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The map label for this gene is gidB

Identifier: 77461957

GI number: 77461957

Start: 6431381

End: 6432025

Strand: Reverse

Name: gidB

Synonym: Pfl01_5740

Alternate gene names: 77461957

Gene position: 6432025-6431381 (Counterclockwise)

Preceding gene: 77461958

Following gene: 77461956

Centisome position: 99.9

GC content: 58.91

Gene sequence:

>645_bases
TTGAGTTCTAAGGTCACCTCGCAACACGCCGAAGAGTTATCCACAGGAGCCCGCACGCTCGGTGTCAATCTCACTGGAAC
CCAGCACGAATTGCTGCTGGGTTATCTGGCCCTGTTGATCAAATGGAACCAGGCCTACAACCTGACCGCCGTTCGCGATC
CGGACGAAATGGTGTCCCGTCACTTGCTCGATAGCCTCAGCGTGATGTCGTTCATCGAAAACGGCCGCTGGCTGGATGTC
GGCAGCGGCGGCGGCATGCCGGGGATCCCTTTGGCGATCCTGTATCCGGACTCGCAAGTGACCTGCTTGGACAGCAACGG
CAAGAAAACCCGCTTCCTGACCCAGGTCAAACTCGAACTCAAACTGGATAACCTGCAAGTTATCCACAGTCGCGTCGAAG
CGTTCCAGCCTGCACAGCCGTTCAACGGGATCATTTCCCGGGCGTTCAGCAGCATGGAGAACTTCACCAACTGGACTCGC
CACCTCGGCGACGCCGATACGCGCTGGCTGGCAATGAAGGGCGTCCATCCGGCCGATGAGCTGGTAGCATTGCCGGCAGA
CTTCAAACTCGATAGCGAACACGCCCTGGCCGTACCCGGTTGCCAAGGCCAACGCCATCTGCTGATACTGCGCCGCACGG
CATGA

Upstream 100 bases:

>100_bases
GCCAGGCTTCGCGGATCCCCGGCGTGACCCCGGCAGCGATTTCGCTGTTGATGATTCACTTGAAAAAACGCGGCGCGGGC
CGTCAGTTGGAGCAAAGCGC

Downstream 100 bases:

>100_bases
TTGGGAACACAAGCAAGAATGGCTAAGGTATTCGCGATAGCGAACCAGAAGGGTGGTGTGGGCAAGACCACCACCTGTAT
CAACCTCGCAGCATCCCTGG

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MSSKVTSQHAEELSTGARTLGVNLTGTQHELLLGYLALLIKWNQAYNLTAVRDPDEMVSRHLLDSLSVMSFIENGRWLDV
GSGGGMPGIPLAILYPDSQVTCLDSNGKKTRFLTQVKLELKLDNLQVIHSRVEAFQPAQPFNGIISRAFSSMENFTNWTR
HLGDADTRWLAMKGVHPADELVALPADFKLDSEHALAVPGCQGQRHLLILRRTA

Sequences:

>Translated_214_residues
MSSKVTSQHAEELSTGARTLGVNLTGTQHELLLGYLALLIKWNQAYNLTAVRDPDEMVSRHLLDSLSVMSFIENGRWLDV
GSGGGMPGIPLAILYPDSQVTCLDSNGKKTRFLTQVKLELKLDNLQVIHSRVEAFQPAQPFNGIISRAFSSMENFTNWTR
HLGDADTRWLAMKGVHPADELVALPADFKLDSEHALAVPGCQGQRHLLILRRTA
>Mature_213_residues
SSKVTSQHAEELSTGARTLGVNLTGTQHELLLGYLALLIKWNQAYNLTAVRDPDEMVSRHLLDSLSVMSFIENGRWLDVG
SGGGMPGIPLAILYPDSQVTCLDSNGKKTRFLTQVKLELKLDNLQVIHSRVEAFQPAQPFNGIISRAFSSMENFTNWTRH
LGDADTRWLAMKGVHPADELVALPADFKLDSEHALAVPGCQGQRHLLILRRTA

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=200, Percent_Identity=46, Blast_Score=204, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_PSEPF (Q3K431)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_351464.1
- ProteinModelPortal:   Q3K431
- SMR:   Q3K431
- STRING:   Q3K431
- GeneID:   3714235
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_5740
- eggNOG:   COG0357
- HOGENOM:   HBG686577
- OMA:   HIMDSIV
- ProtClustDB:   PRK00107
- BioCyc:   PFLU205922:PFL_5740-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: =2.1.1.170

Molecular weight: Translated: 23658; Mature: 23527

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKVTSQHAEELSTGARTLGVNLTGTQHELLLGYLALLIKWNQAYNLTAVRDPDEMVSR
CCCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHH
HLLDSLSVMSFIENGRWLDVGSGGGMPGIPLAILYPDSQVTCLDSNGKKTRFLTQVKLEL
HHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCEEEEECCCCCCEEEEEEEEEEE
KLDNLQVIHSRVEAFQPAQPFNGIISRAFSSMENFTNWTRHLGDADTRWLAMKGVHPADE
EECHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHH
LVALPADFKLDSEHALAVPGCQGQRHLLILRRTA
EEECCCCCEECCCCEEECCCCCCCCEEEEEEECC
>Mature Secondary Structure 
SSKVTSQHAEELSTGARTLGVNLTGTQHELLLGYLALLIKWNQAYNLTAVRDPDEMVSR
CCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHH
HLLDSLSVMSFIENGRWLDVGSGGGMPGIPLAILYPDSQVTCLDSNGKKTRFLTQVKLEL
HHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCEEEEECCCCCCEEEEEEEEEEE
KLDNLQVIHSRVEAFQPAQPFNGIISRAFSSMENFTNWTRHLGDADTRWLAMKGVHPADE
EECHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHH
LVALPADFKLDSEHALAVPGCQGQRHLLILRRTA
EEECCCCCEECCCCEEECCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA