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The map label for this gene is leuD [H]

Identifier: 78222483

GI number: 78222483

Start: 1430155

End: 1430682

Strand: Direct

Name: leuD [H]

Synonym: Gmet_1269

Alternate gene names: 78222483

Gene position: 1430155-1430682 (Clockwise)

Preceding gene: 78222482

Following gene: 78222484

Centisome position: 35.78

GC content: 54.36

Gene sequence:

>528_bases
ATGAAACAATTTGGCGGCCCCGTACTCTTTCTCGACCGCGCCGATATCAATACCGATGAGATCATCCCGGCCAAGTACCT
GACCGAAATCACGAAGGAAGATCTGAAACCCTATATTCTTGAGGACTTGAAGCTCCCGGGCTTCGATCCGAAGGGTGAGA
AGACCAGGTCCGCACGGGTCATCGTGTCCCGGGCAAACTTTGGGTGCGGTTCCTCCCGGGAGCATGCACCCTGGGTTTTC
GAGGTCAACGGCATCACCGCCGTTATCGCGGAGTCTTTTGCCCGGATTTTCCGACAGAACATGTTCAACTGTGGCATGGC
AGCCATCGAGTTGCCGAAGGAGAAGATCGATCTCCTCTTTTCCTTTGACAACTGCCCTGACACCGCCATTGGCATCGATG
TTGAGAAGCAGACCCTGACCATTTCGGGAGGAGGAAGGAAAGAGATAATTACTTTCGATGTTTCACCTTTCGACAGGGCC
CTCGTCCTCGCGGGCGGGTGGGTCGACTATGCCGACCAGAAATACTGA

Upstream 100 bases:

>100_bases
CGCCGATCCCCGGAAGTACCTGTAATCATGCAAATGGATGGGACGGTAGCCCTTTTGGATACTGGCCACCTGTGACCGAC
TTTTTCAAGGAGAATGCACC

Downstream 100 bases:

>100_bases
GCAGTAGATCTACCCCGTGAGCATTTCCGAGAGGCCTGCGTCATACGTGGGCCTCTTTCTCTATGAACGGGCGCTAGAGG
GGATTTTTTGTTGACTCACC

Product: 3-isopropylmalate dehydratase, small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MKQFGGPVLFLDRADINTDEIIPAKYLTEITKEDLKPYILEDLKLPGFDPKGEKTRSARVIVSRANFGCGSSREHAPWVF
EVNGITAVIAESFARIFRQNMFNCGMAAIELPKEKIDLLFSFDNCPDTAIGIDVEKQTLTISGGGRKEIITFDVSPFDRA
LVLAGGWVDYADQKY

Sequences:

>Translated_175_residues
MKQFGGPVLFLDRADINTDEIIPAKYLTEITKEDLKPYILEDLKLPGFDPKGEKTRSARVIVSRANFGCGSSREHAPWVF
EVNGITAVIAESFARIFRQNMFNCGMAAIELPKEKIDLLFSFDNCPDTAIGIDVEKQTLTISGGGRKEIITFDVSPFDRA
LVLAGGWVDYADQKY
>Mature_175_residues
MKQFGGPVLFLDRADINTDEIIPAKYLTEITKEDLKPYILEDLKLPGFDPKGEKTRSARVIVSRANFGCGSSREHAPWVF
EVNGITAVIAESFARIFRQNMFNCGMAAIELPKEKIDLLFSFDNCPDTAIGIDVEKQTLTISGGGRKEIITFDVSPFDRA
LVLAGGWVDYADQKY

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=171, Percent_Identity=32.1637426900585, Blast_Score=94, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6321429, Length=167, Percent_Identity=32.3353293413174, Blast_Score=78, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6320440, Length=114, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 19499; Mature: 19499

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQFGGPVLFLDRADINTDEIIPAKYLTEITKEDLKPYILEDLKLPGFDPKGEKTRSARV
CCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCEEE
IVSRANFGCGSSREHAPWVFEVNGITAVIAESFARIFRQNMFNCGMAAIELPKEKIDLLF
EEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCHHEEECCHHHEEEEE
SFDNCPDTAIGIDVEKQTLTISGGGRKEIITFDVSPFDRALVLAGGWVDYADQKY
EECCCCCCEEEEEEECEEEEEECCCCEEEEEEECCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MKQFGGPVLFLDRADINTDEIIPAKYLTEITKEDLKPYILEDLKLPGFDPKGEKTRSARV
CCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCEEE
IVSRANFGCGSSREHAPWVFEVNGITAVIAESFARIFRQNMFNCGMAAIELPKEKIDLLF
EEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCHHEEECCHHHEEEEE
SFDNCPDTAIGIDVEKQTLTISGGGRKEIITFDVSPFDRALVLAGGWVDYADQKY
EECCCCCCEEEEEEECEEEEEECCCCEEEEEEECCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA