Click here to switch to the map view.

The map label for this gene is norM [H]

Identifier: 78065676

GI number: 78065676

Start: 1140115

End: 1141503

Strand: Reverse

Name: norM [H]

Synonym: Bcep18194_A4204

Alternate gene names: 78065676

Gene position: 1141503-1140115 (Counterclockwise)

Preceding gene: 78065677

Following gene: 78065675

Centisome position: 30.9

GC content: 70.12

Gene sequence:

>1389_bases
ATGTCGCATTCCGGTCTTACGCGGACGGCCGCCGCGCCGCCGTCCCTGTCCAGTCACGCCGCCGATACCGCGCGCCTCGC
CGCGCCGCTCGCGATCGCGCAGCTCTCGCAGATGGCGATGAGCGTCACCGATACCGTGCTGCTCGGCTCGCTCGGCCCCG
ATTCGCTCGCGGCGGGCGGGCTCGGCGCGAACTTCTTCTTCGTCATCGTGACCGTGCTGCAAGGCGTGCTGTCGTCCGTC
AGCGTCAGCGTCGCGCACGCGCGCGGCGCCAAGGCCGACGAGCGCGTGCCCCACATCTACTGGACCGGTTTCGCGCTGTC
CGTACTGCTCGCGATTCCGGCCATCATCGCGCTGTCGCTGTCCGAACCGATCCTGCTGATGTTCCACGAGCCGCCATTGC
TCGCGCATCACGTCGGCGAATACACCGGCATCCTGCGCTTCGCCGCGCTCGGCAGCCTGATCGGCGTCGGGCTGATGCGC
GCATTCCTGCCGGCGATCGGCGCGGCGCGCCGGCTGCTGTGGGTGTCGATCGGCGGCGTCGGCGTCAACGGCGTGCTGAA
CTACGGGCTGATCCACGGCGCGTTCGGGCTGCCGCGCCTCGGCTTCCTCGGTTCGGCGGTCGCAACGACGATCACGATCT
GGCTTACCGCACTCGCACTGATCTGGCTGCTGCACGGCCGGCAACGCTTCCGTCATTTCGTCACGGCCGCGCGCCCGAAG
CTGCCGATGATGGGCGAACTGATCGGCATCGGCTGGCCCGTTGCGATCACGTACGGCGTCGAATCGACGCTGTTCCTCGC
GACCGGCCTGACGATCGGCGTGCTCGGCGCGACGTCGCTGGCCGCCCACCAGATCGCGCTGAACGTCGCGTCGGTGGCGT
TCATGGTGCCGCTCGCGATCGGCCAGGCCGCCAACGTGCGGGTCGGTTACTGGGTCGGCGCAGGGGCGCCGGTCGCCGCG
CGGCATGCGGGCTTCGTCGCACTCGGCCTCGGGGTCGCGTTCATGACGCTGTCGGGCATCGTGCTGATCGTCGCACCGCA
TGCGATCGTCGGCCTGTACCTGAACCTCGACGATCCGGCCAACGCGGCCACCGTGTCGCTCGCCGCGTCGCTGCTCGGCA
TCGCCGCGGTGTTCCAGATCGTCGACGGCGCGCAGACCGTTGCGTCGGGTGCGCTGCGCGGCCTGAAGGACACGCGCATC
CCGATGCTCGCGGCGACCCTCGGCTACTGGGGCATCGGTTTTCCGACCGGCTACTGGTTCGCGTTCCACGTGGGCCTCGG
TGCCCGCGGCCTCTGGTGGGGCCTCGCGGCCGGCCTCGCCAGCGTGGCCGTGCTGATGGCATGGCGCTTCCATCTGAAGA
GCGCGTCGTTCGTGACGTCGCCGCACTGA

Upstream 100 bases:

>100_bases
GGCGCGCGCGTTCGACACGGTTCGAATAAAGCCGTAAGCTAGCCGTCTCTCAAAATAAATACATATAGCGCGGGCCCGCA
CCCGCGCGAGCGCCCATTCC

Downstream 100 bases:

>100_bases
GCGCGACAAGACGCGAAATCCGGGCCGGCGCTATGCTAGTCGGCTGAAGACATCAAGACGCCGCGCGAGCGGCGCGACTG
CCTCACGATACGTCTGATTA

Product: multi anti extrusion protein MatE

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSHSGLTRTAAAPPSLSSHAADTARLAAPLAIAQLSQMAMSVTDTVLLGSLGPDSLAAGGLGANFFFVIVTVLQGVLSSV
SVSVAHARGAKADERVPHIYWTGFALSVLLAIPAIIALSLSEPILLMFHEPPLLAHHVGEYTGILRFAALGSLIGVGLMR
AFLPAIGAARRLLWVSIGGVGVNGVLNYGLIHGAFGLPRLGFLGSAVATTITIWLTALALIWLLHGRQRFRHFVTAARPK
LPMMGELIGIGWPVAITYGVESTLFLATGLTIGVLGATSLAAHQIALNVASVAFMVPLAIGQAANVRVGYWVGAGAPVAA
RHAGFVALGLGVAFMTLSGIVLIVAPHAIVGLYLNLDDPANAATVSLAASLLGIAAVFQIVDGAQTVASGALRGLKDTRI
PMLAATLGYWGIGFPTGYWFAFHVGLGARGLWWGLAAGLASVAVLMAWRFHLKSASFVTSPH

Sequences:

>Translated_462_residues
MSHSGLTRTAAAPPSLSSHAADTARLAAPLAIAQLSQMAMSVTDTVLLGSLGPDSLAAGGLGANFFFVIVTVLQGVLSSV
SVSVAHARGAKADERVPHIYWTGFALSVLLAIPAIIALSLSEPILLMFHEPPLLAHHVGEYTGILRFAALGSLIGVGLMR
AFLPAIGAARRLLWVSIGGVGVNGVLNYGLIHGAFGLPRLGFLGSAVATTITIWLTALALIWLLHGRQRFRHFVTAARPK
LPMMGELIGIGWPVAITYGVESTLFLATGLTIGVLGATSLAAHQIALNVASVAFMVPLAIGQAANVRVGYWVGAGAPVAA
RHAGFVALGLGVAFMTLSGIVLIVAPHAIVGLYLNLDDPANAATVSLAASLLGIAAVFQIVDGAQTVASGALRGLKDTRI
PMLAATLGYWGIGFPTGYWFAFHVGLGARGLWWGLAAGLASVAVLMAWRFHLKSASFVTSPH
>Mature_461_residues
SHSGLTRTAAAPPSLSSHAADTARLAAPLAIAQLSQMAMSVTDTVLLGSLGPDSLAAGGLGANFFFVIVTVLQGVLSSVS
VSVAHARGAKADERVPHIYWTGFALSVLLAIPAIIALSLSEPILLMFHEPPLLAHHVGEYTGILRFAALGSLIGVGLMRA
FLPAIGAARRLLWVSIGGVGVNGVLNYGLIHGAFGLPRLGFLGSAVATTITIWLTALALIWLLHGRQRFRHFVTAARPKL
PMMGELIGIGWPVAITYGVESTLFLATGLTIGVLGATSLAAHQIALNVASVAFMVPLAIGQAANVRVGYWVGAGAPVAAR
HAGFVALGLGVAFMTLSGIVLIVAPHAIVGLYLNLDDPANAATVSLAASLLGIAAVFQIVDGAQTVASGALRGLKDTRIP
MLAATLGYWGIGFPTGYWFAFHVGLGARGLWWGLAAGLASVAVLMAWRFHLKSASFVTSPH

Specific function: Multidrug efflux pump. Confers probably resistance to the cationic peptide polymyxin B (PMB) [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Homo sapiens, GI22907060, Length=427, Percent_Identity=26.6978922716628, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI153792564, Length=418, Percent_Identity=24.4019138755981, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI38261960, Length=450, Percent_Identity=22.8888888888889, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI48994890, Length=435, Percent_Identity=32.183908045977, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI226510954, Length=447, Percent_Identity=27.0693512304251, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 47676; Mature: 47545

Theoretical pI: Translated: 10.62; Mature: 10.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHSGLTRTAAAPPSLSSHAADTARLAAPLAIAQLSQMAMSVTDTVLLGSLGPDSLAAGG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC
LGANFFFVIVTVLQGVLSSVSVSVAHARGAKADERVPHIYWTGFALSVLLAIPAIIALSL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHC
SEPILLMFHEPPLLAHHVGEYTGILRFAALGSLIGVGLMRAFLPAIGAARRLLWVSIGGV
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCC
GVNGVLNYGLIHGAFGLPRLGFLGSAVATTITIWLTALALIWLLHGRQRFRHFVTAARPK
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LPMMGELIGIGWPVAITYGVESTLFLATGLTIGVLGATSLAAHQIALNVASVAFMVPLAI
CCCHHHHHCCCCCEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GQAANVRVGYWVGAGAPVAARHAGFVALGLGVAFMTLSGIVLIVAPHAIVGLYLNLDDPA
CCCCCEEEEEEECCCCCHHHHHCCHHHHHHHHHHHHHCCEEEEEECHHHEEEEEECCCCC
NAATVSLAASLLGIAAVFQIVDGAQTVASGALRGLKDTRIPMLAATLGYWGIGFPTGYWF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHE
AFHVGLGARGLWWGLAAGLASVAVLMAWRFHLKSASFVTSPH
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SHSGLTRTAAAPPSLSSHAADTARLAAPLAIAQLSQMAMSVTDTVLLGSLGPDSLAAGG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC
LGANFFFVIVTVLQGVLSSVSVSVAHARGAKADERVPHIYWTGFALSVLLAIPAIIALSL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHC
SEPILLMFHEPPLLAHHVGEYTGILRFAALGSLIGVGLMRAFLPAIGAARRLLWVSIGGV
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCC
GVNGVLNYGLIHGAFGLPRLGFLGSAVATTITIWLTALALIWLLHGRQRFRHFVTAARPK
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LPMMGELIGIGWPVAITYGVESTLFLATGLTIGVLGATSLAAHQIALNVASVAFMVPLAI
CCCHHHHHCCCCCEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GQAANVRVGYWVGAGAPVAARHAGFVALGLGVAFMTLSGIVLIVAPHAIVGLYLNLDDPA
CCCCCEEEEEEECCCCCHHHHHCCHHHHHHHHHHHHHCCEEEEEECHHHEEEEEECCCCC
NAATVSLAASLLGIAAVFQIVDGAQTVASGALRGLKDTRIPMLAATLGYWGIGFPTGYWF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHE
AFHVGLGARGLWWGLAAGLASVAVLMAWRFHLKSASFVTSPH
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12399047 [H]