BacMap

Definition	Burkholderia sp. 383 chromosome 1, complete genome.
Accession	NC_007510
Length	3,694,126

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The map label for this gene is paaF [H]

Identifier: 78067514

GI number: 78067514

Start: 3183764

End: 3184531

Strand: Reverse

Name: paaF [H]

Synonym: Bcep18194_A6045

Alternate gene names: 78067514

Gene position: 3184531-3183764 (Counterclockwise)

Preceding gene: 78067515

Following gene: 78067512

Centisome position: 86.21

GC content: 68.88

Gene sequence:

>768_bases
GTGGCCGAAATTCAAGTGGAACGCGCCGATGGCGTGATGACGATCACGATCGCGCGCCCGGCGAAGAAGAACGCGCTGAC
GGCGGCGATGTACCAGACGATGGCCGACGCACTCGCCGAAGCGCAGGAAGACAAGGCGGTTCGCGTGGTCCTGCTGCGCG
GTGGCGACGGCAACTTCACCGCGGGGAACGATCTCGAGGATTTCCTGAAGTCGCCGCCGAAGGACGACACCGCGCCGGTG
TTCCAGTTTCTCGCGCGGATCAGCAGCGCGAGCAAGCCGATCGTGGCTGCGGTGCCGGGCCTGGCGATCGGCGTCGGCGT
AACGATGCTGCTGCACTGCGACCTGGTCTACGCGGCCGACACCGCGACGTTCTCGCTGCCGTTCACGCAGCTCGGGCTGT
GCCCGGAAGCTGCGTCGAGCGTGCTGCTGCCGCGTCTGGCCGGCCATCAGGTGGCGGCCGAGAAGCTGCTGCTCGGCGAG
GCGTTCGACGCGCTGGAAGCGCACCGGATCGGCATCGTCAACCGCGTGCTGCCGGCGGCCGAGCTCGACGCGTTCGCGGC
GAAGCAGGCGGCGAAGCTCGCGGCACTGCCGGCGGCATCGCTGCGCGTGACGAAGGCGCTGCTCAAGGATACGGGCGGTG
TGGCGACGCAGGCGCGGATGGCCGAGGAAGCGGGCCACTTCGCCGCGATGCTGCGCGCACCGGAAGCGCGCGAGGCGATG
ACGGCGTTCTTCGAGAAGCGCAAGCCGGATTTCCGTCAGTTCGACTGA

Upstream 100 bases:

>100_bases
CGCCTGTAAGCGGAGCGGGACAAGAGCGACGACGGTGCGCGGGCCACGAGCTCGCGCACCGTTTTTTCATTCCGATAGAG
GAAAGTCAAAGGAGACGGTT

Downstream 100 bases:

>100_bases
CGGAGCGGGGCACGCCGGCGGCGGACCGCCGGCGCACTCCCGCGAGCGCGATCAGGCCGTGCCGTAGTCGACGTGGCCGG
TTTCGCGGCAGAAGCTGACG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MAEIQVERADGVMTITIARPAKKNALTAAMYQTMADALAEAQEDKAVRVVLLRGGDGNFTAGNDLEDFLKSPPKDDTAPV
FQFLARISSASKPIVAAVPGLAIGVGVTMLLHCDLVYAADTATFSLPFTQLGLCPEAASSVLLPRLAGHQVAAEKLLLGE
AFDALEAHRIGIVNRVLPAAELDAFAAKQAAKLAALPAASLRVTKALLKDTGGVATQARMAEEAGHFAAMLRAPEAREAM
TAFFEKRKPDFRQFD

Sequences:

>Translated_255_residues
MAEIQVERADGVMTITIARPAKKNALTAAMYQTMADALAEAQEDKAVRVVLLRGGDGNFTAGNDLEDFLKSPPKDDTAPV
FQFLARISSASKPIVAAVPGLAIGVGVTMLLHCDLVYAADTATFSLPFTQLGLCPEAASSVLLPRLAGHQVAAEKLLLGE
AFDALEAHRIGIVNRVLPAAELDAFAAKQAAKLAALPAASLRVTKALLKDTGGVATQARMAEEAGHFAAMLRAPEAREAM
TAFFEKRKPDFRQFD
>Mature_254_residues
AEIQVERADGVMTITIARPAKKNALTAAMYQTMADALAEAQEDKAVRVVLLRGGDGNFTAGNDLEDFLKSPPKDDTAPVF
QFLARISSASKPIVAAVPGLAIGVGVTMLLHCDLVYAADTATFSLPFTQLGLCPEAASSVLLPRLAGHQVAAEKLLLGEA
FDALEAHRIGIVNRVLPAAELDAFAAKQAAKLAALPAASLRVTKALLKDTGGVATQARMAEEAGHFAAMLRAPEAREAMT
AFFEKRKPDFRQFD

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI45643119, Length=249, Percent_Identity=32.1285140562249, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI260275230, Length=249, Percent_Identity=32.1285140562249, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI260274832, Length=249, Percent_Identity=32.1285140562249, Blast_Score=132, Evalue=2e-31,
Organism=Homo sapiens, GI194097323, Length=250, Percent_Identity=29.6, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI31542718, Length=252, Percent_Identity=30.5555555555556, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI4757968, Length=257, Percent_Identity=28.7937743190661, Blast_Score=101, Evalue=5e-22,
Organism=Homo sapiens, GI49355787, Length=257, Percent_Identity=28.7937743190661, Blast_Score=101, Evalue=5e-22,
Organism=Homo sapiens, GI25453481, Length=257, Percent_Identity=28.0155642023346, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI51538573, Length=257, Percent_Identity=28.0155642023346, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI4757966, Length=215, Percent_Identity=31.1627906976744, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI51538581, Length=215, Percent_Identity=31.1627906976744, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI221136756, Length=257, Percent_Identity=25.2918287937743, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI221136753, Length=257, Percent_Identity=25.2918287937743, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI221307494, Length=257, Percent_Identity=25.2918287937743, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI70995211, Length=186, Percent_Identity=30.1075268817204, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI68989263, Length=258, Percent_Identity=28.6821705426357, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI150378541, Length=260, Percent_Identity=30.7692307692308, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI37594469, Length=202, Percent_Identity=27.7227722772277, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI116235487, Length=215, Percent_Identity=27.906976744186, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI37594471, Length=187, Percent_Identity=27.807486631016, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4502327, Length=254, Percent_Identity=28.740157480315, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI62530384, Length=237, Percent_Identity=24.4725738396624, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1787659, Length=255, Percent_Identity=33.7254901960784, Blast_Score=139, Evalue=1e-34,
Organism=Escherichia coli, GI1788597, Length=264, Percent_Identity=34.0909090909091, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1787660, Length=250, Percent_Identity=33.2, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI221142681, Length=256, Percent_Identity=31.25, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87082183, Length=257, Percent_Identity=28.7937743190661, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790281, Length=256, Percent_Identity=26.5625, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788682, Length=193, Percent_Identity=29.5336787564767, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=253, Percent_Identity=36.7588932806324, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17535521, Length=253, Percent_Identity=32.8063241106719, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17554946, Length=256, Percent_Identity=31.25, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI25145438, Length=244, Percent_Identity=29.9180327868852, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17560910, Length=197, Percent_Identity=30.9644670050761, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17534483, Length=204, Percent_Identity=27.9411764705882, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17540714, Length=189, Percent_Identity=29.1005291005291, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25144276, Length=215, Percent_Identity=31.6279069767442, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=242, Percent_Identity=24.3801652892562, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17508951, Length=215, Percent_Identity=31.6279069767442, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17508953, Length=215, Percent_Identity=31.6279069767442, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17558304, Length=185, Percent_Identity=30.2702702702703, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=197, Percent_Identity=27.4111675126904, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24654903, Length=250, Percent_Identity=33.6, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI20129971, Length=241, Percent_Identity=30.2904564315353, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24653477, Length=241, Percent_Identity=30.2904564315353, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24653139, Length=264, Percent_Identity=33.3333333333333, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI19922422, Length=262, Percent_Identity=25.9541984732824, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI21357171, Length=221, Percent_Identity=27.1493212669683, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24650670, Length=206, Percent_Identity=27.6699029126214, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19920382, Length=183, Percent_Identity=27.3224043715847, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI28571729, Length=139, Percent_Identity=30.2158273381295, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI28571730, Length=139, Percent_Identity=30.2158273381295, Blast_Score=70, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 26986; Mature: 26855

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEIQVERADGVMTITIARPAKKNALTAAMYQTMADALAEAQEDKAVRVVLLRGGDGNFT
CCCEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
AGNDLEDFLKSPPKDDTAPVFQFLARISSASKPIVAAVPGLAIGVGVTMLLHCDLVYAAD
CCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHEEEC
TATFSLPFTQLGLCPEAASSVLLPRLAGHQVAAEKLLLGEAFDALEAHRIGIVNRVLPAA
CCEEECCHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ELDAFAAKQAAKLAALPAASLRVTKALLKDTGGVATQARMAEEAGHFAAMLRAPEAREAM
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
TAFFEKRKPDFRQFD
HHHHHHCCCCCCCCC
>Mature Secondary Structure 
AEIQVERADGVMTITIARPAKKNALTAAMYQTMADALAEAQEDKAVRVVLLRGGDGNFT
CCEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
AGNDLEDFLKSPPKDDTAPVFQFLARISSASKPIVAAVPGLAIGVGVTMLLHCDLVYAAD
CCCCHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHEEEC
TATFSLPFTQLGLCPEAASSVLLPRLAGHQVAAEKLLLGEAFDALEAHRIGIVNRVLPAA
CCEEECCHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ELDAFAAKQAAKLAALPAASLRVTKALLKDTGGVATQARMAEEAGHFAAMLRAPEAREAM
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
TAFFEKRKPDFRQFD
HHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]