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The map label for this gene is ptsH [H]

Identifier: 78222502

GI number: 78222502

Start: 1447823

End: 1448089

Strand: Direct

Name: ptsH [H]

Synonym: Gmet_1288

Alternate gene names: 78222502

Gene position: 1447823-1448089 (Clockwise)

Preceding gene: 78222501

Following gene: 78222503

Centisome position: 36.22

GC content: 55.06

Gene sequence:

>267_bases
ATGCTCGACCAGGAATTCACCATCATCAACAAACTCGGTCTCCACGCACGGGCCTCGGCCCTTTTTGTCAAGACGGCAAG
CCGTTTTCGCTCTGACATAAAGATTGTGCGAGAGGGGATCGAGGTGAACGGCAAGAGCATCATGGGGATCATGATGCTGG
CCGCGGCCAAGGGAACACTTATCAGGGTGAAAATTGAGGGGGAGGACGAGGCGGAAGCAATGGCCGCCCTCGGAGAGCTT
ATAACCAATGGATTCGGCGAGGAATGA

Upstream 100 bases:

>100_bases
AGCTGTTGCCGAACTGGCCACGTCCCTCAAGGAGTGCGGCCGCGAGAGTATCGCCGTGGCCGGAGATTATCTGAAATAAC
CGGGAACTACAGGGACTGAA

Downstream 100 bases:

>100_bases
AACGAGGATTTTTCGCGGAATAGAAGCCTCGGCCGGCATCGCCATCGGCACGGCACGCTTGACCGACCGCAGTCGCGTGG
CGGTCACCGAGGTTTCCATC

Product: HPrNtr

Products: fructose-6-phosphate [Cytoplasm]; pyruvate; fructose-1-phosphate [Cytoplasm] [C]

Alternate protein names: Histidine-containing protein [H]

Number of amino acids: Translated: 88; Mature: 88

Protein sequence:

>88_residues
MLDQEFTIINKLGLHARASALFVKTASRFRSDIKIVREGIEVNGKSIMGIMMLAAAKGTLIRVKIEGEDEAEAMAALGEL
ITNGFGEE

Sequences:

>Translated_88_residues
MLDQEFTIINKLGLHARASALFVKTASRFRSDIKIVREGIEVNGKSIMGIMMLAAAKGTLIRVKIEGEDEAEAMAALGEL
ITNGFGEE
>Mature_88_residues
MLDQEFTIINKLGLHARASALFVKTASRFRSDIKIVREGIEVNGKSIMGIMMLAAAKGTLIRVKIEGEDEAEAMAALGEL
ITNGFGEE

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1925

COG function: function code G; Phosphotransferase system, HPr-related proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HPr domain [H]

Homologues:

Organism=Escherichia coli, GI1788494, Length=90, Percent_Identity=36.6666666666667, Blast_Score=64, Evalue=2e-12,
Organism=Escherichia coli, GI1788755, Length=81, Percent_Identity=38.2716049382716, Blast_Score=60, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114 [H]

Pfam domain/function: PF00381 PTS-HPr [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 9538; Mature: 9538

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS00369 PTS_HPR_HIS ; PS00589 PTS_HPR_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDQEFTIINKLGLHARASALFVKTASRFRSDIKIVREGIEVNGKSIMGIMMLAAAKGTL
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEE
IRVKIEGEDEAEAMAALGELITNGFGEE
EEEEECCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLDQEFTIINKLGLHARASALFVKTASRFRSDIKIVREGIEVNGKSIMGIMMLAAAKGTL
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEE
IRVKIEGEDEAEAMAALGELITNGFGEE
EEEEECCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Fructose [Periplasm]; phosphoenolpyruvate [C]

Specific reaction: phosphoenolpyruvate + fructose [Periplasm] = fructose-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + fructose [Periplasm] = fructose-1-phosphate [Cytoplasm] + pyruvate [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]