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The map label for this gene is ligA

Identifier: 78043193

GI number: 78043193

Start: 974637

End: 976631

Strand: Direct

Name: ligA

Synonym: CHY_1100

Alternate gene names: 78043193

Gene position: 974637-976631 (Clockwise)

Preceding gene: 78045143

Following gene: 78045230

Centisome position: 40.58

GC content: 47.67

Gene sequence:

>1995_bases
ATGGATAAGGAAGCTGTCAAAAAGCGTATCGAAGAACTTAGGGCCCTTCTTCATTATCACAACTACCGGTATTATGTTTT
GGACCAGCCGGAAATAAGCGATGCCGAATATGACCGGATGCTCCGGGAGTTAATTTCTTTGGAGCAGCAGTATCCCGAGT
TTATCACTCCCGATTCTCCATCCCAGCGGGTGGGAGGGGAAGTTGCCAAAGAATTTCGGGAAGTGGCTCATTTAAAACCG
ATGTACAGTCTTGATAACGCCTTTGGACCGGAGGATTTAAAGGAATTTGACCGGAGGGTCCGGTCACTGTTACCCGGGCA
GGAGGTAGAGTATGAGGTAGAGTTAAAAATCGACGGTCTGGCTATATCTTTGGTGTATGAAAACGGAGTTTTGGTGCGAG
GAGCCACCCGGGGTAACGGTACCACGGGGGAAGATATTACGGCTAACGTTAAAACCATTAAAGCTATACCTCTAAAATTA
CGAAACCCCATACCCCTTTTAGAAGTTCGGGGCGAAGCGTACATGCCCAAGGAATCTTTTGCCCGCTTAAATGAGCAGCG
GGAAGAGCGGGGGGAGCCCTTATTTGCCAATCCCCGGAATGCAGCAGCAGGGTCGTTACGGCAGCTTGACCCCAAAGTTA
CCGCTGAACGGGACTTAAGTGCTTTTATGTATGCTATTGGGGAAGTTCAGGGATACGAGCCCAAAACCCAGGCGGAATTA
ATGGAGTGGCTTTCGGAGCTTGGGTTTAAAGTAAATCCTTACCGGGAAGTATTTAACAATATTGATGACGTTATTAATTA
CTGCCAGAGCTGGCACGAAAAGAGGTTTTCCCTGCCCTATGTTATTGATGGTTTGGTAATTAAAGTAAATAGTCTTGCCC
AGCAGGAAGCTTTGGGGTTTACCGCAAAGTCTCCCCGGTGGGCGATTGCTTATAAGTTTCCAGCGGAAATTGCCGAGACC
CGGTTAAAAGATATTATCGTACGGGTTGGCCGTACCGGGGTTTTAACACCGACGGCTATTTTTGAACCGGTAAGCCTGGC
TGGCACTACCGTGACGCGAGCATCGCTCCATAACGAAGATTACATCCGGGAAAAGGATATTCGGATTGGCGATATCATTA
GGGTGCAAAAAGCTGGCGAAATAATTCCGGAAGTAGTGGAAGTAGTGAAGGAAAAGAGGACCGGTGCTGAGAAGGAGTTT
GTCATGCCTGATACTTGTCCGGTCTGCCAGGGGAAAGCGGTAAGACTTCCCGGGGAAGCAGCCTGGCGGTGTACCAATGC
TTCCTGTCCTGCTCAGTTAAAAGAAGGCATCGTGCATTTTGCGTCAAGAGGAGCTATGAATATTGAAGGCCTGGGACCGG
CGGTGGCCGAGCTCTTATTGGAAGCTGGCTTAATTCATAATTATGCCGACCTTTACTACTTATCTGCCGAAGAGGTTGCC
AGGCTTCCGCGGATGGGGAAAAAATCGGCCGAAAACCTGATTAATGCTATTGAAAAAAGTAAGCAAAACAGTTTAGAACG
GCTTATTTACGGCCTTGGAATTCGGCTTGTAGGGGAAAAAGCTGCGCGGGATTTGGCAGTTCATTTTAAGGAGCTTGACA
AGTTAATTGCCGCCGGGGAAGAGGAGATCATGGCGATTCCTTCTGTGGGGCCCAAAATGGCGGCTTCAATTAAGGCGTTT
TTTGCCCAAAAGGAAAATTTAGAACTTATCGAAAAGTTAAAAGCAGCAGGAGTAAACACTAAGTACCTGGCCGAGGTGCG
GGATAACCGCTTAGAAGGATTAACTTTTGTCTTAACCGGAACCCTTTCATCCTTCACCCGCAAAGAAGCGGAGCAACTCA
TTTTAAGTCTCGGCGGAAAAGTAAGTTCTTCCGTTTCGAAAAAAACCAGTTATGTAGTGGTTGGCGAAGACCCGGGCAGT
AAATTAACCAAAGCCAAAGAGCTGGGTATTCCAATTTTAACCGAAGAGGAGTTTCGCCAGATGGTGATGAGCTAA

Upstream 100 bases:

>100_bases
AGAGGGAGATGTAATTATAAAAGTGAGATTTGAAGGTGGTGAAGTAAAAGAACTTCTGGGAAGTTTTGCTCCTCTGGTAA
AAGTCTAAGGAGGTGGATGA

Downstream 100 bases:

>100_bases
GATTTTCACTATTTTCTTGCCAATGTTTGTTAAATTTTTGTATAATGGCAAAAGGATATTTTTTAAAGGGGGAACTGTTA
TGACAATTTCCTTAAAAGAT

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 664; Mature: 664

Protein sequence:

>664_residues
MDKEAVKKRIEELRALLHYHNYRYYVLDQPEISDAEYDRMLRELISLEQQYPEFITPDSPSQRVGGEVAKEFREVAHLKP
MYSLDNAFGPEDLKEFDRRVRSLLPGQEVEYEVELKIDGLAISLVYENGVLVRGATRGNGTTGEDITANVKTIKAIPLKL
RNPIPLLEVRGEAYMPKESFARLNEQREERGEPLFANPRNAAAGSLRQLDPKVTAERDLSAFMYAIGEVQGYEPKTQAEL
MEWLSELGFKVNPYREVFNNIDDVINYCQSWHEKRFSLPYVIDGLVIKVNSLAQQEALGFTAKSPRWAIAYKFPAEIAET
RLKDIIVRVGRTGVLTPTAIFEPVSLAGTTVTRASLHNEDYIREKDIRIGDIIRVQKAGEIIPEVVEVVKEKRTGAEKEF
VMPDTCPVCQGKAVRLPGEAAWRCTNASCPAQLKEGIVHFASRGAMNIEGLGPAVAELLLEAGLIHNYADLYYLSAEEVA
RLPRMGKKSAENLINAIEKSKQNSLERLIYGLGIRLVGEKAARDLAVHFKELDKLIAAGEEEIMAIPSVGPKMAASIKAF
FAQKENLELIEKLKAAGVNTKYLAEVRDNRLEGLTFVLTGTLSSFTRKEAEQLILSLGGKVSSSVSKKTSYVVVGEDPGS
KLTKAKELGIPILTEEEFRQMVMS

Sequences:

>Translated_664_residues
MDKEAVKKRIEELRALLHYHNYRYYVLDQPEISDAEYDRMLRELISLEQQYPEFITPDSPSQRVGGEVAKEFREVAHLKP
MYSLDNAFGPEDLKEFDRRVRSLLPGQEVEYEVELKIDGLAISLVYENGVLVRGATRGNGTTGEDITANVKTIKAIPLKL
RNPIPLLEVRGEAYMPKESFARLNEQREERGEPLFANPRNAAAGSLRQLDPKVTAERDLSAFMYAIGEVQGYEPKTQAEL
MEWLSELGFKVNPYREVFNNIDDVINYCQSWHEKRFSLPYVIDGLVIKVNSLAQQEALGFTAKSPRWAIAYKFPAEIAET
RLKDIIVRVGRTGVLTPTAIFEPVSLAGTTVTRASLHNEDYIREKDIRIGDIIRVQKAGEIIPEVVEVVKEKRTGAEKEF
VMPDTCPVCQGKAVRLPGEAAWRCTNASCPAQLKEGIVHFASRGAMNIEGLGPAVAELLLEAGLIHNYADLYYLSAEEVA
RLPRMGKKSAENLINAIEKSKQNSLERLIYGLGIRLVGEKAARDLAVHFKELDKLIAAGEEEIMAIPSVGPKMAASIKAF
FAQKENLELIEKLKAAGVNTKYLAEVRDNRLEGLTFVLTGTLSSFTRKEAEQLILSLGGKVSSSVSKKTSYVVVGEDPGS
KLTKAKELGIPILTEEEFRQMVMS
>Mature_664_residues
MDKEAVKKRIEELRALLHYHNYRYYVLDQPEISDAEYDRMLRELISLEQQYPEFITPDSPSQRVGGEVAKEFREVAHLKP
MYSLDNAFGPEDLKEFDRRVRSLLPGQEVEYEVELKIDGLAISLVYENGVLVRGATRGNGTTGEDITANVKTIKAIPLKL
RNPIPLLEVRGEAYMPKESFARLNEQREERGEPLFANPRNAAAGSLRQLDPKVTAERDLSAFMYAIGEVQGYEPKTQAEL
MEWLSELGFKVNPYREVFNNIDDVINYCQSWHEKRFSLPYVIDGLVIKVNSLAQQEALGFTAKSPRWAIAYKFPAEIAET
RLKDIIVRVGRTGVLTPTAIFEPVSLAGTTVTRASLHNEDYIREKDIRIGDIIRVQKAGEIIPEVVEVVKEKRTGAEKEF
VMPDTCPVCQGKAVRLPGEAAWRCTNASCPAQLKEGIVHFASRGAMNIEGLGPAVAELLLEAGLIHNYADLYYLSAEEVA
RLPRMGKKSAENLINAIEKSKQNSLERLIYGLGIRLVGEKAARDLAVHFKELDKLIAAGEEEIMAIPSVGPKMAASIKAF
FAQKENLELIEKLKAAGVNTKYLAEVRDNRLEGLTFVLTGTLSSFTRKEAEQLILSLGGKVSSSVSKKTSYVVVGEDPGS
KLTKAKELGIPILTEEEFRQMVMS

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=76, Percent_Identity=50, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1788750, Length=670, Percent_Identity=47.3134328358209, Blast_Score=602, Evalue=1e-173,
Organism=Escherichia coli, GI87082305, Length=485, Percent_Identity=25.7731958762887, Blast_Score=127, Evalue=2e-30,
Organism=Drosophila melanogaster, GI62472391, Length=73, Percent_Identity=52.0547945205479, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17737765, Length=73, Percent_Identity=52.0547945205479, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_CARHZ (Q3AD38)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_359946.1
- ProteinModelPortal:   Q3AD38
- STRING:   Q3AD38
- GeneID:   3728712
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_1100
- NMPDR:   fig|246194.3.peg.1147
- TIGR:   CHY_1100
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- BioCyc:   CHYD246194:CHY_1100-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 74178; Mature: 74178

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 116-116 BINDING 114-114 BINDING 137-137 BINDING 172-172 BINDING 288-288 BINDING 312-312

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKEAVKKRIEELRALLHYHNYRYYVLDQPEISDAEYDRMLRELISLEQQYPEFITPDSP
CCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
SQRVGGEVAKEFREVAHLKPMYSLDNAFGPEDLKEFDRRVRSLLPGQEVEYEVELKIDGL
HHHHCHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCE
AISLVYENGVLVRGATRGNGTTGEDITANVKTIKAIPLKLRNPIPLLEVRGEAYMPKESF
EEEEEEECCEEEEECCCCCCCCCCCCCCCCCEEEEECHHCCCCCCEEEECCCCCCCHHHH
ARLNEQREERGEPLFANPRNAAAGSLRQLDPKVTAERDLSAFMYAIGEVQGYEPKTQAEL
HHHHHHHHHCCCCCCCCCCCCHHCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHH
MEWLSELGFKVNPYREVFNNIDDVINYCQSWHEKRFSLPYVIDGLVIKVNSLAQQEALGF
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEHHHHHHHHHCCC
TAKSPRWAIAYKFPAEIAETRLKDIIVRVGRTGVLTPTAIFEPVSLAGTTVTRASLHNED
CCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHCCCHHHHHHHCCCH
YIREKDIRIGDIIRVQKAGEIIPEVVEVVKEKRTGAEKEFVMPDTCPVCQGKAVRLPGEA
HHHHCCCCHHHHEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCHH
AWRCTNASCPAQLKEGIVHFASRGAMNIEGLGPAVAELLLEAGLIHNYADLYYLSAEEVA
HEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCHHHHEEECHHHHH
RLPRMGKKSAENLINAIEKSKQNSLERLIYGLGIRLVGEKAARDLAVHFKELDKLIAAGE
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEECHHHHHHHHHHHHHHHHHHHCCC
EEIMAIPSVGPKMAASIKAFFAQKENLELIEKLKAAGVNTKYLAEVRDNRLEGLTFVLTG
HHEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEH
TLSSFTRKEAEQLILSLGGKVSSSVSKKTSYVVVGEDPGSKLTKAKELGIPILTEEEFRQ
HHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCCHHHHHH
MVMS
HHCC
>Mature Secondary Structure
MDKEAVKKRIEELRALLHYHNYRYYVLDQPEISDAEYDRMLRELISLEQQYPEFITPDSP
CCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
SQRVGGEVAKEFREVAHLKPMYSLDNAFGPEDLKEFDRRVRSLLPGQEVEYEVELKIDGL
HHHHCHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCE
AISLVYENGVLVRGATRGNGTTGEDITANVKTIKAIPLKLRNPIPLLEVRGEAYMPKESF
EEEEEEECCEEEEECCCCCCCCCCCCCCCCCEEEEECHHCCCCCCEEEECCCCCCCHHHH
ARLNEQREERGEPLFANPRNAAAGSLRQLDPKVTAERDLSAFMYAIGEVQGYEPKTQAEL
HHHHHHHHHCCCCCCCCCCCCHHCCHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHH
MEWLSELGFKVNPYREVFNNIDDVINYCQSWHEKRFSLPYVIDGLVIKVNSLAQQEALGF
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEHHHHHHHHHCCC
TAKSPRWAIAYKFPAEIAETRLKDIIVRVGRTGVLTPTAIFEPVSLAGTTVTRASLHNED
CCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHCCCHHHHHHHCCCH
YIREKDIRIGDIIRVQKAGEIIPEVVEVVKEKRTGAEKEFVMPDTCPVCQGKAVRLPGEA
HHHHCCCCHHHHEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCHH
AWRCTNASCPAQLKEGIVHFASRGAMNIEGLGPAVAELLLEAGLIHNYADLYYLSAEEVA
HEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCHHHHEEECHHHHH
RLPRMGKKSAENLINAIEKSKQNSLERLIYGLGIRLVGEKAARDLAVHFKELDKLIAAGE
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEECHHHHHHHHHHHHHHHHHHHCCC
EEIMAIPSVGPKMAASIKAFFAQKENLELIEKLKAAGVNTKYLAEVRDNRLEGLTFVLTG
HHEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEH
TLSSFTRKEAEQLILSLGGKVSSSVSKKTSYVVVGEDPGSKLTKAKELGIPILTEEEFRQ
HHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCCHHHHHH
MVMS
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA