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The map label for this gene is appB [H]

Identifier: 78222449

GI number: 78222449

Start: 1393783

End: 1394784

Strand: Direct

Name: appB [H]

Synonym: Gmet_1235

Alternate gene names: 78222449

Gene position: 1393783-1394784 (Clockwise)

Preceding gene: 78222448

Following gene: 78222450

Centisome position: 34.87

GC content: 61.68

Gene sequence:

>1002_bases
ATGGTCCCCGTTCTCCGACTTATGATCACTTATCTTGCAAAGCGACTAATAATGATGGTTCCGCTCCTCCTGGGGATTAC
CCTCATCTCCTTTGTGGTGATCCGCCTGGCCCCCGGCGAGCCGGTGGAGGCCATGACCGCCATGAGCCCCAAAATCACCG
CCGAGACCCGCCAGCGGCTCCGGGAGTTCTATGGTCTCGACAAGCCGCTCCACGTCCAGTACGCCACCTGGCTCAAACAG
GTGGCCACCCTCGATTTCGGCCGCTCTTTCGCCCCCGACAATCGGCCGGTGACCGACAAGATCAAGGAACGGATTCCGGT
TACCATTTCCCTCAACGTCATCGCCCTGATCCTGGAGTTCGGCCTTGCCATACCCATCGGCATCTTTGCGGCCACCCACC
GGGACACCCTCTACGACAAGGGGATCACCATCTTCGTCTTCCTGGGGTTCGCGGTGCCGACCTTCTGGCTGGCGCTGCTC
CTCATGTACTTCTTCGGCGTTAAGTTGGGGTGGCTCCCCATCTCGGGGCTCCACTCTCTGGGGAGCGAGACACTTTCCCC
TATGGCGTGGTTCGTGGATTTGGCGAAGCACCTGATCCTCCCGGTCTCCGTGGCCACCTTCGGGAGTCTGGCGGGGCTCT
CCCGCTACATGCGCTCCACCATGCTGGAAGTGATCCGACAGGATTACATCACCACGGCCCGGGCCAAGGGTCTCACGGAG
CGGGCGGTCATCTACCGGCACGCCCTGCGCAATGCGCTGCTGCCGGTCATCACGCTCCTCGGCTTCTCCCTTCCCGGCCT
CATCGGGGGGAGCGTCATTTTCGAGACCATCTTCGCCATCCCCGGCATGGGGCAGCTCTTCTACCAGGGGGTCATGGGGC
GGGATTATCCCCTTGTCATGGGAATTCTGGTGATTGGCGCGTTCCTTACCCTTGTCGGCAATCTCCTGGCCGATCTCTGC
TATGCCATGGCCGATCCGCGTATCAGGCACGGGAGGGGGTAG

Upstream 100 bases:

>100_bases
TCATCCGGTGGTATGTGCCCAAGGAAGAGCAGGTGCATTAACTGCCGGGACCAGGTGCCGGGGACCAGTAAAGGCTTAAA
TCTTTACCGGCCCACGGTTC

Downstream 100 bases:

>100_bases
GAAATGGCCTTTCGGGAAAGCTGGTTCCATACGGTTTTCTGGAAGCGGTTCCGGCACAACCGGTTTGCCATGGCCGGCGG
GTGTGTCGTGCTCCTTCTCT

Product: binding-protein dependent transport system inner membrane protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MVPVLRLMITYLAKRLIMMVPLLLGITLISFVVIRLAPGEPVEAMTAMSPKITAETRQRLREFYGLDKPLHVQYATWLKQ
VATLDFGRSFAPDNRPVTDKIKERIPVTISLNVIALILEFGLAIPIGIFAATHRDTLYDKGITIFVFLGFAVPTFWLALL
LMYFFGVKLGWLPISGLHSLGSETLSPMAWFVDLAKHLILPVSVATFGSLAGLSRYMRSTMLEVIRQDYITTARAKGLTE
RAVIYRHALRNALLPVITLLGFSLPGLIGGSVIFETIFAIPGMGQLFYQGVMGRDYPLVMGILVIGAFLTLVGNLLADLC
YAMADPRIRHGRG

Sequences:

>Translated_333_residues
MVPVLRLMITYLAKRLIMMVPLLLGITLISFVVIRLAPGEPVEAMTAMSPKITAETRQRLREFYGLDKPLHVQYATWLKQ
VATLDFGRSFAPDNRPVTDKIKERIPVTISLNVIALILEFGLAIPIGIFAATHRDTLYDKGITIFVFLGFAVPTFWLALL
LMYFFGVKLGWLPISGLHSLGSETLSPMAWFVDLAKHLILPVSVATFGSLAGLSRYMRSTMLEVIRQDYITTARAKGLTE
RAVIYRHALRNALLPVITLLGFSLPGLIGGSVIFETIFAIPGMGQLFYQGVMGRDYPLVMGILVIGAFLTLVGNLLADLC
YAMADPRIRHGRG
>Mature_333_residues
MVPVLRLMITYLAKRLIMMVPLLLGITLISFVVIRLAPGEPVEAMTAMSPKITAETRQRLREFYGLDKPLHVQYATWLKQ
VATLDFGRSFAPDNRPVTDKIKERIPVTISLNVIALILEFGLAIPIGIFAATHRDTLYDKGITIFVFLGFAVPTFWLALL
LMYFFGVKLGWLPISGLHSLGSETLSPMAWFVDLAKHLILPVSVATFGSLAGLSRYMRSTMLEVIRQDYITTARAKGLTE
RAVIYRHALRNALLPVITLLGFSLPGLIGGSVIFETIFAIPGMGQLFYQGVMGRDYPLVMGILVIGAFLTLVGNLLADLC
YAMADPRIRHGRG

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789888, Length=331, Percent_Identity=39.5770392749245, Blast_Score=224, Evalue=5e-60,
Organism=Escherichia coli, GI1787497, Length=323, Percent_Identity=35.2941176470588, Blast_Score=207, Evalue=8e-55,
Organism=Escherichia coli, GI1789965, Length=341, Percent_Identity=36.0703812316716, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1787053, Length=323, Percent_Identity=31.8885448916409, Blast_Score=188, Evalue=5e-49,
Organism=Escherichia coli, GI1788504, Length=371, Percent_Identity=30.4582210242588, Blast_Score=178, Evalue=4e-46,
Organism=Escherichia coli, GI1787761, Length=340, Percent_Identity=34.4117647058824, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1787550, Length=275, Percent_Identity=32.3636363636364, Blast_Score=146, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 36869; Mature: 36869

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPVLRLMITYLAKRLIMMVPLLLGITLISFVVIRLAPGEPVEAMTAMSPKITAETRQRL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHH
REFYGLDKPLHVQYATWLKQVATLDFGRSFAPDNRPVTDKIKERIPVTISLNVIALILEF
HHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCEEEHHHHHHHHHHH
GLAIPIGIFAATHRDTLYDKGITIFVFLGFAVPTFWLALLLMYFFGVKLGWLPISGLHSL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
GSETLSPMAWFVDLAKHLILPVSVATFGSLAGLSRYMRSTMLEVIRQDYITTARAKGLTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
RAVIYRHALRNALLPVITLLGFSLPGLIGGSVIFETIFAIPGMGQLFYQGVMGRDYPLVM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHH
GILVIGAFLTLVGNLLADLCYAMADPRIRHGRG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MVPVLRLMITYLAKRLIMMVPLLLGITLISFVVIRLAPGEPVEAMTAMSPKITAETRQRL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHH
REFYGLDKPLHVQYATWLKQVATLDFGRSFAPDNRPVTDKIKERIPVTISLNVIALILEF
HHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCEEEHHHHHHHHHHH
GLAIPIGIFAATHRDTLYDKGITIFVFLGFAVPTFWLALLLMYFFGVKLGWLPISGLHSL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
GSETLSPMAWFVDLAKHLILPVSVATFGSLAGLSRYMRSTMLEVIRQDYITTARAKGLTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
RAVIYRHALRNALLPVITLLGFSLPGLIGGSVIFETIFAIPGMGQLFYQGVMGRDYPLVM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHH
GILVIGAFLTLVGNLLADLCYAMADPRIRHGRG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]