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The map label for this gene is metH [H]

Identifier: 77465349

GI number: 77465349

Start: 413869

End: 414948

Strand: Reverse

Name: metH [H]

Synonym: RSP_3347

Alternate gene names: 77465349

Gene position: 414948-413869 (Counterclockwise)

Preceding gene: 77465363

Following gene: 77465348

Centisome position: 44.0

GC content: 69.63

Gene sequence:

>1080_bases
ATGAAGCCCTTCCCGCTATGGTCTCCGCTTCTCCTCCTGATCCGACCGGACTCCATGCTGACCTTTTCCCTTCCGCCCTC
CCGCGCCGTTCCGGCTCTCGAGGCCCTCGTGCGCCAACGCATCCTGATCCTCGACGGGGCGATGGGCACCCAGATCCAGG
CGCTCGGGCTGAGCGAGGAGGATTATGCCGGCTGCGGCTGCCGCCACCACGCCGAGCGGCCGCAGAAGGGCAACAACGAC
CTCCTGATCCTGACCCAGCCGCAGGCCATCGAGGAGATCCATTTCCGCTATGCCATGGCGGGTGCCGACATTGTCGAGAC
CAACACCTTCTCGGCCACCACCATCGCGCAGGCCGATTACGGGATGGAGAGCGCGGTCGAGGATCTGAACCGCGAGGGGG
CGCGGATCGTGCGGCGCGCGCTCGATCGGGCGACGGCGCTCGACGGGCGGCCGCGCTTCGTGGCGGGCGCGGTGGGGCCC
ACCAACCGCACCGCCTCGATCAGCCCCGACGTCAACGATCCGGGCTACCGCGCTGTGAGCTTCGACGATCTGCGCATGGC
CTACGGCACGCAGGTGCGGGCGCTGATCGCGGGCGGCGCGGATCTCATCCTGATCGAGACGATCTTCGACACGCTGAATG
CGAAGGCCGCGATCTTCGCCTGTTTCGAGGCCTTCGCCGAGGCCGGCCGCCGGCTGCCGCTGATGATCTCGGGCACGATC
ACCGACGCGTCGGGCCGCACGCTCTCGGGCCAGACGCCGACCGCCTTCTGGCATTCGGTGCGCCATGCGCGGCCCTTCAC
GGTGGGGCTGAACTGTGCGCTCGGAGCCGCGGCCATGCGGCCCCATCTGGCCGAGCTTGCGGGTGTGGCCGATGCCGCGA
TCTGCGCCTATCCGAACGCGGGCCTGCCCAACGCCTTCGGCCAGTATGACGAGGGCCCGGACGAGACGGCGGCGCAGGTC
GCCCGCTTCGCCCGCGAAGGGCTCGTCAATGTCGTGGGCGGCTGCTGCGGCACCACGCCCGACCATGTCCGCGCCATCGC
CTGCGCGGTGGCCGATCTTCCGCCAAGGACCCTCGTATGA

Upstream 100 bases:

>100_bases
TGCACCTTCTCATGGAAGGCGCAGAGGTCGGGTCGCGATGTGGGCGATGGAAAATCATGAAACACCTTCATGATGATAGT
GCGGGATATGAGAGTGATCG

Downstream 100 bases:

>100_bases
CCCGTTACCTTCGTCTCTCGGGCCTCGAGCCCTTCGTCCTCACGCCCGACATCCCCTTCGTCAACGTGGGCGAGCGGACG
AACGTCACGGGCTCCGCTCG

Product: methionine synthase (B12-dependent)

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MKPFPLWSPLLLLIRPDSMLTFSLPPSRAVPALEALVRQRILILDGAMGTQIQALGLSEEDYAGCGCRHHAERPQKGNND
LLILTQPQAIEEIHFRYAMAGADIVETNTFSATTIAQADYGMESAVEDLNREGARIVRRALDRATALDGRPRFVAGAVGP
TNRTASISPDVNDPGYRAVSFDDLRMAYGTQVRALIAGGADLILIETIFDTLNAKAAIFACFEAFAEAGRRLPLMISGTI
TDASGRTLSGQTPTAFWHSVRHARPFTVGLNCALGAAAMRPHLAELAGVADAAICAYPNAGLPNAFGQYDEGPDETAAQV
ARFAREGLVNVVGGCCGTTPDHVRAIACAVADLPPRTLV

Sequences:

>Translated_359_residues
MKPFPLWSPLLLLIRPDSMLTFSLPPSRAVPALEALVRQRILILDGAMGTQIQALGLSEEDYAGCGCRHHAERPQKGNND
LLILTQPQAIEEIHFRYAMAGADIVETNTFSATTIAQADYGMESAVEDLNREGARIVRRALDRATALDGRPRFVAGAVGP
TNRTASISPDVNDPGYRAVSFDDLRMAYGTQVRALIAGGADLILIETIFDTLNAKAAIFACFEAFAEAGRRLPLMISGTI
TDASGRTLSGQTPTAFWHSVRHARPFTVGLNCALGAAAMRPHLAELAGVADAAICAYPNAGLPNAFGQYDEGPDETAAQV
ARFAREGLVNVVGGCCGTTPDHVRAIACAVADLPPRTLV
>Mature_359_residues
MKPFPLWSPLLLLIRPDSMLTFSLPPSRAVPALEALVRQRILILDGAMGTQIQALGLSEEDYAGCGCRHHAERPQKGNND
LLILTQPQAIEEIHFRYAMAGADIVETNTFSATTIAQADYGMESAVEDLNREGARIVRRALDRATALDGRPRFVAGAVGP
TNRTASISPDVNDPGYRAVSFDDLRMAYGTQVRALIAGGADLILIETIFDTLNAKAAIFACFEAFAEAGRRLPLMISGTI
TDASGRTLSGQTPTAFWHSVRHARPFTVGLNCALGAAAMRPHLAELAGVADAAICAYPNAGLPNAFGQYDEGPDETAAQV
ARFAREGLVNVVGGCCGTTPDHVRAIACAVADLPPRTLV

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG0646

COG function: function code E; Methionine synthase I (cobalamin-dependent), methyltransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=324, Percent_Identity=54.0123456790123, Blast_Score=355, Evalue=5e-98,
Organism=Escherichia coli, GI1790450, Length=335, Percent_Identity=55.5223880597015, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1786456, Length=334, Percent_Identity=27.5449101796407, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17535405, Length=325, Percent_Identity=53.8461538461538, Blast_Score=351, Evalue=4e-97,
Organism=Drosophila melanogaster, GI24585077, Length=279, Percent_Identity=24.3727598566308, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 38210; Mature: 38210

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPFPLWSPLLLLIRPDSMLTFSLPPSRAVPALEALVRQRILILDGAMGTQIQALGLSEE
CCCCCCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHEEEEECCCCCEEEEECCCCC
DYAGCGCRHHAERPQKGNNDLLILTQPQAIEEIHFRYAMAGADIVETNTFSATTIAQADY
CCCCCCCHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCEEECCCCCEEEEEHHHC
GMESAVEDLNREGARIVRRALDRATALDGRPRFVAGAVGPTNRTASISPDVNDPGYRAVS
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEECCCCCCCCCCEEEE
FDDLRMAYGTQVRALIAGGADLILIETIFDTLNAKAAIFACFEAFAEAGRRLPLMISGTI
HHHHHHHHCCCEEEEEECCCCEEEHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEEE
TDASGRTLSGQTPTAFWHSVRHARPFTVGLNCALGAAAMRPHLAELAGVADAAICAYPNA
ECCCCCEECCCCCHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHEEECCCC
GLPNAFGQYDEGPDETAAQVARFAREGLVNVVGGCCGTTPDHVRAIACAVADLPPRTLV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKPFPLWSPLLLLIRPDSMLTFSLPPSRAVPALEALVRQRILILDGAMGTQIQALGLSEE
CCCCCCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHEEEEECCCCCEEEEECCCCC
DYAGCGCRHHAERPQKGNNDLLILTQPQAIEEIHFRYAMAGADIVETNTFSATTIAQADY
CCCCCCCHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCEEECCCCCEEEEEHHHC
GMESAVEDLNREGARIVRRALDRATALDGRPRFVAGAVGPTNRTASISPDVNDPGYRAVS
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEECCCCCCCCCCEEEE
FDDLRMAYGTQVRALIAGGADLILIETIFDTLNAKAAIFACFEAFAEAGRRLPLMISGTI
HHHHHHHHCCCEEEEEECCCCEEEHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEEE
TDASGRTLSGQTPTAFWHSVRHARPFTVGLNCALGAAAMRPHLAELAGVADAAICAYPNA
ECCCCCEECCCCCHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHEEECCCC
GLPNAFGQYDEGPDETAAQVARFAREGLVNVVGGCCGTTPDHVRAIACAVADLPPRTLV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]