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The map label for this gene is trmE [H]

Identifier: 77461959

GI number: 77461959

Start: 6434473

End: 6435843

Strand: Reverse

Name: trmE [H]

Synonym: Pfl01_5742

Alternate gene names: 77461959

Gene position: 6435843-6434473 (Counterclockwise)

Preceding gene: 77461960

Following gene: 77461958

Centisome position: 99.96

GC content: 59.15

Gene sequence:

>1371_bases
ATGAGCGCACCTCGTGAAACCATCGCAGCTGTCGCCACCGCCCAGGGCCGTGGCGGCGTGGGCATCGTTCGAATTTCCGG
GCCGCTGGCCAGTGTTGCGGCCAAAGCCATCAGCGGCCGCGAGCTGAAACCGCGGTTTGCCCACTACGGCCCGTTCTTCA
GTGACAATCAACAGGTGCTGGATGAAGGCCTGGCGCTGTATTTCCCCGGGCCGAACTCGTTCACTGGCGAAGACGTACTG
GAACTGCAGGGCCACGGTGGTCCGATCGTCCTCGACATGTTGCTCAAGCGTTGTCTGGAATTGGGCTGCCGTCTCGCGCG
GCCCGGTGAGTTCAGCGAAAGAGCATTCCTTAATGACAAACTCGACCTGGCCCAGGCCGAGGCCATTGCCGACCTGATCG
AAGCCAGTTCTGCACAGGCCGCGAGAAACGCTCTGCGTTCGTTGCAAGGTGCATTTTCCCAACGTGTGCATAACCTCACC
GAACAGCTGATCGGTTTGCGGATTTATGTCGAGGCTGCCATCGACTTCCCGGAAGAAGAAATCGACTTCCTCGCCGATGG
CCATGTCCTGAGCATGCTCGACAAGGTTCGTAACGAGTTATCCACCGTATTGCGCGAAGCCGGGCAGGGTGCGTTGCTGC
GTGACGGCATGACCGTGGTGATTGCCGGTCGTCCGAATGCCGGCAAATCCAGTCTGCTGAATGCACTGGCCGGCCGTGAA
GCCGCGATCGTGACCGAAATCGCCGGTACCACTCGGGATATCCTGCGTGAACATATCCACATCGATGGCATGCCGCTGCA
TGTCGTGGATACCGCAGGTCTGCGTGATACCGATGATCATGTGGAAAAGATCGGCGTTGAACGAGCGTTGAAAGCCATCG
GCGAGGCTGATCGAGTACTGCTGGTCGTCGATGCCACGGCGCCTGAGGCCGCTGATCCGTTCGCCCTGTGGCCGGAATTC
CTCGAAACCCGACCGGATCCGGCGAAGGTTACATTGATCCGTAACAAGGCCGATCTCACGGGTGAGCCCATTGCCTTGGA
AGTCAGTGACGATGGCCATGTGACGATCAGCCTGAGCGCCAAATCTGCGGGAGAGGGTCTGGATCTGCTGCGTGATCACC
TCAAGGCTTGCATGGGCTACGAACAAACATCGGAAAGCAGCTTCAGCGCACGCCGCCGGCACCTGGAAGCCCTGCGTCAT
GCCAGTGATGCCCTGGAACATGGACGTGCCCAACTGACGTTGGCGGGTGCTGGTGAGCTGCTGGCCGAAGACCTGCGTCA
GGCCCAACAGTCTCTTGGGGAAATCACCGGCGCCTTCAGTTCAGATGATTTGCTGGGACGAATCTTTTCCAGCTTCTGCA
TCGGTAAATAA

Upstream 100 bases:

>100_bases
AAAAAAGCCGAGGCGTAACTTACTCTGTGGATAACACCACTCAAAACGCCCCCTAGTGGGGCGTTTTGCTATCTGCCATT
TTTGTTCTGGATACCGGTTT

Downstream 100 bases:

>100_bases
CCCTTCCGTTGTCCACAGACAGGTCCGTTCGCGACCTGTCTGTTCTCCCTCCCTCGTATTTCCTTCCTCCAGTGCCGGAC
AGATTTCTATTCCTGAGTAG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MSAPRETIAAVATAQGRGGVGIVRISGPLASVAAKAISGRELKPRFAHYGPFFSDNQQVLDEGLALYFPGPNSFTGEDVL
ELQGHGGPIVLDMLLKRCLELGCRLARPGEFSERAFLNDKLDLAQAEAIADLIEASSAQAARNALRSLQGAFSQRVHNLT
EQLIGLRIYVEAAIDFPEEEIDFLADGHVLSMLDKVRNELSTVLREAGQGALLRDGMTVVIAGRPNAGKSSLLNALAGRE
AAIVTEIAGTTRDILREHIHIDGMPLHVVDTAGLRDTDDHVEKIGVERALKAIGEADRVLLVVDATAPEAADPFALWPEF
LETRPDPAKVTLIRNKADLTGEPIALEVSDDGHVTISLSAKSAGEGLDLLRDHLKACMGYEQTSESSFSARRRHLEALRH
ASDALEHGRAQLTLAGAGELLAEDLRQAQQSLGEITGAFSSDDLLGRIFSSFCIGK

Sequences:

>Translated_456_residues
MSAPRETIAAVATAQGRGGVGIVRISGPLASVAAKAISGRELKPRFAHYGPFFSDNQQVLDEGLALYFPGPNSFTGEDVL
ELQGHGGPIVLDMLLKRCLELGCRLARPGEFSERAFLNDKLDLAQAEAIADLIEASSAQAARNALRSLQGAFSQRVHNLT
EQLIGLRIYVEAAIDFPEEEIDFLADGHVLSMLDKVRNELSTVLREAGQGALLRDGMTVVIAGRPNAGKSSLLNALAGRE
AAIVTEIAGTTRDILREHIHIDGMPLHVVDTAGLRDTDDHVEKIGVERALKAIGEADRVLLVVDATAPEAADPFALWPEF
LETRPDPAKVTLIRNKADLTGEPIALEVSDDGHVTISLSAKSAGEGLDLLRDHLKACMGYEQTSESSFSARRRHLEALRH
ASDALEHGRAQLTLAGAGELLAEDLRQAQQSLGEITGAFSSDDLLGRIFSSFCIGK
>Mature_455_residues
SAPRETIAAVATAQGRGGVGIVRISGPLASVAAKAISGRELKPRFAHYGPFFSDNQQVLDEGLALYFPGPNSFTGEDVLE
LQGHGGPIVLDMLLKRCLELGCRLARPGEFSERAFLNDKLDLAQAEAIADLIEASSAQAARNALRSLQGAFSQRVHNLTE
QLIGLRIYVEAAIDFPEEEIDFLADGHVLSMLDKVRNELSTVLREAGQGALLRDGMTVVIAGRPNAGKSSLLNALAGREA
AIVTEIAGTTRDILREHIHIDGMPLHVVDTAGLRDTDDHVEKIGVERALKAIGEADRVLLVVDATAPEAADPFALWPEFL
ETRPDPAKVTLIRNKADLTGEPIALEVSDDGHVTISLSAKSAGEGLDLLRDHLKACMGYEQTSESSFSARRRHLEALRHA
SDALEHGRAQLTLAGAGELLAEDLRQAQQSLGEITGAFSSDDLLGRIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=473, Percent_Identity=36.9978858350951, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI193082993, Length=463, Percent_Identity=36.2850971922246, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI193082988, Length=504, Percent_Identity=34.3253968253968, Blast_Score=211, Evalue=2e-54,
Organism=Escherichia coli, GI2367268, Length=453, Percent_Identity=66.4459161147903, Blast_Score=586, Evalue=1e-168,
Organism=Escherichia coli, GI87082120, Length=92, Percent_Identity=42.3913043478261, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17507259, Length=464, Percent_Identity=31.0344827586207, Blast_Score=180, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6323665, Length=492, Percent_Identity=31.0975609756098, Blast_Score=196, Evalue=7e-51,
Organism=Drosophila melanogaster, GI45550813, Length=478, Percent_Identity=34.3096234309623, Blast_Score=213, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 48865; Mature: 48734

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPRETIAAVATAQGRGGVGIVRISGPLASVAAKAISGRELKPRFAHYGPFFSDNQQVL
CCCCHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCHHHCCCCCCCCHHHH
DEGLALYFPGPNSFTGEDVLELQGHGGPIVLDMLLKRCLELGCRLARPGEFSERAFLNDK
HCCCEEEECCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCC
LDLAQAEAIADLIEASSAQAARNALRSLQGAFSQRVHNLTEQLIGLRIYVEAAIDFPEEE
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCHHH
IDFLADGHVLSMLDKVRNELSTVLREAGQGALLRDGMTVVIAGRPNAGKSSLLNALAGRE
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEECCCCCCHHHHHHHHHCCC
AAIVTEIAGTTRDILREHIHIDGMPLHVVDTAGLRDTDDHVEKIGVERALKAIGEADRVL
HHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEE
LVVDATAPEAADPFALWPEFLETRPDPAKVTLIRNKADLTGEPIALEVSDDGHVTISLSA
EEEECCCCCCCCCHHHCHHHHHCCCCCCEEEEEECCCCCCCCCEEEEECCCCEEEEEEEC
KSAGEGLDLLRDHLKACMGYEQTSESSFSARRRHLEALRHASDALEHGRAQLTLAGAGEL
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHH
LAEDLRQAQQSLGEITGAFSSDDLLGRIFSSFCIGK
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAPRETIAAVATAQGRGGVGIVRISGPLASVAAKAISGRELKPRFAHYGPFFSDNQQVL
CCCHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHCCCCCCCCHHHCCCCCCCCHHHH
DEGLALYFPGPNSFTGEDVLELQGHGGPIVLDMLLKRCLELGCRLARPGEFSERAFLNDK
HCCCEEEECCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCC
LDLAQAEAIADLIEASSAQAARNALRSLQGAFSQRVHNLTEQLIGLRIYVEAAIDFPEEE
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCHHH
IDFLADGHVLSMLDKVRNELSTVLREAGQGALLRDGMTVVIAGRPNAGKSSLLNALAGRE
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEECCCCCCHHHHHHHHHCCC
AAIVTEIAGTTRDILREHIHIDGMPLHVVDTAGLRDTDDHVEKIGVERALKAIGEADRVL
HHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEE
LVVDATAPEAADPFALWPEFLETRPDPAKVTLIRNKADLTGEPIALEVSDDGHVTISLSA
EEEECCCCCCCCCHHHCHHHHHCCCCCCEEEEEECCCCCCCCCEEEEECCCCEEEEEEEC
KSAGEGLDLLRDHLKACMGYEQTSESSFSARRRHLEALRHASDALEHGRAQLTLAGAGEL
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHH
LAEDLRQAQQSLGEITGAFSSDDLLGRIFSSFCIGK
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA