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The map label for this gene is leuC

Identifier: 78222482

GI number: 78222482

Start: 1428796

End: 1430079

Strand: Direct

Name: leuC

Synonym: Gmet_1268

Alternate gene names: 78222482

Gene position: 1428796-1430079 (Clockwise)

Preceding gene: 78222479

Following gene: 78222483

Centisome position: 35.74

GC content: 59.58

Gene sequence:

>1284_bases
ATGGGGAAGACTACAGCGGAAAAAATTTTTGCAAGCCACCTGGTCGATGAGCCGTTTTCCGGCACCAAGGTGCTCCGGCT
CGACGTGGTGATGTGCCACGAAATTACCACTCCCATAGCCATTGCCGACCTGATGGCGCGAGGTAAAGACCGGGTTTTTG
ACCCCACGAAGATCAAGGCGGTGATTGATCACGTCACGCCGAGCAAGGACTCGAAGACCGCCACCCAGGCGAAAATGTTG
CGCGACTGGGCACGGCGACACGCCATCAAGGACTTCTTCGATGTGGGGCATAACGGCGTGTGTCACGCACTCTTCCCCGA
GAAGGGGTACATCCGCCCCGGTTACACAGTCATCATGGGCGATTCCCATACCTGCACCCATGGCGCCTTCGGTGCCTTTG
CCGCCGGTGTCGGCACTACCGACCTGGAGGTCGGTATTCTCAAAGGGGTCTGCGCTTTCCGTGAGCCCAAGAGCATCCGG
ATCAACCTGAACGGCACCCTTCCCAAGGGCGTTTATGCAAAGGATGTCATCCTCTACGTCATCGGCCAGCTCGGCGTCAA
CGGCGCTACTGACCGGGTCATGGAGTTCCGGGGACCGGTGGTGGACGCCATGACCATGGAGTCGCGGATGACACTTTGCA
ACATGGCCATCGAGGCGGGAGGCACCTCCGGCATCTGCATGCCCGACATGGTGACGGTGGAATACCTCTGGCCCTTCATT
GAGAACGAGTACCCGTCCAAAGAGGCTGCCCTCGCCGAGTTCAGCACATGGCGCTCCGACGACGACGCTGTCTACGAGCG
GGTGCTCGACTTTGACGTTTCTGTCCTCGAGCCGATCGTCACCTTCGGCTACAAGCCCGATCAGGTGAAGCCGATCAGCG
AAATTGCCGGTTCTCCTGTTGATCAAGTCTATCTGGGCTCCTGCACTAACGGCCGCCTTGAAGATCTGCGGATTGCTGCC
CGGATTCTCAAAGGGAAAAAGATTGCCCCGTCGGTGCGAGGGATTCTCTCCCCGGCCACGCCGAAGATTTACAAGGACGC
CATGGCCGAGGGGCTTATCGACATTTTCATGGAGGCGGGTTTCTGCGTCACGAATCCCACCTGCGGTGCCTGCCTTGGCA
TGAGCAACGGCGTCTTGGCTGAAGGGGAGGTCTGTGCATCCACCACGAACCGCAACTTCATGGGGCGGATGGGGAAGGGG
GGGATGGTCCACCTCATGTCCCCGGCCACGAGCGCAGCAACGGCCATCGAAGGGGTGATCGCCGATCCCCGGAAGTACCT
GTAA

Upstream 100 bases:

>100_bases
AAATGTTAAGCCGGACCGAACAAATATTACGTTGATTTATTAGGGGGCTTTGTTTAGAGTGGCCACCATACTTTCATTAC
ACTGCGAAAGGGTAGTTCAT

Downstream 100 bases:

>100_bases
TCATGCAAATGGATGGGACGGTAGCCCTTTTGGATACTGGCCACCTGTGACCGACTTTTTCAAGGAGAATGCACCATGAA
ACAATTTGGCGGCCCCGTAC

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MGKTTAEKIFASHLVDEPFSGTKVLRLDVVMCHEITTPIAIADLMARGKDRVFDPTKIKAVIDHVTPSKDSKTATQAKML
RDWARRHAIKDFFDVGHNGVCHALFPEKGYIRPGYTVIMGDSHTCTHGAFGAFAAGVGTTDLEVGILKGVCAFREPKSIR
INLNGTLPKGVYAKDVILYVIGQLGVNGATDRVMEFRGPVVDAMTMESRMTLCNMAIEAGGTSGICMPDMVTVEYLWPFI
ENEYPSKEAALAEFSTWRSDDDAVYERVLDFDVSVLEPIVTFGYKPDQVKPISEIAGSPVDQVYLGSCTNGRLEDLRIAA
RILKGKKIAPSVRGILSPATPKIYKDAMAEGLIDIFMEAGFCVTNPTCGACLGMSNGVLAEGEVCASTTNRNFMGRMGKG
GMVHLMSPATSAATAIEGVIADPRKYL

Sequences:

>Translated_427_residues
MGKTTAEKIFASHLVDEPFSGTKVLRLDVVMCHEITTPIAIADLMARGKDRVFDPTKIKAVIDHVTPSKDSKTATQAKML
RDWARRHAIKDFFDVGHNGVCHALFPEKGYIRPGYTVIMGDSHTCTHGAFGAFAAGVGTTDLEVGILKGVCAFREPKSIR
INLNGTLPKGVYAKDVILYVIGQLGVNGATDRVMEFRGPVVDAMTMESRMTLCNMAIEAGGTSGICMPDMVTVEYLWPFI
ENEYPSKEAALAEFSTWRSDDDAVYERVLDFDVSVLEPIVTFGYKPDQVKPISEIAGSPVDQVYLGSCTNGRLEDLRIAA
RILKGKKIAPSVRGILSPATPKIYKDAMAEGLIDIFMEAGFCVTNPTCGACLGMSNGVLAEGEVCASTTNRNFMGRMGKG
GMVHLMSPATSAATAIEGVIADPRKYL
>Mature_426_residues
GKTTAEKIFASHLVDEPFSGTKVLRLDVVMCHEITTPIAIADLMARGKDRVFDPTKIKAVIDHVTPSKDSKTATQAKMLR
DWARRHAIKDFFDVGHNGVCHALFPEKGYIRPGYTVIMGDSHTCTHGAFGAFAAGVGTTDLEVGILKGVCAFREPKSIRI
NLNGTLPKGVYAKDVILYVIGQLGVNGATDRVMEFRGPVVDAMTMESRMTLCNMAIEAGGTSGICMPDMVTVEYLWPFIE
NEYPSKEAALAEFSTWRSDDDAVYERVLDFDVSVLEPIVTFGYKPDQVKPISEIAGSPVDQVYLGSCTNGRLEDLRIAAR
ILKGKKIAPSVRGILSPATPKIYKDAMAEGLIDIFMEAGFCVTNPTCGACLGMSNGVLAEGEVCASTTNRNFMGRMGKGG
MVHLMSPATSAATAIEGVIADPRKYL

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=454, Percent_Identity=28.1938325991189, Blast_Score=115, Evalue=6e-26,
Organism=Escherichia coli, GI1786259, Length=468, Percent_Identity=37.6068376068376, Blast_Score=291, Evalue=6e-80,
Organism=Escherichia coli, GI2367097, Length=380, Percent_Identity=25, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI87081781, Length=457, Percent_Identity=22.9759299781182, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787531, Length=337, Percent_Identity=25.2225519287834, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25149337, Length=454, Percent_Identity=29.295154185022, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32564738, Length=338, Percent_Identity=30.7692307692308, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI25149342, Length=279, Percent_Identity=30.8243727598566, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6321429, Length=476, Percent_Identity=35.2941176470588, Blast_Score=271, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6320440, Length=456, Percent_Identity=31.359649122807, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6322261, Length=456, Percent_Identity=29.1666666666667, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6323335, Length=451, Percent_Identity=29.0465631929047, Blast_Score=137, Evalue=2e-33,
Organism=Drosophila melanogaster, GI28571643, Length=457, Percent_Identity=27.5711159737418, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI281365315, Length=448, Percent_Identity=27.6785714285714, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI17864292, Length=448, Percent_Identity=27.6785714285714, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI161076999, Length=338, Percent_Identity=28.9940828402367, Blast_Score=102, Evalue=4e-22,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_GEOMG (Q39W70)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_384229.1
- STRING:   Q39W70
- GeneID:   3739767
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1268
- NMPDR:   fig|269799.3.peg.1946
- eggNOG:   COG0065
- HOGENOM:   HBG330745
- OMA:   RQGIEHV
- PhylomeDB:   Q39W70
- ProtClustDB:   PRK00402
- BioCyc:   GMET269799:GMET_1268-MONOMER
- HAMAP:   MF_01027
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR01343
- TIGRFAMs:   TIGR02086

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 46165; Mature: 46034

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTTAEKIFASHLVDEPFSGTKVLRLDVVMCHEITTPIAIADLMARGKDRVFDPTKIKA
CCCHHHHHHHHHHHHCCCCCCCEEEEEEEEHHHHHCCHHHHHHHHHCCCCCCCCHHHHHH
VIDHVTPSKDSKTATQAKMLRDWARRHAIKDFFDVGHNGVCHALFPEKGYIRPGYTVIMG
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEC
DSHTCTHGAFGAFAAGVGTTDLEVGILKGVCAFREPKSIRINLNGTLPKGVYAKDVILYV
CCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHH
IGQLGVNGATDRVMEFRGPVVDAMTMESRMTLCNMAIEAGGTSGICMPDMVTVEYLWPFI
HHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
ENEYPSKEAALAEFSTWRSDDDAVYERVLDFDVSVLEPIVTFGYKPDQVKPISEIAGSPV
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHCCCCC
DQVYLGSCTNGRLEDLRIAARILKGKKIAPSVRGILSPATPKIYKDAMAEGLIDIFMEAG
CEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
FCVTNPTCGACLGMSNGVLAEGEVCASTTNRNFMGRMGKGGMVHLMSPATSAATAIEGVI
CEECCCCCHHHHCCCCCCEECCCEEECCCCCCHHCCCCCCCEEEEECCCHHHHHHHHHHH
ADPRKYL
CCHHHCC
>Mature Secondary Structure 
GKTTAEKIFASHLVDEPFSGTKVLRLDVVMCHEITTPIAIADLMARGKDRVFDPTKIKA
CCHHHHHHHHHHHHCCCCCCCEEEEEEEEHHHHHCCHHHHHHHHHCCCCCCCCHHHHHH
VIDHVTPSKDSKTATQAKMLRDWARRHAIKDFFDVGHNGVCHALFPEKGYIRPGYTVIMG
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEC
DSHTCTHGAFGAFAAGVGTTDLEVGILKGVCAFREPKSIRINLNGTLPKGVYAKDVILYV
CCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHH
IGQLGVNGATDRVMEFRGPVVDAMTMESRMTLCNMAIEAGGTSGICMPDMVTVEYLWPFI
HHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
ENEYPSKEAALAEFSTWRSDDDAVYERVLDFDVSVLEPIVTFGYKPDQVKPISEIAGSPV
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCHHHHCCCCC
DQVYLGSCTNGRLEDLRIAARILKGKKIAPSVRGILSPATPKIYKDAMAEGLIDIFMEAG
CEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
FCVTNPTCGACLGMSNGVLAEGEVCASTTNRNFMGRMGKGGMVHLMSPATSAATAIEGVI
CEECCCCCHHHHCCCCCCEECCCEEECCCCCCHHCCCCCCCEEEEECCCHHHHHHHHHHH
ADPRKYL
CCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA