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The map label for this gene is dmlR [H]

Identifier: 77458938

GI number: 77458938

Start: 3129718

End: 3130689

Strand: Direct

Name: dmlR [H]

Synonym: Pfl01_2713

Alternate gene names: 77458938

Gene position: 3129718-3130689 (Clockwise)

Preceding gene: 77458935

Following gene: 77458942

Centisome position: 48.61

GC content: 57.92

Gene sequence:

>972_bases
ATGATCATTTTTCCCATTACGCTGAAAAACTTCGTCCGCACCCTACAGAATTTCGTGATCGTGAAGAAAATAGTCCGCGT
AGATGACCTTCAGGTATTCGTCACCACGGCTGCGGCCGGCAGTTTCTCCGCCGCCGCCCGATTGCTCGACATTTCTCCAG
CCTTGGCCAGCGGAGCGGTTCAGCGTCTTGAAGGCAGTCTTGGAGCACGGTTGTTCGTTCGCTCGACCCGGCGCTTGAGA
TTATCGGACGATGGCGAACGCTACCTTTCCCATGCCCGGCAGGCACTCGAAGCGCTCGAAAATGGCGAGATCGCACTAAG
TGAAGGTCGCGATGAAATCGCTGGCACGATTCGCTTGTCCATACCGTCGGATATCGGCCGAAACGTGCTGTTGCGATGGC
TCGACGAGTTTCAGCAACTGCATCCGAAGGTGCTGTTGCAGTTACGGGTCAGCGATCAGGTTGCCGACCTGGTCGGCGAA
CAACTGGATGCCAGTATCCGTTATGGTTTGCTGGCCGATTCCAGCCTCGTCTCTCTGGCCCTGGCGCCCTCAAACAGAAG
AACGGTCTGCGCCTCGCCAGACTATCTGGCCCGCCACGGCCAGCCAGCCACACCTGAAGAATTGGGCAACCATAACTGCC
TTCGATTTGTGATGGGGGAGCAGACCTACGAACGCTGGAGCTTTCATTCGGCGGACGGTGTCAGGACCGTTCAGGTGACG
GGCGACCGGGTCAGTGATGATGCCGATATCGTCCGGCGCTGGGCGGTTGCCGGTTTCGGAATCGTCTACAGGTCGAAAAT
CGATGTGCTGGATGACTTGCGCAGCGGTCGACTGGTGGAGCTTTTCCCGGCAGACGTCGGACAGCCGGCGCCGCTTCAGC
TCGTGTGTCCCCATCGCACCTCACTCACACCGGCTGTACAGGCCTTGAGAACTTTCCTGGCGCAACGATTCGAACGTTAC
GTAGCTGAATGA

Upstream 100 bases:

>100_bases
TGGGATTTGTGACAGACATGGTTCGCGCAGTGATCACGCCGGTGCCTGGGCTGATTAGACTAATGGCTAACAGCGCCGGC
GCCTGAGCGACGGCTGGGCA

Downstream 100 bases:

>100_bases
GAACCTGACGAGGCTGGGGTGCCGCGTCAGGTTCAGGCCTTTGAGCCAGACCTCAATGCTCTGACGTTTCTCTAGTACGT
CGCTGCAGGCTTGGCGCCGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MIIFPITLKNFVRTLQNFVIVKKIVRVDDLQVFVTTAAAGSFSAAARLLDISPALASGAVQRLEGSLGARLFVRSTRRLR
LSDDGERYLSHARQALEALENGEIALSEGRDEIAGTIRLSIPSDIGRNVLLRWLDEFQQLHPKVLLQLRVSDQVADLVGE
QLDASIRYGLLADSSLVSLALAPSNRRTVCASPDYLARHGQPATPEELGNHNCLRFVMGEQTYERWSFHSADGVRTVQVT
GDRVSDDADIVRRWAVAGFGIVYRSKIDVLDDLRSGRLVELFPADVGQPAPLQLVCPHRTSLTPAVQALRTFLAQRFERY
VAE

Sequences:

>Translated_323_residues
MIIFPITLKNFVRTLQNFVIVKKIVRVDDLQVFVTTAAAGSFSAAARLLDISPALASGAVQRLEGSLGARLFVRSTRRLR
LSDDGERYLSHARQALEALENGEIALSEGRDEIAGTIRLSIPSDIGRNVLLRWLDEFQQLHPKVLLQLRVSDQVADLVGE
QLDASIRYGLLADSSLVSLALAPSNRRTVCASPDYLARHGQPATPEELGNHNCLRFVMGEQTYERWSFHSADGVRTVQVT
GDRVSDDADIVRRWAVAGFGIVYRSKIDVLDDLRSGRLVELFPADVGQPAPLQLVCPHRTSLTPAVQALRTFLAQRFERY
VAE
>Mature_323_residues
MIIFPITLKNFVRTLQNFVIVKKIVRVDDLQVFVTTAAAGSFSAAARLLDISPALASGAVQRLEGSLGARLFVRSTRRLR
LSDDGERYLSHARQALEALENGEIALSEGRDEIAGTIRLSIPSDIGRNVLLRWLDEFQQLHPKVLLQLRVSDQVADLVGE
QLDASIRYGLLADSSLVSLALAPSNRRTVCASPDYLARHGQPATPEELGNHNCLRFVMGEQTYERWSFHSADGVRTVQVT
GDRVSDDADIVRRWAVAGFGIVYRSKIDVLDDLRSGRLVELFPADVGQPAPLQLVCPHRTSLTPAVQALRTFLAQRFERY
VAE

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=295, Percent_Identity=32.5423728813559, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI145693193, Length=299, Percent_Identity=30.4347826086957, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1789440, Length=255, Percent_Identity=32.156862745098, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1786401, Length=290, Percent_Identity=30.6896551724138, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1789639, Length=294, Percent_Identity=29.9319727891156, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1787128, Length=290, Percent_Identity=26.551724137931, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1787589, Length=274, Percent_Identity=26.2773722627737, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786448, Length=250, Percent_Identity=29.6, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787601, Length=146, Percent_Identity=29.4520547945205, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788706, Length=263, Percent_Identity=28.1368821292776, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI157672245, Length=177, Percent_Identity=29.9435028248588, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789173, Length=295, Percent_Identity=24.0677966101695, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790011, Length=273, Percent_Identity=24.9084249084249, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87082132, Length=307, Percent_Identity=25.4071661237785, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35784; Mature: 35784

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIFPITLKNFVRTLQNFVIVKKIVRVDDLQVFVTTAAAGSFSAAARLLDISPALASGAV
CEEEEEHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHCCHHHHHHHH
QRLEGSLGARLFVRSTRRLRLSDDGERYLSHARQALEALENGEIALSEGRDEIAGTIRLS
HHHHHHHCCEEEEECCCEEECCCCHHHHHHHHHHHHHHHCCCCEEEECCCCHHEEEEEEE
IPSDIGRNVLLRWLDEFQQLHPKVLLQLRVSDQVADLVGEQLDASIRYGLLADSSLVSLA
CCHHHHHHHHHHHHHHHHHHCHHHEEEEHHHHHHHHHHHHHHCCHHHCCEEECCCEEEEE
LAPSNRRTVCASPDYLARHGQPATPEELGNHNCLRFVMGEQTYERWSFHSADGVRTVQVT
ECCCCCCEEECCCHHHHHCCCCCCHHHHCCCCEEEEHHCCHHHHHCCCCCCCCEEEEEEE
GDRVSDDADIVRRWAVAGFGIVYRSKIDVLDDLRSGRLVELFPADVGQPAPLQLVCPHRT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCC
SLTPAVQALRTFLAQRFERYVAE
CCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIIFPITLKNFVRTLQNFVIVKKIVRVDDLQVFVTTAAAGSFSAAARLLDISPALASGAV
CEEEEEHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHCCHHHHHHHH
QRLEGSLGARLFVRSTRRLRLSDDGERYLSHARQALEALENGEIALSEGRDEIAGTIRLS
HHHHHHHCCEEEEECCCEEECCCCHHHHHHHHHHHHHHHCCCCEEEECCCCHHEEEEEEE
IPSDIGRNVLLRWLDEFQQLHPKVLLQLRVSDQVADLVGEQLDASIRYGLLADSSLVSLA
CCHHHHHHHHHHHHHHHHHHCHHHEEEEHHHHHHHHHHHHHHCCHHHCCEEECCCEEEEE
LAPSNRRTVCASPDYLARHGQPATPEELGNHNCLRFVMGEQTYERWSFHSADGVRTVQVT
ECCCCCCEEECCCHHHHHCCCCCCHHHHCCCCEEEEHHCCHHHHHCCCCCCCCEEEEEEE
GDRVSDDADIVRRWAVAGFGIVYRSKIDVLDDLRSGRLVELFPADVGQPAPLQLVCPHRT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCC
SLTPAVQALRTFLAQRFERYVAE
CCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]