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The map label for this gene is lcfB [H]

Identifier: 77459572

GI number: 77459572

Start: 3824533

End: 3826014

Strand: Direct

Name: lcfB [H]

Synonym: Pfl01_3350

Alternate gene names: 77459572

Gene position: 3824533-3826014 (Clockwise)

Preceding gene: 77459571

Following gene: 77459573

Centisome position: 59.4

GC content: 64.1

Gene sequence:

>1482_bases
ATGTCGCTTGAACGTCAACGATTCCAGGAAACCCTGCGCAGCCATGCAGGGCGCAATGACAACGCTTTGGCATTGTGGGG
CGACACCCTGAAAGTCAGTTACACGACGCTGTACGCCGAAGTAATGTACCGCCAGCAACGCTTGCGCGATGAAAACGTGA
AGGTTATCGCCCTGGCGCTGGACAACGGCGTCGAAGCCATACTCTGGGATCTGGCGGCGTTGTTTGAAGGCTTGACGTGC
GTGACGCTGCCGCCGTTCTTCAGTCCGGCACAACGTGCCCATTGCCTGGAACAAAGCCAGGCCGAACGGGTGATCGCTGA
ACCGGAGTTGGAGAGCGAACTGCTCGCCGCCGGTTATGAACGACGCGGTGAATTCTGGTGCCGCGCTTTTGAAGGCCCGA
ACCGCCTGCCAGTCGGCGCCGCCAAACTGACGTTTACCTCCGGCACCACCGGTACACCGAAGGGCGTGTGCCTGAGCGCC
GACAGTCTGCTGCGGGTCGCACGGGAGCTGGAAGAGGCCAGCAAACCCACGGCCCCCCAACATCACGTGGCGCTACTGCC
GCTGGCGATCCTGCTGGAGAACCTCGGCTGTTACGCCGCACTGTACGCCGGTGCGACGCTGAGCGTGCCGAGCCAGAAAA
CCCTGGGCATCCAGGGCGCCAGCGGCGTCGACCTGCCGCGCTTGCTCGGCTGTCTGGCCAGCCGGGCACCCGAGAGCCTG
ATTCTGGTGCCGCAATTGCTGCTGTTACTGGTCAGCGCTGCCGAACAGAAAGCCTTCGATCCGCAATCGCTGCGTTTTGC
CGCCGTCGGTGGCGCACGGGTTTCCGAGGATCTGCTGCACCGCGCGCAACGGGTCGGCCTGCCGGTCTACGAAGGCTACG
GCCTGTCCGAGTGCGCCTCGGTGGTCTGTCTCAACCGGCCCGGGGCTCGGCGTCCGGGGAGCGTCGGGCGGCCCCTGCCC
CATGTCGACGTGCGCCTGGCCGAAGACGGTGAGGTGTTGATCAAAGGCTCGACCCTGCTCGGCTACATTGGCGAACCCGC
GTACAGCGATGAGTGGTGGCCCAGCGGCGATCTGGGCGAGTTCGACCCGGAAGGTTTTCTCTACCTCAAGGGCCGCAAGA
AGCATCAGTTCGTCACCAGTTTCGGGCGCAACGTCAACCCGGAATGGGTCGAATCCGAACTGACCCAGCGTCATCACATC
GCTCAGGCCTTTGTGTATGGCGAAGCCTTGCCGCATAACCACGCCCTGCTCTGGCCGCACCGTCCGGACTGCACCGATGC
TCAGTTGGCGGCCGCCGTCGCCGCTGCCAACGAGGCCTTGCCCGATTACGCCCAGGTGCATCACTGGACACGTCTCAAAC
AACCTTTCACGGCCGCCAACGGCCTGCTCACCGCCAACGGTCGCCCGCGCCGCGAGGCAATCGTCGCGCAGTACCGCGCC
GTGCTTATCGAATCCGCCCTTTCCGAGGAATCCGCACCATGA

Upstream 100 bases:

>100_bases
TGGGTGCACGTGGGCAATATCCGCGCCGGTTTCATCCATCTGCGCAACATCGGTCTGTTCAGTCGGCTGGGATTGCCGAC
TTCTGTCGAAGGAGCCTGCC

Downstream 100 bases:

>100_bases
GTTTTTTCGACACCCTGCAAGAAGCCACGCACCAGGAACGCCACGAGCTGTTCAATCTGCCGATCATCCTCGATGCGCTG
CAAGGCAAGGTCAGCCTTGA

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSLERQRFQETLRSHAGRNDNALALWGDTLKVSYTTLYAEVMYRQQRLRDENVKVIALALDNGVEAILWDLAALFEGLTC
VTLPPFFSPAQRAHCLEQSQAERVIAEPELESELLAAGYERRGEFWCRAFEGPNRLPVGAAKLTFTSGTTGTPKGVCLSA
DSLLRVARELEEASKPTAPQHHVALLPLAILLENLGCYAALYAGATLSVPSQKTLGIQGASGVDLPRLLGCLASRAPESL
ILVPQLLLLLVSAAEQKAFDPQSLRFAAVGGARVSEDLLHRAQRVGLPVYEGYGLSECASVVCLNRPGARRPGSVGRPLP
HVDVRLAEDGEVLIKGSTLLGYIGEPAYSDEWWPSGDLGEFDPEGFLYLKGRKKHQFVTSFGRNVNPEWVESELTQRHHI
AQAFVYGEALPHNHALLWPHRPDCTDAQLAAAVAAANEALPDYAQVHHWTRLKQPFTAANGLLTANGRPRREAIVAQYRA
VLIESALSEESAP

Sequences:

>Translated_493_residues
MSLERQRFQETLRSHAGRNDNALALWGDTLKVSYTTLYAEVMYRQQRLRDENVKVIALALDNGVEAILWDLAALFEGLTC
VTLPPFFSPAQRAHCLEQSQAERVIAEPELESELLAAGYERRGEFWCRAFEGPNRLPVGAAKLTFTSGTTGTPKGVCLSA
DSLLRVARELEEASKPTAPQHHVALLPLAILLENLGCYAALYAGATLSVPSQKTLGIQGASGVDLPRLLGCLASRAPESL
ILVPQLLLLLVSAAEQKAFDPQSLRFAAVGGARVSEDLLHRAQRVGLPVYEGYGLSECASVVCLNRPGARRPGSVGRPLP
HVDVRLAEDGEVLIKGSTLLGYIGEPAYSDEWWPSGDLGEFDPEGFLYLKGRKKHQFVTSFGRNVNPEWVESELTQRHHI
AQAFVYGEALPHNHALLWPHRPDCTDAQLAAAVAAANEALPDYAQVHHWTRLKQPFTAANGLLTANGRPRREAIVAQYRA
VLIESALSEESAP
>Mature_492_residues
SLERQRFQETLRSHAGRNDNALALWGDTLKVSYTTLYAEVMYRQQRLRDENVKVIALALDNGVEAILWDLAALFEGLTCV
TLPPFFSPAQRAHCLEQSQAERVIAEPELESELLAAGYERRGEFWCRAFEGPNRLPVGAAKLTFTSGTTGTPKGVCLSAD
SLLRVARELEEASKPTAPQHHVALLPLAILLENLGCYAALYAGATLSVPSQKTLGIQGASGVDLPRLLGCLASRAPESLI
LVPQLLLLLVSAAEQKAFDPQSLRFAAVGGARVSEDLLHRAQRVGLPVYEGYGLSECASVVCLNRPGARRPGSVGRPLPH
VDVRLAEDGEVLIKGSTLLGYIGEPAYSDEWWPSGDLGEFDPEGFLYLKGRKKHQFVTSFGRNVNPEWVESELTQRHHIA
QAFVYGEALPHNHALLWPHRPDCTDAQLAAAVAAANEALPDYAQVHHWTRLKQPFTAANGLLTANGRPRREAIVAQYRAV
LIESALSEESAP

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG1022

COG function: function code I; Long-chain acyl-CoA synthetases (AMP-forming)

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI40807491, Length=432, Percent_Identity=25.6944444444444, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI57165410, Length=423, Percent_Identity=25.0591016548463, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI42794760, Length=426, Percent_Identity=26.056338028169, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI42794758, Length=426, Percent_Identity=26.056338028169, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI42794756, Length=426, Percent_Identity=26.056338028169, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI57165412, Length=423, Percent_Identity=24.5862884160757, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI156151445, Length=294, Percent_Identity=24.1496598639456, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1788107, Length=290, Percent_Identity=30.3448275862069, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1789201, Length=344, Percent_Identity=28.1976744186047, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI145693145, Length=290, Percent_Identity=28.2758620689655, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788595, Length=276, Percent_Identity=27.1739130434783, Blast_Score=66, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17510401, Length=420, Percent_Identity=26.9047619047619, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17541856, Length=433, Percent_Identity=24.4803695150115, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17556552, Length=431, Percent_Identity=23.8979118329466, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17558820, Length=306, Percent_Identity=26.797385620915, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI32563687, Length=305, Percent_Identity=25.9016393442623, Blast_Score=78, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17559526, Length=359, Percent_Identity=24.2339832869081, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17564090, Length=441, Percent_Identity=23.8095238095238, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17560308, Length=307, Percent_Identity=24.4299674267101, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=285, Percent_Identity=25.6140350877193, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24666501, Length=596, Percent_Identity=23.8255033557047, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24666497, Length=596, Percent_Identity=23.8255033557047, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI281366413, Length=596, Percent_Identity=24.1610738255034, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21355181, Length=290, Percent_Identity=28.9655172413793, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24581924, Length=301, Percent_Identity=25.9136212624585, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648260, Length=285, Percent_Identity=25.9649122807018, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 53854; Mature: 53723

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLERQRFQETLRSHAGRNDNALALWGDTLKVSYTTLYAEVMYRQQRLRDENVKVIALAL
CCCCHHHHHHHHHHHCCCCCCEEEEECCCEEEHHHHHHHHHHHHHHHCCCCCCEEEEEEE
DNGVEAILWDLAALFEGLTCVTLPPFFSPAQRAHCLEQSQAERVIAEPELESELLAAGYE
CCCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
RRGEFWCRAFEGPNRLPVGAAKLTFTSGTTGTPKGVCLSADSLLRVARELEEASKPTAPQ
HCCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCEEECHHHHHHHHHHHHHHCCCCCCC
HHVALLPLAILLENLGCYAALYAGATLSVPSQKTLGIQGASGVDLPRLLGCLASRAPESL
HHHHHHHHHHHHHHCCHHHHHHCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCE
ILVPQLLLLLVSAAEQKAFDPQSLRFAAVGGARVSEDLLHRAQRVGLPVYEGYGLSECAS
EHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHH
VVCLNRPGARRPGSVGRPLPHVDVRLAEDGEVLIKGSTLLGYIGEPAYSDEWWPSGDLGE
EEEECCCCCCCCCCCCCCCCCCEEEEECCCCEEEECCEEEEECCCCCCCCCCCCCCCCCC
FDPEGFLYLKGRKKHQFVTSFGRNVNPEWVESELTQRHHIAQAFVYGEALPHNHALLWPH
CCCCCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCC
RPDCTDAQLAAAVAAANEALPDYAQVHHWTRLKQPFTAANGLLTANGRPRREAIVAQYRA
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHCCCEEECCCCCHHHHHHHHHHH
VLIESALSEESAP
HHHHHHHHHCCCC
>Mature Secondary Structure 
SLERQRFQETLRSHAGRNDNALALWGDTLKVSYTTLYAEVMYRQQRLRDENVKVIALAL
CCCHHHHHHHHHHHCCCCCCEEEEECCCEEEHHHHHHHHHHHHHHHCCCCCCEEEEEEE
DNGVEAILWDLAALFEGLTCVTLPPFFSPAQRAHCLEQSQAERVIAEPELESELLAAGYE
CCCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
RRGEFWCRAFEGPNRLPVGAAKLTFTSGTTGTPKGVCLSADSLLRVARELEEASKPTAPQ
HCCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCEEECHHHHHHHHHHHHHHCCCCCCC
HHVALLPLAILLENLGCYAALYAGATLSVPSQKTLGIQGASGVDLPRLLGCLASRAPESL
HHHHHHHHHHHHHHCCHHHHHHCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCE
ILVPQLLLLLVSAAEQKAFDPQSLRFAAVGGARVSEDLLHRAQRVGLPVYEGYGLSECAS
EHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHH
VVCLNRPGARRPGSVGRPLPHVDVRLAEDGEVLIKGSTLLGYIGEPAYSDEWWPSGDLGE
EEEECCCCCCCCCCCCCCCCCCEEEEECCCCEEEECCEEEEECCCCCCCCCCCCCCCCCC
FDPEGFLYLKGRKKHQFVTSFGRNVNPEWVESELTQRHHIAQAFVYGEALPHNHALLWPH
CCCCCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCC
RPDCTDAQLAAAVAAANEALPDYAQVHHWTRLKQPFTAANGLLTANGRPRREAIVAQYRA
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHCCCEEECCCCCHHHHHHHHHHH
VLIESALSEESAP
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]