Click here to switch to the map view.

The map label for this gene is purU

Identifier: 78067580

GI number: 78067580

Start: 3255980

End: 3256864

Strand: Reverse

Name: purU

Synonym: Bcep18194_A6111

Alternate gene names: 78067580

Gene position: 3256864-3255980 (Counterclockwise)

Preceding gene: 78067581

Following gene: 78067577

Centisome position: 88.16

GC content: 64.75

Gene sequence:

>885_bases
ATGCCGACCGATCACAGCTTTATCTTGAAACTGTCGTGCCCCGACCGGCACGGCATCGTCCACGCGGTCTCGGGCTTCCT
GTTCGAGCGCAGCAACAACATCCTCGACTCGGCGCAGTTCGGCGACAGCCGCACCAGCGAATTCTTCATGCGCGTGCACT
TCGAGCAGGACGGCGGCGGCGCGGATGCCGCGACGGCGCTCGACACGCTGCGCCGGGAGTTCGCCCCGCTCGCCGAGCAG
TTCGCGATGCGCTGGGAAATGCACGACGCGGCCGTGAAGCCGCGCGTCGTGATCATGGTGTCGAAGATCGGCCACTGCCT
GAACGACCTGCTGTTCCGCTATCGCACCGGCCAGCTGCCGATCGAGATCCCGGCGATCGTGTCGAACCACAAGGAGTTCT
ACCAGCTCGCCGCGAGCTACAACATCCCGTTCCATCATTTCCCGCTGATCGGCGGTTCGTCCGATGCCGCGAAGGCCGCG
CAGGAAGCGCGCGTGCTGGAAGTGATCGACGAGCACCAGGCCGATCTGGTCGTGCTCGCGCGCTACATGCAGATCCTGTC
GCCGAACCTGTGCAAGCAGCTCGCCGGCCGCGCGATCAACATCCACCATTCGTTCCTGCCGAGCTTCAAGGGTGCGAAGC
CGTACTATCAGGCGTTCGATCGCGGCGTGAAGCTGATCGGTGCGACCGCGCACTACGTGACGACCGATCTCGACGAAGGC
CCGATCATCGAGCAGGAAGTCGAGCGCGTCGACCACAGCATGACGCCCGACCAGCTGACCGCAATCGGCCGCGACGTCGA
ATGCGTGACGCTTGCGCGCGCAGTGAAGTGGCACGTCGAGCACCGGGTCGTGCTGAACGGGACGAAGACGGTCGTGTTCC
GCTGA

Upstream 100 bases:

>100_bases
GACGCACTGTTCGCACCGGCCGCGTGACGCGTGCCGCGCATGCAGCCTGGCGGCGCGAACGGGCAATTTGACGAATACGG
AAACGAAGGGAGTAGCGGTC

Downstream 100 bases:

>100_bases
TTTGCTGATTGCCGGGCGTCGGCTGGCAATCACAAACAAAAACGCCGCGCCCTTCACGGGGCACGGCGTTTTTTATTGCT
TCTGACGCGCGGGCTGCTTC

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MPTDHSFILKLSCPDRHGIVHAVSGFLFERSNNILDSAQFGDSRTSEFFMRVHFEQDGGGADAATALDTLRREFAPLAEQ
FAMRWEMHDAAVKPRVVIMVSKIGHCLNDLLFRYRTGQLPIEIPAIVSNHKEFYQLAASYNIPFHHFPLIGGSSDAAKAA
QEARVLEVIDEHQADLVVLARYMQILSPNLCKQLAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEG
PIIEQEVERVDHSMTPDQLTAIGRDVECVTLARAVKWHVEHRVVLNGTKTVVFR

Sequences:

>Translated_294_residues
MPTDHSFILKLSCPDRHGIVHAVSGFLFERSNNILDSAQFGDSRTSEFFMRVHFEQDGGGADAATALDTLRREFAPLAEQ
FAMRWEMHDAAVKPRVVIMVSKIGHCLNDLLFRYRTGQLPIEIPAIVSNHKEFYQLAASYNIPFHHFPLIGGSSDAAKAA
QEARVLEVIDEHQADLVVLARYMQILSPNLCKQLAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEG
PIIEQEVERVDHSMTPDQLTAIGRDVECVTLARAVKWHVEHRVVLNGTKTVVFR
>Mature_293_residues
PTDHSFILKLSCPDRHGIVHAVSGFLFERSNNILDSAQFGDSRTSEFFMRVHFEQDGGGADAATALDTLRREFAPLAEQF
AMRWEMHDAAVKPRVVIMVSKIGHCLNDLLFRYRTGQLPIEIPAIVSNHKEFYQLAASYNIPFHHFPLIGGSSDAAKAAQ
EARVLEVIDEHQADLVVLARYMQILSPNLCKQLAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEGP
IIEQEVERVDHSMTPDQLTAIGRDVECVTLARAVKWHVEHRVVLNGTKTVVFR

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=168, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI209869995, Length=168, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI209869993, Length=168, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=9e-16,
Organism=Escherichia coli, GI1787483, Length=291, Percent_Identity=37.4570446735395, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1788846, Length=191, Percent_Identity=28.7958115183246, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17567511, Length=186, Percent_Identity=32.258064516129, Blast_Score=94, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32972; Mature: 32841

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTDHSFILKLSCPDRHGIVHAVSGFLFERSNNILDSAQFGDSRTSEFFMRVHFEQDGGG
CCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHCCCCCCCEEEEEEEEECCCCC
ADAATALDTLRREFAPLAEQFAMRWEMHDAAVKPRVVIMVSKIGHCLNDLLFRYRTGQLP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCCC
IEIPAIVSNHKEFYQLAASYNIPFHHFPLIGGSSDAAKAAQEARVLEVIDEHQADLVVLA
EEEHHHHHCHHHHHHHHHHCCCCCEECCEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
RYMQILSPNLCKQLAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEG
HHHHHHCHHHHHHHCCCEEEEEHHHCCCCCCCCHHHHHHHCCCEEEECCHHEEEECCCCC
PIIEQEVERVDHSMTPDQLTAIGRDVECVTLARAVKWHVEHRVVLNGTKTVVFR
CCCHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCEEEEECCEEEEEC
>Mature Secondary Structure 
PTDHSFILKLSCPDRHGIVHAVSGFLFERSNNILDSAQFGDSRTSEFFMRVHFEQDGGG
CCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHCCCCCCCEEEEEEEEECCCCC
ADAATALDTLRREFAPLAEQFAMRWEMHDAAVKPRVVIMVSKIGHCLNDLLFRYRTGQLP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCCC
IEIPAIVSNHKEFYQLAASYNIPFHHFPLIGGSSDAAKAAQEARVLEVIDEHQADLVVLA
EEEHHHHHCHHHHHHHHHHCCCCCEECCEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
RYMQILSPNLCKQLAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEG
HHHHHHCHHHHHHHCCCEEEEEHHHCCCCCCCCHHHHHHHCCCEEEECCHHEEEECCCCC
PIIEQEVERVDHSMTPDQLTAIGRDVECVTLARAVKWHVEHRVVLNGTKTVVFR
CCCHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCEEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]