCCCCAATCTCCCCAGATATTCAAACAGATTCTGATGA
TCTCCGGTTTTGACCGCTACTTCCAGATTGTTCGCTGCTTCCGTGACGAGGATCTGCGAGCTGACCGTCAGCCTGAGTTC
ACCCAGATCGACTGCGAGATGTCGTTTATCGATCGCGAGGACATCATTTCGGTCATGGAGGGGCTCATTGCGAAGATTTT
CACGGTTGCCAAGGGGGTGGATGTTCCCCTCCCCATGCCACGCATGACCTATGCTGAGGCCATTCGCCGCTTCGGCGTCG
ATAACCCCGACGTACGCTTTGGCCTGGAGCTTGTTGAGCTGACAGATATCGTCAAGGGAGCAGGTTTCAAGGTCTTTGCC
GATGTCGCAGCCTCCGGTGGAATCATCAAGGGACTGAACGCTAAAGGCTGCGCACGGTTTTCCCGCAAGGAGATCGACGA
TCTCACCGAGTTCGCCAAGATTTACGGTGCTAAAGGGCTTGCCTACGTCAAGATTGAAGAGGGCCAATGGCATTCCCCCA
TTGCCAAGTTTTTCTCTGCCGAAGAAATCGCTACAATGAACCAGGCGTTCGGCGCCGAAGATGGTGATCTTCTTCTCTTT
GTGGCGGACAAGCCGAAGGTGGTCAACGATTCTCTCGGTAAGCTCCGCAATCATCTTGCCGGAATTCTTGGGCTTGCGGA
CAAGAATACGTTCAGATTCGTCTGGATCACCGATTTCCCTCTCCTTGAGTGGGATGAGGATGAAAAGCGGTGGGCCGCGG
TCCATCATCCTTTCACCGCGCCCATGGATGAGGATCTGGAGAAAGTCGAATCGGATCCGGGAAGCTGCCGCGCGAAAGCA
TATGATCTGGTCCTCAATGGCAACGAAATCGGCGGGGGAAGTATCAGGATTCACCAGCAACATGTCCAATCCCTCATGTT
CAGCATGCTTGGACTTTCTGATGATGAGACGCGCGCAAAGTTCGGCTTCCTGCTGGATGCTCTCGAATGCGGTACCCCTC
CCCATGGCGGTATTGCCTTCGGCATGGATCGCCTCATCATGCTTCTTACGGGCAGCGATTCGATCCGTGACGTCATTGCC
TTCCCCAAAACTCAGAAGGGAGCTTGTCTCATGTCAGAAGCTCCTTCCCCTGTTGATGCAAAACAACTCAGGGAACTTGC
TCTCAAGGTGACCGTCAAGCAATGA

Upstream 100 bases:

>100_bases
CATCAACTTCCCGCTCCCTTACAGTTATGTTTTTCTTGAAGAGTTCTTTCATATTCAGTATGCTTACCCGATTTTAGCGA
CCTACGAGGAGGTTTTTCCG

Downstream 100 bases:

>100_bases
GAGCGGAAAAGTGAATGAAGACGAGAAACCTGGCAATGGTTTCGGTGCTGGCTCTTTGCCTTGCTGGTTGTGCTCAGGGG
CTGCCACGTTACCGTACCGA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 594; Mature: 594

Protein sequence:

>594_residues
MEDILGEWKRSHYCGNLTKADVGREVILMGWILRRRDHGGLIFADLRDREGLAQIVFDPAKNTDAHHKAEAIRNEYVVAV
KGEVIPRPEGTVNSAMKTGEVEVLVTECKILNRSKALPFTLDDYVDVAENIRLKYRYLDLRRPVLQQNLILRSMVAQITR
QYLSENGFLEIETPFLTKSTPEGARDFLVPSRINQGNFYALPQSPQIFKQILMISGFDRYFQIVRCFRDEDLRADRQPEF
TQIDCEMSFIDREDIISVMEGLIAKIFTVAKGVDVPLPMPRMTYAEAIRRFGVDNPDVRFGLELVELTDIVKGAGFKVFA
DVAASGGIIKGLNAKGCARFSRKEIDDLTEFAKIYGAKGLAYVKIEEGQWHSPIAKFFSAEEIATMNQAFGAEDGDLLLF
VADKPKVVNDSLGKLRNHLAGILGLADKNTFRFVWITDFPLLEWDEDEKRWAAVHHPFTAPMDEDLEKVESDPGSCRAKA
YDLVLNGNEIGGGSIRIHQQHVQSLMFSMLGLSDDETRAKFGFLLDALECGTPPHGGIAFGMDRLIMLLTGSDSIRDVIA
FPKTQKGACLMSEAPSPVDAKQLRELALKVTVKQ

Sequences:

>Translated_594_residues
MEDILGEWKRSHYCGNLTKADVGREVILMGWILRRRDHGGLIFADLRDREGLAQIVFDPAKNTDAHHKAEAIRNEYVVAV
KGEVIPRPEGTVNSAMKTGEVEVLVTECKILNRSKALPFTLDDYVDVAENIRLKYRYLDLRRPVLQQNLILRSMVAQITR
QYLSENGFLEIETPFLTKSTPEGARDFLVPSRINQGNFYALPQSPQIFKQILMISGFDRYFQIVRCFRDEDLRADRQPEF
TQIDCEMSFIDREDIISVMEGLIAKIFTVAKGVDVPLPMPRMTYAEAIRRFGVDNPDVRFGLELVELTDIVKGAGFKVFA
DVAASGGIIKGLNAKGCARFSRKEIDDLTEFAKIYGAKGLAYVKIEEGQWHSPIAKFFSAEEIATMNQAFGAEDGDLLLF
VADKPKVVNDSLGKLRNHLAGILGLADKNTFRFVWITDFPLLEWDEDEKRWAAVHHPFTAPMDEDLEKVESDPGSCRAKA
YDLVLNGNEIGGGSIRIHQQHVQSLMFSMLGLSDDETRAKFGFLLDALECGTPPHGGIAFGMDRLIMLLTGSDSIRDVIA
FPKTQKGACLMSEAPSPVDAKQLRELALKVTVKQ
>Mature_594_residues
MEDILGEWKRSHYCGNLTKADVGREVILMGWILRRRDHGGLIFADLRDREGLAQIVFDPAKNTDAHHKAEAIRNEYVVAV
KGEVIPRPEGTVNSAMKTGEVEVLVTECKILNRSKALPFTLDDYVDVAENIRLKYRYLDLRRPVLQQNLILRSMVAQITR
QYLSENGFLEIETPFLTKSTPEGARDFLVPSRINQGNFYALPQSPQIFKQILMISGFDRYFQIVRCFRDEDLRADRQPEF
TQIDCEMSFIDREDIISVMEGLIAKIFTVAKGVDVPLPMPRMTYAEAIRRFGVDNPDVRFGLELVELTDIVKGAGFKVFA
DVAASGGIIKGLNAKGCARFSRKEIDDLTEFAKIYGAKGLAYVKIEEGQWHSPIAKFFSAEEIATMNQAFGAEDGDLLLF
VADKPKVVNDSLGKLRNHLAGILGLADKNTFRFVWITDFPLLEWDEDEKRWAAVHHPFTAPMDEDLEKVESDPGSCRAKA
YDLVLNGNEIGGGSIRIHQQHVQSLMFSMLGLSDDETRAKFGFLLDALECGTPPHGGIAFGMDRLIMLLTGSDSIRDVIA
FPKTQKGACLMSEAPSPVDAKQLRELALKVTVKQ

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=594, Percent_Identity=42.4242424242424, Blast_Score=451, Evalue=1e-127,
Organism=Homo sapiens, GI5031815, Length=247, Percent_Identity=30.3643724696356, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI194272210, Length=247, Percent_Identity=30.3643724696356, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI45439306, Length=219, Percent_Identity=29.2237442922374, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788173, Length=590, Percent_Identity=51.3559322033898, Blast_Score=605, Evalue=1e-174,
Organism=Escherichia coli, GI1790571, Length=272, Percent_Identity=29.4117647058824, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1789256, Length=292, Percent_Identity=28.0821917808219, Blast_Score=90, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI32566633, Length=595, Percent_Identity=35.6302521008403, Blast_Score=328, Evalue=6e-90,
Organism=Caenorhabditis elegans, GI71994340, Length=262, Percent_Identity=26.3358778625954, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17535927, Length=262, Percent_Identity=26.3358778625954, Blast_Score=90, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17535925, Length=262, Percent_Identity=26.3358778625954, Blast_Score=90, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6325153, Length=633, Percent_Identity=33.175355450237, Blast_Score=290, Evalue=4e-79,
Organism=Saccharomyces cerevisiae, GI6323011, Length=268, Percent_Identity=27.2388059701493, Blast_Score=78, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6321807, Length=256, Percent_Identity=26.5625, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324256, Length=173, Percent_Identity=26.0115606936416, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320242, Length=176, Percent_Identity=26.1363636363636, Blast_Score=64, Evalue=1e-10,
Organism=Drosophila melanogaster, GI24584738, Length=599, Percent_Identity=39.5659432387312, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24640849, Length=285, Percent_Identity=28.7719298245614, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24640851, Length=285, Percent_Identity=28.7719298245614, Blast_Score=84, Evalue=4e-16,
Organism=Drosophila melanogaster, GI17136276, Length=164, Percent_Identity=31.0975609756098, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_GEOMG (Q39VY3)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_384316.1
- ProteinModelPortal:   Q39VY3
- SMR:   Q39VY3
- STRING:   Q39VY3
- GeneID:   3738693
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1357
- NMPDR:   fig|269799.3.peg.1198
- eggNOG:   COG0173
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- PhylomeDB:   Q39VY3
- ProtClustDB:   PRK00476
- BioCyc:   GMET269799:GMET_1357-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66613; Mature: 66613

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDILGEWKRSHYCGNLTKADVGREVILMGWILRRRDHGGLIFADLRDREGLAQIVFDPA
CCCHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEECCC
KNTDAHHKAEAIRNEYVVAVKGEVIPRPEGTVNSAMKTGEVEVLVTECKILNRSKALPFT
CCCCHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHCCCCEEEEEEEHHHHCCCCCCCEE
LDDYVDVAENIRLKYRYLDLRRPVLQQNLILRSMVAQITRQYLSENGFLEIETPFLTKST
HHHHHHHHHCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
PEGARDFLVPSRINQGNFYALPQSPQIFKQILMISGFDRYFQIVRCFRDEDLRADRQPEF
CCCHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCC
TQIDCEMSFIDREDIISVMEGLIAKIFTVAKGVDVPLPMPRMTYAEAIRRFGVDNPDVRF
EEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHH
GLELVELTDIVKGAGFKVFADVAASGGIIKGLNAKGCARFSRKEIDDLTEFAKIYGAKGL
HHHHHHHHHHHHCCCCEEEEEHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCE
AYVKIEEGQWHSPIAKFFSAEEIATMNQAFGAEDGDLLLFVADKPKVVNDSLGKLRNHLA
EEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
GILGLADKNTFRFVWITDFPLLEWDEDEKRWAAVHHPFTAPMDEDLEKVESDPGSCRAKA
HHHEECCCCEEEEEEEECCCCCCCCCCCHHEEHCCCCCCCCCHHHHHHHHCCCCCCCEEE
YDLVLNGNEIGGGSIRIHQQHVQSLMFSMLGLSDDETRAKFGFLLDALECGTPPHGGIAF
EEEEEECCEECCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHH
GMDRLIMLLTGSDSIRDVIAFPKTQKGACLMSEAPSPVDAKQLRELALKVTVKQ
CCCCEEEEEECCCHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHHHEEECC
>Mature Secondary Structure
MEDILGEWKRSHYCGNLTKADVGREVILMGWILRRRDHGGLIFADLRDREGLAQIVFDPA
CCCHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEECCC
KNTDAHHKAEAIRNEYVVAVKGEVIPRPEGTVNSAMKTGEVEVLVTECKILNRSKALPFT
CCCCHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHCCCCEEEEEEEHHHHCCCCCCCEE
LDDYVDVAENIRLKYRYLDLRRPVLQQNLILRSMVAQITRQYLSENGFLEIETPFLTKST
HHHHHHHHHCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
PEGARDFLVPSRINQGNFYALPQSPQIFKQILMISGFDRYFQIVRCFRDEDLRADRQPEF
CCCHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCC
TQIDCEMSFIDREDIISVMEGLIAKIFTVAKGVDVPLPMPRMTYAEAIRRFGVDNPDVRF
EEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHH
GLELVELTDIVKGAGFKVFADVAASGGIIKGLNAKGCARFSRKEIDDLTEFAKIYGAKGL
HHHHHHHHHHHHCCCCEEEEEHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCE
AYVKIEEGQWHSPIAKFFSAEEIATMNQAFGAEDGDLLLFVADKPKVVNDSLGKLRNHLA
EEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
GILGLADKNTFRFVWITDFPLLEWDEDEKRWAAVHHPFTAPMDEDLEKVESDPGSCRAKA
HHHEECCCCEEEEEEEECCCCCCCCCCCHHEEHCCCCCCCCCHHHHHHHHCCCCCCCEEE
YDLVLNGNEIGGGSIRIHQQHVQSLMFSMLGLSDDETRAKFGFLLDALECGTPPHGGIAF
EEEEEECCEECCCEEEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHH
GMDRLIMLLTGSDSIRDVIAFPKTQKGACLMSEAPSPVDAKQLRELALKVTVKQ
CCCCEEEEEECCCHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA