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The map label for this gene is manX [H]

Identifier: 78222501

GI number: 78222501

Start: 1447409

End: 1447801

Strand: Direct

Name: manX [H]

Synonym: Gmet_1287

Alternate gene names: 78222501

Gene position: 1447409-1447801 (Clockwise)

Preceding gene: 78222500

Following gene: 78222502

Centisome position: 36.21

GC content: 58.52

Gene sequence:

>393_bases
ATGATTGGATTGGTGCTCGTTACCCATGCCGGCCTTGCTGAGGAACTCCTCAGGGGGGCCGAGATGATCGTAGGACCCAT
TGAGTCCGCCGAAACCATCGGAATCCGGCCGGGAGATCAGGCTGATGCGATCATGGAGCGCATTGCAGAGGCCGTGAAAC
GTGTTTCCGTGGACGGTGCGCTTATCATGACGGACATGTTTGGCGGGACGCCGTCGAATATGAGTCTTTCCTTCCTCGAG
GCCGACCGCATCGAGGTGTTGACCGGCGTGAATCTGCCGATGGTCATCAAGTTTGCTGCCGAGCGGGACCGGATAGCTGT
TGCCGAACTGGCCACGTCCCTCAAGGAGTGCGGCCGCGAGAGTATCGCCGTGGCCGGAGATTATCTGAAATAA

Upstream 100 bases:

>100_bases
CACCGTTCCGTCGCTCTTGTCGAAGAGCTCCGCCGATTCTTCGACCGCGCAGGCATTGCGGTGAAGGTGAGCCACAGGGA
TATGGAAAAGGGATGAAAAG

Downstream 100 bases:

>100_bases
CCGGGAACTACAGGGACTGAAATGCTCGACCAGGAATTCACCATCATCAACAAACTCGGTCTCCACGCACGGGCCTCGGC
CCTTTTTGTCAAGACGGCAA

Product: PTS system fructose subfamily IIA component

Products: NA

Alternate protein names: EIIAB-Man; Mannose-specific phosphotransferase enzyme IIA component; EIII-Man; PTS system mannose-specific EIIA component; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component [H]

Number of amino acids: Translated: 130; Mature: 130

Protein sequence:

>130_residues
MIGLVLVTHAGLAEELLRGAEMIVGPIESAETIGIRPGDQADAIMERIAEAVKRVSVDGALIMTDMFGGTPSNMSLSFLE
ADRIEVLTGVNLPMVIKFAAERDRIAVAELATSLKECGRESIAVAGDYLK

Sequences:

>Translated_130_residues
MIGLVLVTHAGLAEELLRGAEMIVGPIESAETIGIRPGDQADAIMERIAEAVKRVSVDGALIMTDMFGGTPSNMSLSFLE
ADRIEVLTGVNLPMVIKFAAERDRIAVAELATSLKECGRESIAVAGDYLK
>Mature_130_residues
MIGLVLVTHAGLAEELLRGAEMIVGPIESAETIGIRPGDQADAIMERIAEAVKRVSVDGALIMTDMFGGTPSNMSLSFLE
ADRIEVLTGVNLPMVIKFAAERDRIAVAELATSLKECGRESIAVAGDYLK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=124, Percent_Identity=34.6774193548387, Blast_Score=70, Evalue=4e-14,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR013789
- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03610 EIIA-man; PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 13801; Mature: 13801

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGLVLVTHAGLAEELLRGAEMIVGPIESAETIGIRPGDQADAIMERIAEAVKRVSVDGA
CEEEEEEECCCHHHHHHCCHHHEECCCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCE
LIMTDMFGGTPSNMSLSFLEADRIEVLTGVNLPMVIKFAAERDRIAVAELATSLKECGRE
EEEEECCCCCCCCCEEEEECCCHHHEEECCCCCEEEEEECCCCCHHHHHHHHHHHHCCCC
SIAVAGDYLK
CEECCCCCCC
>Mature Secondary Structure
MIGLVLVTHAGLAEELLRGAEMIVGPIESAETIGIRPGDQADAIMERIAEAVKRVSVDGA
CEEEEEEECCCHHHHHHCCHHHEECCCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCE
LIMTDMFGGTPSNMSLSFLEADRIEVLTGVNLPMVIKFAAERDRIAVAELATSLKECGRE
EEEEECCCCCCCCCEEEEECCCHHHEEECCCCCEEEEEECCCCCHHHHHHHHHHHHCCCC
SIAVAGDYLK
CEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]