Click here to switch to the map view.

The map label for this gene is mnmG

Identifier: 77461958

GI number: 77461958

Start: 6432022

End: 6433920

Strand: Reverse

Name: mnmG

Synonym: Pfl01_5741

Alternate gene names: 77461958

Gene position: 6433920-6432022 (Counterclockwise)

Preceding gene: 77461959

Following gene: 77461957

Centisome position: 99.93

GC content: 59.08

Gene sequence:

>1899_bases
GTGGATTTCCCTTCCCGTTTTGAAGTGATCGTCATCGGCGGCGGTCATGCCGGTACCGAGGCAGCACTGGCCTCAGCACG
TATGGGTGCAAAAACCCTTTTGCTGACGCACAACGTGGAAACCCTCGGTGCCATGAGCTGCAACCCGGCCATCGGCGGCA
TTGGCAAAAGCCATCTGGTCAAGGAAATCGATGCCCTCGGCGGCGCGATGGCCATGGCCACCGATCTGGGCGGTATCCAG
TTTCGCGTATTGAACAGCCGCAAAGGCCCGGCCGTACGTGCAACCCGTGCGCAAGCCGACCGTATTCTTTACAAGGCCGC
TGTCCGCGAAATTCTGGAAAACCAGCCGAACCTGTGGATATTTCAACAAGCCGCTGACGACCTGATCGTCGAGCAGGAAC
AAGTACGCGGTGTTGTCACCCAAATGGGTCTGCGTTTCTTCGCGGATTCCGTGGTGTTGACCACCGGTACGTTCCTGGGC
GGACTTATCCACATCGGTTTGCAGAATTTTTCCGGCGGTCGTGCCGGAGATCCACCGTCGATCGCCCTGGCGCATCGTCT
GCGCGAACTGCCGCTGCGCGTAGGTCGCCTGAAAACCGGTACGCCACCGCGTATCGACGGCAAGTCTGTGGATTTCTCGG
TGATGACCGAGCAACCAGGCGATACGCCGATCCCGGTGATGTCGTTCATGGGCAACAAGGAACAGCATCCACGGCAAGTC
AGTTGCTGGATCACCCACACCAACGCGCGCACCCACGAAATCATTGCCGCGAACCTCGACCGTTCGCCGATGTATTCCGC
TGCAGGCGAAATCGAAGGCATCGGCCCGCGTTATTGCCCGTCGATCGAAGACAAGATCCACCGCTTTGCCGACAAGGAAA
GCCATCAGGTGTTCATCGAGCCGGAAGGCCTGACCACCCATGAGTTGTATCCGAACGGGATATCCACAAGCCTGCCGTTC
GACGTGCAATTGCAGATCGTGCAATCGATCCGCGGCATGGAAAACGCACACATCGTGCGTCCGGGTTATGCGATCGAGTA
CGACTACTTCGATCCGCGCGACCTGAAGTACAGCCTGGAAACCAAAGTCATCGGCGGTCTGTTCTTCGCTGGCCAGATCA
ACGGCACCACCGGTTACGAAGAAGCCGGTGCTCAGGGGTTGCTGGCTGGCGCGAACGCTGCACTGCGTGCGAAAGGCAAA
GAAGCCTGGTGCCCGCGTCGCGATGAAGCGTACATCGGGGTATTGGTCGACGACCTGATCACCCTCGGTACCCAAGAGCC
GTACCGCATGTTCACTTCCCGCGCCGAGTACCGCCTGATCCTGCGTGAGGACAATGCGGACCTGCGTCTGACCGAAAAAG
GTCGCGAACTGGGTCTGGTCGATGATGCGCGCTGGGCGGCGTTCTGCAAGAAACGTGAAAGCATCGAACTCGAAGAGCAG
CGCCTGAAAAGCACCTGGGTTCGCCCGGGCACTCAGCAGGGCGATGCGATTGCCGAGAAGTTCGGCACGCCGCTGACTCA
CGAATACAACTTGCTCAATCTCTTGAGCCGTCCGGAAATCGACTACGCTGGTCTGGTCGAAGTGACCGGACTGGGCGCCG
AAGATCCACAGGTGGCCGAACAGGTCGAGATCAAGACCAAATACGCCGGTTACATCGACCGTCAACAGGACGAAATCGCC
CGTCTGCGCGCCAGCGAAGACACGAAACTGCCTGTGGATATCGATTACACCAACATTTCCGGTCTCTCCAAGGAGATCCA
GAGCAAGCTGGGCGCCACGCGTCCCGAGACGCTTGGCCAGGCTTCGCGGATCCCCGGCGTGACCCCGGCAGCGATTTCGC
TGTTGATGATTCACTTGAAAAAACGCGGCGCGGGCCGTCAGTTGGAGCAAAGCGCTTGA

Upstream 100 bases:

>100_bases
TCTTTAGGAAGGGCGAAGTCCCTATACTTGCCGACCAGGTCCAGAAACCTGATCTCAAACTATTCCTGAATGACCTACTT
AAGCAGGCACGAGGTGCGTG

Downstream 100 bases:

>100_bases
GTTCTAAGGTCACCTCGCAACACGCCGAAGAGTTATCCACAGGAGCCCGCACGCTCGGTGTCAATCTCACTGGAACCCAG
CACGAATTGCTGCTGGGTTA

Product: tRNA uridine 5-carboxymethylaminomethyl modification protein GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 632; Mature: 632

Protein sequence:

>632_residues
MDFPSRFEVIVIGGGHAGTEAALASARMGAKTLLLTHNVETLGAMSCNPAIGGIGKSHLVKEIDALGGAMAMATDLGGIQ
FRVLNSRKGPAVRATRAQADRILYKAAVREILENQPNLWIFQQAADDLIVEQEQVRGVVTQMGLRFFADSVVLTTGTFLG
GLIHIGLQNFSGGRAGDPPSIALAHRLRELPLRVGRLKTGTPPRIDGKSVDFSVMTEQPGDTPIPVMSFMGNKEQHPRQV
SCWITHTNARTHEIIAANLDRSPMYSAAGEIEGIGPRYCPSIEDKIHRFADKESHQVFIEPEGLTTHELYPNGISTSLPF
DVQLQIVQSIRGMENAHIVRPGYAIEYDYFDPRDLKYSLETKVIGGLFFAGQINGTTGYEEAGAQGLLAGANAALRAKGK
EAWCPRRDEAYIGVLVDDLITLGTQEPYRMFTSRAEYRLILREDNADLRLTEKGRELGLVDDARWAAFCKKRESIELEEQ
RLKSTWVRPGTQQGDAIAEKFGTPLTHEYNLLNLLSRPEIDYAGLVEVTGLGAEDPQVAEQVEIKTKYAGYIDRQQDEIA
RLRASEDTKLPVDIDYTNISGLSKEIQSKLGATRPETLGQASRIPGVTPAAISLLMIHLKKRGAGRQLEQSA

Sequences:

>Translated_632_residues
MDFPSRFEVIVIGGGHAGTEAALASARMGAKTLLLTHNVETLGAMSCNPAIGGIGKSHLVKEIDALGGAMAMATDLGGIQ
FRVLNSRKGPAVRATRAQADRILYKAAVREILENQPNLWIFQQAADDLIVEQEQVRGVVTQMGLRFFADSVVLTTGTFLG
GLIHIGLQNFSGGRAGDPPSIALAHRLRELPLRVGRLKTGTPPRIDGKSVDFSVMTEQPGDTPIPVMSFMGNKEQHPRQV
SCWITHTNARTHEIIAANLDRSPMYSAAGEIEGIGPRYCPSIEDKIHRFADKESHQVFIEPEGLTTHELYPNGISTSLPF
DVQLQIVQSIRGMENAHIVRPGYAIEYDYFDPRDLKYSLETKVIGGLFFAGQINGTTGYEEAGAQGLLAGANAALRAKGK
EAWCPRRDEAYIGVLVDDLITLGTQEPYRMFTSRAEYRLILREDNADLRLTEKGRELGLVDDARWAAFCKKRESIELEEQ
RLKSTWVRPGTQQGDAIAEKFGTPLTHEYNLLNLLSRPEIDYAGLVEVTGLGAEDPQVAEQVEIKTKYAGYIDRQQDEIA
RLRASEDTKLPVDIDYTNISGLSKEIQSKLGATRPETLGQASRIPGVTPAAISLLMIHLKKRGAGRQLEQSA
>Mature_632_residues
MDFPSRFEVIVIGGGHAGTEAALASARMGAKTLLLTHNVETLGAMSCNPAIGGIGKSHLVKEIDALGGAMAMATDLGGIQ
FRVLNSRKGPAVRATRAQADRILYKAAVREILENQPNLWIFQQAADDLIVEQEQVRGVVTQMGLRFFADSVVLTTGTFLG
GLIHIGLQNFSGGRAGDPPSIALAHRLRELPLRVGRLKTGTPPRIDGKSVDFSVMTEQPGDTPIPVMSFMGNKEQHPRQV
SCWITHTNARTHEIIAANLDRSPMYSAAGEIEGIGPRYCPSIEDKIHRFADKESHQVFIEPEGLTTHELYPNGISTSLPF
DVQLQIVQSIRGMENAHIVRPGYAIEYDYFDPRDLKYSLETKVIGGLFFAGQINGTTGYEEAGAQGLLAGANAALRAKGK
EAWCPRRDEAYIGVLVDDLITLGTQEPYRMFTSRAEYRLILREDNADLRLTEKGRELGLVDDARWAAFCKKRESIELEEQ
RLKSTWVRPGTQQGDAIAEKFGTPLTHEYNLLNLLSRPEIDYAGLVEVTGLGAEDPQVAEQVEIKTKYAGYIDRQQDEIA
RLRASEDTKLPVDIDYTNISGLSKEIQSKLGATRPETLGQASRIPGVTPAAISLLMIHLKKRGAGRQLEQSA

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=631, Percent_Identity=46.4342313787639, Blast_Score=511, Evalue=1e-144,
Organism=Homo sapiens, GI19882217, Length=656, Percent_Identity=44.6646341463415, Blast_Score=497, Evalue=1e-140,
Organism=Homo sapiens, GI183227703, Length=671, Percent_Identity=43.6661698956781, Blast_Score=492, Evalue=1e-139,
Organism=Escherichia coli, GI2367273, Length=629, Percent_Identity=68.6804451510334, Blast_Score=887, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=631, Percent_Identity=40.4120443740095, Blast_Score=439, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6321202, Length=626, Percent_Identity=43.6102236421725, Blast_Score=493, Evalue=1e-140,
Organism=Drosophila melanogaster, GI24658174, Length=633, Percent_Identity=46.6034755134281, Blast_Score=502, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_PSEPF (Q3K430)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_351465.1
- ProteinModelPortal:   Q3K430
- SMR:   Q3K430
- STRING:   Q3K430
- GeneID:   3714466
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_5741
- eggNOG:   COG0445
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- ProtClustDB:   PRK05192
- BioCyc:   PFLU205922:PFL_5741-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 69307; Mature: 69307

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFPSRFEVIVIGGGHAGTEAALASARMGAKTLLLTHNVETLGAMSCNPAIGGIGKSHLV
CCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCHHHCCCCCCCCCCCCCHHHHH
KEIDALGGAMAMATDLGGIQFRVLNSRKGPAVRATRAQADRILYKAAVREILENQPNLWI
HHHHHHCCHHHHHHCCCCEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCEEE
FQQAADDLIVEQEQVRGVVTQMGLRFFADSVVLTTGTFLGGLIHIGLQNFSGGRAGDPPS
ECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
IALAHRLRELPLRVGRLKTGTPPRIDGKSVDFSVMTEQPGDTPIPVMSFMGNKEQHPRQV
HHHHHHHHHCCHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHCCCCCCCCCEE
SCWITHTNARTHEIIAANLDRSPMYSAAGEIEGIGPRYCPSIEDKIHRFADKESHQVFIE
EEEEEECCCCCEEEEEECCCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEC
PEGLTTHELYPNGISTSLPFDVQLQIVQSIRGMENAHIVRPGYAIEYDYFDPRDLKYSLE
CCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCEEEEECCCCCCCCEEEHH
TKVIGGLFFAGQINGTTGYEEAGAQGLLAGANAALRAKGKEAWCPRRDEAYIGVLVDDLI
HEEEEEEEEEEEECCCCCCHHCCCCEEEECCCCCEEECCCCCCCCCCCCCEEEHHHHHHH
TLGTQEPYRMFTSRAEYRLILREDNADLRLTEKGRELGLVDDARWAAFCKKRESIELEEQ
HCCCCCHHHHHHCCCCEEEEEEECCCCEEEECCCCEECCCCCHHHHHHHHHCCCCCHHHH
RLKSTWVRPGTQQGDAIAEKFGTPLTHEYNLLNLLSRPEIDYAGLVEVTGLGAEDPQVAE
HHHHHHCCCCCCCCHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHH
QVEIKTKYAGYIDRQQDEIARLRASEDTKLPVDIDYTNISGLSKEIQSKLGATRPETLGQ
HHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCHHHCH
ASRIPGVTPAAISLLMIHLKKRGAGRQLEQSA
HHCCCCCCHHHHHHHHHHHHHCCCCCCHHCCC
>Mature Secondary Structure
MDFPSRFEVIVIGGGHAGTEAALASARMGAKTLLLTHNVETLGAMSCNPAIGGIGKSHLV
CCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCHHHCCCCCCCCCCCCCHHHHH
KEIDALGGAMAMATDLGGIQFRVLNSRKGPAVRATRAQADRILYKAAVREILENQPNLWI
HHHHHHCCHHHHHHCCCCEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCEEE
FQQAADDLIVEQEQVRGVVTQMGLRFFADSVVLTTGTFLGGLIHIGLQNFSGGRAGDPPS
ECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
IALAHRLRELPLRVGRLKTGTPPRIDGKSVDFSVMTEQPGDTPIPVMSFMGNKEQHPRQV
HHHHHHHHHCCHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHCCCCCCCCCEE
SCWITHTNARTHEIIAANLDRSPMYSAAGEIEGIGPRYCPSIEDKIHRFADKESHQVFIE
EEEEEECCCCCEEEEEECCCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEC
PEGLTTHELYPNGISTSLPFDVQLQIVQSIRGMENAHIVRPGYAIEYDYFDPRDLKYSLE
CCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCEEEEECCCCCCCCEEEHH
TKVIGGLFFAGQINGTTGYEEAGAQGLLAGANAALRAKGKEAWCPRRDEAYIGVLVDDLI
HEEEEEEEEEEEECCCCCCHHCCCCEEEECCCCCEEECCCCCCCCCCCCCEEEHHHHHHH
TLGTQEPYRMFTSRAEYRLILREDNADLRLTEKGRELGLVDDARWAAFCKKRESIELEEQ
HCCCCCHHHHHHCCCCEEEEEEECCCCEEEECCCCEECCCCCHHHHHHHHHCCCCCHHHH
RLKSTWVRPGTQQGDAIAEKFGTPLTHEYNLLNLLSRPEIDYAGLVEVTGLGAEDPQVAE
HHHHHHCCCCCCCCHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHH
QVEIKTKYAGYIDRQQDEIARLRASEDTKLPVDIDYTNISGLSKEIQSKLGATRPETLGQ
HHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCHHHCH
ASRIPGVTPAAISLLMIHLKKRGAGRQLEQSA
HHCCCCCCHHHHHHHHHHHHHCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA