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The map label for this gene is ntrB [H]

Identifier: 78045086

GI number: 78045086

Start: 997163

End: 998518

Strand: Direct

Name: ntrB [H]

Synonym: CHY_1124

Alternate gene names: 78045086

Gene position: 997163-998518 (Clockwise)

Preceding gene: 78043377

Following gene: 78044540

Centisome position: 41.52

GC content: 36.65

Gene sequence:

>1356_bases
ATGAAGCAAAAAATTATAAAAAAAATATTTTTAGTAACTTTGATCGTTGCTGCCATTCTTTTACCCCATGAAGCCACCAC
TTTGGCACGGTTAACCGACCGTTTTATCATCGATAGTGTTTGGGAAGGACAGAGCTCAAAAGTATTTCAAGCGGTGTTAA
TAGGTACGGGTTATTATTTAATGCGGGATATAGGAGCTTTAATTTTCATTTTTTATTTTTTTGAAACGGTAAATAAGCTA
AAACCAATCTTAAAAACCTTTTTATCTTTCTTACTTTTAATTTCCTTTTACGGTTATTTATACTATAAATTTCAAATAGG
CGATTTTGTTTTTGGTATTTTAATTGGATGCGTTCTGTTTTTTTTATTTTCCGGTTTAAAAACCCCGGAAAATATTTTTT
GGAGCAAAAGCTTTTTAGTCCTGCAAGTTCTTCTGGCTTTTTCCTGGTTGAAATACTCCCCGTTTATAAATAAATTTTTC
TATAGCTTTGGCACTCCGGCGGAAGAGGTAAGCTTGGTTGCCCAGTTTTACGGAGCCGATACGGCGTTAAATTGGATAAC
GGTGATTATGTTTTTTATAATTATGATGACGGCTTTAATTTCTACGTTTTTAATATCCATCTACTCCACCCAAATGGAAA
TCATATCTCGCCAGAAAGAAAATGAACTGGAAGTAGAAAGGTACCGGCTCCAGGTCCAAGAAGCCCGGGTTTTTCAGGAA
CTTCACAGTTTAGTTCATGACTTAAAAACACCCTTAATGACGATTCAGGGGTTAAGTTCATTAATTGGCTTAATGGTAGA
TTCTCCAAAATTACAAGAATATGTCCAAAAAATTGAACAGGCAGTGGAAAATGTCAATAAAATGATTTCGGAAATCCTCT
ACGATGATGTTCGGAAGACGATTACCGTTGAAGAATTAATTAATTACGTGCGTGCCCATGTTTTTCCCAAGTATACCGGG
CAGGGTATCTTTTTTGAGCTTTTGGACCAAAAGGAAAAATTATTGATAAACCACATTCGGGTTGCCCGGTCCCTGATTAA
CGTTATCGAAAATGCCTTTGCCGCCACTGCCAATAAACCCGATGGAAGGGTTACCATAAAAACATATAAAGAGGGGAATT
ACATCTGCTTTGAAATTGCCGATAATGGGGTCGGAATACCTCCGGAAATACAAGAGGAAATTTGGACCTGGGGATTTAGT
TTAAAGGAAAAAAAGTCGGGACTGGGATTGCCCTTTGTTAAAAGAGTGGTGGAAAACCATAACGGTTATATTCAGCTTCA
CTCGGAAGTAAATAAAGGAACTACGGTAAAAATATTTTTACCTTTGGGGGGATATGGTGAGAGTACTCATAATTGA

Upstream 100 bases:

>100_bases
GATGGGCTTGTTAATGGATACAACGGATATTACACCAGGGTATTACGCTTTCGCGATTTGAAAGTACCATTAAATAGTAT
TTTAAGGTAACGGTGGGAAA

Downstream 100 bases:

>100_bases
TGACGATGAAGGCATTCGCTACACTGTGGGGGAAATTATTGCCTTTATGGGCTGGGAAGCGATTAAAGCGGAAAACGGAG
CAATAGGCATTGAAAAATTT

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MKQKIIKKIFLVTLIVAAILLPHEATTLARLTDRFIIDSVWEGQSSKVFQAVLIGTGYYLMRDIGALIFIFYFFETVNKL
KPILKTFLSFLLLISFYGYLYYKFQIGDFVFGILIGCVLFFLFSGLKTPENIFWSKSFLVLQVLLAFSWLKYSPFINKFF
YSFGTPAEEVSLVAQFYGADTALNWITVIMFFIIMMTALISTFLISIYSTQMEIISRQKENELEVERYRLQVQEARVFQE
LHSLVHDLKTPLMTIQGLSSLIGLMVDSPKLQEYVQKIEQAVENVNKMISEILYDDVRKTITVEELINYVRAHVFPKYTG
QGIFFELLDQKEKLLINHIRVARSLINVIENAFAATANKPDGRVTIKTYKEGNYICFEIADNGVGIPPEIQEEIWTWGFS
LKEKKSGLGLPFVKRVVENHNGYIQLHSEVNKGTTVKIFLPLGGYGESTHN

Sequences:

>Translated_451_residues
MKQKIIKKIFLVTLIVAAILLPHEATTLARLTDRFIIDSVWEGQSSKVFQAVLIGTGYYLMRDIGALIFIFYFFETVNKL
KPILKTFLSFLLLISFYGYLYYKFQIGDFVFGILIGCVLFFLFSGLKTPENIFWSKSFLVLQVLLAFSWLKYSPFINKFF
YSFGTPAEEVSLVAQFYGADTALNWITVIMFFIIMMTALISTFLISIYSTQMEIISRQKENELEVERYRLQVQEARVFQE
LHSLVHDLKTPLMTIQGLSSLIGLMVDSPKLQEYVQKIEQAVENVNKMISEILYDDVRKTITVEELINYVRAHVFPKYTG
QGIFFELLDQKEKLLINHIRVARSLINVIENAFAATANKPDGRVTIKTYKEGNYICFEIADNGVGIPPEIQEEIWTWGFS
LKEKKSGLGLPFVKRVVENHNGYIQLHSEVNKGTTVKIFLPLGGYGESTHN
>Mature_451_residues
MKQKIIKKIFLVTLIVAAILLPHEATTLARLTDRFIIDSVWEGQSSKVFQAVLIGTGYYLMRDIGALIFIFYFFETVNKL
KPILKTFLSFLLLISFYGYLYYKFQIGDFVFGILIGCVLFFLFSGLKTPENIFWSKSFLVLQVLLAFSWLKYSPFINKFF
YSFGTPAEEVSLVAQFYGADTALNWITVIMFFIIMMTALISTFLISIYSTQMEIISRQKENELEVERYRLQVQEARVFQE
LHSLVHDLKTPLMTIQGLSSLIGLMVDSPKLQEYVQKIEQAVENVNKMISEILYDDVRKTITVEELINYVRAHVFPKYTG
QGIFFELLDQKEKLLINHIRVARSLINVIENAFAATANKPDGRVTIKTYKEGNYICFEIADNGVGIPPEIQEEIWTWGFS
LKEKKSGLGLPFVKRVVENHNGYIQLHSEVNKGTTVKIFLPLGGYGESTHN

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=317, Percent_Identity=25.5520504731861, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790436, Length=208, Percent_Identity=25.4807692307692, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1789808, Length=205, Percent_Identity=25.8536585365854, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1786912, Length=263, Percent_Identity=24.3346007604563, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1790300, Length=245, Percent_Identity=22.8571428571429, Blast_Score=62, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51819; Mature: 51819

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCEEEEECCCCCCCEEEEEEEECCCCCCCCC
>Mature Secondary Structure
MKQKIIKKIFLVTLIVAAILLPHEATTLARLTDRFIIDSVWEGQSSKVFQAVLIGTGYYL
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHH
MRDIGALIFIFYFFETVNKLKPILKTFLSFLLLISFYGYLYYKFQIGDFVFGILIGCVLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHH
FLFSGLKTPENIFWSKSFLVLQVLLAFSWLKYSPFINKFFYSFGTPAEEVSLVAQFYGAD
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCH
TALNWITVIMFFIIMMTALISTFLISIYSTQMEIISRQKENELEVERYRLQVQEARVFQE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LHSLVHDLKTPLMTIQGLSSLIGLMVDSPKLQEYVQKIEQAVENVNKMISEILYDDVRKT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITVEELINYVRAHVFPKYTGQGIFFELLDQKEKLLINHIRVARSLINVIENAFAATANKP
EEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DGRVTIKTYKEGNYICFEIADNGVGIPPEIQEEIWTWGFSLKEKKSGLGLPFVKRVVENH
CCEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHCC
NGYIQLHSEVNKGTTVKIFLPLGGYGESTHN
CCEEEEECCCCCCCEEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]