Click here to switch to the map view.

The map label for this gene is dusB [H]

Identifier: 78065255

GI number: 78065255

Start: 673579

End: 674646

Strand: Reverse

Name: dusB [H]

Synonym: Bcep18194_A3779

Alternate gene names: 78065255

Gene position: 674646-673579 (Counterclockwise)

Preceding gene: 78065256

Following gene: 78065254

Centisome position: 18.26

GC content: 65.45

Gene sequence:

>1068_bases
ATGCCCGTTATCGGCCCTCACGTATTGCGTAACAACCTGTTCGTCGCCCCGATGGCCGGCGTGACGGATCGTCCGTTCCG
CCAGCTCTGCAAGCGGCTCGGGGCCGGTTACGCCGTGTCCGAGATGGTCGCGTCCAACGCGCAGCTGTGGAAAAGCGCGA
AGACGATGCGGCGTGCGAACCACGAAGGCGAGGTCGAGCCGATCGCGGTCCAGATCGCGGGCGCCGATCCGGCGATGATG
GCCGAAGCGGCCCGCTACAACGTCGACAACGGCGCGCAGATCATCGACATCAACATGGGCTGCCCGGCGAAAAAGGTCTG
CAACGTCGCGGCCGGCTCCGCGCTGCTGCAGAACGAACCGCTGGTGCAACGCATCGTCGAGGCCGTCGTCGCGGCGGTCG
GCACGGGGCCCGATGCGGTACCCGTCACGCTGAAGATCCGCACGGGCTGGGATCGCGAGCACAAGAACGCGATCACGGTC
GCGCGCCTCGCCGAAGCCGCCGGCATCTCGATGCTCACCGTGCACGGCCGCACGCGCGCCGACCTGTACCGCGGCGACGC
CGAATACGACACCATCGCGGCCGTGAAGGCGGCCGTACGGATTCCGGTGGTCGCGAACGGCGACATCACGTCGCCCGCGA
AGGCGAAGGCCGTGCTCGATGCAACCGGCGCCGACGCGCTGATGATCGGTCGTGCCGCGCAAGGTCGGCCGTGGCTGTTC
CGCGAAATCGATCATTTCCTGCAAACCGGCGAGCTGCTGCCCCCGCCGCTGATCGACGAGATCCAGCACGTGATGAACGA
ACACCTGGAAGACCACTACGCTTTCTATGGTGAATTCACGGGAGTCCGTACTGCGCGCAAGCACATCGGCTGGTACACTC
GCGGCCTTTCCGGTGCCAACGGGTTCCGGCACAGGATGAACACGCTCGATTCCTCCCGCGAGCAGCTCGCCGCCGTCAAT
GCATTCTTTGAGGCGCAAAAGGCGCTGTCGGACCACCTCGTCTACGTCGATGACGAAGAGGAAAACGGCCAGGGCGAGTC
GGACGACCATAACCAGTTAGCAGCATGA

Upstream 100 bases:

>100_bases
CTCAAACCGGGTCAAATCGGCGGGGCATGGGAATACGGTAAAATGCGCGTTTCCGTCTGCCCTTTCCCTGCCCGCGCCCG
CCGCGGGCGGTGCCATTGCG

Downstream 100 bases:

>100_bases
GCAAGCACAACATCGAACAATGTGTCCGCGAGAGCCTGGACGTGTATTTCCGGGATCTAGACGGCTCCAATCCGCACGAC
GTCTATGAAATGGTGATGTC

Product: dihydrouridine synthase TIM-barrel protein nifR3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MPVIGPHVLRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSAKTMRRANHEGEVEPIAVQIAGADPAMM
AEAARYNVDNGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVQRIVEAVVAAVGTGPDAVPVTLKIRTGWDREHKNAITV
ARLAEAAGISMLTVHGRTRADLYRGDAEYDTIAAVKAAVRIPVVANGDITSPAKAKAVLDATGADALMIGRAAQGRPWLF
REIDHFLQTGELLPPPLIDEIQHVMNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGANGFRHRMNTLDSSREQLAAVN
AFFEAQKALSDHLVYVDDEEENGQGESDDHNQLAA

Sequences:

>Translated_355_residues
MPVIGPHVLRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSAKTMRRANHEGEVEPIAVQIAGADPAMM
AEAARYNVDNGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVQRIVEAVVAAVGTGPDAVPVTLKIRTGWDREHKNAITV
ARLAEAAGISMLTVHGRTRADLYRGDAEYDTIAAVKAAVRIPVVANGDITSPAKAKAVLDATGADALMIGRAAQGRPWLF
REIDHFLQTGELLPPPLIDEIQHVMNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGANGFRHRMNTLDSSREQLAAVN
AFFEAQKALSDHLVYVDDEEENGQGESDDHNQLAA
>Mature_354_residues
PVIGPHVLRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSAKTMRRANHEGEVEPIAVQIAGADPAMMA
EAARYNVDNGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVQRIVEAVVAAVGTGPDAVPVTLKIRTGWDREHKNAITVA
RLAEAAGISMLTVHGRTRADLYRGDAEYDTIAAVKAAVRIPVVANGDITSPAKAKAVLDATGADALMIGRAAQGRPWLFR
EIDHFLQTGELLPPPLIDEIQHVMNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGANGFRHRMNTLDSSREQLAAVNA
FFEAQKALSDHLVYVDDEEENGQGESDDHNQLAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=238, Percent_Identity=32.7731092436975, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI239788462, Length=244, Percent_Identity=32.7868852459016, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI31742496, Length=228, Percent_Identity=33.7719298245614, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI40807366, Length=233, Percent_Identity=33.0472103004292, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI8923374, Length=253, Percent_Identity=27.2727272727273, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI1789660, Length=321, Percent_Identity=57.9439252336449, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1788462, Length=241, Percent_Identity=31.5352697095436, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI145693211, Length=243, Percent_Identity=27.5720164609054, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17543114, Length=237, Percent_Identity=29.535864978903, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI25144369, Length=219, Percent_Identity=33.7899543378995, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17507177, Length=246, Percent_Identity=30.4878048780488, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17510279, Length=254, Percent_Identity=29.1338582677165, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323433, Length=270, Percent_Identity=27.7777777777778, Blast_Score=92, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6323560, Length=240, Percent_Identity=29.5833333333333, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323437, Length=309, Percent_Identity=25.5663430420712, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24585320, Length=246, Percent_Identity=32.1138211382114, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24580595, Length=271, Percent_Identity=31.3653136531365, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19920448, Length=271, Percent_Identity=31.3653136531365, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19921524, Length=230, Percent_Identity=29.5652173913043, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI45549423, Length=255, Percent_Identity=28.6274509803922, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 38459; Mature: 38328

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVIGPHVLRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSAKTMRRAN
CCCCCCHHHHCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
HEGEVEPIAVQIAGADPAMMAEAARYNVDNGAQIIDINMGCPAKKVCNVAAGSALLQNEP
CCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCHHHCCCH
LVQRIVEAVVAAVGTGPDAVPVTLKIRTGWDREHKNAITVARLAEAAGISMLTVHGRTRA
HHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHCCHHHHHHHHHHCCCEEEEECCCCHH
DLYRGDAEYDTIAAVKAAVRIPVVANGDITSPAKAKAVLDATGADALMIGRAAQGRPWLF
HHHCCCCCHHHHHHHHHHHEEEEEECCCCCCCHHHHHEEECCCCCEEEECCCCCCCCHHH
REIDHFLQTGELLPPPLIDEIQHVMNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGAN
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GFRHRMNTLDSSREQLAAVNAFFEAQKALSDHLVYVDDEEENGQGESDDHNQLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PVIGPHVLRNNLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVASNAQLWKSAKTMRRAN
CCCCCHHHHCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
HEGEVEPIAVQIAGADPAMMAEAARYNVDNGAQIIDINMGCPAKKVCNVAAGSALLQNEP
CCCCCCEEEEEEECCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCHHHCCCH
LVQRIVEAVVAAVGTGPDAVPVTLKIRTGWDREHKNAITVARLAEAAGISMLTVHGRTRA
HHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHCCHHHHHHHHHHCCCEEEEECCCCHH
DLYRGDAEYDTIAAVKAAVRIPVVANGDITSPAKAKAVLDATGADALMIGRAAQGRPWLF
HHHCCCCCHHHHHHHHHHHEEEEEECCCCCCCHHHHHEEECCCCCEEEECCCCCCCCHHH
REIDHFLQTGELLPPPLIDEIQHVMNEHLEDHYAFYGEFTGVRTARKHIGWYTRGLSGAN
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GFRHRMNTLDSSREQLAAVNAFFEAQKALSDHLVYVDDEEENGQGESDDHNQLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]