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The map label for this gene is fliA [H]

Identifier: 78044674

GI number: 78044674

Start: 894543

End: 895307

Strand: Direct

Name: fliA [H]

Synonym: CHY_1013

Alternate gene names: 78044674

Gene position: 894543-895307 (Clockwise)

Preceding gene: 78044241

Following gene: 78044159

Centisome position: 37.25

GC content: 46.41

Gene sequence:

>765_bases
ATGGGAAGTGAGAGCTTGGATGTGGCCGGTTTGTGGAGGGAATATCGGCGAAATAAATCATTGCAACTGAGAAATGAGTT
GCTGGAATATTACCTGCCTTTGGTCCGCAAGGTTTCCGCGGCCATGAGTTTAAAACTTCCCACCCACCTTTCCCGGGAGG
ATATCGAGGGCTACGGCATTATCGGGTTGATTGAAGCGGTGGAAACGTATGATCAGAACCGCGGGGTGAAGTTTGAATCG
TACGCCTCTCTTAAGATTCGGGCGGCAATCATTGACGAGCTGCGGAAGCAAAACTTCCTACCCCGTTCGGTGTGGGATAA
GATAAAAAAAGTAACCACCAGCGAAGAAATGCTGTTAAACGCCCTGGGGGAAGAGGTGCCGGTAGAGAAGGTAGCGGAGC
AGCTGGGAGTAAGCGTCAGCGATGTTTTAAAGATAAAAACCTTAATTGCTTTAAAAAATCACCTTTCCTTAGATGAGATT
ATCGACCCCGAAGGTTCGGTTGACCGTTTTGCAGGGCTGGCGGATGATGAAAAGTATTCGCCGGAAAACAAATTTTTAAA
AAGGGTTGAGATAGAAGAATTGCGAGAAGCGCTAAAGAGGCTTAGTGACCGGGAGCGGTTAATTTTACAGTTATTTTATG
TAGAAGAGCTATCGCTTAAGGAAATTGCCGCAGTGCTGGACATCTCCACATCCCGGGTTTCCCAGATAGCTTCAAAGGCT
TTAGGGAAATTACGGAAATTGTTAAATGAAGGGGTTGGAGTTTAA

Upstream 100 bases:

>100_bases
CATTACGGAATTGAATTTGTAAACATCAGCCGACAGGAGCAGGATACACTTTTTGCGTATTTGTTCCAGGAAATGCAGAA
GTCGCGAAGGATGTTGGCCG

Downstream 100 bases:

>100_bases
TGCTAAGGGGCATTTATATTTCTGCTACTGGCCTGAAAGCAGGACTTAAACAGTTAGATACCGTTGCCAACAACATAGCC
AACATCAATACCGTTGGGTA

Product: RNA polymerase sigma factor for flagellar operon

Products: NA

Alternate protein names: Sigma-28 [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MGSESLDVAGLWREYRRNKSLQLRNELLEYYLPLVRKVSAAMSLKLPTHLSREDIEGYGIIGLIEAVETYDQNRGVKFES
YASLKIRAAIIDELRKQNFLPRSVWDKIKKVTTSEEMLLNALGEEVPVEKVAEQLGVSVSDVLKIKTLIALKNHLSLDEI
IDPEGSVDRFAGLADDEKYSPENKFLKRVEIEELREALKRLSDRERLILQLFYVEELSLKEIAAVLDISTSRVSQIASKA
LGKLRKLLNEGVGV

Sequences:

>Translated_254_residues
MGSESLDVAGLWREYRRNKSLQLRNELLEYYLPLVRKVSAAMSLKLPTHLSREDIEGYGIIGLIEAVETYDQNRGVKFES
YASLKIRAAIIDELRKQNFLPRSVWDKIKKVTTSEEMLLNALGEEVPVEKVAEQLGVSVSDVLKIKTLIALKNHLSLDEI
IDPEGSVDRFAGLADDEKYSPENKFLKRVEIEELREALKRLSDRERLILQLFYVEELSLKEIAAVLDISTSRVSQIASKA
LGKLRKLLNEGVGV
>Mature_253_residues
GSESLDVAGLWREYRRNKSLQLRNELLEYYLPLVRKVSAAMSLKLPTHLSREDIEGYGIIGLIEAVETYDQNRGVKFESY
ASLKIRAAIIDELRKQNFLPRSVWDKIKKVTTSEEMLLNALGEEVPVEKVAEQLGVSVSDVLKIKTLIALKNHLSLDEII
DPEGSVDRFAGLADDEKYSPENKFLKRVEIEELREALKRLSDRERLILQLFYVEELSLKEIAAVLDISTSRVSQIASKAL
GKLRKLLNEGVGV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is required for the transcription of the flagellin and motility genes as well as for wild-

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=226, Percent_Identity=35.8407079646018, Blast_Score=132, Evalue=3e-32,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 28813; Mature: 28682

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSESLDVAGLWREYRRNKSLQLRNELLEYYLPLVRKVSAAMSLKLPTHLSREDIEGYGI
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHH
IGLIEAVETYDQNRGVKFESYASLKIRAAIIDELRKQNFLPRSVWDKIKKVTTSEEMLLN
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHH
ALGEEVPVEKVAEQLGVSVSDVLKIKTLIALKNHLSLDEIIDPEGSVDRFAGLADDEKYS
HHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHCCCCCCCCCC
PENKFLKRVEIEELREALKRLSDRERLILQLFYVEELSLKEIAAVLDISTSRVSQIASKA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGKLRKLLNEGVGV
HHHHHHHHHCCCCC
>Mature Secondary Structure 
GSESLDVAGLWREYRRNKSLQLRNELLEYYLPLVRKVSAAMSLKLPTHLSREDIEGYGI
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHH
IGLIEAVETYDQNRGVKFESYASLKIRAAIIDELRKQNFLPRSVWDKIKKVTTSEEMLLN
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHH
ALGEEVPVEKVAEQLGVSVSDVLKIKTLIALKNHLSLDEIIDPEGSVDRFAGLADDEKYS
HHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHCCCCCCCCCC
PENKFLKRVEIEELREALKRLSDRERLILQLFYVEELSLKEIAAVLDISTSRVSQIASKA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGKLRKLLNEGVGV
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2832368; 9384377; 7602586; 8157612 [H]