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The map label for this gene is metH [H]

Identifier: 77465348

GI number: 77465348

Start: 411146

End: 413872

Strand: Reverse

Name: metH [H]

Synonym: RSP_3346

Alternate gene names: 77465348

Gene position: 413872-411146 (Counterclockwise)

Preceding gene: 77465349

Following gene: 77465346

Centisome position: 43.89

GC content: 68.57

Gene sequence:

>2727_bases
ATGACCCGTTACCTTCGTCTCTCGGGCCTCGAGCCCTTCGTCCTCACGCCCGACATCCCCTTCGTCAACGTGGGCGAGCG
GACGAACGTCACGGGCTCCGCTCGCTTCCGCAAGCTCATCACCAACCGCGACTATGCGGCGGCGCTCGAGGTCGCGCGCG
ATCAGGTGCAGAACGGCGCGCAGATCCTCGACGTGAACATGGACGAGGGGCTGATCGACTCGAAGGCCGCCATGGTCGAA
TTCCTCAACCTGATCGCGTCCGAGCCCGACATCGCGCGGGTGCCGCTGATGATCGACAGCTCGAAATGGGAGGTGATCGA
GGCCGGACTGAAATGCGTGCAGGGCAAGCCCGTGGTCAATTCGATCAGCCTGAAGGAGGGCGAGGAGTCGTTCCGGCGGC
AGGCGGGCCTCTGCCTCGCCTACGGGGCCGCGGTCGTGGTCATGGCCTTCGACGAGGAGGGGCAGGCCGACAGTTTCGCC
CGCAAGACCACGATCTGCGCCCGGGCCTACCGCATCCTCGTCGAGGAGGTGGGCTTCCCGCCCGAGGACATCATCTTCGA
CCCGAACGTCTTCGCCGTGGCCACGGGCATCGAGGAGCACGACAATTACGGCGTCGACTTCATCGAGGCCGCGCGCTGGA
TCCGGGCGAACCTGCCCCATGCCCATGTCTCGGGCGGCGTGTCGAACCTCTCCTTCAGCTTCCGCGGCAACGAGCCCGTG
CGCGAGGCGATGCATGCGGTCTTCCTCTACCATGCGATCCGGGCCGGGATGGACATGGGGATCGTGAATGCGGGCCAGCT
TGCGGTCTACGACCAGATCGATCCCGACCTGCGCGAGGCCTGCGAGGATGTGGTGCTGAACCGCAAGCCGAAGCAGGGCG
GCACCGCGACCGAGCGGCTCTTGGCTCTGGCCGAGCGGTTCCGGGGCGGCGCGCGCGAGGAGAAGACGCGCGATCTGGCC
TGGCGCGGCTGGCCGGTCGAGAAGCGGCTCGAACATGCGCTGGTCAACGGCATCACCGAATTCATCGAGGCCGACACCGA
AGAGGCCCGGCAGGCGGCCGAGCGGCCGCTCCATGTGATCGAGGGCCCGCTGATGGCGGGGATGAACGTGGTGGGCGACC
TCTTCGGCGCGGGAAAGATGTTCCTGCCGCAGGTGGTGAAGTCGGCCCGGGTGATGAAGCAGGCCGTGGCCGTGCTCCTG
CCCTACATGGAGGAGGAGAAACGTCTCGGCGGCGGCGAGGGCCGCGAGGCGGCGGGCAAGGTGCTGATGGCCACGGTGAA
GGGCGATGTGCATGACATCGGCAAGAACATCGTGGGCGTGGTTCTCGCCTGCAACAATTACGAGATCATCGATCTGGGCG
TGATGGTGCCGCCGGCGAAGATCCTCGAGGTGGCGCGGGCCGAGAAGGTGGATGCGATCGGCCTCTCGGGCCTCATCACG
CCCTCGCTCGACGAGATGGTGACGGTGGCGGCCGAGATGGAACGGGAAGGGTTCGACATCCCGCTGCTGATCGGCGGGGC
CACTACCTCGAAGGTGCATACGGCGGTGAAGATCGCCCCCTCCTATTGCAGGGGCCCCGCGGTCTATGTCACCGATGCAA
GCCGCGCGGTGGGGGTGGTGGGCCAGCTTCTGAGCGCCGAGCGCAAGGGCGCCTATGTCGAGGGGCTGCGCGCCGACTAT
GCCGAGGTGGCCGAGCGCCACGCCCGGTCGGAACGCGCCAAGCAGCGCCTGCCGCTGGCGGCGGCGCGGGCCAATGCGCT
GAAGCTCGACTGGGCCGCGCACCGGCCGGTGCGGCCGAGCTTCACCGGCAGCCGGACCGTCGACGGCTGGGATCTGGCCG
AGATCGCGCGCTACATCGACTGGACGATGTTCTTCCAGACCTGGGAGTTGAAGGGGGTCTATCCGCGCATCCTCGAGGAT
CCGGCGCAGGGCGAGGCCGCCCGCGCGCTCTTTGCCGATGCGCAGGAGATGCTCGCGCGCATCGTGGCGGAACGCTGGTT
CACGCCGCGTGCGGTGGTGGGCTTCTGGCCCGCCAATGCGGTGGGCGACGACATCCGCCTCTATGCCGACGAGACGCGGC
AGGCCGAGCTCGCCACCTTCTTCACCCTCCGCCAGCAGGTCACGAAGCGCGAAGGGCGGCCCAATCTCGCCCTGTCGGAT
TTCGTGGCGCCCGAAGGCGCGGGGCCCGACTGGGTCGGCGGGTTCGTGGTGACGGCCGGCCCCGAGGAGGCCTCCATCGC
CGAGCGGTTCGACCGGGCGAACGACAATTACTCCGCCATCATGGTCAAGGCGCTGGCCGACCGCTTCGCCGAGGCCATGG
CCGAGAGGCTGCACGAGAGGGTGCGCCGCGAGCTCTGGGGCTACGCCCCTGACGAGTCCTTCACGCCCGATGCGCTTCAT
GCCGAGCCCTACCGCGGCATCCGGCCCGCGCCGGGCTACCCGGCCCAGCCCGACCATACCGAGAAGGTCACGCTCTTCCG
CCTGCTCGACGCGACCGCGGCCACGGGGGTGGAACTGACCGAGAGCATGGCCATGTGGCCGGGCTCGTCGGTCTCTGGCC
TCTATATCGGCCATCCCGACGCCTATTACTTCGGCCTCGCGCGGGTCGAGCGCGATCAGGCCGAGGATTATGCCCGTCGC
AAGGGCATGGAACTGGCCGAGGTCGAGCGCTGGCTCGCCCCGGTCATGGCGGGCCGGGTCGACACACCCTCTTCGCGGGC
CGCCTGA

Upstream 100 bases:

>100_bases
AAGGGCTCGTCAATGTCGTGGGCGGCTGCTGCGGCACCACGCCCGACCATGTCCGCGCCATCGCCTGCGCGGTGGCCGAT
CTTCCGCCAAGGACCCTCGT

Downstream 100 bases:

>100_bases
GGGGCGGGCTCACAGGCCCACCCGCCGCGAGGCCACTGTCTCTGTCTCGGGCTCGGCCCGGGGCGGGGCGGCCGGGCGTT
CGGGATGCATGGGCGCGGCC

Product: methionine synthase (B12-dependent)

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 908; Mature: 907

Protein sequence:

>908_residues
MTRYLRLSGLEPFVLTPDIPFVNVGERTNVTGSARFRKLITNRDYAAALEVARDQVQNGAQILDVNMDEGLIDSKAAMVE
FLNLIASEPDIARVPLMIDSSKWEVIEAGLKCVQGKPVVNSISLKEGEESFRRQAGLCLAYGAAVVVMAFDEEGQADSFA
RKTTICARAYRILVEEVGFPPEDIIFDPNVFAVATGIEEHDNYGVDFIEAARWIRANLPHAHVSGGVSNLSFSFRGNEPV
REAMHAVFLYHAIRAGMDMGIVNAGQLAVYDQIDPDLREACEDVVLNRKPKQGGTATERLLALAERFRGGAREEKTRDLA
WRGWPVEKRLEHALVNGITEFIEADTEEARQAAERPLHVIEGPLMAGMNVVGDLFGAGKMFLPQVVKSARVMKQAVAVLL
PYMEEEKRLGGGEGREAAGKVLMATVKGDVHDIGKNIVGVVLACNNYEIIDLGVMVPPAKILEVARAEKVDAIGLSGLIT
PSLDEMVTVAAEMEREGFDIPLLIGGATTSKVHTAVKIAPSYCRGPAVYVTDASRAVGVVGQLLSAERKGAYVEGLRADY
AEVAERHARSERAKQRLPLAAARANALKLDWAAHRPVRPSFTGSRTVDGWDLAEIARYIDWTMFFQTWELKGVYPRILED
PAQGEAARALFADAQEMLARIVAERWFTPRAVVGFWPANAVGDDIRLYADETRQAELATFFTLRQQVTKREGRPNLALSD
FVAPEGAGPDWVGGFVVTAGPEEASIAERFDRANDNYSAIMVKALADRFAEAMAERLHERVRRELWGYAPDESFTPDALH
AEPYRGIRPAPGYPAQPDHTEKVTLFRLLDATAATGVELTESMAMWPGSSVSGLYIGHPDAYYFGLARVERDQAEDYARR
KGMELAEVERWLAPVMAGRVDTPSSRAA

Sequences:

>Translated_908_residues
MTRYLRLSGLEPFVLTPDIPFVNVGERTNVTGSARFRKLITNRDYAAALEVARDQVQNGAQILDVNMDEGLIDSKAAMVE
FLNLIASEPDIARVPLMIDSSKWEVIEAGLKCVQGKPVVNSISLKEGEESFRRQAGLCLAYGAAVVVMAFDEEGQADSFA
RKTTICARAYRILVEEVGFPPEDIIFDPNVFAVATGIEEHDNYGVDFIEAARWIRANLPHAHVSGGVSNLSFSFRGNEPV
REAMHAVFLYHAIRAGMDMGIVNAGQLAVYDQIDPDLREACEDVVLNRKPKQGGTATERLLALAERFRGGAREEKTRDLA
WRGWPVEKRLEHALVNGITEFIEADTEEARQAAERPLHVIEGPLMAGMNVVGDLFGAGKMFLPQVVKSARVMKQAVAVLL
PYMEEEKRLGGGEGREAAGKVLMATVKGDVHDIGKNIVGVVLACNNYEIIDLGVMVPPAKILEVARAEKVDAIGLSGLIT
PSLDEMVTVAAEMEREGFDIPLLIGGATTSKVHTAVKIAPSYCRGPAVYVTDASRAVGVVGQLLSAERKGAYVEGLRADY
AEVAERHARSERAKQRLPLAAARANALKLDWAAHRPVRPSFTGSRTVDGWDLAEIARYIDWTMFFQTWELKGVYPRILED
PAQGEAARALFADAQEMLARIVAERWFTPRAVVGFWPANAVGDDIRLYADETRQAELATFFTLRQQVTKREGRPNLALSD
FVAPEGAGPDWVGGFVVTAGPEEASIAERFDRANDNYSAIMVKALADRFAEAMAERLHERVRRELWGYAPDESFTPDALH
AEPYRGIRPAPGYPAQPDHTEKVTLFRLLDATAATGVELTESMAMWPGSSVSGLYIGHPDAYYFGLARVERDQAEDYARR
KGMELAEVERWLAPVMAGRVDTPSSRAA
>Mature_907_residues
TRYLRLSGLEPFVLTPDIPFVNVGERTNVTGSARFRKLITNRDYAAALEVARDQVQNGAQILDVNMDEGLIDSKAAMVEF
LNLIASEPDIARVPLMIDSSKWEVIEAGLKCVQGKPVVNSISLKEGEESFRRQAGLCLAYGAAVVVMAFDEEGQADSFAR
KTTICARAYRILVEEVGFPPEDIIFDPNVFAVATGIEEHDNYGVDFIEAARWIRANLPHAHVSGGVSNLSFSFRGNEPVR
EAMHAVFLYHAIRAGMDMGIVNAGQLAVYDQIDPDLREACEDVVLNRKPKQGGTATERLLALAERFRGGAREEKTRDLAW
RGWPVEKRLEHALVNGITEFIEADTEEARQAAERPLHVIEGPLMAGMNVVGDLFGAGKMFLPQVVKSARVMKQAVAVLLP
YMEEEKRLGGGEGREAAGKVLMATVKGDVHDIGKNIVGVVLACNNYEIIDLGVMVPPAKILEVARAEKVDAIGLSGLITP
SLDEMVTVAAEMEREGFDIPLLIGGATTSKVHTAVKIAPSYCRGPAVYVTDASRAVGVVGQLLSAERKGAYVEGLRADYA
EVAERHARSERAKQRLPLAAARANALKLDWAAHRPVRPSFTGSRTVDGWDLAEIARYIDWTMFFQTWELKGVYPRILEDP
AQGEAARALFADAQEMLARIVAERWFTPRAVVGFWPANAVGDDIRLYADETRQAELATFFTLRQQVTKREGRPNLALSDF
VAPEGAGPDWVGGFVVTAGPEEASIAERFDRANDNYSAIMVKALADRFAEAMAERLHERVRRELWGYAPDESFTPDALHA
EPYRGIRPAPGYPAQPDHTEKVTLFRLLDATAATGVELTESMAMWPGSSVSGLYIGHPDAYYFGLARVERDQAEDYARRK
GMELAEVERWLAPVMAGRVDTPSSRAA

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=920, Percent_Identity=54.0217391304348, Blast_Score=964, Evalue=0.0,
Organism=Escherichia coli, GI1790450, Length=899, Percent_Identity=61.9577308120133, Blast_Score=1104, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17535405, Length=913, Percent_Identity=53.5596933187295, Blast_Score=937, Evalue=0.0,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 99739; Mature: 99607

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50974 ADOMET_ACTIVATION ; PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRYLRLSGLEPFVLTPDIPFVNVGERTNVTGSARFRKLITNRDYAAALEVARDQVQNGA
CCCEEEECCCCCEEECCCCCEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
QILDVNMDEGLIDSKAAMVEFLNLIASEPDIARVPLMIDSSKWEVIEAGLKCVQGKPVVN
EEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCCC
SISLKEGEESFRRQAGLCLAYGAAVVVMAFDEEGQADSFARKTTICARAYRILVEEVGFP
CCCCCCHHHHHHHHHCHHEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
PEDIIFDPNVFAVATGIEEHDNYGVDFIEAARWIRANLPHAHVSGGVSNLSFSFRGNEPV
CHHEEECCCCEEEEECCHHCCCCCCHHHHHHHHHHHCCCCHHHCCCCCCEEEEECCCCHH
REAMHAVFLYHAIRAGMDMGIVNAGQLAVYDQIDPDLREACEDVVLNRKPKQGGTATERL
HHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
LALAERFRGGAREEKTRDLAWRGWPVEKRLEHALVNGITEFIEADTEEARQAAERPLHVI
HHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHH
EGPLMAGMNVVGDLFGAGKMFLPQVVKSARVMKQAVAVLLPYMEEEKRLGGGEGREAAGK
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCC
VLMATVKGDVHDIGKNIVGVVLACNNYEIIDLGVMVPPAKILEVARAEKVDAIGLSGLIT
EEEEECCCCHHHHHHHHEEEEEEECCEEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCC
PSLDEMVTVAAEMEREGFDIPLLIGGATTSKVHTAVKIAPSYCRGPAVYVTDASRAVGVV
CCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHEEECHHHHCCCEEEEECCHHHHHHH
GQLLSAERKGAYVEGLRADYAEVAERHARSERAKQRLPLAAARANALKLDWAAHRPVRPS
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCCCCCCCC
FTGSRTVDGWDLAEIARYIDWTMFFQTWELKGVYPRILEDPAQGEAARALFADAQEMLAR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHH
IVAERWFTPRAVVGFWPANAVGDDIRLYADETRQAELATFFTLRQQVTKREGRPNLALSD
HHHHHCCCCCCEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHH
FVAPEGAGPDWVGGFVVTAGPEEASIAERFDRANDNYSAIMVKALADRFAEAMAERLHER
CCCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VRRELWGYAPDESFTPDALHAEPYRGIRPAPGYPAQPDHTEKVTLFRLLDATAATGVELT
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHH
ESMAMWPGSSVSGLYIGHPDAYYFGLARVERDQAEDYARRKGMELAEVERWLAPVMAGRV
HHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
DTPSSRAA
CCCCCCCC
>Mature Secondary Structure 
TRYLRLSGLEPFVLTPDIPFVNVGERTNVTGSARFRKLITNRDYAAALEVARDQVQNGA
CCEEEECCCCCEEECCCCCEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
QILDVNMDEGLIDSKAAMVEFLNLIASEPDIARVPLMIDSSKWEVIEAGLKCVQGKPVVN
EEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCCC
SISLKEGEESFRRQAGLCLAYGAAVVVMAFDEEGQADSFARKTTICARAYRILVEEVGFP
CCCCCCHHHHHHHHHCHHEEECCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
PEDIIFDPNVFAVATGIEEHDNYGVDFIEAARWIRANLPHAHVSGGVSNLSFSFRGNEPV
CHHEEECCCCEEEEECCHHCCCCCCHHHHHHHHHHHCCCCHHHCCCCCCEEEEECCCCHH
REAMHAVFLYHAIRAGMDMGIVNAGQLAVYDQIDPDLREACEDVVLNRKPKQGGTATERL
HHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
LALAERFRGGAREEKTRDLAWRGWPVEKRLEHALVNGITEFIEADTEEARQAAERPLHVI
HHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHH
EGPLMAGMNVVGDLFGAGKMFLPQVVKSARVMKQAVAVLLPYMEEEKRLGGGEGREAAGK
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCC
VLMATVKGDVHDIGKNIVGVVLACNNYEIIDLGVMVPPAKILEVARAEKVDAIGLSGLIT
EEEEECCCCHHHHHHHHEEEEEEECCEEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCC
PSLDEMVTVAAEMEREGFDIPLLIGGATTSKVHTAVKIAPSYCRGPAVYVTDASRAVGVV
CCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHEEECHHHHCCCEEEEECCHHHHHHH
GQLLSAERKGAYVEGLRADYAEVAERHARSERAKQRLPLAAARANALKLDWAAHRPVRPS
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCCCCCCCC
FTGSRTVDGWDLAEIARYIDWTMFFQTWELKGVYPRILEDPAQGEAARALFADAQEMLAR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHH
IVAERWFTPRAVVGFWPANAVGDDIRLYADETRQAELATFFTLRQQVTKREGRPNLALSD
HHHHHCCCCCCEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHH
FVAPEGAGPDWVGGFVVTAGPEEASIAERFDRANDNYSAIMVKALADRFAEAMAERLHER
CCCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VRRELWGYAPDESFTPDALHAEPYRGIRPAPGYPAQPDHTEKVTLFRLLDATAATGVELT
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHH
ESMAMWPGSSVSGLYIGHPDAYYFGLARVERDQAEDYARRKGMELAEVERWLAPVMAGRV
HHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
DTPSSRAA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]