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The map label for this gene is appA [H]

Identifier: 78222448

GI number: 78222448

Start: 1392107

End: 1393723

Strand: Direct

Name: appA [H]

Synonym: Gmet_1234

Alternate gene names: 78222448

Gene position: 1392107-1393723 (Clockwise)

Preceding gene: 78222447

Following gene: 78222449

Centisome position: 34.83

GC content: 61.6

Gene sequence:

>1617_bases
ATGACGGCACGGCTGTTCTGCTGTCTTGCGGCACTGGTTACCCTTGTTTCGTGCAGCGGCGGTACGCCTCCTGCGGAACT
GCCGCGGCGTGGAGCAGGGGCCCCTGCCTACGGCGATGCCCTGGTGGTTGGCACCATTGGCGAACCATCCAACCTGATCC
CGCTGCTTGCCTCCGATTCCGCCTCCCACGATGTGGCGGGGAACGTCTTCAACGGCCTTGTGAAGTATGACAAGAATCTT
GAGCTGGTGGGGGATCTGGCGGAGTCGTGGCAGGTATCCCCCGACGGCCTCACCATCACCTTCAGATTGCGCAAAGGGGT
GAAGTGGCACGACGGCACCGAGTTCACCTCCCGCGACGCCCTCTACACCTACCGCGTCACCATCGATCCCAAGACCCCCA
CTGCCTATGCCGAGGACTTCAAGCAGGTGAAGAGCGCAGAAGCGCCGGACCGGTACACCTTCCGCGTGACTTACGGCAAG
CCCTTTGCCCCGGCTCTGGCCTCCTGGGGGGCGGGAATCCTGCCGGCCCACCTCCTTGAGGGGAAGGACATCACGAAAAG
CCCACTTTCCCGCCATCCCGTGGGGACCGGCCCCTACCGGTTCAAGGAGTGGATTGCGGGGCAGAAGGTCGTCCTTGAGG
CATTTCCCGACTACTTCGAGGGGCGGCCCTACATTGACCGGTTCGTCTATCGGATTATCCCCGATACTTCCACCATGTAC
CTGGAACTGAAGGCGGGCGGCCTCGACATGATGGGGCTTACGCCGGTCCAGTACGCCCGGCAGACCGATACCCCCGATTT
CCTGGCCCGTTTCAATAAGTTCCGCTATCCGGCCTCTGCGTACACCTACCTGGGATACAATCTCCGCAACCCTCTTTTCG
CCGACCGGCGGGTCCGACAGGCCATTGCCTGCGCCATCAACAAGGACGAGATAGTCCACGGGGTGCTTCTGGGGCTGGGG
CAGGTGGCCCACGGGCCCTTCAAGCCGGGAACCTGGCCATACAACCCTTCCGTGAGGGACTTCGGCTACGACCCGGCCCG
GGCAAAGGCGTTGCTGGCAGAGGCGGGGTGGCACGCGGTCGGGCCCGATGGTATCCTTACGAAAGACGGCAAGCCCTTCA
GTTTCACGATTTTTACCAACCAGGGAAACGACCAGCGGCTCAAGACCGCCCAGATCATCCAGCGTCGCCTCGCAAAGGTT
GGGATCGAGGTGAAGATCCGGGTTCTGGAGTGGGCGTCGCTTCTCACCAACTTCATCGACAAGCGGAATTTCGACGCCCT
CATCATGGGATGGACCATTCCCCAGGATCCGGATATCTTTGATGTGTGGCATTCCAGCAAGACCGGCCCCAAGGAACTGA
ACTTCATCGGGTTCAAGAACGCCGAGGTGGACCGGCTCATCGAAGAGGGGCGCAGTACCTTCGATCAGGAAAAGCGCAGG
CGCTGCTACTGGCGCATCCAGGAAATACTCGCCCAGGAGCAGCCCTACACGTTTCTCTTCGTCCCCGATGCCCTGCCGGT
CGTGAATGCCCGCTTCCGGGGGATCGAGCCAGCGCCGGCCGGAATCATGCACAACATCATCCGGTGGTATGTGCCCAAGG
AAGAGCAGGTGCATTAA

Upstream 100 bases:

>100_bases
GCAATGCCTTGAAACTTTACAAAAAGATCCTCGACGCCGACCCGGGTCGCAAGGATATCGCCGAAAAGAAGAAAAAGGTC
AGGGCGGAAAGTCTGGAAAA

Downstream 100 bases:

>100_bases
CTGCCGGGACCAGGTGCCGGGGACCAGTAAAGGCTTAAATCTTTACCGGCCCACGGTTCATGGTCCCCGTTCTCCGACTT
ATGATCACTTATCTTGCAAA

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MTARLFCCLAALVTLVSCSGGTPPAELPRRGAGAPAYGDALVVGTIGEPSNLIPLLASDSASHDVAGNVFNGLVKYDKNL
ELVGDLAESWQVSPDGLTITFRLRKGVKWHDGTEFTSRDALYTYRVTIDPKTPTAYAEDFKQVKSAEAPDRYTFRVTYGK
PFAPALASWGAGILPAHLLEGKDITKSPLSRHPVGTGPYRFKEWIAGQKVVLEAFPDYFEGRPYIDRFVYRIIPDTSTMY
LELKAGGLDMMGLTPVQYARQTDTPDFLARFNKFRYPASAYTYLGYNLRNPLFADRRVRQAIACAINKDEIVHGVLLGLG
QVAHGPFKPGTWPYNPSVRDFGYDPARAKALLAEAGWHAVGPDGILTKDGKPFSFTIFTNQGNDQRLKTAQIIQRRLAKV
GIEVKIRVLEWASLLTNFIDKRNFDALIMGWTIPQDPDIFDVWHSSKTGPKELNFIGFKNAEVDRLIEEGRSTFDQEKRR
RCYWRIQEILAQEQPYTFLFVPDALPVVNARFRGIEPAPAGIMHNIIRWYVPKEEQVH

Sequences:

>Translated_538_residues
MTARLFCCLAALVTLVSCSGGTPPAELPRRGAGAPAYGDALVVGTIGEPSNLIPLLASDSASHDVAGNVFNGLVKYDKNL
ELVGDLAESWQVSPDGLTITFRLRKGVKWHDGTEFTSRDALYTYRVTIDPKTPTAYAEDFKQVKSAEAPDRYTFRVTYGK
PFAPALASWGAGILPAHLLEGKDITKSPLSRHPVGTGPYRFKEWIAGQKVVLEAFPDYFEGRPYIDRFVYRIIPDTSTMY
LELKAGGLDMMGLTPVQYARQTDTPDFLARFNKFRYPASAYTYLGYNLRNPLFADRRVRQAIACAINKDEIVHGVLLGLG
QVAHGPFKPGTWPYNPSVRDFGYDPARAKALLAEAGWHAVGPDGILTKDGKPFSFTIFTNQGNDQRLKTAQIIQRRLAKV
GIEVKIRVLEWASLLTNFIDKRNFDALIMGWTIPQDPDIFDVWHSSKTGPKELNFIGFKNAEVDRLIEEGRSTFDQEKRR
RCYWRIQEILAQEQPYTFLFVPDALPVVNARFRGIEPAPAGIMHNIIRWYVPKEEQVH
>Mature_537_residues
TARLFCCLAALVTLVSCSGGTPPAELPRRGAGAPAYGDALVVGTIGEPSNLIPLLASDSASHDVAGNVFNGLVKYDKNLE
LVGDLAESWQVSPDGLTITFRLRKGVKWHDGTEFTSRDALYTYRVTIDPKTPTAYAEDFKQVKSAEAPDRYTFRVTYGKP
FAPALASWGAGILPAHLLEGKDITKSPLSRHPVGTGPYRFKEWIAGQKVVLEAFPDYFEGRPYIDRFVYRIIPDTSTMYL
ELKAGGLDMMGLTPVQYARQTDTPDFLARFNKFRYPASAYTYLGYNLRNPLFADRRVRQAIACAINKDEIVHGVLLGLGQ
VAHGPFKPGTWPYNPSVRDFGYDPARAKALLAEAGWHAVGPDGILTKDGKPFSFTIFTNQGNDQRLKTAQIIQRRLAKVG
IEVKIRVLEWASLLTNFIDKRNFDALIMGWTIPQDPDIFDVWHSSKTGPKELNFIGFKNAEVDRLIEEGRSTFDQEKRRR
CYWRIQEILAQEQPYTFLFVPDALPVVNARFRGIEPAPAGIMHNIIRWYVPKEEQVH

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=523, Percent_Identity=29.0630975143403, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1787762, Length=488, Percent_Identity=29.5081967213115, Blast_Score=181, Evalue=7e-47,
Organism=Escherichia coli, GI1787052, Length=478, Percent_Identity=28.2426778242678, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1789887, Length=443, Percent_Identity=27.313769751693, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1787551, Length=546, Percent_Identity=25.2747252747253, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI87081878, Length=533, Percent_Identity=25.328330206379, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1789397, Length=528, Percent_Identity=24.2424242424242, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1787495, Length=514, Percent_Identity=24.124513618677, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI87082063, Length=430, Percent_Identity=22.7906976744186, Blast_Score=89, Evalue=5e-19,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60296; Mature: 60164

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARLFCCLAALVTLVSCSGGTPPAELPRRGAGAPAYGDALVVGTIGEPSNLIPLLASDS
CCHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCC
ASHDVAGNVFNGLVKYDKNLELVGDLAESWQVSPDGLTITFRLRKGVKWHDGTEFTSRDA
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCC
LYTYRVTIDPKTPTAYAEDFKQVKSAEAPDRYTFRVTYGKPFAPALASWGAGILPAHLLE
EEEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHCCCCCCHHHHHC
GKDITKSPLSRHPVGTGPYRFKEWIAGQKVVLEAFPDYFEGRPYIDRFVYRIIPDTSTMY
CCCCCCCCHHCCCCCCCCHHHHHHHCCCHHHHHHCHHHHCCCHHHHHHHHHHCCCCCEEE
LELKAGGLDMMGLTPVQYARQTDTPDFLARFNKFRYPASAYTYLGYNLRNPLFADRRVRQ
EEEECCCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHH
AIACAINKDEIVHGVLLGLGQVAHGPFKPGTWPYNPSVRDFGYDPARAKALLAEAGWHAV
HHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHCCCCCC
GPDGILTKDGKPFSFTIFTNQGNDQRLKTAQIIQRRLAKVGIEVKIRVLEWASLLTNFID
CCCCEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHH
KRNFDALIMGWTIPQDPDIFDVWHSSKTGPKELNFIGFKNAEVDRLIEEGRSTFDQEKRR
CCCCCEEEEECCCCCCCCCEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHH
RCYWRIQEILAQEQPYTFLFVPDALPVVNARFRGIEPAPAGIMHNIIRWYVPKEEQVH
HHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TARLFCCLAALVTLVSCSGGTPPAELPRRGAGAPAYGDALVVGTIGEPSNLIPLLASDS
CHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCC
ASHDVAGNVFNGLVKYDKNLELVGDLAESWQVSPDGLTITFRLRKGVKWHDGTEFTSRDA
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCC
LYTYRVTIDPKTPTAYAEDFKQVKSAEAPDRYTFRVTYGKPFAPALASWGAGILPAHLLE
EEEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHCCCCCCHHHHHC
GKDITKSPLSRHPVGTGPYRFKEWIAGQKVVLEAFPDYFEGRPYIDRFVYRIIPDTSTMY
CCCCCCCCHHCCCCCCCCHHHHHHHCCCHHHHHHCHHHHCCCHHHHHHHHHHCCCCCEEE
LELKAGGLDMMGLTPVQYARQTDTPDFLARFNKFRYPASAYTYLGYNLRNPLFADRRVRQ
EEEECCCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHH
AIACAINKDEIVHGVLLGLGQVAHGPFKPGTWPYNPSVRDFGYDPARAKALLAEAGWHAV
HHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHCCCCCC
GPDGILTKDGKPFSFTIFTNQGNDQRLKTAQIIQRRLAKVGIEVKIRVLEWASLLTNFID
CCCCEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHH
KRNFDALIMGWTIPQDPDIFDVWHSSKTGPKELNFIGFKNAEVDRLIEEGRSTFDQEKRR
CCCCCEEEEECCCCCCCCCEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHH
RCYWRIQEILAQEQPYTFLFVPDALPVVNARFRGIEPAPAGIMHNIIRWYVPKEEQVH
HHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]