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The map label for this gene is alkJ [H]

Identifier: 77459580

GI number: 77459580

Start: 3832564

End: 3834216

Strand: Direct

Name: alkJ [H]

Synonym: Pfl01_3358

Alternate gene names: 77459580

Gene position: 3832564-3834216 (Clockwise)

Preceding gene: 77459577

Following gene: 77459581

Centisome position: 59.53

GC content: 63.52

Gene sequence:

>1653_bases
ATGCAGACTTCCCTCGATGAATTCGACTACATCGTGGTCGGCGCCGGACCGGCCGGGTGTTTGCTGGCCAATCGGCTGTC
GGCCGATCCACAGCAGCGCGTGCTGTTGCTGGAAGCCGGCGGGCGCGACAACTATCCGTGGATTCACATCCCCGTGGGTT
ACCTGTTCTGCATCGGCAACCCGCGCACCGACTGGTGCTTCAAGACCGAAGAACAACCGGGCCTCAATGGCCGCGCGCTG
AGTTATCCGCGCGGCAAGGTGCTGGGCGGCTGTTCGTCGATCAACGGCATGATCTACATGCGCGGCCAGGCCAACGACTA
CGACGGCTGGGCTGCGGAGGGCAATCCGGGCTGGGCCTGGAATGACGTGCTGCCGCTGTTCAAGCAGAGCGAAAACCACT
TTGCCGGCGCGGCGGACTTTCATGGCGACAAGGGTGAGTGGCGAGTCGAACGCCAGCGCCTGTCGTGGCCGATTCTCGAT
GCCTTCCGCAGCGCTGCCGAACAGAGCGGCATCGCCAGCATCGATGACTTCAACCAGGGCGACAACGAGGGTTGCGGCTA
CTTTCAGGTCAATCAGAAGGCCGGGGTGCGCTGGAATGCGGCGAAGGCGTTTCTGAAGCCGATTCGCGATCGGGCCAATC
TCACGGTGTTGACCGACATCGAGGTGGATCGAGTGCTGTTCGAGGACGGTCGCGCTTCGAAAGTCAGTGCCCGTTGGCAG
GGTCAACAGAAGAATTTCAAGGCGCGCAAGGAAATCGTGTTGTGCGCCGGCTCCGTGGGCTCGCCGAGCATTCTGCAACG
CTCCGGAATCGGGCCGCGTCCGCTGCTGGAAAAGCTCGGCATCGGCGTGGTGCATGAATTGCCGGGCGTCGGCGGCAACC
TGCAGGATCACCTGCAACTGCGCCTGATCTACAAACTGGAAAACGCCCGCACCCTCAACCAGATCGCCGGTAGCGTGTGG
GGCAAGATGGGCATGGGCCTGCGTTATCTGTATGACCGCAGCGGGCCGTTGTCGATGGCGCCGAGTCAGCTGGGCGCGTT
TGCCCGCTCGGGGCCGGAACAGACCTCCGCCAACCTCGAATATCACGTGCAGCCGCTGTCGCTGGAGCGCTTTGGCGAAC
CGTTGCACAGCTTCCCGGCGTTCACCGCATCGGTGTGTGATTTACGGCCGCAGAGCCGGGGCCGGGTCGAGATCCGTTCC
GCCGATCCGCAAGCAGCGCCGCTGATCCAGCCCAATTACCTCAGCCATCCCGAAGACCTGCGCGTGGCCGCCGATGCGAT
TCGCCTGACCCGGCGCATCGTCAGCGCCCCGGCCCTGCAAGCTTTCAACCCGGTGGAGTACCTGCCGGGCGCCAGCCTGC
AGACCGAAGAACAACTGCACGCAGCGGCGGCGAAGATCGGCACGACGATTTTTCACCCGGTCGGCACCTGCCGCATGGGC
AACGATGCGGACGCGGTGGTCGACGCACAGTTGCGCGTGCACGGCGTACCGGGGCTGCGGATTGCCGATGCTTCGATCAT
GCCGCGCATCACCTCCGGCAACACTTGTTCGCCTACGCTGATGATTGCCGAGAAAGCGGCACAGCTGATTCTCAACCCCG
CAACTATAAACACAACCCCTGTGGGAGCTGGCTTGCCAGCGATGACGGAGTGA

Upstream 100 bases:

>100_bases
TGTTTGAAAACGCACAGCGGCTGCGCAAAAACTAACATTCTTTTGACGAAAGCTAACAACTAGGATGACCGACAACAAAA
ACAATAAGCGAGGTCAGGGA

Downstream 100 bases:

>100_bases
CATTCAACAATCATGTCGGCTGATTAACCGCCATTGCCAGCAGGCTGGCTCTCAAAAAGGGCATATTTGATTCAAGGACA
TCGCGCATCACCCGGACACG

Product: glucose-methanol-choline oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MQTSLDEFDYIVVGAGPAGCLLANRLSADPQQRVLLLEAGGRDNYPWIHIPVGYLFCIGNPRTDWCFKTEEQPGLNGRAL
SYPRGKVLGGCSSINGMIYMRGQANDYDGWAAEGNPGWAWNDVLPLFKQSENHFAGAADFHGDKGEWRVERQRLSWPILD
AFRSAAEQSGIASIDDFNQGDNEGCGYFQVNQKAGVRWNAAKAFLKPIRDRANLTVLTDIEVDRVLFEDGRASKVSARWQ
GQQKNFKARKEIVLCAGSVGSPSILQRSGIGPRPLLEKLGIGVVHELPGVGGNLQDHLQLRLIYKLENARTLNQIAGSVW
GKMGMGLRYLYDRSGPLSMAPSQLGAFARSGPEQTSANLEYHVQPLSLERFGEPLHSFPAFTASVCDLRPQSRGRVEIRS
ADPQAAPLIQPNYLSHPEDLRVAADAIRLTRRIVSAPALQAFNPVEYLPGASLQTEEQLHAAAAKIGTTIFHPVGTCRMG
NDADAVVDAQLRVHGVPGLRIADASIMPRITSGNTCSPTLMIAEKAAQLILNPATINTTPVGAGLPAMTE

Sequences:

>Translated_550_residues
MQTSLDEFDYIVVGAGPAGCLLANRLSADPQQRVLLLEAGGRDNYPWIHIPVGYLFCIGNPRTDWCFKTEEQPGLNGRAL
SYPRGKVLGGCSSINGMIYMRGQANDYDGWAAEGNPGWAWNDVLPLFKQSENHFAGAADFHGDKGEWRVERQRLSWPILD
AFRSAAEQSGIASIDDFNQGDNEGCGYFQVNQKAGVRWNAAKAFLKPIRDRANLTVLTDIEVDRVLFEDGRASKVSARWQ
GQQKNFKARKEIVLCAGSVGSPSILQRSGIGPRPLLEKLGIGVVHELPGVGGNLQDHLQLRLIYKLENARTLNQIAGSVW
GKMGMGLRYLYDRSGPLSMAPSQLGAFARSGPEQTSANLEYHVQPLSLERFGEPLHSFPAFTASVCDLRPQSRGRVEIRS
ADPQAAPLIQPNYLSHPEDLRVAADAIRLTRRIVSAPALQAFNPVEYLPGASLQTEEQLHAAAAKIGTTIFHPVGTCRMG
NDADAVVDAQLRVHGVPGLRIADASIMPRITSGNTCSPTLMIAEKAAQLILNPATINTTPVGAGLPAMTE
>Mature_550_residues
MQTSLDEFDYIVVGAGPAGCLLANRLSADPQQRVLLLEAGGRDNYPWIHIPVGYLFCIGNPRTDWCFKTEEQPGLNGRAL
SYPRGKVLGGCSSINGMIYMRGQANDYDGWAAEGNPGWAWNDVLPLFKQSENHFAGAADFHGDKGEWRVERQRLSWPILD
AFRSAAEQSGIASIDDFNQGDNEGCGYFQVNQKAGVRWNAAKAFLKPIRDRANLTVLTDIEVDRVLFEDGRASKVSARWQ
GQQKNFKARKEIVLCAGSVGSPSILQRSGIGPRPLLEKLGIGVVHELPGVGGNLQDHLQLRLIYKLENARTLNQIAGSVW
GKMGMGLRYLYDRSGPLSMAPSQLGAFARSGPEQTSANLEYHVQPLSLERFGEPLHSFPAFTASVCDLRPQSRGRVEIRS
ADPQAAPLIQPNYLSHPEDLRVAADAIRLTRRIVSAPALQAFNPVEYLPGASLQTEEQLHAAAAKIGTTIFHPVGTCRMG
NDADAVVDAQLRVHGVPGLRIADASIMPRITSGNTCSPTLMIAEKAAQLILNPATINTTPVGAGLPAMTE

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=536, Percent_Identity=41.2313432835821, Blast_Score=390, Evalue=1e-108,
Organism=Escherichia coli, GI1786503, Length=534, Percent_Identity=40.2621722846442, Blast_Score=365, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17532301, Length=545, Percent_Identity=33.2110091743119, Blast_Score=318, Evalue=3e-87,
Organism=Drosophila melanogaster, GI24642048, Length=582, Percent_Identity=35.3951890034364, Blast_Score=310, Evalue=2e-84,
Organism=Drosophila melanogaster, GI24642055, Length=574, Percent_Identity=35.5400696864112, Blast_Score=309, Evalue=3e-84,
Organism=Drosophila melanogaster, GI17137792, Length=558, Percent_Identity=35.3046594982079, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24642042, Length=566, Percent_Identity=35.1590106007067, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24642059, Length=569, Percent_Identity=34.9736379613357, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI45551458, Length=567, Percent_Identity=33.5097001763668, Blast_Score=282, Evalue=4e-76,
Organism=Drosophila melanogaster, GI45549471, Length=567, Percent_Identity=33.5097001763668, Blast_Score=282, Evalue=5e-76,
Organism=Drosophila melanogaster, GI18859995, Length=570, Percent_Identity=34.9122807017544, Blast_Score=280, Evalue=3e-75,
Organism=Drosophila melanogaster, GI24650267, Length=581, Percent_Identity=32.1858864027539, Blast_Score=275, Evalue=6e-74,
Organism=Drosophila melanogaster, GI24642039, Length=564, Percent_Identity=34.0425531914894, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24642051, Length=567, Percent_Identity=31.5696649029982, Blast_Score=249, Evalue=5e-66,
Organism=Drosophila melanogaster, GI18859993, Length=574, Percent_Identity=31.0104529616725, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24642035, Length=305, Percent_Identity=41.3114754098361, Blast_Score=216, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24642037, Length=301, Percent_Identity=39.5348837209302, Blast_Score=189, Evalue=3e-48,
Organism=Drosophila melanogaster, GI24645930, Length=577, Percent_Identity=30.155979202773, Blast_Score=175, Evalue=9e-44,
Organism=Drosophila melanogaster, GI24642057, Length=587, Percent_Identity=26.4054514480409, Blast_Score=135, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 59795; Mature: 59795

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTSLDEFDYIVVGAGPAGCLLANRLSADPQQRVLLLEAGGRDNYPWIHIPVGYLFCIGN
CCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEECEEEEEECC
PRTDWCFKTEEQPGLNGRALSYPRGKVLGGCSSINGMIYMRGQANDYDGWAAEGNPGWAW
CCCCCEEECCCCCCCCCCEECCCCCCEEECCCCCCCEEEEECCCCCCCCEECCCCCCCCH
NDVLPLFKQSENHFAGAADFHGDKGEWRVERQRLSWPILDAFRSAAEQSGIASIDDFNQG
HHHHHHHHCCCCCCCCCCCCCCCCCCEEEHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCC
DNEGCGYFQVNQKAGVRWNAAKAFLKPIRDRANLTVLTDIEVDRVLFEDGRASKVSARWQ
CCCCCCEEEECCCCCCEEHHHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCEEEHCCC
GQQKNFKARKEIVLCAGSVGSPSILQRSGIGPRPLLEKLGIGVVHELPGVGGNLQDHLQL
CCCCCCCCCEEEEEEECCCCCCHHHHHCCCCCHHHHHHCCCCEEECCCCCCCCHHHCEEE
RLIYKLENARTLNQIAGSVWGKMGMGLRYLYDRSGPLSMAPSQLGAFARSGPEQTSANLE
EEEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCEE
YHVQPLSLERFGEPLHSFPAFTASVCDLRPQSRGRVEIRSADPQAAPLIQPNYLSHPEDL
EEEEECCHHHHCCHHHHCCCHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHH
RVAADAIRLTRRIVSAPALQAFNPVEYLPGASLQTEEQLHAAAAKIGTTIFHPVGTCRMG
HHHHHHHHHHHHHHHCCHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEECC
NDADAVVDAQLRVHGVPGLRIADASIMPRITSGNTCSPTLMIAEKAAQLILNPATINTTP
CCCCEEEEEEEEEECCCCCEEECCCCCCEECCCCCCCCHHEEHHHHHHHEECCCCCCCCC
VGAGLPAMTE
CCCCCCCCCC
>Mature Secondary Structure
MQTSLDEFDYIVVGAGPAGCLLANRLSADPQQRVLLLEAGGRDNYPWIHIPVGYLFCIGN
CCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEECEEEEEECC
PRTDWCFKTEEQPGLNGRALSYPRGKVLGGCSSINGMIYMRGQANDYDGWAAEGNPGWAW
CCCCCEEECCCCCCCCCCEECCCCCCEEECCCCCCCEEEEECCCCCCCCEECCCCCCCCH
NDVLPLFKQSENHFAGAADFHGDKGEWRVERQRLSWPILDAFRSAAEQSGIASIDDFNQG
HHHHHHHHCCCCCCCCCCCCCCCCCCEEEHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCC
DNEGCGYFQVNQKAGVRWNAAKAFLKPIRDRANLTVLTDIEVDRVLFEDGRASKVSARWQ
CCCCCCEEEECCCCCCEEHHHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCEEEHCCC
GQQKNFKARKEIVLCAGSVGSPSILQRSGIGPRPLLEKLGIGVVHELPGVGGNLQDHLQL
CCCCCCCCCEEEEEEECCCCCCHHHHHCCCCCHHHHHHCCCCEEECCCCCCCCHHHCEEE
RLIYKLENARTLNQIAGSVWGKMGMGLRYLYDRSGPLSMAPSQLGAFARSGPEQTSANLE
EEEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCEE
YHVQPLSLERFGEPLHSFPAFTASVCDLRPQSRGRVEIRSADPQAAPLIQPNYLSHPEDL
EEEEECCHHHHCCHHHHCCCHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHH
RVAADAIRLTRRIVSAPALQAFNPVEYLPGASLQTEEQLHAAAAKIGTTIFHPVGTCRMG
HHHHHHHHHHHHHHHCCHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEECC
NDADAVVDAQLRVHGVPGLRIADASIMPRITSGNTCSPTLMIAEKAAQLILNPATINTTP
CCCCEEEEEEEEEECCCCCEEECCCCCCEECCCCCCCCHHEEHHHHHHHEECCCCCCCCC
VGAGLPAMTE
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]