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The map label for this gene is lipA [H]

Identifier: 78066606

GI number: 78066606

Start: 2182249

End: 2183214

Strand: Reverse

Name: lipA [H]

Synonym: Bcep18194_A5137

Alternate gene names: 78066606

Gene position: 2183214-2182249 (Counterclockwise)

Preceding gene: 78066607

Following gene: 78066597

Centisome position: 59.1

GC content: 71.22

Gene sequence:

>966_bases
ATGGCCGCCGCGCTCGAACGACCCAGCCTCACGGCGCTCGGCCAGCCGGGCGCGAGAAGCCGCGACAAGCTCGCGCGCAT
TCCGGTGCGCGTCGAGCCCGCGGCCGGTGCGGCGTTGCCGAAGCCGCCGTGGCTGCGCGCGCGGCCGATGATGAGCGCGG
CGGTTGCCGATATGGCGGCCGTGCTGCGCGACCATCGGCTGCATTCCGTCTGCGAGGAGGCGATGTGCCCGAACATCGGC
GAATGCTTCGCGCAGCGCACGGCGACCTTCATGATCATGGGCGGGCTGTGTACGCGGCGTTGCCCGTTCTGCGACGTCGC
GCACGGCCGTCCCGAACCGCTCGATCCGGACGAGCCCGCGCGACTGGCCGATGCGGCTGCGGCGCTCGGGCTGCGCTATG
TCGTGATCACGTCGGTCGATCGCGACGACCTGCGCGACGGCGGCGCCGCGCATTTTGCCGCTTGCATCGCGGCGGTGCGC
GCGAGCGTGCCGGGCATCGGCGTCGAAGTGCTGACACCGGACTTCCGCGGTCGCGTGGCCCGCGCGCTCGATGCGCTGTC
GATGGCCTGGCCCGACGTGTTCAACCACAACATCGAGACGGTGCCGTCGCTGTATCGCGCGGCGCGGCCGGGCGCCGACT
ATCGCGGCTCGCTCGAACTGCTCGCGCAGGCGAAGCGGGCGCGGCCGGGGCTGGTGACGAAATCGGGGTTGATGCTCGGC
CTTGGCGAGTGCGACGACGAAGTGCGCGACACGCTGCGCGACCTGCGCGCGCATGACGTCGACGTGCTGACGCTCGGCCA
GTATCTCGCGCCGTCCGCGCATCATCTGCCGGTGCGACGCTACGTGAGCCCGGACGCATTCGCCGCGTGGCGCGACGAAG
CGCTATTGCTCGGTTTCAGGGAAGTCGTCGCCGGCCCGCTCGTGCGGTCGTCGTATCACGCGGCCGACGTGCTTGAGGAG
AAGTAG

Upstream 100 bases:

>100_bases
GCGTGCGTGCCGAGGTGATCGCCGGCAGCGGGCACATGGTGCAGATGGAGGCCGCCGCCGACGTGAACCGCCTGATCGTC
GCGTTTCTCGGAGACTGACG

Downstream 100 bases:

>100_bases
CGGGCAACGCGTCAGGCGGACGTGGACACGGCGGCATGCGCACGGCTGACGCGGCGCAGGTACAGCACGAGCCCGGCGCC
CGCGAGCATCAGGCTGATCG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MAAALERPSLTALGQPGARSRDKLARIPVRVEPAAGAALPKPPWLRARPMMSAAVADMAAVLRDHRLHSVCEEAMCPNIG
ECFAQRTATFMIMGGLCTRRCPFCDVAHGRPEPLDPDEPARLADAAAALGLRYVVITSVDRDDLRDGGAAHFAACIAAVR
ASVPGIGVEVLTPDFRGRVARALDALSMAWPDVFNHNIETVPSLYRAARPGADYRGSLELLAQAKRARPGLVTKSGLMLG
LGECDDEVRDTLRDLRAHDVDVLTLGQYLAPSAHHLPVRRYVSPDAFAAWRDEALLLGFREVVAGPLVRSSYHAADVLEE
K

Sequences:

>Translated_321_residues
MAAALERPSLTALGQPGARSRDKLARIPVRVEPAAGAALPKPPWLRARPMMSAAVADMAAVLRDHRLHSVCEEAMCPNIG
ECFAQRTATFMIMGGLCTRRCPFCDVAHGRPEPLDPDEPARLADAAAALGLRYVVITSVDRDDLRDGGAAHFAACIAAVR
ASVPGIGVEVLTPDFRGRVARALDALSMAWPDVFNHNIETVPSLYRAARPGADYRGSLELLAQAKRARPGLVTKSGLMLG
LGECDDEVRDTLRDLRAHDVDVLTLGQYLAPSAHHLPVRRYVSPDAFAAWRDEALLLGFREVVAGPLVRSSYHAADVLEE
K
>Mature_320_residues
AAALERPSLTALGQPGARSRDKLARIPVRVEPAAGAALPKPPWLRARPMMSAAVADMAAVLRDHRLHSVCEEAMCPNIGE
CFAQRTATFMIMGGLCTRRCPFCDVAHGRPEPLDPDEPARLADAAAALGLRYVVITSVDRDDLRDGGAAHFAACIAAVRA
SVPGIGVEVLTPDFRGRVARALDALSMAWPDVFNHNIETVPSLYRAARPGADYRGSLELLAQAKRARPGLVTKSGLMLGL
GECDDEVRDTLRDLRAHDVDVLTLGQYLAPSAHHLPVRRYVSPDAFAAWRDEALLLGFREVVAGPLVRSSYHAADVLEEK

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=290, Percent_Identity=46.8965517241379, Blast_Score=267, Evalue=1e-71,
Organism=Homo sapiens, GI37577164, Length=251, Percent_Identity=47.808764940239, Blast_Score=230, Evalue=2e-60,
Organism=Escherichia coli, GI1786846, Length=298, Percent_Identity=59.3959731543624, Blast_Score=361, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI32564533, Length=285, Percent_Identity=44.9122807017544, Blast_Score=240, Evalue=8e-64,
Organism=Saccharomyces cerevisiae, GI6324770, Length=287, Percent_Identity=43.5540069686411, Blast_Score=239, Evalue=3e-64,
Organism=Drosophila melanogaster, GI221513272, Length=297, Percent_Identity=46.1279461279461, Blast_Score=266, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 34634; Mature: 34503

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAALERPSLTALGQPGARSRDKLARIPVRVEPAAGAALPKPPWLRARPMMSAAVADMAA
CCCCCCCCCCCCCCCCCCCCCHHHHHCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHH
VLRDHRLHSVCEEAMCPNIGECFAQRTATFMIMGGLCTRRCPFCDVAHGRPEPLDPDEPA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEECCHHHCCCCCCCCCCCCCCCCCCCCHH
RLADAAAALGLRYVVITSVDRDDLRDGGAAHFAACIAAVRASVPGIGVEVLTPDFRGRVA
HHHHHHHHHHEEEEEEECCCHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHH
RALDALSMAWPDVFNHNIETVPSLYRAARPGADYRGSLELLAQAKRARPGLVTKSGLMLG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEECCCCEEE
LGECDDEVRDTLRDLRAHDVDVLTLGQYLAPSAHHLPVRRYVSPDAFAAWRDEALLLGFR
CCCCCHHHHHHHHHHHHCCCCCEEHHHHHCCCCCCCCHHHHCCCCHHHHHCCHHHHHHHH
EVVAGPLVRSSYHAADVLEEK
HHHHCHHHHCCHHHHHHHCCC
>Mature Secondary Structure 
AAALERPSLTALGQPGARSRDKLARIPVRVEPAAGAALPKPPWLRARPMMSAAVADMAA
CCCCCCCCCCCCCCCCCCCCHHHHHCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHH
VLRDHRLHSVCEEAMCPNIGECFAQRTATFMIMGGLCTRRCPFCDVAHGRPEPLDPDEPA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHEECCHHHCCCCCCCCCCCCCCCCCCCCHH
RLADAAAALGLRYVVITSVDRDDLRDGGAAHFAACIAAVRASVPGIGVEVLTPDFRGRVA
HHHHHHHHHHEEEEEEECCCHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHH
RALDALSMAWPDVFNHNIETVPSLYRAARPGADYRGSLELLAQAKRARPGLVTKSGLMLG
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEECCCCEEE
LGECDDEVRDTLRDLRAHDVDVLTLGQYLAPSAHHLPVRRYVSPDAFAAWRDEALLLGFR
CCCCCHHHHHHHHHHHHCCCCCEEHHHHHCCCCCCCCHHHHCCCCHHHHHCCHHHHHHHH
EVVAGPLVRSSYHAADVLEEK
HHHHCHHHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA