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The map label for this gene is emrB [H]

Identifier: 78048208

GI number: 78048208

Start: 2987582

End: 2989168

Strand: Reverse

Name: emrB [H]

Synonym: XCV2652

Alternate gene names: 78048208

Gene position: 2989168-2987582 (Counterclockwise)

Preceding gene: 78048209

Following gene: 78048207

Centisome position: 57.72

GC content: 66.16

Gene sequence:

>1587_bases
ATGAGTGCAGCGGCCGCCACCGGCCCGCGTGCCGGCGGCAGCGCCCAGCGCGAGAAGGCCGAGCCGGGCGCCTGGCTGGC
GGTGCTGGCCGGCACCATCGGCTCGTTCATGGCGACGCTGGATATTTCCATCGTCAATGCCGCGCTGCCCACCATCCAGG
GCGAGGTCGGCGCCAGCGGTACAGAAGGCACCTGGATTTCCACCGCCTACCTGGTGGCCGAGATCATCATGATCCCGCTG
ACCGGCTGGTTCGTGCGCACGCTGGGCCTGCGCAATTTCCTGCTGATCTGCGCGGTGATGTTCACCGCGTTCTCGGTGGT
GTGCGGGCTGTCGACGTCGCTTTCGATGATGATCATCGGCCGCGTCGGGCAGGGCTTGGCCGGCGGCGCCCTGATTCCGA
CCGCGCTGACCATCGTCGCCACGCGGTTGCCGCCCAGCCAGCAGACCATGGGGACCGCCTTGTTCGGCATGACCGTGATC
ATGGGCCCGGTGATCGGCCCGCTGCTCGGCGGCTGGCTGACCGAAAACGTCAGCTGGCACTACGCGTTCTTCATCAACGT
GCCGATCTGCGTTGGCCTGGTTGCGCTGCTGCTGCTCGGCCTCAAGCACGAAAAGGGCGACTGGGCAGGCCTGCTCGACG
CCGATTGGCTGGGCATCTACGGGTTGACCGCGGGTCTGGGCGGGCTGACCGTGGTGCTGGAAGAAGGCCAGCGCGAGCGC
TGGTTCGAATCCAGCGAGATCAATACCCTGAGCCTGCTTGCGTTGAGCGGCTTCATCGCCCTGGTGATCAGCCAGTTCCG
GCGGCGCCCTCCGGTGATCCGGCTGTCGCTGCTGCTGCAGCGCAGCTTCGGCGCGGTGTTCATCATGGTCATGGCGGTGG
GCATGATCCTGTTCGGCGTCATGTACATGATTCCGCAGTTCCTGGCGGTGATCTCCGGCTACAACACCGAGCAGGCCGGG
TACGTGCTGCTGCTGTCCGGCCTGCCGACCGTGCTGCTGATGCCGATGATGCCCAAGCTGCTGGAAACGGTGGATGTGCG
CATCCTGGTCATTGCCGGGCTGATCTGCTTTGCCGCTGCCTGCTTCGTCAATCTGTCGCTGACTGCCGACACCGTGGGCA
CGCATTTTGTCGCCGGGCAGCTGCTGCAGGGCTGCGGCCTGGCGTTGGCGATGATGTCGCTCAACCAGGCGGCGATTTCG
TCGGTGCCGCCGGAACTGGCGGGCGATGCCTCGGGCCTGTTCAATGCCGGCCGCAACCTGGGCGGCTCGGTCGGGCTGGC
GCTGATCTCCACCTTTCAGGAGCGCCGCATGACCTTCCACACCGAAACCATCGGCAGCTCGATCACCGCCAACGCCAGCC
GTGCACAGGAGGCGCTGGCCGGCCTGACCGCACAGATGCAGGGCAGCGCCGGCGGCGAGGCGGCAGCCCGCTCGATCGCC
CAGTTCGCCCGCGTCGTACAGCAGCAGGCCCTGGTGATGACCTATAGCGACCTGTTCTGGATCTTTGGCGTGATCGTGGT
CTGCACGATTCCGCTGGCCTTTTTGCTCAAGCCGTTACCCAAGGGGGCGCACCTTGCAATGCACTGA

Upstream 100 bases:

>100_bases
GCATGTCGGTGGAGGTCACCGTGGACACGCGCTCGGCCAAGGACGCCAAGCAGCGTGCCGAAGAAGAATCCGACCGCGTG
CAGGAGCAGGAGAGCGCGCG

Downstream 100 bases:

>100_bases
TTCCATGCGCCTGATCCGCATGCCGCTGGCTGCGGCGTTGGGCACGCTGCTGCTTGGCGGCTGCATGCTCGGCCCCAACT
ACACCAAGCCGCCAGCGGTT

Product: major facilitator superfamily protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MSAAAATGPRAGGSAQREKAEPGAWLAVLAGTIGSFMATLDISIVNAALPTIQGEVGASGTEGTWISTAYLVAEIIMIPL
TGWFVRTLGLRNFLLICAVMFTAFSVVCGLSTSLSMMIIGRVGQGLAGGALIPTALTIVATRLPPSQQTMGTALFGMTVI
MGPVIGPLLGGWLTENVSWHYAFFINVPICVGLVALLLLGLKHEKGDWAGLLDADWLGIYGLTAGLGGLTVVLEEGQRER
WFESSEINTLSLLALSGFIALVISQFRRRPPVIRLSLLLQRSFGAVFIMVMAVGMILFGVMYMIPQFLAVISGYNTEQAG
YVLLLSGLPTVLLMPMMPKLLETVDVRILVIAGLICFAAACFVNLSLTADTVGTHFVAGQLLQGCGLALAMMSLNQAAIS
SVPPELAGDASGLFNAGRNLGGSVGLALISTFQERRMTFHTETIGSSITANASRAQEALAGLTAQMQGSAGGEAAARSIA
QFARVVQQQALVMTYSDLFWIFGVIVVCTIPLAFLLKPLPKGAHLAMH

Sequences:

>Translated_528_residues
MSAAAATGPRAGGSAQREKAEPGAWLAVLAGTIGSFMATLDISIVNAALPTIQGEVGASGTEGTWISTAYLVAEIIMIPL
TGWFVRTLGLRNFLLICAVMFTAFSVVCGLSTSLSMMIIGRVGQGLAGGALIPTALTIVATRLPPSQQTMGTALFGMTVI
MGPVIGPLLGGWLTENVSWHYAFFINVPICVGLVALLLLGLKHEKGDWAGLLDADWLGIYGLTAGLGGLTVVLEEGQRER
WFESSEINTLSLLALSGFIALVISQFRRRPPVIRLSLLLQRSFGAVFIMVMAVGMILFGVMYMIPQFLAVISGYNTEQAG
YVLLLSGLPTVLLMPMMPKLLETVDVRILVIAGLICFAAACFVNLSLTADTVGTHFVAGQLLQGCGLALAMMSLNQAAIS
SVPPELAGDASGLFNAGRNLGGSVGLALISTFQERRMTFHTETIGSSITANASRAQEALAGLTAQMQGSAGGEAAARSIA
QFARVVQQQALVMTYSDLFWIFGVIVVCTIPLAFLLKPLPKGAHLAMH
>Mature_527_residues
SAAAATGPRAGGSAQREKAEPGAWLAVLAGTIGSFMATLDISIVNAALPTIQGEVGASGTEGTWISTAYLVAEIIMIPLT
GWFVRTLGLRNFLLICAVMFTAFSVVCGLSTSLSMMIIGRVGQGLAGGALIPTALTIVATRLPPSQQTMGTALFGMTVIM
GPVIGPLLGGWLTENVSWHYAFFINVPICVGLVALLLLGLKHEKGDWAGLLDADWLGIYGLTAGLGGLTVVLEEGQRERW
FESSEINTLSLLALSGFIALVISQFRRRPPVIRLSLLLQRSFGAVFIMVMAVGMILFGVMYMIPQFLAVISGYNTEQAGY
VLLLSGLPTVLLMPMMPKLLETVDVRILVIAGLICFAAACFVNLSLTADTVGTHFVAGQLLQGCGLALAMMSLNQAAISS
VPPELAGDASGLFNAGRNLGGSVGLALISTFQERRMTFHTETIGSSITANASRAQEALAGLTAQMQGSAGGEAAARSIAQ
FARVVQQQALVMTYSDLFWIFGVIVVCTIPLAFLLKPLPKGAHLAMH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=508, Percent_Identity=32.0866141732283, Blast_Score=258, Evalue=8e-70,
Organism=Escherichia coli, GI1788710, Length=497, Percent_Identity=30.5835010060362, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI1790195, Length=352, Percent_Identity=28.6931818181818, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI87081983, Length=427, Percent_Identity=28.1030444964871, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788392, Length=411, Percent_Identity=25.3041362530414, Blast_Score=91, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6322958, Length=213, Percent_Identity=27.2300469483568, Blast_Score=80, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6321663, Length=149, Percent_Identity=29.5302013422819, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=167, Percent_Identity=26.9461077844311, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55483; Mature: 55352

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAAATGPRAGGSAQREKAEPGAWLAVLAGTIGSFMATLDISIVNAALPTIQGEVGASG
CCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TEGTWISTAYLVAEIIMIPLTGWFVRTLGLRNFLLICAVMFTAFSVVCGLSTSLSMMIIG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RVGQGLAGGALIPTALTIVATRLPPSQQTMGTALFGMTVIMGPVIGPLLGGWLTENVSWH
HHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
YAFFINVPICVGLVALLLLGLKHEKGDWAGLLDADWLGIYGLTAGLGGLTVVLEEGQRER
EEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCHHH
WFESSEINTLSLLALSGFIALVISQFRRRPPVIRLSLLLQRSFGAVFIMVMAVGMILFGV
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MYMIPQFLAVISGYNTEQAGYVLLLSGLPTVLLMPMMPKLLETVDVRILVIAGLICFAAA
HHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CFVNLSLTADTVGTHFVAGQLLQGCGLALAMMSLNQAAISSVPPELAGDASGLFNAGRNL
HHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHCCCC
GGSVGLALISTFQERRMTFHTETIGSSITANASRAQEALAGLTAQMQGSAGGEAAARSIA
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
QFARVVQQQALVMTYSDLFWIFGVIVVCTIPLAFLLKPLPKGAHLAMH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SAAAATGPRAGGSAQREKAEPGAWLAVLAGTIGSFMATLDISIVNAALPTIQGEVGASG
CCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TEGTWISTAYLVAEIIMIPLTGWFVRTLGLRNFLLICAVMFTAFSVVCGLSTSLSMMIIG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RVGQGLAGGALIPTALTIVATRLPPSQQTMGTALFGMTVIMGPVIGPLLGGWLTENVSWH
HHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
YAFFINVPICVGLVALLLLGLKHEKGDWAGLLDADWLGIYGLTAGLGGLTVVLEEGQRER
EEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCHHH
WFESSEINTLSLLALSGFIALVISQFRRRPPVIRLSLLLQRSFGAVFIMVMAVGMILFGV
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MYMIPQFLAVISGYNTEQAGYVLLLSGLPTVLLMPMMPKLLETVDVRILVIAGLICFAAA
HHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CFVNLSLTADTVGTHFVAGQLLQGCGLALAMMSLNQAAISSVPPELAGDASGLFNAGRNL
HHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHCCCC
GGSVGLALISTFQERRMTFHTETIGSSITANASRAQEALAGLTAQMQGSAGGEAAARSIA
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
QFARVVQQQALVMTYSDLFWIFGVIVVCTIPLAFLLKPLPKGAHLAMH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]