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The map label for this gene is puuB [H]

Identifier: 77459901

GI number: 77459901

Start: 4162695

End: 4164086

Strand: Reverse

Name: puuB [H]

Synonym: Pfl01_3679

Alternate gene names: 77459901

Gene position: 4164086-4162695 (Counterclockwise)

Preceding gene: 77459902

Following gene: 77459899

Centisome position: 64.68

GC content: 64.58

Gene sequence:

>1392_bases
ATGAGACCGTTTTGGCTGGAGCAGGCTTTGCAGCTCGACACGTCCGAACCTTGCCCGCCGCTGCAAGTCGATGTGCGCAC
CGATGTGTGCATCGTCGGCGGCGGTTACACCGGGTTGTGGACGGCGATCATGCTCAAGCAGCAGAACCCTGAACTTGATG
TGTTGCTGATCGAAGCCGACATTTGCGGTGCCGGCGCCAGTGGGCGCAACGGCGGTTGTGCGCTGTCGTGGTCGGCGAAG
TATTTCACCCTGGAGCGGCTGTTCGGTGTCGAGGAAGCGGTGCGACTGGTCAAGGAATCCGAGCGCAGCATCCACGCCAT
CGGCGAGTTCTGCGAGCAGTACGGCGTCGATGCCGATTACCGGCTCGACGGCACGCTTTACACCGCTACCAACCGGGCGC
AATGCGGCTCGACCGATGCGGTAATTGCGGCGCTAGAGCGCAATGGCATCAACTCGTTTACCCAGCGGCCGGTCGCCGAT
GTACAGCGCATGGCCGGTTCGGAAAAACATTTGGAGGGCTGGTTCTCGCCGGCCGCCGCCAGCGTGCAGCCGGGCAAACT
GGTGCGCGGCTTGCGTCGGGTGGCGTTGCAACTGGGCGTGCGGATTCATGAAGGCACGGCGATGACCGGGCTGGAAGAGG
GCCGTCCGGCGCGCCTTCACACGGCGAACGGCCGGGTGACCGCCGACCGCGTGGTGCTGGCGATGAATGCGTGGATGGCC
CGCGCTTTCCCGCAGTTCGAGCGCAGCGTGGCGATTGTCTCCAGCGACATGCTGATCACCGAACCGCGTCCGGATCTGTT
GCAGGAAATCGGTTTGACCAGCGGCGTCACCGTGCTCGACTCGCGGATTTTCGTGCACTACTACCACAACACCCCGGACG
GCCGGATCATGCTCGGCAAGGGCGGCAACACCTTCGCTTACGGCGGGCGGATGCTGCCGGTGTTCGACCAGTCATCGCCC
TATACCGGATTGCTCAAACGCAGCCTCGACGACTTCTTCCCGGCATTCGCGGATGTGAAAGTCGATGCAACGTGGAACGG
CCCTTCGGATCGCTCGGTCACCGGCCTGCCGTTCTTCGGCCAGATGAGTGCCAGCGGCAACGTGTTCTACGGTTTCGGTT
ATTCCGGCAGCGGCGTCGGGCCGTGCCACATGGGCGGGCAGATTCTCGCCTCGCTGGTGCAGGGCCTCGACAACCCGTGG
ACGCGTTCGCCGCTGGTCAACGGGCCGCTGGGTTACTTTCCGCCGGAGCCGATCCGCTACCTCGGCTCGCTGATGGTGCG
CAACGCCATTCGCCGCAAGGAACGCGCCGAGGATCACGGTCGGCGGCCCCGGCACCTTGACGTGCGTCTGGCGAAATTCG
CAGCGGCGGCGGGCAAGGCCGACAAGGCTTGA

Upstream 100 bases:

>100_bases
CCTGACCGTCAAACCCCTGCGTAACTGAAACCCCTTGTCTCGCGGCCTGCTGCGGCACTCGCGGCAGGCTGTGTCGTGGC
CGACTGACTGGAGAATTTTC

Downstream 100 bases:

>100_bases
GCGTCAGCTTGGCGGGGCGGAACGGTTGAGCAGCCAGTCGAGCAGGAGGTCGTCGTCGGGTTCGTTTTCTGTCGGCGGTG
CCTGGCGGAGCCGACAAGCG

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MRPFWLEQALQLDTSEPCPPLQVDVRTDVCIVGGGYTGLWTAIMLKQQNPELDVLLIEADICGAGASGRNGGCALSWSAK
YFTLERLFGVEEAVRLVKESERSIHAIGEFCEQYGVDADYRLDGTLYTATNRAQCGSTDAVIAALERNGINSFTQRPVAD
VQRMAGSEKHLEGWFSPAAASVQPGKLVRGLRRVALQLGVRIHEGTAMTGLEEGRPARLHTANGRVTADRVVLAMNAWMA
RAFPQFERSVAIVSSDMLITEPRPDLLQEIGLTSGVTVLDSRIFVHYYHNTPDGRIMLGKGGNTFAYGGRMLPVFDQSSP
YTGLLKRSLDDFFPAFADVKVDATWNGPSDRSVTGLPFFGQMSASGNVFYGFGYSGSGVGPCHMGGQILASLVQGLDNPW
TRSPLVNGPLGYFPPEPIRYLGSLMVRNAIRRKERAEDHGRRPRHLDVRLAKFAAAAGKADKA

Sequences:

>Translated_463_residues
MRPFWLEQALQLDTSEPCPPLQVDVRTDVCIVGGGYTGLWTAIMLKQQNPELDVLLIEADICGAGASGRNGGCALSWSAK
YFTLERLFGVEEAVRLVKESERSIHAIGEFCEQYGVDADYRLDGTLYTATNRAQCGSTDAVIAALERNGINSFTQRPVAD
VQRMAGSEKHLEGWFSPAAASVQPGKLVRGLRRVALQLGVRIHEGTAMTGLEEGRPARLHTANGRVTADRVVLAMNAWMA
RAFPQFERSVAIVSSDMLITEPRPDLLQEIGLTSGVTVLDSRIFVHYYHNTPDGRIMLGKGGNTFAYGGRMLPVFDQSSP
YTGLLKRSLDDFFPAFADVKVDATWNGPSDRSVTGLPFFGQMSASGNVFYGFGYSGSGVGPCHMGGQILASLVQGLDNPW
TRSPLVNGPLGYFPPEPIRYLGSLMVRNAIRRKERAEDHGRRPRHLDVRLAKFAAAAGKADKA
>Mature_463_residues
MRPFWLEQALQLDTSEPCPPLQVDVRTDVCIVGGGYTGLWTAIMLKQQNPELDVLLIEADICGAGASGRNGGCALSWSAK
YFTLERLFGVEEAVRLVKESERSIHAIGEFCEQYGVDADYRLDGTLYTATNRAQCGSTDAVIAALERNGINSFTQRPVAD
VQRMAGSEKHLEGWFSPAAASVQPGKLVRGLRRVALQLGVRIHEGTAMTGLEEGRPARLHTANGRVTADRVVLAMNAWMA
RAFPQFERSVAIVSSDMLITEPRPDLLQEIGLTSGVTVLDSRIFVHYYHNTPDGRIMLGKGGNTFAYGGRMLPVFDQSSP
YTGLLKRSLDDFFPAFADVKVDATWNGPSDRSVTGLPFFGQMSASGNVFYGFGYSGSGVGPCHMGGQILASLVQGLDNPW
TRSPLVNGPLGYFPPEPIRYLGSLMVRNAIRRKERAEDHGRRPRHLDVRLAKFAAAAGKADKA

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=381, Percent_Identity=29.3963254593176, Blast_Score=153, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 50470; Mature: 50470

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPFWLEQALQLDTSEPCPPLQVDVRTDVCIVGGGYTGLWTAIMLKQQNPELDVLLIEAD
CCCHHHHHHHHCCCCCCCCCEEEEECCCEEEEECCHHHHHHHHHEECCCCCEEEEEEEEC
ICGAGASGRNGGCALSWSAKYFTLERLFGVEEAVRLVKESERSIHAIGEFCEQYGVDADY
CCCCCCCCCCCCEEEEECCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
RLDGTLYTATNRAQCGSTDAVIAALERNGINSFTQRPVADVQRMAGSEKHLEGWFSPAAA
EECCEEEEECCCCCCCCCHHEEEEHHHCCCCHHHCCHHHHHHHHCCCHHHHHHHCCCCCC
SVQPGKLVRGLRRVALQLGVRIHEGTAMTGLEEGRPARLHTANGRVTADRVVLAMNAWMA
CCCHHHHHHHHHHHHHHHCCEEECCCEECCCCCCCCCEEEECCCCEEHHHEEEHHHHHHH
RAFPQFERSVAIVSSDMLITEPRPDLLQEIGLTSGVTVLDSRIFVHYYHNTPDGRIMLGK
HHHHHHHHCEEEEECCEEEECCCHHHHHHCCCCCCCEEEECEEEEEEEECCCCCEEEEEC
GGNTFAYGGRMLPVFDQSSPYTGLLKRSLDDFFPAFADVKVDATWNGPSDRSVTGLPFFG
CCCEEEECCEEEEEECCCCCHHHHHHHHHHHHCCCHHCEEEEEEECCCCCCCEECCCCCC
QMSASGNVFYGFGYSGSGVGPCHMGGQILASLVQGLDNPWTRSPLVNGPLGYFPPEPIRY
CCCCCCCEEEEECCCCCCCCCHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
LGSLMVRNAIRRKERAEDHGRRPRHLDVRLAKFAAAAGKADKA
HHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRPFWLEQALQLDTSEPCPPLQVDVRTDVCIVGGGYTGLWTAIMLKQQNPELDVLLIEAD
CCCHHHHHHHHCCCCCCCCCEEEEECCCEEEEECCHHHHHHHHHEECCCCCEEEEEEEEC
ICGAGASGRNGGCALSWSAKYFTLERLFGVEEAVRLVKESERSIHAIGEFCEQYGVDADY
CCCCCCCCCCCCEEEEECCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
RLDGTLYTATNRAQCGSTDAVIAALERNGINSFTQRPVADVQRMAGSEKHLEGWFSPAAA
EECCEEEEECCCCCCCCCHHEEEEHHHCCCCHHHCCHHHHHHHHCCCHHHHHHHCCCCCC
SVQPGKLVRGLRRVALQLGVRIHEGTAMTGLEEGRPARLHTANGRVTADRVVLAMNAWMA
CCCHHHHHHHHHHHHHHHCCEEECCCEECCCCCCCCCEEEECCCCEEHHHEEEHHHHHHH
RAFPQFERSVAIVSSDMLITEPRPDLLQEIGLTSGVTVLDSRIFVHYYHNTPDGRIMLGK
HHHHHHHHCEEEEECCEEEECCCHHHHHHCCCCCCCEEEECEEEEEEEECCCCCEEEEEC
GGNTFAYGGRMLPVFDQSSPYTGLLKRSLDDFFPAFADVKVDATWNGPSDRSVTGLPFFG
CCCEEEECCEEEEEECCCCCHHHHHHHHHHHHCCCHHCEEEEEEECCCCCCCEECCCCCC
QMSASGNVFYGFGYSGSGVGPCHMGGQILASLVQGLDNPWTRSPLVNGPLGYFPPEPIRY
CCCCCCCEEEEECCCCCCCCCHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
LGSLMVRNAIRRKERAEDHGRRPRHLDVRLAKFAAAAGKADKA
HHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]