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The map label for this gene is ubiH [H]

Identifier: 78065256

GI number: 78065256

Start: 674771

End: 675958

Strand: Reverse

Name: ubiH [H]

Synonym: Bcep18194_A3780

Alternate gene names: 78065256

Gene position: 675958-674771 (Counterclockwise)

Preceding gene: 78065257

Following gene: 78065255

Centisome position: 18.3

GC content: 72.39

Gene sequence:

>1188_bases
ATGACGACCGCTTCCTCCCCGGCCACGCCGGACTACGACCTCGCCATCGTCGGCGCGGGCCCCGTCGGGCTCGCGCTCGC
CGGCTGGCTCGCGCGCCGCAGCGCCACGCAGCATGCATCGATCGCGCTGATCGATGCACGCGAACCGGCCGCGAGCGCGA
ACGATCCGCGCGCGATCGCGGTGTCGCACGGCAGCCGCGTGCTGCTCGACACGCTCGCATGGCCCGCCGACGCGACGCCG
ATCGAACATATCCACGTCTCGCAGCGCGGCCATTTCGGCCGCACGCTGATCGACCGCGACGAGCACGACGTCGCCGCGCT
CGGCTATGTCGTGCGCTACGGCTCGCTCATGCAGGCGCTCGCCGGCGCCGTGCGCGGCACGCGTGTCGACTGGCACACGT
CGACCACCGCGCGCGCGCCGCAGCAGGACGCCGACGGCGTGACGCTGACGCTCGACGGCCCGCAGGGCGATCGCACGCTG
CGCGCACGCGTCGTCATCAATGCCGAAGGCGGGCTGTTCCACGAACAGCAGGCCGACGCCGGCAAACATCGCCGCGACTA
TGGACAGACCGCGCTCGTCGGCACGGTCACCGTATCGGCGCCGCGCCCGAACGTCGCGTGGGAACGCTTCACGCACGAAG
GCCCGCTCGCGCTGCTGCCGCTCGGCGGGCCGCGCCAGGCCGAGTACGCGCTCGTCTGGTGCTGCACGCCCGACGAAGCG
GCACGCCGCGCCGCGCTGCCCGACGACGCGTTCCTGCGCGAGCTCGGCAGCGCGTTCGGCGAACGCATGGGCGACTTCGT
CGCGATTGCGGGCCGCGCGTCGTTCCCGCTCGGCCTGAACGCCGCTCAAACGCTCGTCAACGGCCGCGTCGCGATCGTCG
GCAATGCCGCGCAAACCCTGCACCCCGTTGCGGGCCAGGGGCTCAACCTCGGGCTGCGCGATGCGCATACGCTCGTCGAT
ACGCTGTCCGCGCAAGGCTTCGAAGCGACCGCGCTCGCCTCCTTCAACGCGCGCCGCGCACTCGACCGGCGCTTTACGAT
CGGCGCGACCGACACGCTCGCGCGCCTGTTCACGATCGACTCGGGCCCGCTCCCGCTGCTGCGCGGCGCCGCGCTCACCG
CGCTCGAGTTCGTGCCGCCGCTCAAGAAAGTGATCGCGCGCCAGATGATGTTCGGCCAGCGCAACTGA

Upstream 100 bases:

>100_bases
ACGCGCGGCGTGCCCGTCGCAGCGGACGAGATCGAAGCGCTGATGCGCGCAGGCGCATGACCGGCTGCAGCCGACCGTTA
CCACCGTTTCACGAATCAAG

Downstream 100 bases:

>100_bases
CGCGCGGCGCGCACAGTCGCGCCACTCAAACCGGGTCAAATCGGCGGGGCATGGGAATACGGTAAAATGCGCGTTTCCGT
CTGCCCTTTCCCTGCCCGCG

Product: hypothetical protein

Products: H2O; 2-octaprenyl-6-methoxy-1,4-benzoquinone [C]

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MTTASSPATPDYDLAIVGAGPVGLALAGWLARRSATQHASIALIDAREPAASANDPRAIAVSHGSRVLLDTLAWPADATP
IEHIHVSQRGHFGRTLIDRDEHDVAALGYVVRYGSLMQALAGAVRGTRVDWHTSTTARAPQQDADGVTLTLDGPQGDRTL
RARVVINAEGGLFHEQQADAGKHRRDYGQTALVGTVTVSAPRPNVAWERFTHEGPLALLPLGGPRQAEYALVWCCTPDEA
ARRAALPDDAFLRELGSAFGERMGDFVAIAGRASFPLGLNAAQTLVNGRVAIVGNAAQTLHPVAGQGLNLGLRDAHTLVD
TLSAQGFEATALASFNARRALDRRFTIGATDTLARLFTIDSGPLPLLRGAALTALEFVPPLKKVIARQMMFGQRN

Sequences:

>Translated_395_residues
MTTASSPATPDYDLAIVGAGPVGLALAGWLARRSATQHASIALIDAREPAASANDPRAIAVSHGSRVLLDTLAWPADATP
IEHIHVSQRGHFGRTLIDRDEHDVAALGYVVRYGSLMQALAGAVRGTRVDWHTSTTARAPQQDADGVTLTLDGPQGDRTL
RARVVINAEGGLFHEQQADAGKHRRDYGQTALVGTVTVSAPRPNVAWERFTHEGPLALLPLGGPRQAEYALVWCCTPDEA
ARRAALPDDAFLRELGSAFGERMGDFVAIAGRASFPLGLNAAQTLVNGRVAIVGNAAQTLHPVAGQGLNLGLRDAHTLVD
TLSAQGFEATALASFNARRALDRRFTIGATDTLARLFTIDSGPLPLLRGAALTALEFVPPLKKVIARQMMFGQRN
>Mature_394_residues
TTASSPATPDYDLAIVGAGPVGLALAGWLARRSATQHASIALIDAREPAASANDPRAIAVSHGSRVLLDTLAWPADATPI
EHIHVSQRGHFGRTLIDRDEHDVAALGYVVRYGSLMQALAGAVRGTRVDWHTSTTARAPQQDADGVTLTLDGPQGDRTLR
ARVVINAEGGLFHEQQADAGKHRRDYGQTALVGTVTVSAPRPNVAWERFTHEGPLALLPLGGPRQAEYALVWCCTPDEAA
RRAALPDDAFLRELGSAFGERMGDFVAIAGRASFPLGLNAAQTLVNGRVAIVGNAAQTLHPVAGQGLNLGLRDAHTLVDT
LSAQGFEATALASFNARRALDRRFTIGATDTLARLFTIDSGPLPLLRGAALTALEFVPPLKKVIARQMMFGQRN

Specific function: Oxygenase that introduces the hydroxyl group at carbon four of 2-octaprenyl-6-methoxyphenol resulting in the formation of 2-octaprenyl-6-methoxy-1,4-benzoquinone [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=413, Percent_Identity=29.0556900726392, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI32967307, Length=355, Percent_Identity=30.1408450704225, Blast_Score=119, Evalue=4e-27,
Organism=Escherichia coli, GI1789274, Length=395, Percent_Identity=35.1898734177215, Blast_Score=177, Evalue=8e-46,
Organism=Escherichia coli, GI1789273, Length=397, Percent_Identity=31.4861460957179, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1786883, Length=395, Percent_Identity=31.8987341772152, Blast_Score=117, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321694, Length=120, Percent_Identity=37.5, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI19920760, Length=441, Percent_Identity=26.3038548752834, Blast_Score=95, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971
- InterPro:   IPR011295 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 41891; Mature: 41760

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTASSPATPDYDLAIVGAGPVGLALAGWLARRSATQHASIALIDAREPAASANDPRAIA
CCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCEEE
VSHGSRVLLDTLAWPADATPIEHIHVSQRGHFGRTLIDRDEHDVAALGYVVRYGSLMQAL
EECCCEEEEHHHCCCCCCCCHHEEEECCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHH
AGAVRGTRVDWHTSTTARAPQQDADGVTLTLDGPQGDRTLRARVVINAEGGLFHEQQADA
HHHHCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEEEECCCCCCCHHHHHC
GKHRRDYGQTALVGTVTVSAPRPNVAWERFTHEGPLALLPLGGPRQAEYALVWCCTPDEA
HHHHHHCCCEEEEEEEEEECCCCCCHHHHHCCCCCEEEEECCCCCCCCEEEEEEECCHHH
ARRAALPDDAFLRELGSAFGERMGDFVAIAGRASFPLGLNAAQTLVNGRVAIVGNAAQTL
HHHCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHCCCEEEEECCHHHH
HPVAGQGLNLGLRDAHTLVDTLSAQGFEATALASFNARRALDRRFTIGATDTLARLFTID
CHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHCEEECCCHHHHHHHEEEC
SGPLPLLRGAALTALEFVPPLKKVIARQMMFGQRN
CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTASSPATPDYDLAIVGAGPVGLALAGWLARRSATQHASIALIDAREPAASANDPRAIA
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCEEE
VSHGSRVLLDTLAWPADATPIEHIHVSQRGHFGRTLIDRDEHDVAALGYVVRYGSLMQAL
EECCCEEEEHHHCCCCCCCCHHEEEECCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHH
AGAVRGTRVDWHTSTTARAPQQDADGVTLTLDGPQGDRTLRARVVINAEGGLFHEQQADA
HHHHCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEEEECCCCCCCHHHHHC
GKHRRDYGQTALVGTVTVSAPRPNVAWERFTHEGPLALLPLGGPRQAEYALVWCCTPDEA
HHHHHHCCCEEEEEEEEEECCCCCCHHHHHCCCCCEEEEECCCCCCCCEEEEEEECCHHH
ARRAALPDDAFLRELGSAFGERMGDFVAIAGRASFPLGLNAAQTLVNGRVAIVGNAAQTL
HHHCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHCCCEEEEECCHHHH
HPVAGQGLNLGLRDAHTLVDTLSAQGFEATALASFNARRALDRRFTIGATDTLARLFTID
CHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHCEEECCCHHHHHHHEEEC
SGPLPLLRGAALTALEFVPPLKKVIARQMMFGQRN
CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-octaprenyl-6-methoxyphenol; O2 [C]

Specific reaction: 2-octaprenyl-6-methoxyphenol + O2 = H2O + 2-octaprenyl-6-methoxy-1,4-benzoquinone [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1339425; 9278503 [H]