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The map label for this gene is proP [H]

Identifier: 77459581

GI number: 77459581

Start: 3834364

End: 3835986

Strand: Direct

Name: proP [H]

Synonym: Pfl01_3359

Alternate gene names: 77459581

Gene position: 3834364-3835986 (Clockwise)

Preceding gene: 77459580

Following gene: 77459582

Centisome position: 59.55

GC content: 61.98

Gene sequence:

>1623_bases
ATGTCAGAACACGTTCAGCCACTGGATGCCGTGCGCAGCACGGACGCCAGCCCCGATACCCGAAAGGTCATCTTCGCCTC
GTCCCTGGGGACGGTGTTCGAGTGGTATGACTTCTTTCTCTACGGCGCCCTGGCGGCGGTGATCAGCAAGCAGTTCTTCG
CCGGGGTCAACGACACCACCGCGTTCATCTTCGCGCTGATGGCGTTCGCCGCAGGCTTCATCGTGCGGCCGTTCGGGGCG
CTGGTGTTCGGTCGGTTGGGGGACATGATCGGGCGCAAATACACGTTCCTGGCGACCATCATCCTGATGGGCGTGGCGAC
GTTCGCCGTGGGCCTGCTGCCGACCTACGCCAGCATCGGCATCGCCGCGCCGATCATTCTGGTGGTGCTGCGCATGCTGC
AGGGCCTGGCCCTCGGCGGCGAATACGGCGGCGCTGCGACTTACGTGGCGGAGCACGCGCCGATCGGCAAGCGCGGCTTC
CACACCAGTTGGATTCAATCCACTGCCACCCTCGGTCTGCTGCTGTCATTGCTGGTGGTGCTGGGTTGCCGCTACTTCAC
CGGCGATCAGTTCGAAGTCTGGGGCTGGCGCATTCCGTTCCTGCTGTCGATCGTGCTGCTGGGCATCTCCACCTGGATTC
GCCTGAGCCTGCACGAGTCGCCGGCCTATCTGAAAATGAAAGAGGAAGGCAAGGCCAGCAAGGCGCCGATCCGCGAATCC
TTCGGCAAATGGGAAAACCTCAAGGTCGTGCTGATCGCCCTGTTCAGCATCAACGCCGGCCAGGCGGTGACCTTTTATGC
GGCGCAGTTCTACGTGCTGTTCTTCCTCACCCAGTTCCTGAAAATGGACCCGGCGGTGGCCAACAGTCTGCTGATAATCA
GCGTGGTGATCGGCGCGCCGTTCTTCATCATTTTCGGTTGGCTGTCGGACAAGGTCGGGCGCAAACCGGTGCTGATGCTT
GGCTTGCTGCTGGCCACCGCGCTGTACTTCCCGATCTTCAAATCCCTGGCCCACTACGCCAACCCGGCCATCGACCACGC
CAGCCGACAGGCACCAATCACCGTGGTGGCGGATCCGGCGACCTGCACCTTCCAGTTCGATCCGGTGGGCAAGGCCAAAT
TCGACAGCCCGTGCGACAAGGTCAAGACCTTCCTGGTCAAGCAAGGCCTGCCCTACTCCAGCGTCGCCGCCCCGGCGGGC
AGCACGGTGCAAGTGAGCGTCGGCGATGTGAAACTCGACGGCTTCGACGAAGCAGCCTTACGCGGCGCCATCACCCTCGC
CGGTTATCCGCAACAGGCTGACCTGCAGCAGATCAACAAACCGATGATCGTGGCCCTGATCGTCGGCCTGATCATCATCT
CCGCCATGTGCTACGGCCCGCTCGCGGCGCTGATGGTCGAACTGTTTCCGACCCGCATCCGCTACACCTCGATGTCCCTG
CCGTATCACATTGGCAACGGCTGGTTCGGCGGTTTCCTGCCCACCGTGTCGTTTGCGCTGGTGGTGTACACCGGGGATAT
CTTCTACGGGCTGTGGTACCCGGTGCTGATTACAGGGGTGAGCCTGGTGGTGGGGATGATTTGCTTGCGTGAGACCAAGA
ATATCGACCTCGACAAGAACTGA

Upstream 100 bases:

>100_bases
GCTCTCAAAAAGGGCATATTTGATTCAAGGACATCGCGCATCACCCGGACACGCAACACCAGTGGAAACAACAAAAACAA
TCACTGTGAGGGATACCGAT

Downstream 100 bases:

>100_bases
TACATCCACTAAGCCGCTTCAAGTTAAACCTTGAAGCGGCTTTTTTTGCTGCACTTTAGTAATCGACAAATTGATGGCAC
TTCAATACCATCTTGCTTCC

Product: general substrate transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MSEHVQPLDAVRSTDASPDTRKVIFASSLGTVFEWYDFFLYGALAAVISKQFFAGVNDTTAFIFALMAFAAGFIVRPFGA
LVFGRLGDMIGRKYTFLATIILMGVATFAVGLLPTYASIGIAAPIILVVLRMLQGLALGGEYGGAATYVAEHAPIGKRGF
HTSWIQSTATLGLLLSLLVVLGCRYFTGDQFEVWGWRIPFLLSIVLLGISTWIRLSLHESPAYLKMKEEGKASKAPIRES
FGKWENLKVVLIALFSINAGQAVTFYAAQFYVLFFLTQFLKMDPAVANSLLIISVVIGAPFFIIFGWLSDKVGRKPVLML
GLLLATALYFPIFKSLAHYANPAIDHASRQAPITVVADPATCTFQFDPVGKAKFDSPCDKVKTFLVKQGLPYSSVAAPAG
STVQVSVGDVKLDGFDEAALRGAITLAGYPQQADLQQINKPMIVALIVGLIIISAMCYGPLAALMVELFPTRIRYTSMSL
PYHIGNGWFGGFLPTVSFALVVYTGDIFYGLWYPVLITGVSLVVGMICLRETKNIDLDKN

Sequences:

>Translated_540_residues
MSEHVQPLDAVRSTDASPDTRKVIFASSLGTVFEWYDFFLYGALAAVISKQFFAGVNDTTAFIFALMAFAAGFIVRPFGA
LVFGRLGDMIGRKYTFLATIILMGVATFAVGLLPTYASIGIAAPIILVVLRMLQGLALGGEYGGAATYVAEHAPIGKRGF
HTSWIQSTATLGLLLSLLVVLGCRYFTGDQFEVWGWRIPFLLSIVLLGISTWIRLSLHESPAYLKMKEEGKASKAPIRES
FGKWENLKVVLIALFSINAGQAVTFYAAQFYVLFFLTQFLKMDPAVANSLLIISVVIGAPFFIIFGWLSDKVGRKPVLML
GLLLATALYFPIFKSLAHYANPAIDHASRQAPITVVADPATCTFQFDPVGKAKFDSPCDKVKTFLVKQGLPYSSVAAPAG
STVQVSVGDVKLDGFDEAALRGAITLAGYPQQADLQQINKPMIVALIVGLIIISAMCYGPLAALMVELFPTRIRYTSMSL
PYHIGNGWFGGFLPTVSFALVVYTGDIFYGLWYPVLITGVSLVVGMICLRETKNIDLDKN
>Mature_539_residues
SEHVQPLDAVRSTDASPDTRKVIFASSLGTVFEWYDFFLYGALAAVISKQFFAGVNDTTAFIFALMAFAAGFIVRPFGAL
VFGRLGDMIGRKYTFLATIILMGVATFAVGLLPTYASIGIAAPIILVVLRMLQGLALGGEYGGAATYVAEHAPIGKRGFH
TSWIQSTATLGLLLSLLVVLGCRYFTGDQFEVWGWRIPFLLSIVLLGISTWIRLSLHESPAYLKMKEEGKASKAPIRESF
GKWENLKVVLIALFSINAGQAVTFYAAQFYVLFFLTQFLKMDPAVANSLLIISVVIGAPFFIIFGWLSDKVGRKPVLMLG
LLLATALYFPIFKSLAHYANPAIDHASRQAPITVVADPATCTFQFDPVGKAKFDSPCDKVKTFLVKQGLPYSSVAAPAGS
TVQVSVGDVKLDGFDEAALRGAITLAGYPQQADLQQINKPMIVALIVGLIIISAMCYGPLAALMVELFPTRIRYTSMSLP
YHIGNGWFGGFLPTVSFALVVYTGDIFYGLWYPVLITGVSLVVGMICLRETKNIDLDKN

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=325, Percent_Identity=36.9230769230769, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI1788292, Length=323, Percent_Identity=37.4613003095975, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1788942, Length=346, Percent_Identity=33.5260115606936, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1789941, Length=355, Percent_Identity=34.9295774647887, Blast_Score=145, Evalue=7e-36,
Organism=Escherichia coli, GI87082404, Length=324, Percent_Identity=25.9259259259259, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI87082231, Length=238, Percent_Identity=26.890756302521, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 58674; Mature: 58543

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEHVQPLDAVRSTDASPDTRKVIFASSLGTVFEWYDFFLYGALAAVISKQFFAGVNDTT
CCCCCCHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
AFIFALMAFAAGFIVRPFGALVFGRLGDMIGRKYTFLATIILMGVATFAVGLLPTYASIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAAPIILVVLRMLQGLALGGEYGGAATYVAEHAPIGKRGFHTSWIQSTATLGLLLSLLVV
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LGCRYFTGDQFEVWGWRIPFLLSIVLLGISTWIRLSLHESPAYLKMKEEGKASKAPIRES
HCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHEEEECCCCCEEEECCCCCCCCCCHHHH
FGKWENLKVVLIALFSINAGQAVTFYAAQFYVLFFLTQFLKMDPAVANSLLIISVVIGAP
CCCCCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCH
FFIIFGWLSDKVGRKPVLMLGLLLATALYFPIFKSLAHYANPAIDHASRQAPITVVADPA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEECCC
TCTFQFDPVGKAKFDSPCDKVKTFLVKQGLPYSSVAAPAGSTVQVSVGDVKLDGFDEAAL
EEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHCCCCCCEEEEEECCEEECCCCHHHH
RGAITLAGYPQQADLQQINKPMIVALIVGLIIISAMCYGPLAALMVELFPTRIRYTSMSL
HCEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECC
PYHIGNGWFGGFLPTVSFALVVYTGDIFYGLWYPVLITGVSLVVGMICLRETKNIDLDKN
CEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SEHVQPLDAVRSTDASPDTRKVIFASSLGTVFEWYDFFLYGALAAVISKQFFAGVNDTT
CCCCCHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
AFIFALMAFAAGFIVRPFGALVFGRLGDMIGRKYTFLATIILMGVATFAVGLLPTYASIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAAPIILVVLRMLQGLALGGEYGGAATYVAEHAPIGKRGFHTSWIQSTATLGLLLSLLVV
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LGCRYFTGDQFEVWGWRIPFLLSIVLLGISTWIRLSLHESPAYLKMKEEGKASKAPIRES
HCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHEEEECCCCCEEEECCCCCCCCCCHHHH
FGKWENLKVVLIALFSINAGQAVTFYAAQFYVLFFLTQFLKMDPAVANSLLIISVVIGAP
CCCCCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCH
FFIIFGWLSDKVGRKPVLMLGLLLATALYFPIFKSLAHYANPAIDHASRQAPITVVADPA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEECCC
TCTFQFDPVGKAKFDSPCDKVKTFLVKQGLPYSSVAAPAGSTVQVSVGDVKLDGFDEAAL
EEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCHHCCCCCCEEEEEECCEEECCCCHHHH
RGAITLAGYPQQADLQQINKPMIVALIVGLIIISAMCYGPLAALMVELFPTRIRYTSMSL
HCEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECC
PYHIGNGWFGGFLPTVSFALVVYTGDIFYGLWYPVLITGVSLVVGMICLRETKNIDLDKN
CEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]