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The map label for this gene is dut

Identifier: 78049582

GI number: 78049582

Start: 4623554

End: 4624021

Strand: Reverse

Name: dut

Synonym: XCV4026

Alternate gene names: 78049582

Gene position: 4624021-4623554 (Counterclockwise)

Preceding gene: 78049583

Following gene: 78049581

Centisome position: 89.29

GC content: 68.8

Gene sequence:

>468_bases
ATGAGCCCCCCAACCCAGTCCTTGCAGGTGAAGTTGCTCGACCCGCGCTTCGGCGATCTGTGGCCGCTGCCGGCCTACGC
CACCGAATCCAGCGCCGGCATGGACCTGCGCGCCGCGCTGGAGGCGCCGATGACGCTGCAGCCGGGCGATGCGGCGCTGA
TCCCCAGCGGTATCGCGATCCATCTGGCCGACCCGCAGCTGTGCGCGGTGATCCTGCCGCGCTCGGGGTTGGGCCATCGC
CACGGCATCGTGCTCGGCAACGGCACCGGTTTGATCGATGCCGATTACCAGGGGCCGCTGCTGATCAGCACCTGGAATCG
CGGCCGCGAGGCCTTCACCATCGAGCCGGGCGACCGCATCGCGCAGCTGGTGATCCTGCCGATCGTGCGTATGGGCTTGC
AAGTGGTGGATACTTTCGTCGACAGCGCGCGGGGAGCGGGTGGATTCGGCCACACCGGCGTGCGCTGA

Upstream 100 bases:

>100_bases
CCACGGCCTATTGGCAGGGCGGCGAGCGTGTGTTCCCCAGCAGCAGCAAGACCGAGTTGGCAGACCAACTGCTGGCCCTG
ATCGCGGAGAGATTGCAGGC

Downstream 100 bases:

>100_bases
CAGGGGAAATTACATGAGCAAGGCGAACGAGCGCAGGCAGGGGATGTCCAGCGTGCGTACGAGTGGTCCGGTGTTGGGAG
GCATGTTGCTGCTGCTGGCT

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 155; Mature: 154

Protein sequence:

>155_residues
MSPPTQSLQVKLLDPRFGDLWPLPAYATESSAGMDLRAALEAPMTLQPGDAALIPSGIAIHLADPQLCAVILPRSGLGHR
HGIVLGNGTGLIDADYQGPLLISTWNRGREAFTIEPGDRIAQLVILPIVRMGLQVVDTFVDSARGAGGFGHTGVR

Sequences:

>Translated_155_residues
MSPPTQSLQVKLLDPRFGDLWPLPAYATESSAGMDLRAALEAPMTLQPGDAALIPSGIAIHLADPQLCAVILPRSGLGHR
HGIVLGNGTGLIDADYQGPLLISTWNRGREAFTIEPGDRIAQLVILPIVRMGLQVVDTFVDSARGAGGFGHTGVR
>Mature_154_residues
SPPTQSLQVKLLDPRFGDLWPLPAYATESSAGMDLRAALEAPMTLQPGDAALIPSGIAIHLADPQLCAVILPRSGLGHRH
GIVLGNGTGLIDADYQGPLLISTWNRGREAFTIEPGDRIAQLVILPIVRMGLQVVDTFVDSARGAGGFGHTGVR

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI4503423, Length=151, Percent_Identity=35.0993377483444, Blast_Score=83, Evalue=8e-17,
Organism=Homo sapiens, GI70906444, Length=145, Percent_Identity=35.8620689655172, Blast_Score=83, Evalue=1e-16,
Organism=Homo sapiens, GI70906441, Length=151, Percent_Identity=35.0993377483444, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI1790071, Length=148, Percent_Identity=58.7837837837838, Blast_Score=193, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI71988561, Length=150, Percent_Identity=34.6666666666667, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6319729, Length=84, Percent_Identity=46.4285714285714, Blast_Score=74, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19921126, Length=156, Percent_Identity=29.4871794871795, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24583610, Length=132, Percent_Identity=31.8181818181818, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_XANAC (Q8PFR5)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_644214.1
- ProteinModelPortal:   Q8PFR5
- SMR:   Q8PFR5
- GeneID:   1157984
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC3913
- HOGENOM:   HBG436079
- OMA:   LDLRACI
- ProtClustDB:   PRK00601
- BioCyc:   XAXO190486:XAC3913-MONOMER
- BRENDA:   3.6.1.23
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 16354; Mature: 16223

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: BINDING 87-87

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPPTQSLQVKLLDPRFGDLWPLPAYATESSAGMDLRAALEAPMTLQPGDAALIPSGIAI
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEHHHCCCCEECCCCCEEECCCEEE
HLADPQLCAVILPRSGLGHRHGIVLGNGTGLIDADYQGPLLISTWNRGREAFTIEPGDRI
EECCCCEEEEEECCCCCCCCCCEEEECCCEEEECCCCCCEEEEECCCCCEEEEECCCHHH
AQLVILPIVRMGLQVVDTFVDSARGAGGFGHTGVR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SPPTQSLQVKLLDPRFGDLWPLPAYATESSAGMDLRAALEAPMTLQPGDAALIPSGIAI
CCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEHHHCCCCEECCCCCEEECCCEEE
HLADPQLCAVILPRSGLGHRHGIVLGNGTGLIDADYQGPLLISTWNRGREAFTIEPGDRI
EECCCCEEEEEECCCCCCCCCCEEEECCCEEEECCCCCCEEEEECCCCCEEEEECCCHHH
AQLVILPIVRMGLQVVDTFVDSARGAGGFGHTGVR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217