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The map label for this gene is leuA [H]

Identifier: 78222479

GI number: 78222479

Start: 1425537

End: 1427075

Strand: Direct

Name: leuA [H]

Synonym: Gmet_1265

Alternate gene names: 78222479

Gene position: 1425537-1427075 (Clockwise)

Preceding gene: 78222478

Following gene: 78222482

Centisome position: 35.66

GC content: 54.78

Gene sequence:

>1539_bases
ATGGCGGAACTGAAGACCATCAAGATTTTCGATACAACGCTTCGCGACGGTGAGCAGTCGCCGGGCAACAGCATGAACAT
CGATGAGAAACTGCGCGTTGCCAAGCAGCTTCTGAAGCTGAACGTCGATGTCATCGAAGCGGGATTTCCCATCGCTTCCG
AGGGGGATTTCGAGGCGGTCAAACGGGTTGCCCAGACCATCAAGGGACCGGAGATCGCTGGCCTTTGCCGTTCCAGCGAC
AAGGATATCGACCGGGCCTGGGAGGCACTTAAGTACGCAGGTGAGAAGGGGCGTATCCATACTTTTATCGCCACCTCTGA
TATCCACATGAAGTACAAGCTGAAGATGACTCGTGAGCAGGTGCTTGAGAATGCGGTCAAAGCGGTGAAGCGAGCGAAAT
CCTACACGCCAAATGTGGAATTTTCCTGCGAGGATGCCGTCCGTACCGACCTGGCCTTCTTGGTGCAAATGGTGGAGGCG
GTAATCGACGCCGGAGTCACGACGGTGAATATTCCCGATACTGTCGGCTACACGATCCCCTTCGAGTACTTCAACATCAT
CCGTCACCTCAAGGAAAACGTTTCCAATATTGAAAAGGCGGTCATTTCGGTCCATTGCCACAATGACCTGGGGCTTTCCG
TGGCGAACTCCATTGCAGCGGTTCAGGCTGGGGCAGGTCAGGTGGAGTGTACCATCAACGGTATTGGTGAGCGGGCGGGA
AACTGCTCCCTTGAAGAGTTCGTCATGGCTATCAAGACCCGCCACGACATCCTTCCCTTCAGGACGAACGTTGACCACGA
ACAGATCGTCCCTGCCAGTCGGCTTCTCTCCACTATTACCGGCATCGGAGTTCAGCCCAACAAGGCGATTGTAGGGGCCA
ACGCCTTTGCCCATGAGTCGGGCATACATCAGCATGGCGTGCTCATGGAGAAATCGACCTACGAAATCATGACTCCGGAA
TCAATCGGGCTCAAGACGAACGTTTTGGTGCTCGGCAAGCATTCGGGACGTCACGCATTCAAGAAGCGGCTTGCGGAACT
TGGGTACGAGCTTTCCGATGAAGACCTGAACCGCGCCTTTGAGCGTTTCAAGGCCTTGGCTGATCTGAAGAAAGAGGTTT
TCGACGAGGATCTTGACGCATTGGTTGCAGACGAAGTGATGCGTCCGGCGGAGAAGTTCCGGCTCTCTCATATCACGGTC
ACGTGCGGTTCCTTTGCGGTCGCCACTTCGACGGTGCAAATGGAGATTGACGGTACGGCGGTGCGAACTGCCGAACTGGG
GGATGGCCCGGTTGATGCGACTCTCAAGGCAATCAAGAAGCTGACCAAGACCAAGGCGAAGCTTCAGCAGTATAATGTCG
GATCCATTACCGGCGGGACCGATGCCCAAGGCGAAGTGACCGTGCGGGTGACAGACGGCAACGAAACGGTTGTGGGGCGA
GGTTCTTCCACAGACATAATTGAGGCGTCGGCAAAGGCTTATATCAACGCGCTCAATCGACTCAATGCAAAGATAGAGCG
AATCCACGAAAGTCTGTGA

Upstream 100 bases:

>100_bases
GGGCGAAGGCTTTTTTTATTTTTGCGCTCACGTGAACACTGACGGCCTTGATCAACCCGTCGGTGGCATGAGACATCCAT
CACGCAAAGGGAGGTAGACC

Downstream 100 bases:

>100_bases
TCTGATGTCGGTGAGAGACCTCGGTGGCTCTCGCCGACAGTTGAGATTTCAATGAGGCGCTTGTCTGAAAAAACGAAACC
CCGCTGAATAATTCAGCGGG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MAELKTIKIFDTTLRDGEQSPGNSMNIDEKLRVAKQLLKLNVDVIEAGFPIASEGDFEAVKRVAQTIKGPEIAGLCRSSD
KDIDRAWEALKYAGEKGRIHTFIATSDIHMKYKLKMTREQVLENAVKAVKRAKSYTPNVEFSCEDAVRTDLAFLVQMVEA
VIDAGVTTVNIPDTVGYTIPFEYFNIIRHLKENVSNIEKAVISVHCHNDLGLSVANSIAAVQAGAGQVECTINGIGERAG
NCSLEEFVMAIKTRHDILPFRTNVDHEQIVPASRLLSTITGIGVQPNKAIVGANAFAHESGIHQHGVLMEKSTYEIMTPE
SIGLKTNVLVLGKHSGRHAFKKRLAELGYELSDEDLNRAFERFKALADLKKEVFDEDLDALVADEVMRPAEKFRLSHITV
TCGSFAVATSTVQMEIDGTAVRTAELGDGPVDATLKAIKKLTKTKAKLQQYNVGSITGGTDAQGEVTVRVTDGNETVVGR
GSSTDIIEASAKAYINALNRLNAKIERIHESL

Sequences:

>Translated_512_residues
MAELKTIKIFDTTLRDGEQSPGNSMNIDEKLRVAKQLLKLNVDVIEAGFPIASEGDFEAVKRVAQTIKGPEIAGLCRSSD
KDIDRAWEALKYAGEKGRIHTFIATSDIHMKYKLKMTREQVLENAVKAVKRAKSYTPNVEFSCEDAVRTDLAFLVQMVEA
VIDAGVTTVNIPDTVGYTIPFEYFNIIRHLKENVSNIEKAVISVHCHNDLGLSVANSIAAVQAGAGQVECTINGIGERAG
NCSLEEFVMAIKTRHDILPFRTNVDHEQIVPASRLLSTITGIGVQPNKAIVGANAFAHESGIHQHGVLMEKSTYEIMTPE
SIGLKTNVLVLGKHSGRHAFKKRLAELGYELSDEDLNRAFERFKALADLKKEVFDEDLDALVADEVMRPAEKFRLSHITV
TCGSFAVATSTVQMEIDGTAVRTAELGDGPVDATLKAIKKLTKTKAKLQQYNVGSITGGTDAQGEVTVRVTDGNETVVGR
GSSTDIIEASAKAYINALNRLNAKIERIHESL
>Mature_511_residues
AELKTIKIFDTTLRDGEQSPGNSMNIDEKLRVAKQLLKLNVDVIEAGFPIASEGDFEAVKRVAQTIKGPEIAGLCRSSDK
DIDRAWEALKYAGEKGRIHTFIATSDIHMKYKLKMTREQVLENAVKAVKRAKSYTPNVEFSCEDAVRTDLAFLVQMVEAV
IDAGVTTVNIPDTVGYTIPFEYFNIIRHLKENVSNIEKAVISVHCHNDLGLSVANSIAAVQAGAGQVECTINGIGERAGN
CSLEEFVMAIKTRHDILPFRTNVDHEQIVPASRLLSTITGIGVQPNKAIVGANAFAHESGIHQHGVLMEKSTYEIMTPES
IGLKTNVLVLGKHSGRHAFKKRLAELGYELSDEDLNRAFERFKALADLKKEVFDEDLDALVADEVMRPAEKFRLSHITVT
CGSFAVATSTVQMEIDGTAVRTAELGDGPVDATLKAIKKLTKTKAKLQQYNVGSITGGTDAQGEVTVRVTDGNETVVGRG
SSTDIIEASAKAYINALNRLNAKIERIHESL

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=499, Percent_Identity=50.3006012024048, Blast_Score=476, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6320071, Length=388, Percent_Identity=30.1546391752577, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6320019, Length=396, Percent_Identity=29.5454545454545, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6324682, Length=570, Percent_Identity=25.4385964912281, Blast_Score=140, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6324225, Length=543, Percent_Identity=26.3351749539595, Blast_Score=132, Evalue=1e-31,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 55904; Mature: 55773

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELKTIKIFDTTLRDGEQSPGNSMNIDEKLRVAKQLLKLNVDVIEAGFPIASEGDFEAV
CCCCEEEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHEECCCCCCCCCCHHHH
KRVAQTIKGPEIAGLCRSSDKDIDRAWEALKYAGEKGRIHTFIATSDIHMKYKLKMTREQ
HHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCEEEEEEEHHHHHH
VLENAVKAVKRAKSYTPNVEFSCEDAVRTDLAFLVQMVEAVIDAGVTTVNIPDTVGYTIP
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEC
FEYFNIIRHLKENVSNIEKAVISVHCHNDLGLSVANSIAAVQAGAGQVECTINGIGERAG
HHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCC
NCSLEEFVMAIKTRHDILPFRTNVDHEQIVPASRLLSTITGIGVQPNKAIVGANAFAHES
CCCHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHHHCCCCCCCCEEEECCHHHHHC
GIHQHGVLMEKSTYEIMTPESIGLKTNVLVLGKHSGRHAFKKRLAELGYELSDEDLNRAF
CCCCCCEEEECCCEEEECCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHH
ERFKALADLKKEVFDEDLDALVADEVMRPAEKFRLSHITVTCGSFAVATSTVQMEIDGTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHEEEEEEEEECCEEEEEEEEEEEECCCE
VRTAELGDGPVDATLKAIKKLTKTKAKLQQYNVGSITGGTDAQGEVTVRVTDGNETVVGR
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCEEEEEC
GSSTDIIEASAKAYINALNRLNAKIERIHESL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AELKTIKIFDTTLRDGEQSPGNSMNIDEKLRVAKQLLKLNVDVIEAGFPIASEGDFEAV
CCCEEEEEEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHEECCCCCCCCCCHHHH
KRVAQTIKGPEIAGLCRSSDKDIDRAWEALKYAGEKGRIHTFIATSDIHMKYKLKMTREQ
HHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCEEEEEEEHHHHHH
VLENAVKAVKRAKSYTPNVEFSCEDAVRTDLAFLVQMVEAVIDAGVTTVNIPDTVGYTIP
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEC
FEYFNIIRHLKENVSNIEKAVISVHCHNDLGLSVANSIAAVQAGAGQVECTINGIGERAG
HHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCC
NCSLEEFVMAIKTRHDILPFRTNVDHEQIVPASRLLSTITGIGVQPNKAIVGANAFAHES
CCCHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHHHCCCCCCCCEEEECCHHHHHC
GIHQHGVLMEKSTYEIMTPESIGLKTNVLVLGKHSGRHAFKKRLAELGYELSDEDLNRAF
CCCCCCEEEECCCEEEECCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHH
ERFKALADLKKEVFDEDLDALVADEVMRPAEKFRLSHITVTCGSFAVATSTVQMEIDGTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHEEEEEEEEECCEEEEEEEEEEEECCCE
VRTAELGDGPVDATLKAIKKLTKTKAKLQQYNVGSITGGTDAQGEVTVRVTDGNETVVGR
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCEEEEEC
GSSTDIIEASAKAYINALNRLNAKIERIHESL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA