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The map label for this gene is purB [H]

Identifier: 78043887

GI number: 78043887

Start: 946183

End: 947475

Strand: Direct

Name: purB [H]

Synonym: CHY_1070

Alternate gene names: 78043887

Gene position: 946183-947475 (Clockwise)

Preceding gene: 78043950

Following gene: 78043284

Centisome position: 39.4

GC content: 45.78

Gene sequence:

>1293_bases
ATGATAGCCCGTTATACTTTACCGGAAATGAAAAAACTCTGGTCGGATGAAACCAAGTACCAAAACTGGCTAAAAGTTGA
AATTGCGGTAGCGGAAGCGATGGGAGAGCTTGGGTTGATACCGCTTGAGGCGGCAAAAGTTATTAAAGAAAAGGCCAACT
TTGATGTAGACCGGATCCTGGAAATTGAAAAAACCGTGAAACACGATGTGATTGCTTTTCTAACCAATGTGGCGGAGTAT
GTGGGAGAGGAAAGTAAGTACATTCATAAAGGTTTAACCTCTTCCGATGTTCTGGATACTGCTCTGGCCCTGCAGTTAAA
AGAAGCGGGAGAGCTATTACTTAGAAAGCTTGAAGGAATTTATCAGCGTTTGCTGGAGCTTGCGAATAAGCACCGCTATA
CGGTGATGATTGGCCGGACCCACGGGGTTCATGCCGAACCGATTACCTTTGGTTTAAAAATGCTTTTGTGGGCGGCGGAA
ATCGAAAGAGACATGGAGCGGCTAAAAACGGCAATTAAAACGGTAAGCGTGGGCAAGATTTCGGGAGCGGTGGGAACCTA
TGCCAATGTGCCTCCGGAAGTGGAAAAACGGGTTTGTGAAAAATTGGGTTTAACTCCCGAGAAAGTTTCCACCCAGATTA
TCCAGAGGGACCGGCATGCCGAGTTTGTCTTAACCCTGGCTTTAATCGCTACCACTTTTGATAAATTTGCCACCGAAATC
CGAACACTACAGCGGACCGAAATCTTGGAGCTGGAGGAGCCGTTTACCGAAGGGCAAAAAGGTTCTTCGGCAATGCCCCA
TAAAAGAAATCCGGTATTATGCGAGCGGATTTCCGGGCTTGCCCGGGTAGTGAGAAGCTATGTGGTGGCGGCGCTGGAAA
ATGTCGTTCTCTGGCAGGAGCGGGATATCAGCCACTCGTCGGCGGAGCGAATGATTTTACCCGATGCAACTTCCACCCTT
TACTACATGATGCACGTTTTTCGCGAAGTTATCGATAACCTAAATATCTATCCGGAACGGATGGAAAGGAATTTAAACCT
TACCGGCGGTTTGATTTTTTCCCAGAGGGTGCTTTTGGCTTTGGTGGAAAAAGGAATATTACGGGAAACCGCTTATAAAA
TTGTGCAGCGGAATGCGATGACCGCCTGGAAAACGGGCAAAAGCTTTAAGGAGCTGTTACTTTCCGATGAAGAGTTAACC
AAACTGTTAACACTCGAAGAGATCGCCGAGTTATTTGATTATAATTACCACTTAAAATACGTGGATGAAATCTTTAAAAG
ATTTGGTTTATAG

Upstream 100 bases:

>100_bases
AAAACTTCAAGCTTATAAAGAAAAGCTGGCCCGGGAAGTGGAAGAAAAGGAGCAAAAACTTTTAGAAAAATTGTTTTAGT
TCATTTTGGAGGTGTTTTTG

Downstream 100 bases:

>100_bases
GGGAGGTATGAAAATGGAGAAAAAGTTTCTTTATGAAGGTAAGGCCAAAATTGTTTATGCCACCGAAAATCCCGATGAAG
TGGTAGTGTACTACAAAGAT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MIARYTLPEMKKLWSDETKYQNWLKVEIAVAEAMGELGLIPLEAAKVIKEKANFDVDRILEIEKTVKHDVIAFLTNVAEY
VGEESKYIHKGLTSSDVLDTALALQLKEAGELLLRKLEGIYQRLLELANKHRYTVMIGRTHGVHAEPITFGLKMLLWAAE
IERDMERLKTAIKTVSVGKISGAVGTYANVPPEVEKRVCEKLGLTPEKVSTQIIQRDRHAEFVLTLALIATTFDKFATEI
RTLQRTEILELEEPFTEGQKGSSAMPHKRNPVLCERISGLARVVRSYVVAALENVVLWQERDISHSSAERMILPDATSTL
YYMMHVFREVIDNLNIYPERMERNLNLTGGLIFSQRVLLALVEKGILRETAYKIVQRNAMTAWKTGKSFKELLLSDEELT
KLLTLEEIAELFDYNYHLKYVDEIFKRFGL

Sequences:

>Translated_430_residues
MIARYTLPEMKKLWSDETKYQNWLKVEIAVAEAMGELGLIPLEAAKVIKEKANFDVDRILEIEKTVKHDVIAFLTNVAEY
VGEESKYIHKGLTSSDVLDTALALQLKEAGELLLRKLEGIYQRLLELANKHRYTVMIGRTHGVHAEPITFGLKMLLWAAE
IERDMERLKTAIKTVSVGKISGAVGTYANVPPEVEKRVCEKLGLTPEKVSTQIIQRDRHAEFVLTLALIATTFDKFATEI
RTLQRTEILELEEPFTEGQKGSSAMPHKRNPVLCERISGLARVVRSYVVAALENVVLWQERDISHSSAERMILPDATSTL
YYMMHVFREVIDNLNIYPERMERNLNLTGGLIFSQRVLLALVEKGILRETAYKIVQRNAMTAWKTGKSFKELLLSDEELT
KLLTLEEIAELFDYNYHLKYVDEIFKRFGL
>Mature_430_residues
MIARYTLPEMKKLWSDETKYQNWLKVEIAVAEAMGELGLIPLEAAKVIKEKANFDVDRILEIEKTVKHDVIAFLTNVAEY
VGEESKYIHKGLTSSDVLDTALALQLKEAGELLLRKLEGIYQRLLELANKHRYTVMIGRTHGVHAEPITFGLKMLLWAAE
IERDMERLKTAIKTVSVGKISGAVGTYANVPPEVEKRVCEKLGLTPEKVSTQIIQRDRHAEFVLTLALIATTFDKFATEI
RTLQRTEILELEEPFTEGQKGSSAMPHKRNPVLCERISGLARVVRSYVVAALENVVLWQERDISHSSAERMILPDATSTL
YYMMHVFREVIDNLNIYPERMERNLNLTGGLIFSQRVLLALVEKGILRETAYKIVQRNAMTAWKTGKSFKELLLSDEELT
KLLTLEEIAELFDYNYHLKYVDEIFKRFGL

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=391, Percent_Identity=29.4117647058824, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI183227688, Length=384, Percent_Identity=29.9479166666667, Blast_Score=159, Evalue=4e-39,
Organism=Escherichia coli, GI1787376, Length=275, Percent_Identity=32, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790398, Length=383, Percent_Identity=25.065274151436, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787896, Length=220, Percent_Identity=30.9090909090909, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17508577, Length=384, Percent_Identity=27.6041666666667, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32564234, Length=317, Percent_Identity=30.5993690851735, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323391, Length=391, Percent_Identity=28.9002557544757, Blast_Score=153, Evalue=6e-38,
Organism=Drosophila melanogaster, GI24647570, Length=387, Percent_Identity=27.906976744186, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI221473854, Length=436, Percent_Identity=24.5412844036697, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI78706858, Length=436, Percent_Identity=24.5412844036697, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI78710009, Length=219, Percent_Identity=29.6803652968037, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49158; Mature: 49158

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIARYTLPEMKKLWSDETKYQNWLKVEIAVAEAMGELGLIPLEAAKVIKEKANFDVDRIL
CCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHH
EIEKTVKHDVIAFLTNVAEYVGEESKYIHKGLTSSDVLDTALALQLKEAGELLLRKLEGI
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YQRLLELANKHRYTVMIGRTHGVHAEPITFGLKMLLWAAEIERDMERLKTAIKTVSVGKI
HHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTYANVPPEVEKRVCEKLGLTPEKVSTQIIQRDRHAEFVLTLALIATTFDKFATEI
CCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTLQRTEILELEEPFTEGQKGSSAMPHKRNPVLCERISGLARVVRSYVVAALENVVLWQE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RDISHSSAERMILPDATSTLYYMMHVFREVIDNLNIYPERMERNLNLTGGLIFSQRVLLA
CCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHHH
LVEKGILRETAYKIVQRNAMTAWKTGKSFKELLLSDEELTKLLTLEEIAELFDYNYHLKY
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHHH
VDEIFKRFGL
HHHHHHHHCC
>Mature Secondary Structure
MIARYTLPEMKKLWSDETKYQNWLKVEIAVAEAMGELGLIPLEAAKVIKEKANFDVDRIL
CCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHH
EIEKTVKHDVIAFLTNVAEYVGEESKYIHKGLTSSDVLDTALALQLKEAGELLLRKLEGI
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YQRLLELANKHRYTVMIGRTHGVHAEPITFGLKMLLWAAEIERDMERLKTAIKTVSVGKI
HHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTYANVPPEVEKRVCEKLGLTPEKVSTQIIQRDRHAEFVLTLALIATTFDKFATEI
CCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RTLQRTEILELEEPFTEGQKGSSAMPHKRNPVLCERISGLARVVRSYVVAALENVVLWQE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RDISHSSAERMILPDATSTLYYMMHVFREVIDNLNIYPERMERNLNLTGGLIFSQRVLLA
CCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHHH
LVEKGILRETAYKIVQRNAMTAWKTGKSFKELLLSDEELTKLLTLEEIAELFDYNYHLKY
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHHH
VDEIFKRFGL
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]