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The map label for this gene is cysL [H]

Identifier: 77459904

GI number: 77459904

Start: 4166643

End: 4167503

Strand: Reverse

Name: cysL [H]

Synonym: Pfl01_3682

Alternate gene names: 77459904

Gene position: 4167503-4166643 (Counterclockwise)

Preceding gene: 77459908

Following gene: 77459902

Centisome position: 64.73

GC content: 65.74

Gene sequence:

>861_bases
ATGAACCTGTTCCAGCTCCGCGCCTTCGACGCCGTGGCCCGGGAGGGCAGCTTCACCCGTGCCGCCGCACGCCTGTTCAT
CAGCCAGCCCGCGGTCACCGGGCACATCAAGGCGCTGGAGGAGCATTACCAGATCACCCTGTTGCGACGCACGGCGCGCC
GGGTCGAGCTGACGGAAGAGGGCACCAAACTGGCAGCGATCACCCGGGCGATGTTCGGTATGGCCGAGGAGGCCCAGGCG
TTGCTCGAGGCCAATCGGCAATTGCTCACCGGACGCCTTGAGGTGGCGGCGGATGGCCCGCACATGGTCATGCCGATGCT
CGCCAGCCTGCGGGCGCGCTATCCGGGGATCACCGTGAATCTGCGGTTGGGCAATGCCCAGGAAACCTTGGCGGCGTTGT
TGTCCGAGCATGCCGATGTGGCGGTCTTGACCGAGGTCGAGCCGCGCAAAGGGCTGCATCTGCAAGCGTTGAGTGAATCG
CGGATCTGCGCGCTGGTGCCGGCCGCGCATCCGTGGGCGACACGTGCAGGCGAGGTCAGGCTCAAGGAGCTGGATCAGGT
GATCATGGTCTTGCGCGAGCCGAGTTCGATTACCCGGCGCACGTTTGATCAGGCCTGTGCCCAGGCATCGGTCAGCCCAC
GGGTGTTGCTGGAACTGGACAGTCGCGAGGCGGTGACGGAAGCGGTGGCGGCTGAACTGGGGGTAGGGGTGGTGTCATCG
GTGGAGGTCAGTCATGACCCCCGTGTCGTCGCGATTCCCATTGCGGGAGAAGGGCTGGTGAACCGGCACATGATCGGCTG
CGTGGAGCGGCGGCGGGAGTTGCGCTTGATTCAGGCGTTTTTCGGGTTGGCCCCGGTCTGA

Upstream 100 bases:

>100_bases
CGATAGCGCTGTGGGATGAAGCTACGGGGCCTACGTTGCAATTCGCCGGATCATCAATCAAATTGTTTGTTGTTATGCCA
GCCATCAGTGAGGGTTATTC

Downstream 100 bases:

>100_bases
ACAAGACTCTCGCGAACCATCTCGAGAAACGTCGCCACCACCCGCCGCGAACTTTGCTCGCGCAGGCACACCAGCGTTTC
GGTCAACCTCCGGGTGCAAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MNLFQLRAFDAVAREGSFTRAAARLFISQPAVTGHIKALEEHYQITLLRRTARRVELTEEGTKLAAITRAMFGMAEEAQA
LLEANRQLLTGRLEVAADGPHMVMPMLASLRARYPGITVNLRLGNAQETLAALLSEHADVAVLTEVEPRKGLHLQALSES
RICALVPAAHPWATRAGEVRLKELDQVIMVLREPSSITRRTFDQACAQASVSPRVLLELDSREAVTEAVAAELGVGVVSS
VEVSHDPRVVAIPIAGEGLVNRHMIGCVERRRELRLIQAFFGLAPV

Sequences:

>Translated_286_residues
MNLFQLRAFDAVAREGSFTRAAARLFISQPAVTGHIKALEEHYQITLLRRTARRVELTEEGTKLAAITRAMFGMAEEAQA
LLEANRQLLTGRLEVAADGPHMVMPMLASLRARYPGITVNLRLGNAQETLAALLSEHADVAVLTEVEPRKGLHLQALSES
RICALVPAAHPWATRAGEVRLKELDQVIMVLREPSSITRRTFDQACAQASVSPRVLLELDSREAVTEAVAAELGVGVVSS
VEVSHDPRVVAIPIAGEGLVNRHMIGCVERRRELRLIQAFFGLAPV
>Mature_286_residues
MNLFQLRAFDAVAREGSFTRAAARLFISQPAVTGHIKALEEHYQITLLRRTARRVELTEEGTKLAAITRAMFGMAEEAQA
LLEANRQLLTGRLEVAADGPHMVMPMLASLRARYPGITVNLRLGNAQETLAALLSEHADVAVLTEVEPRKGLHLQALSES
RICALVPAAHPWATRAGEVRLKELDQVIMVLREPSSITRRTFDQACAQASVSPRVLLELDSREAVTEAVAAELGVGVVSS
VEVSHDPRVVAIPIAGEGLVNRHMIGCVERRRELRLIQAFFGLAPV

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082132, Length=252, Percent_Identity=27.7777777777778, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI157672245, Length=260, Percent_Identity=29.6153846153846, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1788297, Length=242, Percent_Identity=28.9256198347107, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787879, Length=269, Percent_Identity=29.7397769516729, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789173, Length=169, Percent_Identity=35.5029585798817, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1788748, Length=284, Percent_Identity=28.169014084507, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1788481, Length=242, Percent_Identity=26.0330578512397, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI2367136, Length=243, Percent_Identity=28.3950617283951, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786401, Length=141, Percent_Identity=32.6241134751773, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790399, Length=246, Percent_Identity=23.9837398373984, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI145693105, Length=274, Percent_Identity=23.3576642335766, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787530, Length=266, Percent_Identity=23.3082706766917, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787128, Length=250, Percent_Identity=29.2, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31257; Mature: 31257

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLFQLRAFDAVAREGSFTRAAARLFISQPAVTGHIKALEEHYQITLLRRTARRVELTEE
CCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEHHHC
GTKLAAITRAMFGMAEEAQALLEANRQLLTGRLEVAADGPHMVMPMLASLRARYPGITVN
CCHHHHHHHHHHCCHHHHHHHHHCCHHHHHCEEEEECCCCHHHHHHHHHHHHCCCCEEEE
LRLGNAQETLAALLSEHADVAVLTEVEPRKGLHLQALSESRICALVPAAHPWATRAGEVR
EEECCHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCCCEEEEECCCCCCCCCCCCHH
LKELDQVIMVLREPSSITRRTFDQACAQASVSPRVLLELDSREAVTEAVAAELGVGVVSS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCCHHEE
VEVSHDPRVVAIPIAGEGLVNRHMIGCVERRRELRLIQAFFGLAPV
EEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNLFQLRAFDAVAREGSFTRAAARLFISQPAVTGHIKALEEHYQITLLRRTARRVELTEE
CCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEHHHC
GTKLAAITRAMFGMAEEAQALLEANRQLLTGRLEVAADGPHMVMPMLASLRARYPGITVN
CCHHHHHHHHHHCCHHHHHHHHHCCHHHHHCEEEEECCCCHHHHHHHHHHHHCCCCEEEE
LRLGNAQETLAALLSEHADVAVLTEVEPRKGLHLQALSESRICALVPAAHPWATRAGEVR
EEECCHHHHHHHHHHCCCCEEEEEECCCCCCCEEEECCCCCEEEEECCCCCCCCCCCCHH
LKELDQVIMVLREPSSITRRTFDQACAQASVSPRVLLELDSREAVTEAVAAELGVGVVSS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCCHHEE
VEVSHDPRVVAIPIAGEGLVNRHMIGCVERRRELRLIQAFFGLAPV
EEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]