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The map label for this gene is holA

Identifier: 78065218

GI number: 78065218

Start: 632554

End: 633648

Strand: Direct

Name: holA

Synonym: Bcep18194_A3742

Alternate gene names: 78065218

Gene position: 632554-633648 (Clockwise)

Preceding gene: 78065217

Following gene: 161702966

Centisome position: 17.12

GC content: 70.78

Gene sequence:

>1095_bases
ATGCAACTGCGACTTGATGCGCTGGAGCCGCACCTCGCGAAGGGGTTGGCCGGGCTCTATACCGTCTACGGCGACGAGCC
GCTGCTCGCGCAGGAAGCGTGCGACCGCATTCGTGCGGCCGCGCGCGCGGCCGGCTTCACCGAACGTTCGGTGCATACGG
TCGAGCGCGGCTTCGACTGGAGCGTGCTGCTCGGCGCAACCCAGGCGATGTCGCTGTTCGGCGAGCGTCAGCTGATCGAG
CTGCGCATTCCGTCGGGCAAGCCCGGCAAGGAAGGCGCCGATGCGCTGAAGACGCTCGCGGCCACGCCGAACCCCGACGC
GCTGATGCTCGTCACGCTGCCGCGTCTCGACGCGGCCACGCAGAAATCCGCGTGGTTCACGGCACTGCAGAACGGCGGCG
TCGCGCTGAAGATCGATCCGGTCGACCGCGCGCAGCTGCCGAACTGGATCGGCCAGCGTCTCGCGCTGCAGGGCCAGCGC
GCCGCGCCCGGCGACGACGGGCGGCGCGCGCTGCAGTTCATCGCCGAGCGCGTCGAAGGCAACCTGCTTGCCGCCCACCA
GGAAATCCAGAAGCTCGGGCTGCTGTATCCGCAGGGCGCGCTGTCGTTCGAGCAGGTGCACGACGCGGTGCTGAACGTCG
CGCGTTACGACGTCTTCAAGCTGAACGAAGCGATGCTCGCCGGCGACGCCGCGCGGCTCGCGCGGATGATCGACGGGCTG
AAGGGCGAGGGCGAGGCGATCGTGCTCGTGATGTGGGCCGTCGTCGAGGAATTGCGCACACTGCTGCGGATCAAGCGCGG
CACGACGGCGGGCAAGCCGCTCGCGACGCTGCTGCGCGAGAACCGCGTATGGGGGCCGCGCGAGCGGCTGATCGGCCCCG
CGCTGAATCGCGTGTCCGAAGCCGTGCTCGAGAGGGCGCTTGCGTTTGCCGCGAAGCTCGACCGTCAGGTCAAGGGGTTG
ACGGCCGTCGCGCCCGGCCGCCGCACGCAGGACGAACCGCCGCCCGATCCGTGGGACGGGCTGTTCCAGCTCGCGATGAC
GGTGGCCGGTGCCCGCGGCGAGCGACCGCCGACCGCGGGGCGCGTGCGACGCTAA

Upstream 100 bases:

>100_bases
CGTCCATTCGCTGACGCCGGCGCCGGAAGACGTGGTACCGGGCGTCGCACCGCGCGCGCCGTTGCCGCCGCCGCCGCTCT
GATCGACGGCGCACGTATCG

Downstream 100 bases:

>100_bases
GTCCCGTTCGGGCCTTCAGCGCGGGCTGCGCAACCCGCTTACAATGTTTCGATAGTTGGCGCGGCCCTCCGGCCGCCGCC
GCTCACGCCTCCCCACGAAT

Product: DNA polymerase III subunit delta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MQLRLDALEPHLAKGLAGLYTVYGDEPLLAQEACDRIRAAARAAGFTERSVHTVERGFDWSVLLGATQAMSLFGERQLIE
LRIPSGKPGKEGADALKTLAATPNPDALMLVTLPRLDAATQKSAWFTALQNGGVALKIDPVDRAQLPNWIGQRLALQGQR
AAPGDDGRRALQFIAERVEGNLLAAHQEIQKLGLLYPQGALSFEQVHDAVLNVARYDVFKLNEAMLAGDAARLARMIDGL
KGEGEAIVLVMWAVVEELRTLLRIKRGTTAGKPLATLLRENRVWGPRERLIGPALNRVSEAVLERALAFAAKLDRQVKGL
TAVAPGRRTQDEPPPDPWDGLFQLAMTVAGARGERPPTAGRVRR

Sequences:

>Translated_364_residues
MQLRLDALEPHLAKGLAGLYTVYGDEPLLAQEACDRIRAAARAAGFTERSVHTVERGFDWSVLLGATQAMSLFGERQLIE
LRIPSGKPGKEGADALKTLAATPNPDALMLVTLPRLDAATQKSAWFTALQNGGVALKIDPVDRAQLPNWIGQRLALQGQR
AAPGDDGRRALQFIAERVEGNLLAAHQEIQKLGLLYPQGALSFEQVHDAVLNVARYDVFKLNEAMLAGDAARLARMIDGL
KGEGEAIVLVMWAVVEELRTLLRIKRGTTAGKPLATLLRENRVWGPRERLIGPALNRVSEAVLERALAFAAKLDRQVKGL
TAVAPGRRTQDEPPPDPWDGLFQLAMTVAGARGERPPTAGRVRR
>Mature_364_residues
MQLRLDALEPHLAKGLAGLYTVYGDEPLLAQEACDRIRAAARAAGFTERSVHTVERGFDWSVLLGATQAMSLFGERQLIE
LRIPSGKPGKEGADALKTLAATPNPDALMLVTLPRLDAATQKSAWFTALQNGGVALKIDPVDRAQLPNWIGQRLALQGQR
AAPGDDGRRALQFIAERVEGNLLAAHQEIQKLGLLYPQGALSFEQVHDAVLNVARYDVFKLNEAMLAGDAARLARMIDGL
KGEGEAIVLVMWAVVEELRTLLRIKRGTTAGKPLATLLRENRVWGPRERLIGPALNRVSEAVLERALAFAAKLDRQVKGL
TAVAPGRRTQDEPPPDPWDGLFQLAMTVAGARGERPPTAGRVRR

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The delta subunit seems to interact with the gamma subunit to transfer the b

COG id: COG1466

COG function: function code L; DNA polymerase III, delta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786859, Length=313, Percent_Identity=33.8658146964856, Blast_Score=158, Evalue=6e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR010372
- InterPro:   IPR005790 [H]

Pfam domain/function: PF06144 DNA_pol3_delta [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 39576; Mature: 39576

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLRLDALEPHLAKGLAGLYTVYGDEPLLAQEACDRIRAAARAAGFTERSVHTVERGFDW
CCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCH
SVLLGATQAMSLFGERQLIELRIPSGKPGKEGADALKTLAATPNPDALMLVTLPRLDAAT
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCHH
QKSAWFTALQNGGVALKIDPVDRAQLPNWIGQRLALQGQRAAPGDDGRRALQFIAERVEG
HHHHHHHHHCCCCEEEEECCCCHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCC
NLLAAHQEIQKLGLLYPQGALSFEQVHDAVLNVARYDVFKLNEAMLAGDAARLARMIDGL
CHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCC
KGEGEAIVLVMWAVVEELRTLLRIKRGTTAGKPLATLLRENRVWGPRERLIGPALNRVSE
CCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
AVLERALAFAAKLDRQVKGLTAVAPGRRTQDEPPPDPWDGLFQLAMTVAGARGERPPTAG
HHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
RVRR
CCCC
>Mature Secondary Structure
MQLRLDALEPHLAKGLAGLYTVYGDEPLLAQEACDRIRAAARAAGFTERSVHTVERGFDW
CCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCH
SVLLGATQAMSLFGERQLIELRIPSGKPGKEGADALKTLAATPNPDALMLVTLPRLDAAT
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCHH
QKSAWFTALQNGGVALKIDPVDRAQLPNWIGQRLALQGQRAAPGDDGRRALQFIAERVEG
HHHHHHHHHCCCCEEEEECCCCHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCC
NLLAAHQEIQKLGLLYPQGALSFEQVHDAVLNVARYDVFKLNEAMLAGDAARLARMIDGL
CHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCC
KGEGEAIVLVMWAVVEELRTLLRIKRGTTAGKPLATLLRENRVWGPRERLIGPALNRVSE
CCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
AVLERALAFAAKLDRQVKGLTAVAPGRRTQDEPPPDPWDGLFQLAMTVAGARGERPPTAG
HHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
RVRR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8505303; 1400251; 8905232; 9278503; 3316191; 8505304; 1575709 [H]