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The map label for this gene is pcrA [H]

Identifier: 78045143

GI number: 78045143

Start: 972468

End: 974624

Strand: Direct

Name: pcrA [H]

Synonym: CHY_1099

Alternate gene names: 78045143

Gene position: 972468-974624 (Clockwise)

Preceding gene: 78044353

Following gene: 78043193

Centisome position: 40.49

GC content: 43.16

Gene sequence:

>2157_bases
ATGGAGAAGCTGTTAGCTACTTTAAACCCAGCTCAAAGAGAAGCGGTTACCCATACCCATGGGCCACTTTTAGTTTTGGC
CGGAGCGGGAACGGGAAAAACCAGAGTTTTAACCTTCCGTATCGCTCACTTGCTTTATCGGAAAATGGCCTACCCCCATG
AAATTTTAGCCATAACTTTTACCAATAAAGCGGCCCGCGAAATGAAAGAACGGGTAGAAAGGTTAATCTCGGGAAGTGCC
CGGGAAATCTGGATTTCTACTTTTCATTCTTTTGCGTTAAGAATATTACGGAATGATATTGAAAAACTTGGTTTTAGCCG
GGATTTTACCGTTTATGATGAGGAAGACCAATTGCAGGTAATAAAAGAGTGTTTAAAAGAGCTAAATATTGACGATAAAC
GGTTTGCTCCCAAAGCTATTGCCTACCATATTTCCTCGGCCAAGGACAAGCTTATTTCTCCCCGGCAGTATAGAGACGAT
GCCGATGACTTATTTAAAGAAAAAGTGGCGATAATTTATAATATGTACCAGGAAAAGCTTAATAAAAATAATGCCCTTGA
CTTTGATGATTTACTTTATTATGCGGTAAGACTATTTGAAAATTATCCTCGCGTTCTTGAATACTACCAGCGGCGATTTA
AATATATTCTGGTGGATGAGTATCAGGATACAAGCCATGCCCAGTACCGGCTGGTAAATCTTTTAGCGCAAAAACACCGC
AATCTCTGTGTGGTGGGAGATCCCGACCAGAGCATTTACTCTTGGCGGGGAGCGGATATTACCAATATCTTAAATTTTGA
ACGGGACTATCCGGAAGCAAAAGTGGTGATCTTAAAAGAAAATTACCGCTCCACCCAGAAAATCCTCGATGCGGCTAATG
CGGTAATTGCCAAAAACCGGATGCGCAAGGAAAAAGAGCTTTTTTCTACCAAAGAATCCGGTCATCCGGTTTACTTTTAT
CCTGCAGTGGATGAAAAGGACGAAGCGGCGTTTGTGGCTTTAACCATCGAATCTTTGGTGAAAAGCGGCGAGTATAATTT
CAATGACATAGCTCTTTTGTATCGAACCCATGCTTTATCCCGGAACTTTGAAGAAGCGTTTATGCAAAGAGGCATTCCGT
ACGTGATTTTTGGTAGCAGACGCTTTTATGATCGCAAGGAGATAAAAGACATTATTGCTTATTTACGGATAGTAGCCAAT
CCTTATGATAAAGTGGCTTTAAAAAGAATCATTAATGAACCCAAGCGAGGAATCGGTGAGGCGAGTTTTGCCAAAGTTGA
AGCTTATGCCGACGAAACTGGACTACCCATAGGGTTATTGCTTTTAAGGGATGAAGCTTTGGCTTTGGTTTCCGGAAAAA
CCAGGAAAGCTTTAAAGGAATTTGGCGAAATGCTCCTCTGGTTTCGGGAATTAAAGGACAGGGTAAGTGTTACCAAACTT
ACCGAAGAAATTTTGGAGCGTTCCGGGTATCTACCGGCGTTAAAAGCGGAAGATACCATGGAAGCAAGGGCGAGAATTGA
AAACTTAAACGAGTTTTTGACTGTAACCCAGGCTTTTGAACGGGAGAGCGAAGATAAGTCTTTAGAGGCGTTTTTAGGTA
CCATATCCCTGTTAACCGATGCGGATACTGAAGAAGGTGGCGAAAACCGGGTGCGGATGATGACCCTCCATGCCGCCAAA
GGTCTGGAGTTTCCGGTGGTTTTTCTGGTGGGTTTAGAGGAAGGGATATTTCCTCACAGCCGGGCCATTTTCAGTATTGA
CCCCCAGGAATTAGAAGAAGAAAGAAGGCTTTGCTATGTAGGCATTACCCGGGCGCAAAAGAGGCTTTATCTTACCCGGG
CCTGGCGGCGGACGGTTTACGGACGGACCGACTTTAACGAGCCTTCCCGCTTTTTAGATGAAATACCTGAAGAATTAATT
GAAAGTTTATTAGAAACAGTTTCCGGAGGTAATCCAGTTGACAAAACTCTTTCTCCGGGGGGAGAAAAGCAAAAGGGGGC
TTTTTCTCCGGGAGATAGGGTAAGCCATAAAAAGTTTGGAGTGGGAACGGTGCTGGAAGTTAAACCCAAAGAGGGAGATG
TAATTATAAAAGTGAGATTTGAAGGTGGTGAAGTAAAAGAACTTCTGGGAAGTTTTGCTCCTCTGGTAAAAGTCTAA

Upstream 100 bases:

>100_bases
AATCAAGTACAAACTTTTAGTTTTTGCTGAAGGGATTACCGGCAGTGGCGTTGAATGGTATAATGGGATGGGAAAAATAT
CGAGTAGATGGAGGATATCG

Downstream 100 bases:

>100_bases
GGAGGTGGATGAATGGATAAGGAAGCTGTCAAAAAGCGTATCGAAGAACTTAGGGCCCTTCTTCATTATCACAACTACCG
GTATTATGTTTTGGACCAGC

Product: ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 718; Mature: 718

Protein sequence:

>718_residues
MEKLLATLNPAQREAVTHTHGPLLVLAGAGTGKTRVLTFRIAHLLYRKMAYPHEILAITFTNKAAREMKERVERLISGSA
REIWISTFHSFALRILRNDIEKLGFSRDFTVYDEEDQLQVIKECLKELNIDDKRFAPKAIAYHISSAKDKLISPRQYRDD
ADDLFKEKVAIIYNMYQEKLNKNNALDFDDLLYYAVRLFENYPRVLEYYQRRFKYILVDEYQDTSHAQYRLVNLLAQKHR
NLCVVGDPDQSIYSWRGADITNILNFERDYPEAKVVILKENYRSTQKILDAANAVIAKNRMRKEKELFSTKESGHPVYFY
PAVDEKDEAAFVALTIESLVKSGEYNFNDIALLYRTHALSRNFEEAFMQRGIPYVIFGSRRFYDRKEIKDIIAYLRIVAN
PYDKVALKRIINEPKRGIGEASFAKVEAYADETGLPIGLLLLRDEALALVSGKTRKALKEFGEMLLWFRELKDRVSVTKL
TEEILERSGYLPALKAEDTMEARARIENLNEFLTVTQAFERESEDKSLEAFLGTISLLTDADTEEGGENRVRMMTLHAAK
GLEFPVVFLVGLEEGIFPHSRAIFSIDPQELEEERRLCYVGITRAQKRLYLTRAWRRTVYGRTDFNEPSRFLDEIPEELI
ESLLETVSGGNPVDKTLSPGGEKQKGAFSPGDRVSHKKFGVGTVLEVKPKEGDVIIKVRFEGGEVKELLGSFAPLVKV

Sequences:

>Translated_718_residues
MEKLLATLNPAQREAVTHTHGPLLVLAGAGTGKTRVLTFRIAHLLYRKMAYPHEILAITFTNKAAREMKERVERLISGSA
REIWISTFHSFALRILRNDIEKLGFSRDFTVYDEEDQLQVIKECLKELNIDDKRFAPKAIAYHISSAKDKLISPRQYRDD
ADDLFKEKVAIIYNMYQEKLNKNNALDFDDLLYYAVRLFENYPRVLEYYQRRFKYILVDEYQDTSHAQYRLVNLLAQKHR
NLCVVGDPDQSIYSWRGADITNILNFERDYPEAKVVILKENYRSTQKILDAANAVIAKNRMRKEKELFSTKESGHPVYFY
PAVDEKDEAAFVALTIESLVKSGEYNFNDIALLYRTHALSRNFEEAFMQRGIPYVIFGSRRFYDRKEIKDIIAYLRIVAN
PYDKVALKRIINEPKRGIGEASFAKVEAYADETGLPIGLLLLRDEALALVSGKTRKALKEFGEMLLWFRELKDRVSVTKL
TEEILERSGYLPALKAEDTMEARARIENLNEFLTVTQAFERESEDKSLEAFLGTISLLTDADTEEGGENRVRMMTLHAAK
GLEFPVVFLVGLEEGIFPHSRAIFSIDPQELEEERRLCYVGITRAQKRLYLTRAWRRTVYGRTDFNEPSRFLDEIPEELI
ESLLETVSGGNPVDKTLSPGGEKQKGAFSPGDRVSHKKFGVGTVLEVKPKEGDVIIKVRFEGGEVKELLGSFAPLVKV
>Mature_718_residues
MEKLLATLNPAQREAVTHTHGPLLVLAGAGTGKTRVLTFRIAHLLYRKMAYPHEILAITFTNKAAREMKERVERLISGSA
REIWISTFHSFALRILRNDIEKLGFSRDFTVYDEEDQLQVIKECLKELNIDDKRFAPKAIAYHISSAKDKLISPRQYRDD
ADDLFKEKVAIIYNMYQEKLNKNNALDFDDLLYYAVRLFENYPRVLEYYQRRFKYILVDEYQDTSHAQYRLVNLLAQKHR
NLCVVGDPDQSIYSWRGADITNILNFERDYPEAKVVILKENYRSTQKILDAANAVIAKNRMRKEKELFSTKESGHPVYFY
PAVDEKDEAAFVALTIESLVKSGEYNFNDIALLYRTHALSRNFEEAFMQRGIPYVIFGSRRFYDRKEIKDIIAYLRIVAN
PYDKVALKRIINEPKRGIGEASFAKVEAYADETGLPIGLLLLRDEALALVSGKTRKALKEFGEMLLWFRELKDRVSVTKL
TEEILERSGYLPALKAEDTMEARARIENLNEFLTVTQAFERESEDKSLEAFLGTISLLTDADTEEGGENRVRMMTLHAAK
GLEFPVVFLVGLEEGIFPHSRAIFSIDPQELEEERRLCYVGITRAQKRLYLTRAWRRTVYGRTDFNEPSRFLDEIPEELI
ESLLETVSGGNPVDKTLSPGGEKQKGAFSPGDRVSHKKFGVGTVLEVKPKEGDVIIKVRFEGGEVKELLGSFAPLVKV

Specific function: DNA helicase used for plasmid rolling-circle replication and also involved in UV repair [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=724, Percent_Identity=39.5027624309392, Blast_Score=509, Evalue=1e-145,
Organism=Escherichia coli, GI48994965, Length=646, Percent_Identity=40.7120743034056, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI1787196, Length=343, Percent_Identity=29.1545189504373, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6322369, Length=737, Percent_Identity=30.2578018995929, Blast_Score=257, Evalue=4e-69,
Organism=Saccharomyces cerevisiae, GI6324477, Length=673, Percent_Identity=23.9227340267459, Blast_Score=117, Evalue=5e-27,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 82461; Mature: 82461

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLLATLNPAQREAVTHTHGPLLVLAGAGTGKTRVLTFRIAHLLYRKMAYPHEILAITF
CCCHHHHCCHHHHHHHHCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHCCCCEEEEEEE
TNKAAREMKERVERLISGSAREIWISTFHSFALRILRNDIEKLGFSRDFTVYDEEDQLQV
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHH
IKECLKELNIDDKRFAPKAIAYHISSAKDKLISPRQYRDDADDLFKEKVAIIYNMYQEKL
HHHHHHHCCCCCHHCCCHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHH
NKNNALDFDDLLYYAVRLFENYPRVLEYYQRRFKYILVDEYQDTSHAQYRLVNLLAQKHR
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHCC
NLCVVGDPDQSIYSWRGADITNILNFERDYPEAKVVILKENYRSTQKILDAANAVIAKNR
CEEEECCCCCHHHEECCCCHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHH
MRKEKELFSTKESGHPVYFYPAVDEKDEAAFVALTIESLVKSGEYNFNDIALLYRTHALS
HHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEEHHHHHHCCCCCCCHHHHHHHHHHHH
RNFEEAFMQRGIPYVIFGSRRFYDRKEIKDIIAYLRIVANPYDKVALKRIINEPKRGIGE
CCHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHCCCC
ASFAKVEAYADETGLPIGLLLLRDEALALVSGKTRKALKEFGEMLLWFRELKDRVSVTKL
HHHHHHHHHHCCCCCCEEEEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TEEILERSGYLPALKAEDTMEARARIENLNEFLTVTQAFERESEDKSLEAFLGTISLLTD
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
ADTEEGGENRVRMMTLHAAKGLEFPVVFLVGLEEGIFPHSRAIFSIDPQELEEERRLCYV
CCCCCCCCCCEEEEEEEHHCCCCCCEEEEEECCCCCCCCCCEEEECCHHHHHHHCCEEEE
GITRAQKRLYLTRAWRRTVYGRTDFNEPSRFLDEIPEELIESLLETVSGGNPVDKTLSPG
EHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GEKQKGAFSPGDRVSHKKFGVGTVLEVKPKEGDVIIKVRFEGGEVKELLGSFAPLVKV
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEKLLATLNPAQREAVTHTHGPLLVLAGAGTGKTRVLTFRIAHLLYRKMAYPHEILAITF
CCCHHHHCCHHHHHHHHCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHCCCCEEEEEEE
TNKAAREMKERVERLISGSAREIWISTFHSFALRILRNDIEKLGFSRDFTVYDEEDQLQV
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHH
IKECLKELNIDDKRFAPKAIAYHISSAKDKLISPRQYRDDADDLFKEKVAIIYNMYQEKL
HHHHHHHCCCCCHHCCCHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHH
NKNNALDFDDLLYYAVRLFENYPRVLEYYQRRFKYILVDEYQDTSHAQYRLVNLLAQKHR
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHCC
NLCVVGDPDQSIYSWRGADITNILNFERDYPEAKVVILKENYRSTQKILDAANAVIAKNR
CEEEECCCCCHHHEECCCCHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHH
MRKEKELFSTKESGHPVYFYPAVDEKDEAAFVALTIESLVKSGEYNFNDIALLYRTHALS
HHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEEHHHHHHCCCCCCCHHHHHHHHHHHH
RNFEEAFMQRGIPYVIFGSRRFYDRKEIKDIIAYLRIVANPYDKVALKRIINEPKRGIGE
CCHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHCCCC
ASFAKVEAYADETGLPIGLLLLRDEALALVSGKTRKALKEFGEMLLWFRELKDRVSVTKL
HHHHHHHHHHCCCCCCEEEEEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TEEILERSGYLPALKAEDTMEARARIENLNEFLTVTQAFERESEDKSLEAFLGTISLLTD
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
ADTEEGGENRVRMMTLHAAKGLEFPVVFLVGLEEGIFPHSRAIFSIDPQELEEERRLCYV
CCCCCCCCCCEEEEEEEHHCCCCCCEEEEEECCCCCCCCCCEEEECCHHHHHHHCCEEEE
GITRAQKRLYLTRAWRRTVYGRTDFNEPSRFLDEIPEELIESLLETVSGGNPVDKTLSPG
EHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GEKQKGAFSPGDRVSHKKFGVGTVLEVKPKEGDVIIKVRFEGGEVKELLGSFAPLVKV
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9701819; 9384377 [H]