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The map label for this gene is xthA [H]

Identifier: 77459871

GI number: 77459871

Start: 4129620

End: 4130432

Strand: Direct

Name: xthA [H]

Synonym: Pfl01_3649

Alternate gene names: 77459871

Gene position: 4129620-4130432 (Clockwise)

Preceding gene: 77459863

Following gene: 77459872

Centisome position: 64.14

GC content: 59.53

Gene sequence:

>813_bases
ATGAAGATCGTTTCCTTCAACATCAACGGACTGCGGGCCCGTCCGCATCAGCTGGCGGCGCTGATCGAGAAGCATCAGCC
GGACGTGATCGGCCTGCAGGAAACCAAGGTCCACGACGACCAGTTCCCGCTGGAAGAAGTGCGGGCCCTGGGTTATCACG
TGTATTTCCACGGTCAGAAAGGTCATTACGGCGTTGCCCTGCTCTCGCGCCAGGAACCGATTGCCGTACACAAAGGTTTT
GCCACCGATGAAGAAGACGCACAGCGGCGCTTTATCTGGGGCACGTTCGCCGACGCCAACGGCGTGCCGGTGACGATCAT
GAACGGCTATTTCCCACAGGGTGAAAGCCGCGACCATCCGACCAAATTCCCGGCCAAGCAGCGCTTCTACAGCGATCTGC
AAGCGCTGCTGGAAAGCCAGTTCCACAACGAACAGCCGCTGGTGGTGATGGGCGACGTCAACATTTCCCCGGAAGACTGC
GACATCGGCATCGGCCCGGACAACATGAAGCGCTGGCTGAAAACCGGCAAATGCAGCTTCCTGCCGGAAGAGCGCGAGTG
GATGGCCCGCCTGAAAAACTGGGGCCTGACCGACAGCTTCCGCCATCTGAACCCGGACGTGACCGACACGTTCAGCTGGT
TCGACTACCGCAGCCGTGGTTTTGAAGACGAGCCGAAGCGTGGTCTGCGGATTGATGTGATTCTGGCGTCTCATGGCTTG
CTGCCGCGTGTGAAAGCCGCCGGTGTCGACTATGAACTGCGCGGGATGGAAAAGCCTTCGGACCATGCGCCGATCTGGCT
GGAATTGAGCTGA

Upstream 100 bases:

>100_bases
CCCACACCCGAAAGACATTAGCAGCGCATATGGACTACCGCCACGCTGGCCCAGATCCTACATGTCAGCTAGCATCGCCC
TTTTGCCAGGACTGCCGACC

Downstream 100 bases:

>100_bases
CCCGCTTGAAGCAGAAGATCACAGCCGGCGGTTGTGATCTTCTGTCCTGTCATCTTTCAGCAACCTCACTGACTTAATCT
CCCCGGCAATCCCCTTGGCT

Product: exonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MKIVSFNINGLRARPHQLAALIEKHQPDVIGLQETKVHDDQFPLEEVRALGYHVYFHGQKGHYGVALLSRQEPIAVHKGF
ATDEEDAQRRFIWGTFADANGVPVTIMNGYFPQGESRDHPTKFPAKQRFYSDLQALLESQFHNEQPLVVMGDVNISPEDC
DIGIGPDNMKRWLKTGKCSFLPEEREWMARLKNWGLTDSFRHLNPDVTDTFSWFDYRSRGFEDEPKRGLRIDVILASHGL
LPRVKAAGVDYELRGMEKPSDHAPIWLELS

Sequences:

>Translated_270_residues
MKIVSFNINGLRARPHQLAALIEKHQPDVIGLQETKVHDDQFPLEEVRALGYHVYFHGQKGHYGVALLSRQEPIAVHKGF
ATDEEDAQRRFIWGTFADANGVPVTIMNGYFPQGESRDHPTKFPAKQRFYSDLQALLESQFHNEQPLVVMGDVNISPEDC
DIGIGPDNMKRWLKTGKCSFLPEEREWMARLKNWGLTDSFRHLNPDVTDTFSWFDYRSRGFEDEPKRGLRIDVILASHGL
LPRVKAAGVDYELRGMEKPSDHAPIWLELS
>Mature_270_residues
MKIVSFNINGLRARPHQLAALIEKHQPDVIGLQETKVHDDQFPLEEVRALGYHVYFHGQKGHYGVALLSRQEPIAVHKGF
ATDEEDAQRRFIWGTFADANGVPVTIMNGYFPQGESRDHPTKFPAKQRFYSDLQALLESQFHNEQPLVVMGDVNISPEDC
DIGIGPDNMKRWLKTGKCSFLPEEREWMARLKNWGLTDSFRHLNPDVTDTFSWFDYRSRGFEDEPKRGLRIDVILASHGL
LPRVKAAGVDYELRGMEKPSDHAPIWLELS

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=281, Percent_Identity=30.9608540925267, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI18375503, Length=281, Percent_Identity=30.9608540925267, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI18375501, Length=281, Percent_Identity=30.9608540925267, Blast_Score=108, Evalue=6e-24,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=67.2932330827068, Blast_Score=384, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI71989536, Length=275, Percent_Identity=24.7272727272727, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI221330655, Length=274, Percent_Identity=32.4817518248175, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17136678, Length=274, Percent_Identity=32.4817518248175, Blast_Score=109, Evalue=2e-24,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30922; Mature: 30922

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00727 AP_NUCLEASE_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVSFNINGLRARPHQLAALIEKHQPDVIGLQETKVHDDQFPLEEVRALGYHVYFHGQK
CEEEEEECCCCCCCHHHHHHHHHHCCCCEECCCCCCCCCCCCCHHHHHHCEEEEEEECCC
GHYGVALLSRQEPIAVHKGFATDEEDAQRRFIWGTFADANGVPVTIMNGYFPQGESRDHP
CCEEEEEECCCCCEEEECCCCCCHHHHCEEEEEEEECCCCCCEEEEECCCCCCCCCCCCC
TKFPAKQRFYSDLQALLESQFHNEQPLVVMGDVNISPEDCDIGIGPDNMKRWLKTGKCSF
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEECCCHHHHHHHHHCCCCCC
LPEEREWMARLKNWGLTDSFRHLNPDVTDTFSWFDYRSRGFEDEPKRGLRIDVILASHGL
CCHHHHHHHHHHCCCCCCCHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCC
LPRVKAAGVDYELRGMEKPSDHAPIWLELS
CCCHHHCCCCEEECCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MKIVSFNINGLRARPHQLAALIEKHQPDVIGLQETKVHDDQFPLEEVRALGYHVYFHGQK
CEEEEEECCCCCCCHHHHHHHHHHCCCCEECCCCCCCCCCCCCHHHHHHCEEEEEEECCC
GHYGVALLSRQEPIAVHKGFATDEEDAQRRFIWGTFADANGVPVTIMNGYFPQGESRDHP
CCEEEEEECCCCCEEEECCCCCCHHHHCEEEEEEEECCCCCCEEEEECCCCCCCCCCCCC
TKFPAKQRFYSDLQALLESQFHNEQPLVVMGDVNISPEDCDIGIGPDNMKRWLKTGKCSF
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEECCCHHHHHHHHHCCCCCC
LPEEREWMARLKNWGLTDSFRHLNPDVTDTFSWFDYRSRGFEDEPKRGLRIDVILASHGL
CCHHHHHHHHHHCCCCCCCHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCC
LPRVKAAGVDYELRGMEKPSDHAPIWLELS
CCCHHHCCCCEEECCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]